• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.36-36
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• 2022

The molecular understanding of resistance and susceptibility of host plants to scab, a most threatful disease to pome fruit production worldwide, is very limited. Comparing resistant line '93-3-98' to susceptible one 'Sweet Skin' at seven time points of 0, 0.5, 1, 2, 3, 4, 8 days post inoculation, RNA-sequencing data derived from infected and mock-inoculated young leaves were analyzed to evaluate the tolerant response and to mine candidate genes of pear to the scab pathogen Venturia nashicola. Analysis of the mapped reads showed that the infection of V. nashicola led to significant differential expression of 17,827 transcripts with more than 3-fold change in the seven pairs of libraries, of which 9,672 (54%) are up- and 8,155(46%) are down-regulated. These included mainly receptor (NB-ARC domains-containing, CC-NBS-LRR, TIR-NBS-LRR, seven transmembrane MLO family protein) and transcription factor (ethylene responsive element binding, WRKY DNA-binding protein) related gene. An arsenal of defense response of highly resistant pear accessions derived from European pear was probably supposed no sooner had V. nashicola infected its host than host genes related to disease suppression like Polyketide cyclase/dehydrase and lipid transport protein, WRKY family transcription factor, lectin protein kinase, cystein-rich RLK, calcium-dependent phospholipid-binding copine protein were greatly boosted and eradicated cascade reaction induced by pathogen within 24 hours. To identify transcripts specifically expressed in response to V. nashicola, RT-PCRs were conducted and compare to the expression patterns of seven cultivars with a range of highly resistant to highly susceptible symptom. A DEG belonging to the PR protein family genes that were higher expressed in response to V. nashicola suggesting extraordinary role in the resistance response were led to the identification. This study provides the first transcriptional profile by RNA-seq of the host plant during scab disease and insights into the response of tolerant pear plants to V. nashicola.

• 핵의학기술
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• 제21권2호
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• pp.55-64
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• 2017

최근 SPECT/CT의 보급과 함께 다양한 영상보정 방법들을 빠르고 정확하게 적용할 수 있게 되면서, 영상품질 향상과 더불어 정량적 정확성까지 기대할 수 있게 되었다. 그중 Collimator Detector Response (CDR) 회복(recovery)은 검출기면의 거리로부터 발생된 blurring 효과를 보상하여 분해능 회복을 목적으로 하는 보정방법이다. 본 연구에서는 SPECT/CT 영상에서 CDR recovery 가 적용되었을 때 검출거리 변화에 따른 정량적 변화를 알아보고자 하였다. 검출거리의 변화에 따른 획득 계수의 차이를 알아보고자 검출거리를 궤도방식(obit type)에 따라 Circular는 X, Y축 반경 30 cm, Non-Circular는 X, Y축 반경 21 cm, 10 cm, Non-Circular Auto(=Auto Body Contouring_ ABC, spacing limit 1 cm)로 설정하였고, 재구성 방법은 CDR recovery(CDRr)의 사용 유/무에 따른 계수 회복 차이를 알아보고자 OSEM (w/o CDRr)와 Astonish(3D-OSEM with CDRr)로 구분하여 적용하였다. 이 때 감쇠, 산란, 붕괴 보정은 모든 영상에 공통 적용하였다. 정량적 평가를 위해 교정인자(calibration factor_CF) 산출을 목적으로 교정영상(cylindrical phantom, $^{99m}TcO_4$ 123.3 MBq, 물 9293 ml)을 획득하였고, 팬텀 실험을 위하여 50 cc 주사기에 물 31 ml를 채우고 $^{99m}TcO_4$ 123.3 MBq를 설정하여 팬텀영상을 획득하였다. 팬텀 영상에서 주사기 전체 체적에 VOI(volume of interest)를 설정하여 각 조건별로 총 계수 값을 측정하였고, CF를 적용시켜 설정된 참값 대비 추정값의 오차를 구하여 보정에 따른 정량적 정확성을 확인하였다. 산출된 CF는 154.27 (Bq/ml/cps/ml)이며, 각 조건별 영상에서 참값 대비 추정값은 OSEM에서 Circular 86.5%, Non-Circular 90.1%, ABC 91.3% Astonish에서 Circular 93.6%, Non-Circular 93.6%, ABC 93.9%으로 분석되었다. OSEM은 검출거리가 가까울수록 정확성이 높아졌으며, Astonish의 경우에는 거리와 상관없이 거의 유사한 값을 나타내었다. 오차는 OSEM Circular(-13.5%)에서 가장 크고, Astonish ABC(-6.1%)에서 가장 적었다. SPECT/CT영상에서 CDR recovery 적용을 통한 거리보상이 이루어 졌을 때 검출거리가 먼 조건에서도 근접검출과 거의 동일한 정량적 정확성을 보였고, 검출거리의 변화에 영향을 받지 않고 정확한 보정이 가능한 것을 확인 할 수 있었다.

