• Title/Summary/Keyword: CES

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Profiles of Toxin Genes and Antimicrobial Resistance of Bacillus cereus Strains Isolated from Commercial Jeotgal (시판 젓갈에서 분리한 Bacillus cereus의 독소 유전자 및 항균제 내성 분석)

  • Park, Kwon-Sam;Cho, Eui-Dong;Kim, Hee-Dai
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.6
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    • pp.870-877
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    • 2020
  • Twenty-three Bacillus cereus strain isolated from commercial jeotgal were investigated for 11 toxin genes and susceptibility to 25 different antimicrobials. The hemolytic enterotoxins hblA, hblC, and hblD were detected in 13.0%, and non-hemolytic enterotoxins nheA, nheB, and nheC were detected in 26.1%, 100%, and 100% of the isolates, respectively. The positive rates of cytK, entFM, becT, hlyII, and ces were 73.9%, 60.9%, 26.1%, 8.7%, and 0.0%, respectively. According to the disk diffusion susceptibility test, all of the strains studied were resistant to cefuroxime, followed by cefoxitin (78.3%), oxacillin (78.3%), ampicillin (69.6%), penicillin G (69.6%), and amoxicillin (65.2%). However, all the strains were susceptible to 11 other antimicrobials, including amikacin, chloramphenicol, and ciprofloxacin. The average minimum inhibitory concentrations of amoxicillin, ampicillin, and cefuroxime against B. cereus were 462.9, 235.0, and 135.0 ㎍/mL, respectively. These results highlight the need for sanitizing commercial jeotgal, and provide evidence to help reduce the risk of jeotgal contamination by antimicrobial-resistant bacteria.

Antioxidant Activities and Total Phenolic Contents of Three Legumes

  • Lee, Kyung Jun;Kim, Ga-Hee;Lee, Gi-An;Lee, Jung-Ro;Cho, Gyu-Taek;Ma, Kyung-Ho;Lee, Sookyeong
    • Korean Journal of Plant Resources
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    • v.34 no.6
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    • pp.527-535
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    • 2021
  • Legumes have been important components of the human diet. They contain not only protein, starch, and dietary fiber, but also various phenolic compounds such as flavonoids and phenolic acids. The importance of phenolic compounds to human health is well known due to their antioxidant activities. In this study, three legumes (adzuki beans, common beans, and black soybeans) frequently cultivated in Korea were evaluated for their total phenolic content (TPC) and antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azinobis (3-ethylbenzothiazoline 6-sulfonate)), and FRAP (ferric reducing antioxidant potential) assays. In addition, correlations between agricultural traits and antioxidant activities of these three legumes were analyzed. Antioxidant activities assessed by DPPH, ABTS, and FRAP assays and TPC showed wide variations among legumes types and accessions. Among the three legumes, adzuki beans showed higher TPC and antioxidant activity than the other two legumes. In correlation analysis, seed size showed negative correlations with antioxidant activities and TPC. In principal component analysis and hierarchical clustering analysis, each of the three legumes was clearly separate. Results of this study can be used as basic information for developing functional materials for each legume. They can also help us understand the overall antioxidant activity of the three legumes.

Effects of Ethyl Chloride Spray before Arteriovenous Fistula Puncture on Pain, Depression, and Noncompliance of Hemodialysis (혈액투석 환자의 동정맥루 천자 시 에틸클로라이드 스프레이 적용이 통증, 우울 및 치료 지시 불이행에 미치는 효과)

  • Shin, Chang-Min;Lee, Minju
    • Journal of muscle and joint health
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    • v.30 no.1
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    • pp.25-35
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    • 2023
  • Purpose: This study was conducted to examine the effects of ethyl chloride spray during arteriovenous fistula puncture on pain, depression, and noncompliance in hemodialysis patients. Methods: This study used a randomized controlled trial design on adults with chronic renal failure who received hemodialysis treatment through arteriovenous fistula. Ethyl chloride spray was applied to 20 subjects in the experimental group during arteriovenous fistula puncture, and a placebo spray was applied to 20 subjects in the control group; the intervention was conducted 12 times for 4 weeks. A total of 33 participants were used in the final analysis. The outcomes were measured using the face pain rating scale, Center for Epidemiological Studies Depression Scale (CES-D), and modified United States Renal Data System tools. Results: As a result of the pre-homogeneity test between the two groups, it was confirmed that the sexes were not homogeneous. Therefore, sex was treated as a covariate and analyzed. Puncture pain was significantly reduced in the experimental group (p<.001). However there was no significant difference between the groups in depression and noncompliance. Conclusion: Ethyl chloride spray was convenient and effective pain management intervention for both hemodialysis patients and medical staff.

