• Title/Summary/Keyword: CD64

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Comparison of the accuracy of neutrophil CD64 and C-reactive protein as a single test for the early detection of neonatal sepsis

  • Choo, Young-Kwang;Cho, Hyun-Seok;Seo, In-Bum;Lee, Hyeon-Soo
    • Clinical and Experimental Pediatrics
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    • v.55 no.1
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    • pp.11-17
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    • 2012
  • Purpose: Early identification of neonatal sepsis is a global issue because of limitations in diagnostic procedures. The objective of this study was to compare the diagnostic accuracy of neutrophil CD64 and C-reactive protein (CRP) as a single test for the early detection of neonatal sepsis. Methods: A prospective study enrolled newborns with documented sepsis (n=11), clinical sepsis (n=12) and control newborns (n=14). CRP, neutrophil CD64, complete blood counts and blood culture were taken at the time of the suspected sepsis for the documented or clinical group and at the time of venipuncture for laboratory tests in control newborns. Neutrophil CD64 was analyzed by flow cytometry. Results: CD64 was significantly elevated in the groups with documented or clinical sepsis, whereas CRP was not significantly increased compared with controls. For documented sepsis, CD64 and CRP had a sensitivity of 91% and 9%, a specificity of 83% and 83%, a positive predictive value of 83% and 33% and a negative predictive value of 91% and 50%, respectively, with a cutoff value of 3.0 mg/dL for CD64 and 1.0 mg/dL for CRP. The area under the receiver-operating characteristic curves for CD64 index and CRP were 0.955 and 0.527 ($P$ <0.01), respectively. Conclusion: These preliminary data show that diagnostic accuracy of CD64 is superior to CRP when measured at the time of suspected sepsis, which implies that CD64 is a more reliable marker for the early identification of neonatal sepsis as a single determination compared with CRP.

Fabrication of 64x1 linear array infrared detector using Hg1-xCdxTe (Hg1-xCdxTe를 이용한 64x1 선형 적외선 감지 소자 제작)

  • Kim, Jin-Sang;Suh, Sang-Hee
    • Journal of Sensor Science and Technology
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    • v.18 no.2
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    • pp.135-138
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    • 2009
  • $64{\times}1$ forcal plane infrared detector has been fabricated by using HgCdTe epi layer. HgCdTe was grown on GaAs substrate by using metal organic chemical vapor deposition. This paper describes key developments in the epi layer growth and device fabrication process. The performance of IR imaging system is summarized.

Bactericidal Effects of Food-borne Bacteria using Chlorine Dioxide and Electrolyzed Water (이산화염소수와 전해수를 이용한 식중독균의 살균효과)

  • Lee, Hye-Rin;Kim, Su-Jin;Bang, Woo-Suk
    • Journal of Food Hygiene and Safety
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    • v.37 no.4
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    • pp.232-237
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    • 2022
  • The present study investigated the bactericidal effects of chlorine dioxide (CD) and electrolyzed water (EW) on pathogenic bacteria, such as Bacillus cereus, Staphylococcus aureus, Salmonella Typhimurium, and Escherichia coli O157:H7, by treatment them with CD and EW, respectively, for 0, 2, 4, 6, 8, and 10 min. Additionally, the sensitivities of Gram-positive (B. cereus and S. aureus) and Gram-negative (S. Typhimurium and E. coli O157:H7) to CD and EW were compared, respectively. In CD, the D-values for B. cereus, S. aureus, S. Typhimurium, and E. coli O157:H7 were 1.85±0.64, 2.06±0.85, 2.26±0.89, and 2.59±0.40 min, respectively. In EW, the D-values for B. cereus, S. aureus, S. Typhimurium, and E. coli O157:H7 were 2.13±0.32, 1.64±0.64, 1.71±0.32, and 1.86±0.36 min, respectively. All strains decreased consistently for 10 min in both CD and EW. However, the D-values of each bacterial species did not differ significantly between CD and EW (P>0.05). When comparing the bactericidal effect of CD and EW, no difference in D-value was observed, even though the pH and available chlorine concentration of CD were significantly lower than those of EW. These data could be used for the application of CD and EW in the food industry, considering characteristics such as the selection of optimal disinfectants, determination of optimal concentrations, and sensitivity to disinfection targets.

