• Journal of Environmental Science International
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• v.3 no.4
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• pp.409-416
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• 1994

This study was Performed to evaluate the contents of heavy metals in water, sediment and soil of the 7 different sampling points along the West Nakdong river, The results were as follows: the concentrations of Zn, p, Pb, Cd, Mn, Cu and As in the sediment were 197.48, 551.85, 67.01, 2.54, 491.39, 42.95 and 10.52ppm, respectively. The concentrations of Zn, p, Pb, Cd, Mn, Cu and As in the soil was 83.32, 482.89, 17.15, 1.02, 226.02, 26.15 and 7.29ppm, respectively. The concentration ratios of heavy metals In the water to the sediment were 593 - 12700 (Cd >> Cu > Zn > Mn > As > Pb) and that of the water to the soil were 152 - 5100 (Cu > Cd > Zn > Mn > As >Pb). The correlation coefficients of Cu and Pb weve high among the water, sediment and soil. Because the accumulation amounts of heavy metal in the sediment were high, the concentration of heavy metals in the sediment was higher than in soil. The correlation coefficient of heavy metals among water, sediment and soil was high (0.79 - 0.95). Key Words Distribution Characteristics, heavy metals, West Nakdong River.

• Journal of Veterinary Clinics
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• v.23 no.4
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• pp.432-436
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• 2006

The therapeutic effects and immune responses with Oidenlandiae Herba administration(17.5%, 1g/30 kg, everyday, PO) were investigated in calves(1 to 3 months old) with diarrhea from 159 Korean native calves(76 heads from A farm and 83 heads from B farm) in Jeju. The causes of 47 calves with diarrhea were coronavirus(1 head) and rotavirus(1 head), coccidium(5 heads) and bacteria(40 herds). PCV values were significantly increased 2 weeks after administration of Oldenlandiae Herba in A form, compared by those of pre-treatment(p<0.05). However RBC, TP, WBC, fibrinogen levels were not significantly changed. The significant difference were not detected in changes of AST, BUN and creatinine. The CD14+ distribution rate were increased in 1 week after administration of Oldenlandiae Herba, compared by that of pre-treatment(p<0.05), however, certain tendencies of changes were not observed in distribution rates of CD4+ CD8+ and CD19+, respectively. The rate of death was 20.1%(36/179 calves) in 2004, however, it was decreased to 12.6%(20/159 calves) by Oldenlandiae Herba administration at 2 farms from Jeju in 2005. In conclusion, the administration of Oldenlandiae Herba was effective for treatment of calves with diarrhea.

• Journal of Environmental Health Sciences
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• v.16 no.2
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• pp.83-87
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• 1990

This study was carried out to investigate the pollution of heavy metals in soil of seven stations from the upper spot (Yeungchun Dam) of Kumho River to the downstream(Gangchang Bridge). The results obtained were as follows: 1. The content of heavy metals in soil of Kumho River basin was highest at Gangchang Bridge [expresed in $\mu$g/g : Mn(246.0), Cd(1.90), Fe(551.2), Cu(108.2), Zn(86.4), Cr(80.2), respectively]. Whereas, the content of heavy metals expect for Mn, Cu was lowest at Yeungchun Dam [Cd(0.40), Fe(548.0), Zn(30.7), Cr(6.2), respectively] Also, the content of Cr, Zn was increased when the sampling areas are changed from upstream to downstream except for Hayang Bridge, and Hayang Bridge was the diverging point of the heavy metals content. 2. There were relatively correlated between Mn : FE, Cu, Zn, Cr, Fe : Cu, Zn, Cr(0.40 < $\left$\mid${r}\right$\mid$$ < 0.70), and were high correlated between Cd : Mn, Fe, Cu, Zn, Cu : Zn, Zn : Cr(0.70 < $\left$\mid${r}\right$\mid$$ < 0.90). Particularly, there was higest correlated between Cd : Cr, Cu : Cr(0.90< $\left$\mid${r}\right$\mid$$ < 1.0)

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.223-229
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• 2020

The application of plant-growth-promoting rhizobacteria (PGPR) supports the growth of plants in contaminated soil while ureolytic bacteria can immobilise heavy metals by carbonate precipitation. Thus, dual treatment with such bacteria may be beneficial for plant growth and bioremediation in contaminated soil. This study aimed to determine whether the PGPR Pseudomonas fluorescens could work in synergy with ureolytic bacteria to assist with the remediation of cadmium (Cd)- and lead (Pb)-contaminated soils. Pot experiments were conducted to grow radish plants in Cd- and Pb-contaminated soils treated with PGPR P. fluorescens and the results were compared with dual inoculation of P. fluorescens combined with ureolytic Staphylococcus epidermidis HJ2. The removal rate of the metals from the soil was more than 83% for Cd and Pb by the combined treatment compared to 17% by PGPR alone. Further, the dual treatment reduced the metal accumulation in the roots by more than 80%. The translocation factors for Cd and Pb in plant tissues in both treatments remained the same, suggesting that PGPR combined with the carbonate precipitation process does not hamper the transfer of essential metal ions into plant tissues from the soil.