• 한국전산구조공학회논문집
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• 제28권1호
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• pp.71-78
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• 2015

LNG 선박에서 발생하는 슬로싱 충격하중은 다상유동 및 기체의 압축효과에 따라 CCS에서 발생하는 압력과 구조응답에 큰 영향을 미칠 수 있다. 본 연구에서는 슬로싱 운동 시 LNG의 유동에 의해 발생하는 슬로싱 충격을 시뮬레이션하기 위해서 다상유동을 적용한 수치해석 모델을 제시하였으며, 그 결과를 실험과 비교하여 타당성을 검토하였다. 또한 효율적인 구조 응답 계산을 위해 분사모델을 이용한 유체구조 연성해석 방법에 대해서 검토하고 멤브레인형 Mark III 화물창의 강도평가에 적용하여 LNG 화물창의 강도평가를 위한 가능성을 검토하였다.

• 대한후두음성언어의학회지
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• 제20권2호
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• pp.136-140
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• 2009

Background and Objectives: Recurrent respiratory papillomatosis (RRP) is difficult to treat because of its tendency to recur and spread throughout the aerodigestive tract. We aimed to estimate the effect of intralesional injections of cidofovir in patients with RRP. Materials and Method: Within the period from January 2003 to July 2007, 13 patients aged 2 to 61 years were treated with intralesional injections of cidofovir combined with surgical excision of RRP. Cidofovir was injected intralesionaly at a concentration of 5 mg/cc after complete removal of the papilloma with $CO_2$ laser or microdebrider. We evaluated the effect of intralesional cidofovir therapy by comparing pre-treatment mean interval of recurrence with post-treatment interval of recurrence. Results: Of 13 patients, two patients showed complete response during follow up period and four patients showed partial response. Seven patients did not respond to cidofovir at all. Mean pre-treatment mean interval of recurrence was 9 months and mean post-treatment interval of recurrence was 13.1 months (p=0.039). There was a statistical significance between the injected dose of cidofovir and post-treatment interval of recurrence (p=0.009). There were no local or systemic side effects caused by cidofovir. Conculsion : Intralesional injection of cidofovir seems to have a potential of a safe and effective adjuvant therapy of RRP. There was a positive correlation between the injected dose of cidofovir and patient clinical outcomes so that administration of higher doses and more frequency of injections should be needed to reduce recurrence. Further study regarding injection therapy regimen for RRP is required.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2009-2014
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• 2007

Hydrogen is a fuel of fuel cell system, which has powerful explosion possibility. Hence, the fuel cell system needs safety evaluation to prevent risk of hydrogen leakage. We use a actual size chamber of a common fuel cell module to analyze hydrogen. Hydrogen injection holes are located in lower part of the chamber in order to simulated hydrogen leakage. The hydrogen sensor can detect range of 0${\sim}$4%. Since the hydrogen gas, of which leaked amount is controled by MFC, are injected at the bottom holes, the transient sensor signals are measured. At a condition of 10cc/s of hydrogen leakage, the sensor detects hydrogen leakage after 22sec and there is also several seconds of time delay depending on the position of the sensor. This experimental data can be applied for the design of the hydrogen detection system and ventilation system of a residential fuel cell system.