A Wide Range PLL for 64X CD-ROMs & l0X DVD-ROMs (64배속 CD-ROM 및 10배속 DVD-ROM용 광대역 위상 고정 루프)

  • 진우강;이재신;최동명;이건상;김석기
    • Proceedings of the IEEK Conference
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    • 1999.11a
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    • pp.340-343
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    • 1999
  • In this paper, we propose a wide range PLL(Phase Locked Loop) for 64X CD-ROMs & l0X DVD-ROMs. The frequency locking range of the Proposed PLL is 75MHz~370MHz. To reduce jitters caused by large VCO gain and supply voltage noise, a new V-I converter and a differential delay cell are used in 3-stage ring VCO, respectively. The new V-I converter has a 0.6V ~ 2.5V wide input range. In addition, we propose a new charge pump which has perfect current matching characteristics for the sourcing/sinking current. This new charge pump improves the locking time and the locking range of the PLL. This Chip is implemented in 0.25${\mu}{\textrm}{m}$ CMOS process. It consumes 55㎽ in worst case with a single 2.5V power supply.

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Adsorption Characteristics of the Heavy Metals, Cd(II) and Pb(II) Ions, on the Si-immobilized Sargassum horneri (실리카고정 괭생이모자반에 대한 중금속 Cd(II), Pb(II) 이온의 흡착 특성)

  • Park, Kwang-Ha;Park, Mi-A;Kim, Young-Ha
    • Analytical Science and Technology
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    • v.13 no.3
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    • pp.368-377
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    • 2000
  • Si-immobilized Sargassum horneri was used to study the adsorption characteristics along with maximum adsorption conditions of heavy metal ions, Cd(II) and Ph(II) on the Si-immobilized Sargassum horneri. More amount of Cd(II) and Pb(II) ions on the Si-immobilized Sargassum horneri than Sargassum horneri were adsorbed. And Pb(II) ions were more adsorbed in all algae than Cd(II) ions more effectively in alkaline than in acidity. Recovery ratios of Cd(II) and Pb(II) ions on the Sargassum horneri were 58.0-62.6%, 61.2-64.4% respectively, Si-immobilized Sargassum horneri 56.8-92.7%, 37.8-47.9%. Recovery ratio of Cd(II) ion was higher on the Si-immobilized Sargassum horneri but it of Pb(II) ion was lower on the Si-immobilized Sargassum horneri.

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A Study on the Construction and Use of CD-ROM Network Systems at University Libraries (대학도서관의 CD-ROM 네트워크 시스템 구축 현황 및 이용에 관한 연구)

  • Kim Soon-Won;Chung Young-Mee
    • Journal of the Korean Society for Library and Information Science
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    • v.33 no.3
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    • pp.145-167
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    • 1999
  • This study aims to identify factors to be considered in designing and operating CD-ROM network systems efficiently and provide designers and operators with basic guidelines. In this study, the current status and problems of CD-ROM network systems at 72 domestic university libraries are examined, and user behavior and satisfaction of 64 actual users in 3 universities are analyzed. In addition, to examine the influenced correlation among the factors such as user satisfaction, system operating policy, system resources and system performance, $\chi^2(Chi-Square)$ test, F test and regression analysis are carried out and the factors influencing user satisfaction are examined.

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Biosorption of Lead and Cadmium by Fucoidan from Undariafinnatifida (미역 포자엽 fucoidan의 중금속 흡착 특성)

  • KOO Jae-Geun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.521-525
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    • 2001
  • Screening tests of different fucoidan fractions from Sporophylls of Undazia pinnatifida, Laminaria religiosa, Hizikia fusiforme and Sagassum fulvellum revealed that the highest biosorptive Pb and Cd uptake fraction was Undaria finnatifida Fr-3.0 prepared by dissolving the precipitated complex (crude fucoidan and cetylpyridinum chloride) with 3.0 M $CaCl_2$ solution, The Pb and Cd uptake by Undaria finnatifida Fr-3.0 was quantitatively evaluated using sorption isotherms and Langmuir sorption model. The Pb and Cd uptake by Undaria finnatifida Fr-3.0 increased with increasing pH values at high equilibrium residual concentration. The highest experimentally observed Pb and Cd uptake value in the sorption isotherm for pH 5.5 were 94 mg/g (at $C_f=164\;mg/L$) and 64 mg/g (at $C_f=197\;mg/L$) respectively, and $q_{max}$ of Pb and Cd calculated by Langmuir sorption model were 178 mg/g and 122 mg/g, respectively. In the low equilibrium concentration range, up to 20 mg/L, the Pb uptake remained unchanged in the presence of Cd, but decreased at higher equilibrium concentration range.