• Economic and Environmental Geology
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• v.40 no.4
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• pp.347-360
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• 2007

In order to evaluate the degree and extent of heavy metal pollution and the environmental impacts of abandoned Cu mines in Goseong-gun, soils and paddies were collected from the mine area and have been analysed for heavy metal contents. The heavy metal contents were much higher in mountain soils than in paddy soils. Total content of heavy metals decreased in order of Cu>Zn>Pb>As>Cr>Cd in mountain soils whereas Zn>Pb>Cu>Cr>As>Cd in paddy soils. The extractable amount of heavy metals by 0.1/1N HCl decreased in order of Cu>Pb>Zn>As>Cd>Cr in mountain soils whereas Pb>Cu>Zn>As>Cd>Cr in paddy soils. Although the extraction ratios were highly various depending on the sampling site, their average values were in order of Cd(16%)>Pb(10%)>Cu(9%)>As(4.5%)>Zn-Cr(${\le}2.5%$). The soils investigated were enriched in heavy metals relative to the averages of earth crust as In order of $As{\ge}Cd$>Pb>Zn>Cu>Cr. Pollution index calculated from total or extractable heavy metals of soils indicated that the heavy metal pollution was restricted to mountain soils around abandoned Cu mines, especially the Samsan I mine. The metal contents of brown rice showed no significantly contaminated level as follows; As $nd{\sim}0.87mg/kg,\;Cd\;0.02{\sim}0.34mg/kg,\;Cu\;1.01{\sim}6.25mg/kg,\;Mn\;13.4{\sim}43.2mg/kg,\;Pb\;0.09{\sim}2.83mg/kg,\;and\;Zn\;16.5{\sim}79.1mg/kg$. From the extraction and dispersion properties of heavy metal with the soil pH ($4.5{\sim}7.8$), it can be deduced the conclusion that the heavy metal pollution is spreading in the study area mainly by the detrital migration of waste ore and gangue minerals rather than the dissolution and circulation of heavy metal.

• Journal of the Korean Chemical Society
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• v.35 no.6
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• pp.645-652
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• 1991

The stability constants$(K_f)$ of the complexes of some transition and post-transition metal ions (Co(Ⅱ), Ni(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Cd(Ⅱ), Pb(Ⅱ), Hg(Ⅱ)) with $N_2O_3$-donor macrocyclic ligand, 1,15-diaza-3,4 : 12,13-dibenzo-5,8,11-trioxacyclooctadecane ($NtnOdienH_4$), have been determined by potentiometry in aqueous solution at $25^{\circ}C$. Log $K_f$ values of the complexes were : Co(Ⅱ): 3.83, Ni(Ⅱ) : 4.56, Cu(Ⅱ) : 7.74, Zn(Ⅱ) : 4.98, Cd(Ⅱ) : 3.91, Pb(Ⅱ) : 6.65, and Hg(Ⅱ) : 14.87. The order of stabilities of transition metal complexes was the same as the natural order of stability proposed by Williams-Irving. In post-transition metal complexes, the order of stabilities was Cd(Ⅱ) < Pb(Ⅱ) < Hg(Ⅱ), and the covalent character in metal ion-donor atoms bonds appeared a dominant factor in the stability. In methanol solution, each metal ion forms 1 : 1 complex, while Ni(Ⅱ) ion forms both 1 : 1 and 1 : 2 complexes. It was confirmed by $^1H-$ and $^{13}C-$NMR spectral study that the nitrogen atoms in the ligand were major contributors for the complexation of post-transition metal ions with the ligand. It was shown, by elementry analysis, electrical conductivity and magnetic susceptibility measurements, and spectral analysis, that solid Cu(Ⅱ)-and Zn(Ⅱ)-complexes have a distorted octahedral and a tetrahedral structure, respectively.