The effects of academic stress, social network service addiction tendency, and upward social comparison on depression in nursing students (간호대학생의 학업스트레스, 소셜네트워크서비스 중독경향, 상향비교성향이 우울에 미치는 영향)

  • Park, Seungmi;Lee, Jung Lim;Yu, Soo-Young
    • The Journal of Korean Academic Society of Nursing Education
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    • v.29 no.1
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    • pp.41-50
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    • 2023
  • Purpose: The aim of this descriptive study was to identify the factors influencing depression risk among South Korean nursing students. Methods: The data were collected from nursing students attending two universities through web-based questionnaires that surveyed them about depression, academic stress, social network service (SNS) addiction tendency, and upward social comparison from August 22 to September 4, 2021. The collected data from 196 nursing students were analyzed by t-test, one-way ANOVA, Pearson's correlation coefficients, and multiple linear regression. Results: The mean score of depression (using CES-D Korean version) among nursing students was 13.91, which denotes probable depression. Concerning the variance with regard to depression among nursing students, 20.2% was explained by clinical practice period, academic stress, and upward social comparison. Conclusion: Programs to relieve academic stress and depression should be developed in a simple way and systematically provided at the organizational level so that nursing students secure sufficient support during the initial and continuing period of clinical practicums. Concomitantly, an attempt to reduce the upward social comparison should be highly considered.

Analysis and design of eccentrically loaded lightweight aggregate concrete-encased steel slender columns

  • Mostafa M.A. Mostafa
    • Structural Engineering and Mechanics
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    • v.88 no.1
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    • pp.25-42
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    • 2023
  • This paper presents a finite element (FE) simulation of eccentrically loaded lightweight aggregate concrete-encased steel (LACES) columns with H-shaped steel sections, analytical equations are also established to estimate the columns' axial and bending moment interaction capacities. The validity of the proposed models is checked by comparing the results with experimental data. Good agreements between the test and proposed models' results are found with acceptable agreements. Moreover, design parameters, including the lightweight aggregate concrete (LWAC) strength, eccentricity, column slenderness ratio, and confinement, are studied using the FE analysis, and their efficiency factors are discussed. The results show that the ultimate axial capacity of the LACES composite columns subjected to eccentric loading is negatively affected by the increase in the columns' height, but it is positively affected by the increase of the confinement. Increasing the eccentricity and columns' height reduced the columns'stiffness. In addition, the ultimate capacity of the LACES column is significantly influenced by the LWAC strength and eccentricity, where the ultimate capacity of the LACES column is significantly increased by increasing LWAC strength, and it is remarkably decreased by increasing the eccentricity. When the eccentricity changed from zero to 70 mm, the ultimate axial capacity and stiffness decreased by 67.97% and 63.56%, respectively.

Cone-beam computed tomography in endodontics: from the specific technical considerations of acquisition parameters and interpretation to advanced clinical applications

  • Nestor Rios-Osorio;Sara Quijano-Guauque;Sandra Brinez-Rodriguez;Gustavo Velasco-Flechas;Antonieta Munoz-Solis;Carlos Chavez;Rafael Fernandez-Grisales
    • Restorative Dentistry and Endodontics
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    • v.49 no.1
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    • pp.1.1-1.18
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    • 2024
  • The implementation of imaging methods that enable sensitive and specific observation of anatomical structures has been a constant in the evolution of endodontic therapy. Cone-beam computed tomography (CBCT) enables 3-dimensional (3D) spatial anatomical navigation in the 3 volumetric planes (sagittal, coronal and axial) which translates into great accuracy for the identification of endodontic pathologies/conditions. CBCT interpretation consists of 2 main components: (i) the generation of specific tasks of the image and (ii) the subsequent interpretation report. A systematic and reproducible method to review CBCT scans can improve the accuracy of the interpretation process, translating into greater precision in terms of diagnosis and planning of endodontic clinical procedures. MEDLINE (PubMed), Web of Science, Google Scholar, Embase and Scopus were searched from inception to March 2023. This narrative review addresses the theoretical concepts, elements of interpretation and applications of the CBCT scan in endodontics. In addition, the contents and rationale for reporting 3D endodontic imaging are discussed.

