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http://dx.doi.org/10.5657/KFAS.2020.0870

Profiles of Toxin Genes and Antimicrobial Resistance of Bacillus cereus Strains Isolated from Commercial Jeotgal  

Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
Cho, Eui-Dong (Department of Food Science and Biotechnology, Kunsan National University)
Kim, Hee-Dai (Department of Biotechnology and Biomedicine, Chungbuk Provincial College)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.6, 2020 , pp. 870-877 More about this Journal
Abstract
Twenty-three Bacillus cereus strain isolated from commercial jeotgal were investigated for 11 toxin genes and susceptibility to 25 different antimicrobials. The hemolytic enterotoxins hblA, hblC, and hblD were detected in 13.0%, and non-hemolytic enterotoxins nheA, nheB, and nheC were detected in 26.1%, 100%, and 100% of the isolates, respectively. The positive rates of cytK, entFM, becT, hlyII, and ces were 73.9%, 60.9%, 26.1%, 8.7%, and 0.0%, respectively. According to the disk diffusion susceptibility test, all of the strains studied were resistant to cefuroxime, followed by cefoxitin (78.3%), oxacillin (78.3%), ampicillin (69.6%), penicillin G (69.6%), and amoxicillin (65.2%). However, all the strains were susceptible to 11 other antimicrobials, including amikacin, chloramphenicol, and ciprofloxacin. The average minimum inhibitory concentrations of amoxicillin, ampicillin, and cefuroxime against B. cereus were 462.9, 235.0, and 135.0 ㎍/mL, respectively. These results highlight the need for sanitizing commercial jeotgal, and provide evidence to help reduce the risk of jeotgal contamination by antimicrobial-resistant bacteria.
Keywords
Antimicrobial susceptibility; Bacillus cereus; Commercial jeotgals; Minimum inhibitory concentration; Virulence genes;
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