• IMMUNE NETWORK
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• v.3 no.4
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• pp.287-294
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• 2003

Background: In kidney transplantation, donor specific transfusion may induce tolerance as a result of some immune regulatory cells against the graft. In organ transplantation, the immune state arises from a relationship between the immunocompromised graft and the immunocompetent host. However, a reverse immunological situation exists between the graft and the host in hematopoietic stem cell transplantation (HSCT). In addition, early IL-2 injections after an allogeneic murine HSCT have been shown to prevent lethal graft versus host disease (GVHD) due to CD4+ cells. We investigated the induction of the regulatory CD4+CD25+ cells after a transfusion of irradiated recipient cells with IL-2 into a donor. Methods: The splenocytes (SP) were obtained from 6 week-old BALB/c mice ($H-2^d$) and irradiated as a single cell suspension. The donor mice (C3H/He, $H-2^k$) received $5{\times}10^6$ irradiated SP, and 5,000 IU IL-2 injected intraperitoneally on the day prior to HSCT. The CD4+CD25+ cell populations in SP treated C3H/He were analyzed. In order to determine the in vivo effect of CD4+CD25+ cells, the lethally irradiated BALB/c were transplanted with $1{\times}10^7$ donor BM and $5{\times}10^6$ CD4+CD25+ cells. The other recipient mice received either $1{\times}10^7$ donor BM with $5{\times}10^6$ CD4+ CD25- cells or the untreated SP. The survival and GVHD was assessed daily by a clinical scoring system. Results: In the MLR assay, BALB/c SP was used as a stimulator with C3H/He SP, as a responder, with or without treatment. The inhibition of proliferation was $30.0{\pm}13%$ compared to the control. In addition, the MLR with either the CD4+CD25+ or CD4+CD25- cells, which were isolated by MidiMacs, from the C3H/He SP treated with the recipient SP and IL-2 was evaluated. The donor SP treated with the recipient cells and IL-2 contained more CD4+CD25+ cells ($5.4{\pm}1.5%$) than the untreated mice SP ($1.4{\pm}0.3%$)(P<0.01). There was a profound inhibition in the CD4+CD25+ cells ($61.1{\pm}6.1%$), but a marked proliferation in the CD4+CD25- cells ($129.8{\pm}65.2%$). Mice in the CD4+CD25+ group showed low GVHD scores and a slow progression from the post-HSCT day 4 to day 9, but those in the control and CD4+CD25- groups had a high score and rapid progression (P<0.001). The probability of survival was 83.3% in the CD4+CD25+ group until post-HSC day 35 and all mice in the control and CD4+CD25- groups died on post-HSCT day 8 or 9 (P=0.0105). Conclusion: Donor graft engineering with irradiated recipient SP and IL-2 (recipient specific transfusion) can induce abundant regulatory CD4+CD25+ cells to prevent GVHD.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.83-89
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• 2001

This study was conducted to test the Cd accumulation and Cd-tolerance of Pisotithus tinctorius(Pt). Pt was isolated from Pinus thunbergii forest in Muan, Chonnam Province in 1997. Pt was cultured on MMN medium supplemented with $CdSO_4{\cdot}5H_2O$ at the final concentration of 0, 0.2, 0.5, 2, and $10{\mu}g/m{\ell}$ for 40 days. Growth rate and tolerance index of the fungus were measured every week, while Cd concentration, superoxide dismutase(SOD), and glutathione reductase(GR) of the fungus were analyzed at the end of the culturing, Pt showed growth reduction in vitro at $2{\mu}g/m{\ell}$ Cd in the medium and almost stopped growth at $10{\mu}g/m{\ell}$ Cd. Tolerance index of Pt decreased with increasing Cd concentration. Cd concentration of Pt was the highest at $10{\mu}g/m{\ell}$ Cd. Activities of SOD did not show significant difference between Cd concentrations, but GR of Pt increased at $0.5{\mu}g/m{\ell}$ Cd, and decreased at $2{\mu}g/m{\ell}$ Cd. Consequently Pt could be called Cd accumulator with a tolerance mechanism to Cd. Their tolerance to Cd were expressed through the higher production of antioxidants such as GR. Pt may be used for revegetation and decontamination of soil polluted by heavy metals.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.7
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• pp.4421-4426
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• 2013