Construction of Heat-Inducible Expression Vector of Corynebacterium glutamicum and C. ammoniagenes: Fusion of ${\lambda}$ Operator with Promoters Isolated from C. ammoniagenes

  • Park, Jong-Uk;Jo, Jae-Hyung;Kim, Young-Ji;Chung, So-Sun;Lee, Jin-Ho;Lee, Hyune-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.18 no.4
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    • pp.639-647
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    • 2008
  • The heat-inducible expression vectors for Corynebacterium glutamicum and C. ammoniagenes were constructed by using the ${\lambda}O_L1$ and the cryptic promoters, CJ1 and CJ4 that express genes constitutively in C. ammoniagenes. Although the promoters were isolated from C. ammoniagenes, CJ1 and CJ4 were also active in C. glutamicum. To construct vectors, the $O_L1$ from the ${\lambda}P_L$ promoter was isolated and fused to the CJ1 and CJ4 promoters by recombinant PCR. The resulting artificial promoters, CJ1O and CJ4O, which have one ${\lambda}O_L1$, and CJ1OX2, which has two successive ${\lambda}O_L1$, were fused to the green fluorescent protein (GFP) gene followed by subcloning into pCES208. The expression of GFP in the corynebacteria harboring the vectors was regulated successfully by the temperature-sensitive cI857 repressor. Among them, C. ammoniagenes harboring plasmid pCJ1OX2G containing GFP fused to CJ1OX2 showed more GFP than the other ones and the expression was tightly regulated by the repressor. To construct the generally applicable expression vector using the plasmid pCJ1OX2G, the His-tag, enterokinase (EK) moiety, and the MCS were inserted in front of the GFP gene. Using the vector, the expression of pyrR from C. glutamicum was tried by temperature shift-up. The results indicated that the constructed vectors (pCeHEMG) can be successfully used in the expression and regulation of foreign genes in corynebacteria.

The Effect of Visual and Hearing Impairment on Depression and Cognitive Function in Community-dwelling Elderly : The Korean Longitudinal Study of Aging 2008 (노인의 시력 및 청력 저하가 우울 및 인지기능에 미치는 영향: 2008년 고령화연구패널조사)

  • Kim, Hee-Jeong;Kim, Bo-Hye;Kim, Ok-Soo
    • Korean Journal of Adult Nursing
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    • v.23 no.6
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    • pp.584-594
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    • 2011
  • Purpose: The purpose of this study was to identify the prevalence of visual and hearing impairment and to investigate the effect of visual and hearing impairment on depression and cognitive function in community-dwelling Korean elderly. Methods: The study population consisted of a representative community sample of 4,028 persons aged 65 and older from the Korean Longitudinal Study of Aging 2008. Depression and cognitive function were measured by CES-D 10-item scale and K-MMSE. Visual and hearing impairment were measured by 5-Likert scale. Data were analyzed using Rao-Scott ${\chi}^2$-test, simple and multiple logistic regression. Results: The prevalence of visual and hearing impairment were 37.3% and 14.0%, respectively. Of the participants, the prevalence of depression was 58.4% and cognitive impairment was 50.6%. There were significant differences in depression and cognitive function according to visual and hearing impairment. Far vision and hearing impairment were predictors for both depression and cognitive impairment even after covariates had been adjusted. Conclusion: These data suggest that visual and hearing impairment in older adults may increase their probability of experiencing depression and cognitive impairment. Thus sensory impairment should be considered a risk factor for mental health and the cognitive function of elderly.

