• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2002년도 가을 학술발표논문집 Vol.29 No.2 (1)
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• pp.637-639
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• 2002

소프트웨어 환경에서 업데이트는 버그를 수정하거나 보안패치를 하거나 새로운 기능을 추가시킨다. 그러나 기존의 업데이트는 사용자 인증을 고려하지 않고 단지 cd-key 만으로 사용자 인증을 하거나 웹 사이트에서 자유롭게 업데이트를 할 수 있다. 따라서 본 논문에서는 소프트웨어 환경에서 업데이트시 사용자와 서비스 제공자 사이에 패스워드 기반의 인증 방식을 적용하고, DRM(Digital Rights Management)에서 라이센스 개념을 도입하여 불법 사용자의 접근을 차단할 수 있는 방안을 제시한다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.II
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• pp.1390-1393
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• 2005

We fabricated an efficient top emission organic light emitting diode (FTEOLED) on paper substrates. For water proof and surface planarization, parylene of 5mm thick has been coated on copy paper substrate by vapor polymerization. As use this coating layer, fabrication of device was possible by photolithography and wet etching. Because paper is not transparent, we adapted top emission structure with transparent cathode and reflective anode. The FTOLED on paper showed the excellent electrical characteristic, $109cd/m^2$, 2.3cd/A at 10V.

• Journal of Ginseng Research
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• 제28권4호
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• pp.173-182
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• 2004

We have shown that long-term intake of Korean red ginseng (KRG) delays disease progression in HIV-I infected patients. In the present study to investigate whether this slow progression was associated with protective human leukocyte antigen (HLA) alleles as well as with KRG-intake, we have performed clinical analysis of 31 HIV-1 infected patients who have been living for more than 10 years without any antiretroviral therapy. Average amount of KRG-intake over $130\;{\pm}16$ months was $4,797\;{\pm}4,921\;g$ and the annual decrease in CD4 T cell (AD) was $30\;{\pm}29{\mu}L$. We observed significant correlations among amount of KRG-intake, AD(r=-0.53, P < 0.01), and plasma HIV-1 RNA copy (r=-0.35, P < 0.05), along with a significant correlation between KRG-intake and HLA score AD(r=-0.49, P < 0.01), whereas there was no significant correlation between HLA score and AD or viral load. When the 31 patients were divided into 2 groups based on the amount of KRG-intake, the $AD(14/{\mu}L)$ in the 16 patients who had taken higher amounts of KRG was significantly less than that $(49/{\mu}L)$ in the 15 patients with a little or no KRG-intake (P < 0.01). These data indicate that KRG-intake sig­nificantly slows CD4 T cell depletion in HIV-1 infected patients.

• 한국자기공명학회논문지
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• 제3권2호
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• pp.100-108
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• 1999

Cop protein is the transcription repressor protein in rolling circle replication plasmid. With antisense RNA, Cop protein controls the copy number of plasmid. Cop family proteins have been found in various plasmids. Among Cop family proteins, Cop studied in this paper consists of 55 amino acids (Mw. 6,400), and was known to have trimer structure. Since no structural facts are elucidated, we have carried out preliminary experiments aimed at the elucidation of its three dimensional structure. The secondary structure of Cop is studied by CD and NMR. To solve the aggregation of Cop at high concentration, we tested various detergents and salts. The addition of detergents and salts could not solve the aggregation problem. However, we found that concentration is important in solving the aggregation problem. We knew that 0.18mM in 50mM potassium phosphate without any other ingredients is maximum concentration not to aggregate. Wa also investigated the pH dependence of Cop protein, and knew that Cop protein is more stable in acid state. At various temperatures, 15N-1H HSQC spectra were measured in order to find the optimal experimental condition. To enhance the peak resolution, 3D NOESY-HSQC spectrum is acquired. Since there are NOE peaks in the NH-NH region, we knew that Cop protein has $\alpha$-helical content, which was also confirmed by CD.

