• Journal of Microbiology and Biotechnology
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• v.30 no.3
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• pp.368-377
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• 2020

Enterotoxigenic Bacteroides fragilis (ETBF) is the main pathogen causing severe inflammatory diseases and colorectal cancer. Its biofilm plays a key role in the development of colorectal cancer. The objective of this study was to determine the antagonistic effects of cell-free supernatants (CFS) derived from Clostridium butyricum against the growth and biofilm of ETBF. Our data showed that C. butyricum CFS inhibited the growth of B. fragilis in planktonic culture. In addition, C. butyricum CFS exhibited an antibiofilm effect by inhibiting biofilm development, disassembling preformed biofilms and reducing the metabolic activity of cells in biofilms. Using confocal laser scanning microscopy, we found that C. butyricum CFS significantly suppressed the proteins and extracellular nucleic acids among the basic biofilm components. Furthermore, C. butyricum CFS significantly downregulated the expression of virulence- and efflux pump-related genes including ompA and bmeB3 in B. fragilis. Our findings suggest that C. butyricum can be used as biotherapeutic agent by inhibiting the growth and biofilm of ETBF.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.11a
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• pp.491-496
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• 2001

In recent years, the research and development about the new material proceed rapidly and actively in the building industry. As building structures become bigger, higher and more specialized, so does the demand for material with higher strength. In the future, we will need to research repair and rehabilitation to make high strength concrete mixed steel fibrous building safe. The carbon fiber reinforced plastic bonding method is widely used in reinforcing the existing concrete structure among the various methods. The repair of initiate loaded reinforced high-strength concrete beams mixed steel fibrous with epoxy bonded Carbon Fiber Sheets(CFS) was investigated experimentally. The CFS thickness and length were varied to assess the peel failure at the curtailment of CFS, The behaviour of the repaired beams was represented by load-longitudinal steel strain relation and failure modes were discussed. The test results indicate that CFS is very effective for strengthening the demand beams and controlling deflections of reinforced high strength concrete beams mixed steel fibrous happen diagonal crack, the increase in the number of CFS layers over two layers didn't effect the increase in the strength of beams.

• Food Science and Biotechnology
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• v.18 no.6
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• pp.1404-1410
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• 2009

Chestnut flours (CFs) were produced from 3 chestnut samples (Aydin, Kutahya, and Bursa) by 2 different methods (boiled and oven-dried: BOD-CF and freeze-dried: FD-CF). Functional properties and effects of CF addition (10, 20, and 30%) on the qualities of regular and low-fat cookie were investigated. The freeze drying significantly increased water solubility of CFs but decreased their water binding capacity. The emulsion capacity and stability of FD-CFs were higher than those of BOD-CFs. At pH 4, 6, 8, and 10 FD-CFs had better gelation properties. Spread ratio (SR) values of BOD-CF supplemented regular or low-fat cookies decreased with increasing CF levels. Hardness values of CF supplemented regular cookies were generally lower compared to control. SR and hardness values of FD-CF supplemented cookies were higher than those of BOD-CF supplemented ones. Surface colors of the cookies were darker with FD-CF addition as compared to BODCF addition. In low-fat cookies, the sample supplemented with 10% Kutahya FD-CF had the highest taste-flavor value. FDCF supplementation generally resulted in higher appearance and taste-flavor scores than BOD-CF supplementation in cookies.

• Magazine of the Korea Concrete Institute
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• v.10 no.4
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• pp.101-111
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• 1998

This study presents test results of RC beams strengthened by carbon fiber sheet(CFS), carbon fiber reinforced plastics(CFRP) or glass fiber reinforced plastics(GFRP) for increasing shear resistance. Nineteen specimens were tested, and the test was performed with different parameters including the type of strengthening materials(CFS, GFRP, CFRP), shear-strengthening methods(wing type, jacket type, strip type), strip-spacing, strengthening direction of FRP. The test results show that shear-damaged RC beams strengthened by FRP(CFS, GFRP, CFRP) have more improved the shear capacity. The mathematical model based on plastic theory was also developed to predict shear strength of shear-damaged RC beams strengthened by FRP. The predictions using the mathematical model. are agreed with the observations from the observed shear strengths for 19 test beams.

• Journal of the Korea institute for structural maintenance and inspection
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• v.2 no.4
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• pp.177-185
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• 1998

This paper deals with the flexural behaviors of R.C beams strengthened by carbon fiber sheets. The behaviors of strengthened beams which were preloaded up to 50%, 60% and 70% of the ultimate load of unstrengthened beam are compared with that of a beam which was not preloaded. The structural behaviors of strengthened beams are compared with analytical method in terms of load-strain of concrete, load-strain of steel bar, load-strain of CFS and falilure load. Four cases of analytical method are investigated according to cracked section or partially cracked section and including strain hardening effect of steel bar or not. Comparing the results of test and analysis, both are similar in terms of load-strain of concrete, and falilure load, the results of analytical method underestimate the failure load. But each results of load-strain of steel bar, load-strain of CFS near at failure is some different, thus near at failure the composite action between CFS and upper concrete is assumed to be disturbed. Consequently, the analytical method was proved to be efficient and accurate in estimating the flexural response of CFS strengthened RC beams.