Objective: The present study employed 5-aza-2'-deoxycytidine (5-Aza-CdR) to treat non-small cell lung cancer (NSCLC) cell line A549 to investigate the effects on proliferation and expression of the TFPI-2 gene. Methods: Proliferation was assessed by MTT assay after A549 cells were treated with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR, a specific demethylating agent, for 24, 48 and 72h. At the last time point cells were also analyzed by flow cytometry (FCM) to identify any change in their cell cycle profiles. Methylation-specific polymerase chain reaction (MSPCR), real time polymerase chain reaction(real-time PCR) and western blotting were carried out to determine TFPI-2 gene methylation status, mRNA expression and protein expression. Results: MTT assay showed that the growth of A549 cells which were treated with 5-Aza-CdR was significantly suppressed as compared with the control group (0 ${\mu}mol/L$ 5-Aza-CdR). After treatment with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h, FCM showed their proportion in G0/G1 was $69.7{\pm}0.99%$, $76.1{\pm}0.83%$, $83.8{\pm}0.35%$, $95.5{\pm}0.55%$ respectively (P<0.05), and the proportion in S was $29.8{\pm}0.43%$, $23.7{\pm}0.96%$, $15.7{\pm}0.75%$, $1.73{\pm}0.45%$, respectively (P<0.05), suggesting 5-Aza-CdR treatment induced G0/G1 phase arrest. MSPCR showed that hypermethylation in the promoter region of TFPI-2 gene was detected in control group (0 ${\mu}mol/L$ 5-Aza-CdR), and demethylation appeared after treatment with 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h. Real-time PCR showed that the expression levels of TFPI-2 gene mRNA were $1{\pm}0$, $1.49{\pm}0.14$, $1.86{\pm}0.09$ and $5.80{\pm}0.15$ (P<0.05) respectively. Western blotting analysis showed the relative expression levels of TFPI-2 protein were $0.12{\pm}0.01$, $0.23{\pm}0.02$, $0.31{\pm}0.02$, $0.62{\pm}0.03$ (P<0.05). TFPI-2 protein expression in A549 cells was gradually increased significantly with increase in the 5-Aza-CdR concentration. Conclusions: TFPI-2 gene promoter methylation results in the loss of TFPI-2 mRNA and protein expression in the non-small cell lung cancer cell line A549, and 5-Aza-CdR treatment could induce the demethylation of TFPI-2 gene promoter and restore TFPI-2 gene expression. These findings provide theoretic evidence for clinical treatment of advanced non-small cell lung cancer with the demethylation agent 5-Aza-CdR. TFPI-2 may be one molecular marker for effective treatment of advanced non-small cell lung cancer with 5-Aza-CdR.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.1
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• pp.62-68
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• 2014

This feeding trial was carried out to evaluate the effects of different dietary cadmium levels on growth and tissue cadmium content in juvenile parrotfish, Oplegnathus fasciatus, using cadmium chloride ($CdCl_2$) as the cadmium source. Fifteen fish averaging $5.5{\pm}0.06$ g (mean${\pm}$SD) were randomly distributed into each of twenty one rectangular fiber tanks of 30 L capacity. Each tank was then randomly assigned to one of three replicates of seven diets containing 0.30 ($C_0$), 21.0 ($C_{21}$), 40.7 ($C_{41}$), 83.5 ($C_{83}$), 162 ($C_{162}$), 1,387 ($C_{1,387}$) and 2,743 ($C_{2,743}$) mg cadmium/kg diet. At the end of sixteen weeks of feeding trial, weight gain (WG), specific growth rate (SGR) and feed efficiency (FE) of fish fed $C_{21}$ were significantly higher than those of fish fed $C_{83}$, $C_{162}$, $C_{1,387}$ and $C_{2,743}$ (p<0.05). Weight gain, SGR and FE of fish fed $C_0$, $C_{21}$ and $C_{41}$ were significantly higher than those of fish fed $C_{162}$, $C_{1,387}$ and $C_{2,743}$. Protein efficiency ratio of fish fed $C_0$, $C_{21}$ and $C_{41}$ were significantly higher than those of fish fed $C_{1,387}$ and $C_{2,743}$. Average survival of fish fed $C_0$, $C_{21}$, $C_{41}$ and $C_{162}$ were significantly higher than that of fish fed $C_{2,743}$. Tissue cadmium concentrations increased with cadmium content of diets. Cadmium accumulated the most in liver, followed by gill and then muscle. Muscle, gill and liver cadmium concentrations of fish fed $C_0$, $C_{21}$, $C_{41}$ and $C_{83}$ were significantly lower than those of fish fed $C_{162}$, $C_{1,387}$ and $C_{2,743}$. Based on the ANOVA results of growth performance and tissue cadmium concentrations the safe dietary cadmium level could be lower than 40.7 mg Cd/kg diet while the toxic level could be higher than 162 mg Cd/kg diet.