Safety and Health at Work

Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency (산업안전보건연구원)
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Alteration in Leukocyte Subsets and Expressions of FcγR and Complement Receptors among Female Ragpickers in Eastern India

  • Mondal, Nandan K. (Department of Experimental Hematology, Chittaranjan National Cancer Institute) ;
  • Siddique, Shabana (Department of Experimental Hematology, Chittaranjan National Cancer Institute) ;
  • Banerjee, Madhuchanda (Department of Experimental Hematology, Chittaranjan National Cancer Institute) ;
  • Roychoudhury, Sanghita (Department of Experimental Hematology, Chittaranjan National Cancer Institute) ;
  • Mukherjee, Sayali (Department of Experimental Hematology, Chittaranjan National Cancer Institute) ;
  • Slaughter, Mark S. (Department of Cardiovascular and Thoracic Surgery, Cardiovascular Innovation Institute, University of Louisville School of Medicine) ;
  • Lahiri, Twisha (Department of Experimental Hematology, Chittaranjan National Cancer Institute) ;
  • Ray, Manas R. (Department of Experimental Hematology, Chittaranjan National Cancer Institute)
  • Received : 2016.06.04
  • Accepted : 2016.10.13
  • Published : 2017.06.30
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Abstract

Background: There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity. Methods: Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of $Fc{\gamma}$ receptor I (CD64), $Fc{\gamma}RIII$ (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14. Serum total immunoglobulin-E was estimated with enzyme-linked immunosorbent assay. Results: Compared with the controls, ragpickers had significantly (p < 0.0001) higher levels of CD8-T-cytotoxic, CD16+CD56+natural killer, and CD4+CD45RO+memory T-cells, but depleted levels of CD19+B-cells. The percentage of CD4+T-helper-cells was lower than the control group (p < 0.0001), but their absolute number was relatively unchanged (p = 0.42) due to 11% higher lymphocyte counts in ragpickers. In ragpickers, the percentages of CD14+CD16+intermediate and CD14dim CD16+nonclassical monocyte subsets were elevated with a decline in CD14+CD16-classical monocytes. The expressions of CD64, CD16, CD35, and CD11b/CD18 on both monocytes and neutrophils, and CD14 on monocytes were significantly higher in ragpickers. In addition, ragpickers had 2.7-times more serum immunoglobulin-E than the controls (p < 0.0001). After controlling potential confounders, the profession of ragpicking was positively associated with the changes. Conclusion: Ragpicking is associated with alterations in both innate (neutrophils, monocytes, and natural killer cell numbers and expression of complement and $Fc{\gamma}$ receptors) and adaptive immunity (numbers of circulating B cells, helper, cytotoxic, and memory T cells).

Keywords

References

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