• 한국가축번식학회지
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• 제27권2호
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• pp.179-185
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• 2003

본 연구는 생체 내 세포 및 조직배양기로서의 면역결핍동물을 개발할 목적으로 proximal Ick promoter와 DT-A유전자를 이용하여 형질전환생쥐를 생산하였고 형질전환생쥐의 면역기관에서 Di-phteria toxin이 발현되어 T-cell이 결핍되는지를 분석하였다. 총 암수 2마리의 형질전환생쥐를 PCR과 South-ern blotting으로 분석하여 얻었으며 이식유전자의 copy수는 약 2∼3 copy가 정착된 것으로 확인되었다. 형질전환생쥐의 thymus, spleen, liver 조직을 분리한 후 total RNA를 추출하여 poly(dT) primer 와 DT 특이적 primer를 이용하여 RT-PCR수행 결과 형질전환생쥐의 thymus, spleen, liver에서 DT gene이 발현되고 있는 것을 확인할 수 있었다. 형질전환생쥐의 이들 조직간에 DT 발현량에는 큰차이는 없는 것으로 확인되었다. 형질전환생쥐의 혈액에서 적혈구, 백혈구 ,혈소판, 헤모글로빈 등이 정상생쥐보다 감소되었고 특히 백혈구수와 혈소판의 수가 크게 감소되어 있는 것으로 나타났다. 또한 형질전환생쥐의 혈액을 CD3 antibody를 이용하여 FACS 분석을 실시하여 형질전환생쥐의 혈액 중 T-cell이 수가 비정상적으로 줄어든 것을 확인할 수 있었다. 본 연구에서는 Ick-DT 형질전환생쥐에서 DT유전자의 발현에 의한 T-cell 결핍을 유도할 수 있는 것으로 나타나 이를 바탕으로 돼지를 이용한 사람의 이종장기 배양용 형질전환동물을 생산하여 응용될 수 있을 것으로 사료된다.

• 인터넷정보학회논문지
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• 제2권4호
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• pp.101-109
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• 2001

최근의 화상 정보와 디지털 화상정보의 사용량은 무한히 커지고 있다. 하지만 이러한 화상정보는 인터넷과 CD-ROM등을 통해 수없이 많은 무단 복사와 저작권 피해 사례가 많이 발생되고 있다. 또한 워터마킹 방법에 의한 원본 데이터의 왜곡과 워터마크 데이터의 사용으로 인한 원본 데이터의 방대성 등의 문제점을 해결하고 검증을 위한 방법들이 여러 가지로 제안되고 있다. 그중 일반적으로 화상정보의 저작권 소유권에 대해서는 워터마킹 방법이 일반적으로 많이 사용되고 있다. 이런 워터마킹 방법은 화상정보의 검증 절차의 복잡성과 계산 량의 증가가 발생하는 경우가 많다. 본 논문에서는 이런 문제점을 해결할 수 있도록 정지 화상정보에 오토스테레오그램(Autosterogram)을 합성하면서 워터마킹 정보의 삽입과 추출에 있어서 발생되는 원 데이터의 변형, 왜곡을 방지하기 위해 삽입되는 정보의 양을 결정하는 scaling 파라미터를 부분적으로 최적화, 워터마크 정보 추출의 최대화, 복원 시 워터마크 정보를 극대화하는 방법에 대해 알아보았다.

• 한국도서관정보학회지
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• 제28권
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• pp.61-91
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• 1998

In contemporary education, a school library is considered and valued as a vital and integral component of a school's instructional program. Especially, school library materials should contribute to execution of school curriculums through wide provision of resources. The results of the study are as follows. 1. The term 'library materials' should be changed into 'library resources' with a broad meaning. And, the scope of school library materials should include printed materials, audio-visual materials, electronic materials, associated equipment and other library resources. 2. It is desirable that the minimal standards of total collection is 5 books and 3 AV for a student in elementary schools, 7 books and 5 AV for a student in middle schools, 10 books and 7 AV for a student in high schools. 3. Annual growth is 0.5 for a student in elementary schools, 1 for a student in middle schools and 1.5 copy for a student in high schools. And, the number of minimal serials is 20 titles in elementary schools, 30 titles in middle schools and 50 tides in high schools. 4. Besides, basic collection, with the advances of technology, the school library collection should include new formats and delivery systems such as CD, video discs, videotex, computer software, satellite transmission etc. 5. The recommendable annual ratio of withdrawl is 3 to 5% for update and functional materials.

• 한국도서관정보학회지
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• 제26권
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• pp.93-130
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• 1997