• Structural Engineering and Mechanics
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• v.74 no.3
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• pp.381-394
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• 2020

The structural performance of cold-formed steel (CFS) built-up battened columns were numerically investigated in this paper. The built-up column sections were formed by connecting two-lipped channels back-to-back, with a regular spacing of battens plates, and have been investigated in the current study. Finite element models were validated with the test results reported by the authors in the companion paper. Using the validated models, the parametric study was extended, covering a wider range of overall slenderness to assess the accuracy of the current design rules in predicting the design strengths of the CFS built-up battened columns. The parameters viz., overall slenderness, different geometries, plate slenderness (b/t ratio) and yield stress were considered for this study. In total, a total of 228 finite element models were analyzed and the results obtained were compared with current design strength predicted by Effective Width Method of AISI Specifications (AISI S100:2016) and European specifications (EN1993-1-3:2006). The parametric study results indicated that the current design rules are limited in predicting the accuracy of the design strengths of CFS built-up battened columns. Therefore, a design equation was proposed for the AISI and EC3 specifications to predict the reliable design strength of the CFS Built-up battened columns and was also verified by the reliability analysis.

• Journal of Microbiology and Biotechnology
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• v.32 no.1
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• pp.56-63
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• 2022

This study aims to determine the antibiofilm effect of cell-free supernatant (CFS) of Lactobacillus brevis strains against Streptococcus mutans strains. To study the antibiofilm mechanism against S. mutans strains, antibacterial effects, cell surface properties (auto-aggregation and cell surface hydrophobicity), exopolysaccharide (EPS) production, and morphological changes were examined. The antibiofilm effect of L. brevis KCCM 202399 CFS as morphological changes were evaluated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), compared with the control treatment. Among the L. brevis strains, L. brevis KCCM 202399 showed the highest antibiofilm effect on S. mutans KCTC 5458. The antibacterial effect of L. brevis KCCM 202399 against S. mutans KCTC 5458 was investigated using the deferred method (16.00 mm). The minimum inhibitory concentration of L. brevis KCCM 202399 against S. mutans KCTC 5458 was 25.00%. Compared with the control treatment, L. brevis KCCM 202399 CFS inhibited the bacterial adhesion of S. mutans KCTC 5458 by decreasing auto-aggregation, cell surface hydrophobicity, and EPS production (45.91%, 40.51%, and 67.44%, respectively). L. brevis KCCM 202399 CFS inhibited and eradicated the S. mutans KCTC 5458 biofilm. Therefore, these results suggest that L. brevis KCCM 202399 CFS may be used to develop oral health in the probiotic industry.

• Journal of Powder Materials
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• v.29 no.5
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• pp.418-423
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• 2022

Carbon fibers (CFs) are considered promising composite materials for various applications. However, the high cost of CFs (as much as $26 per kg) limits their practical use in the automobile and energy industries. In this study, we developed a continuous stabilization process for manufacturing low-cost CFs. We employed a textile-grade polyacrylonitrile (PAN) fiber as a low-cost precursor and UV irradiation technique to shorten the thermal stabilization time. We confirmed that UV irradiation on the textile-grade PAN fibers could lower the initial thermal stabilization temperature and also lead to a higher reaction. These resulted in a shorter overall stabilization time and enhancement of the tensile properties of textile-grade PAN-based CFs. Our study found that only 70 min of stabilization time with UV irradiation was required to prepare textile-grade PAN-based low-cost CFs with a tensile strength of 2.37 ± 0.22 GPa and tensile modulus of 249 ± 5 GPa.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.11a
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• pp.355-358
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• 2003

The strengthening method with CFS(Carbon Fiber Sheet) to reinforced concrete structures has a fatal defect. Strengthened beams have been almost failed far below their ultimate strength due to interface debonding failure between the surface of concrete and CFS. The purpose of this study is to investigate the failure mechanism and failure behavior of strengthened RC beam using CFS.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.11a
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• pp.359-362
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• 2003

Carbon fiber sheet is attractive due to its good tensile strength, resistance to corrosion, and low weight. The strengthening of concrete structures with externally bonded carbon fiber sheets is increasingly being used for repair and rehabilitation of existing structures. However CFS strengthened beams break down under the service loads. As rupture strain is not reached ultimate value, reduction of the tensile strength is recommended. This study evaluate CFS tensile strength reduction factor which is required to analyze bending moment.