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Alteration in Leukocyte Subsets and Expressions of FcγR and Complement Receptors among Female Ragpickers in Eastern India

  • Mondal, Nandan K.;Siddique, Shabana;Banerjee, Madhuchanda;Roychoudhury, Sanghita;Mukherjee, Sayali;Slaughter, Mark S.;Lahiri, Twisha;Ray, Manas R.
    • Safety and Health at Work
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    • v.8 no.2
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    • pp.198-205
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    • 2017
  • Background: There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity. Methods: Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of $Fc{\gamma}$ receptor I (CD64), $Fc{\gamma}RIII$ (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14. Serum total immunoglobulin-E was estimated with enzyme-linked immunosorbent assay. Results: Compared with the controls, ragpickers had significantly (p < 0.0001) higher levels of CD8-T-cytotoxic, CD16+CD56+natural killer, and CD4+CD45RO+memory T-cells, but depleted levels of CD19+B-cells. The percentage of CD4+T-helper-cells was lower than the control group (p < 0.0001), but their absolute number was relatively unchanged (p = 0.42) due to 11% higher lymphocyte counts in ragpickers. In ragpickers, the percentages of CD14+CD16+intermediate and CD14dim CD16+nonclassical monocyte subsets were elevated with a decline in CD14+CD16-classical monocytes. The expressions of CD64, CD16, CD35, and CD11b/CD18 on both monocytes and neutrophils, and CD14 on monocytes were significantly higher in ragpickers. In addition, ragpickers had 2.7-times more serum immunoglobulin-E than the controls (p < 0.0001). After controlling potential confounders, the profession of ragpicking was positively associated with the changes. Conclusion: Ragpicking is associated with alterations in both innate (neutrophils, monocytes, and natural killer cell numbers and expression of complement and $Fc{\gamma}$ receptors) and adaptive immunity (numbers of circulating B cells, helper, cytotoxic, and memory T cells).

Effects of $CdCl_2$ Heat Treatment on the Properties of Sputter Deposited CdS Films (스퍼터 증착된 CdS 박막의 $CdCl_2$를 이용한 열처리 효과)

  • Lee, Jae-Hyeong;Choi, Sung-Hun;Lee, Dong-Jin;Jung, Hak-Kee;Lim, Dong-Gun;Yang, Kea-Joon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.11a
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    • pp.63-64
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    • 2005
  • CdS thin films were fabricated by rf magnetron sputter technique, and annealed in tube furnace using vacuum evaporated $CdCl_2$ layer, In addition, effects of the thickness of $CdCl_2$ layer and the annealing temperature on structural and optical properties of CdS films were investigated. The heat treatment process was carried out by heating the sample in air at $350-500^{\circ}C$ for 20 minute.

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Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus

  • Kim, Mu-Chan;Park, Chan-Il
    • Fisheries and Aquatic Sciences
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    • v.8 no.2
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    • pp.56-64
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    • 2005
  • Two distinct CD3 homologue genes, $CD3\gamma/\delta\;and\;CD\varepsilon$, were isolated from a olive flounder leukocyte cDNA library and a BAC library. $CD3\gamma/\delta$ consisted of 961 bp encoding 178 amino acid residues, and $CD3\varepsilon$ consisted of 1006 bp encoding 164 amino acid residues. When compared with other known CD3 peptide sequences, the most conserved region of the two olive flounder CD3 chain peptides are the cytoplasmic domain and the least conserved are the extracellular domain. A phylogenetic analysis based on the deduced amino acid sequence grouped the two olive flounder CD3 sequences with $CD3\varepsilon$ and $CD3\gamma/\delta$, respectively. The olive flounder CD3 cluster (consisting of $CD3\varepsilon\;and\;CD3\gamma/\delta$) spans only 10.4 kb. The $CD3\varepsilon\;and\;CD3\gamma/\delta$ genes are oppositely transcribed only 3.8 kb apart. Both olive flounder CD3 genes have five exons. The two olive flounder CD3 genes were predominantly expressed in PBLs, kidney, spleen, and gills.