The objective of this study is to catch hold of the problem occurring in the process in which librarians have obtained government publications and to show the method that can construct the rational distribution system of government publications. For this study, total 56 librarians who work for 44 institutes sponsored by central government and 12 institutes sponsored by local government were chosen as the objects of research and questionnaire survey to know how to obtain government publications. How to construct the rational distribution system of government publications is as follows. (1) The exclusive organization to manage overall service for government publications should be established newly or previous similar organization should be reorganized. (2) As reinforcing the law concerning deposit copy, government publications should be distributed obligatorily to all of the depository agency, and the depository library system of government publications should be introduced and managed newly. Especially the management of deposit copy and the depository library system of government publications should be undertaken by the exclusive agency of government publications. (3) Free-government publications should be converted to pay-government publications as much as possible so that everyone can purchase government publications easily. And free-government publications which are difficult to convert to pay-government publications should be distributed according to rational standard by the exclusive agency of government publications. (4) Pay-government publications should be sold by private distribution organization under exclusive agency of government publications. Also printing, editing and pricing of pay-government publications should be done by private distribution organization to compete with general books. (5) In order to promote the sales of pay-government publications, the sales centers of government publications must be established even in city and county. Also special government publications which have much demand should be sold even in general book stores. Especially the management of the sales centers should be undertaken by private distribution organization. (6) It is necessary to abolish or improve unreasonable regulations or system related to government publications and to make legislative, institutional devices for government publications. (7) The public relations for government publications should be enforced much through TV or radio etc. , and the various bibliographic tools of government publications should be developed and disseminated for the sake of much utilization of government publications. (8) By using new technology like CD-ROMs or communication networks, electronic production and distribution of government publications should be push ahead positively. And electronic information distribution center to su n.0, pport it should be established by government agency.

• Nutrition Research and Practice
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• 제13권1호
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• pp.58-63
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• 2019

BACKGROUND/OBJECTIVES: Telomeres are located at the chromosomal ends and progressively shortened during each cell cycle. Telomerase, which is regulated by hTERT and c-MYC, maintains telomeric DNA sequences. Especially, telomerase is active in cancer and stem cells to maintain telomere length for replicative immortality. Recently we reported that walnut phenolic extract (WPE) can reduce cell viability in a colon cancer stem cell (CSC) model. We, therefore, investigated the effect of WPE on telomere maintenance in the same model. MATERIALS AND METHODS: $CD133^+CD44^+$ cells from HCT116, a human colon cancer cell line, were sorted by Fluorescence-activated cell sorting (FACS) and treated with WPE at the concentrations of 0, 10, 20, and $40{\mu}g/mL$ for 6 days. Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA ($ddC_t$). Telomerase activity was also measured by qRT-PCR after incubating the PCR mixture with cell protein extracts, which was adjusted with reference DNA ($dC_t$). Transcriptions of hTERT and c-MYC were determined using conventional RT-PCR. RESULTS: Telomere length of WPE-treated cells was significantly decreased in a dose-dependent manner ($5.16{\pm}0.13$ at $0{\mu}g/mL$, $4.79{\pm}0.12$ at $10{\mu}g/mL$, $3.24{\pm}0.08$ at $20{\mu}g/mL$ and $3.99{\pm}0.09$ at $40{\mu}g/mL$; P = 0.0276). Telomerase activities concurrently decreased with telomere length ($1.47{\pm}0.04$, $1.09{\pm}0.01$, $0.76{\pm}0.08$, and $0.88{\pm}0.06$; P = 0.0067). There was a positive correlation between telomere length and telomerase activity (r = 0.9090; P < 0.0001). Transcriptions of both hTERT and c-MYC were also significantly decreased in the same manner. CONCLUSION: In the present cell culture model, WPE reduced telomere maintenance, which may provide a mechanistic link to the effect of walnuts on the viability of colon CSCs.

• 대한바이러스학회지
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• 제29권2호
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• pp.107-118
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• 1999

To characterize the molecular nature of human immunodeficiency virus (HIV)-1, we determined the full-length HIV-1 sequences from cultured peripheral blood mononuclear cells (PBMC) of a Korean long-term nonprogressor (LTNP). Without antiretroviral therapy, the individual has maintained CD4+ T counts over $500/{\mu}l$ from 1989 to 1999. Plasma viral RNA copy was 992 U/ml in 1998. Culture supernatant showed positive from culture days 9. A series of 9 overlapping PCR products were amplified from cultured PBMC and cloned About 9.2 kb from R of 5' LTR to R of 3' LTR was determined by automated sequencing. The G-to-A hypermutations were shown throughout the entire region. As a result of G to A hypermutations, premature stop codon was found in integrase coding region. Though there was no recombination between subtypes over all genomes, TATA box in both LTRs was TAAAA which is detected in subtype E instead of TATAA in subtype B. And, there were nucleotide GC insertion between $NF-{\kappa}B$ I and Sp1 III, and duplication of $TCF-1{\alpha}$ in LTR. We could not find any deletion of amino acid in Nef, Gag, Pol and Env gene. This study is the first report on molecular nature of full genomes of HIV-1 isolated in Korea.