An Economic Feasibility of Household Fuelcell System (가정용 연료전지 시스템의 경제성평가)

  • Ryu, Seunghyun;Kim, Suduk
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.138.1-138.1
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    • 2010
  • 정부는 그린홈 100만호 보급 사업에서 연료전지의 보급 목표를 2010년 200대 2011년 300대, 2012년 500대로 결정하였고 이에 따라 2012년까지 누적 보급대수가 1000대에 달할 전망이다. 본 연구에서는 현재 상업화 되어있는 국내업체의 1kW급 가정용 연료전지 시스템을 2009년에 도입되는 것으로 가정하여 경제성평가를 수행하였다. 분석의 편의상 2009년 서울 지역 난방면적 $100m^2$을 기준으로 전제하였고, 대표가정의 전기와 열수요는 CES 소형 열병합 사업 타당성 분석 프로그램(GS파워, 2006)을 활용하여 구했다. 비용의 경우 기존 보일러의 설비가격은 60만원이며 연료전지시스템의 설비가격은 1200만원이다. 다만 연료전지의 고가 소모품인 스택은 2007년 발간된 한국에너지 기술연구원의 신재생에너지 경제성평가 보고서를 인용하여 스택의 수명은 5년, 교체비용은 1000만 원이나 5년마다 30%의 비용 하락을 전제하였다. 또한, 연료전지시스템의 수명을 20년으로 가정하였으며 할인율은 5.5%를 가정하였다. 한편, 가정용 연료전지의 최적 운전방안을 찾기 위해서 기존 설비를 이용한 비용과 전기추종운전, 열추종운전의 시뮬레이션을 수행한 뒤 세가지 결과를 시간대별로 비교함으로써 최적의 시간대별 운전방식을 선택하는 복합추종운전의 비용을 분석하였다. 시뮬레이션결과, 기존 설비 이용 시 에너지 비용은 1,934,864원으로 분석된 반면 연료전지를 이용한 전기추종은 1,123,691원, 열추종은 1,180,425원, 복합추종은 1,121,174원으로 계산되었다. 한편 편익면에서는 복합추종운전시 813,690원의 편익이 발생하는 것으로 분석되었으며 B/C ratio의 결과는 0.405로 현재로서는 연료전지 시스템이 경제성이 없는 것으로 분석되었다. 따라서 정부는 연료전지의 보급목표와 민간 주도의 자생적인 시장형성을 촉진하기 위해서 단순 설치 보조금 이외에 연료전지시스템과 스택의 비용을 획기적으로 저감 시킬 수 있는 기술개발을 촉진하는 정책 병행이 필요해 보인다.

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말기암 환자 가족에 대한 호스피스 팀의 정보적 지지 제공 효과

  • Lee, Hye-Won
    • Korean Journal of Hospice Care
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    • v.1 no.1
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    • pp.34-45
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    • 2001
  • To evaluate the effect of informational support by hospice team on family caregivers of terminally ill cancer patients, 22 family caregivers of D University Hospital in Daegu city were participated. The research was conducted from Aug. 16th to Oct. 28th 2000 by using self-reported questionnaires. The instruments used in this study were the Weinert's scale of perceived social support, Spielberger's state anxiety inventory, CES-D, and Ellison and Paloutzian's spiritual well-being scale. The intervention was designed to give educational and counselling program up to 7 times within 4 weeks. Educational and counselling booklets which made by the researcher were used step by step by hospice team. The data were analysed frequency, percentage, Wilcoxon Singed Ranks Test Test with SPSS Win 10.0/PC. The results obtained from this study were as follows: 1.The perceived social support of family caregivers was significantly increased after ready planned informational support was applied by hospice team(z=-3.045, p=0.002). 2.The anxiety of family caregivers was significantly reduced after ready planned informational support was applied by hospice team(z=-3.348, p=0.001). 3.The depression of family caregivers was significantly reduced after ready planned informational support was applied by hospice team(z=-3.641, p=0.000). 4.The spiritual well-being score of family caregivers was not significantly improved after ready planned informational support was applied by hospice team(z=-0.422, p=0.673). In conclusion, the results of this study clearly suggests that the informational support provided by hospice team not only increased the family caregivers' who are caring for terminally ill cancer patients. Therefor the informational support program designed by researcher for family caregivers who are caring for terminally ill cancer patients should be utilized and expended.

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