• 대한의생명과학회지
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• 제17권3호
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• pp.217-223
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• 2011

${\beta}$-sitosterol glucoside exists in a variety of plants and have anti-tumor, anti-microbial, and immunomodulatory activities. Mast cells and eosinophils play important roles in a variety of inflammatory diseases, specifically asthma and atopic dermatitis. In the present study, we used lactose-${\beta}$-sitosterol (L-BS) and investigated the effect of L-BS on inflammatory responses of the human mast cell line, HMC-1 and the human eosinophilic leukemia cell line, EoL-1. In HMC-1 cells, L-BS significantly inhibited cell migration in response to stem cell factor without cytotoxicity. However, the mRNA expression of CC chemokine receptors (CCRs), including CCR1-5, were not altered after L-BS treatment in HMC-1 cells. LPS-induced IL-4 production was also suppressed by L-BS in a dose-dependent manner. In EoL-1 cells, the concentration ranging from 0.1 ${\mu}M$ to 10 ${\mu}M$ of L-BS had no cytotoxicity and had no effect on mRNA expression of major protein-mediators derived from activated eosinophils. However, 100 ${\mu}M$ of L-BS induced the apoptosis of EoL-1 cells in a time-dependent manner. This finding indicates the possibility of L-BS as a potential therapeutic molecule in inflammatory diseases and may contribute to the need to improve current therapeutic drugs.

• Ocean and Polar Research
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• 제35권2호
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• pp.127-134
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• 2013

In this study we investigated changes in the Tsushima Warm Current (TWC) under the global warming scenario RCP 4.5 by analysing the results from the World Climate Research Program's (WCRP) Coupled Model Intercomparison Project Phase 5 (CMIP5). Among the four models that had been employed to analyse the Tsushima Warm Current during the 20th Century, in the CSIRO-Mk3.6.0 and HadGEM2-CC models the transports of the Tsushima Warm Current were 2.8 Sv and 2.1 Sv, respectively, and comparable to observed transport, which is between 2.4 and 2.77 Sv. In the other two models the transports were much greater or smaller than the observed estimates. Using the two models that properly reproduced the transport of the Tsushima Warm Current we investigated the response of the current under the global warming scenario. In both models the volume transports and the temperature were greater in the future climate scenario. Warm advection into the East Sea was intensified to raise the temperature and consequently the heat loss to the air.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제54권2호
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• pp.69-75
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• 2005

In order to improve the reliability of high voltage rotating machines and mold transformers, it is necessary to understand the breakdown mechanism and life assessment of the high voltage winding parts. Especially the on-line PD test provides the ability to monitor effects, such as slot discharge, internal discharge, and end-winding discharge without interrupting the electrical machines, this method has been proven the major testing technology. Capacitive couplers have been the most widely used sensors for the on-line partial discharge detection in rotating machines nowadays. This paper deals with the electrical characteristics and long-term reliability of a ceramic coupler(CC), which can be easily mounted into high voltage input terminal part, has been developed and tested to continuously measure PD activity during operating condition. This paper presents electrical characteristics (dielectric loss angle, capacitance, PD inception level, breakdown voltage, and frequency response bandwidth) and long-term life test result of the developed 6.6 kV class on-line ceramic coupling sensor. It was found that this sensor had good electrical characteristics to detect PD activity during the operating condition with its detection frequency band is between several and several tens MHz. Also, the voltage life of the 6.6kV class ceramic coupler was calculated over 60 years.

• 한국시뮬레이션학회논문지
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• 제23권4호
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• pp.189-195
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• 2014

본 논문에서는 장소와 시간의 제약을 받지 않는 클라우드 컴퓨팅 도입 의사 결정 모델링에 대한 연구를 진행하였다. 연구에서는 65명의 응답자에게 수집 된 패널데이터와 데이터마이닝 방법 중 하나인 CART(회귀분류나무)를 사용하여 의사결정 모델을 구축하였다. 모델링에는 2단계로 진행되는데 첫 번째 단계에서는 패널데이터를 사용하여 도입 의사를 결정하는데 영향을 미치는 문항들을 선택하고 2 번째 단계에서는 선택된 문항을 사용하여 도입 의사 결정 모델을 구축하였다. 문항 선택을 통하여 설문지 수집 문항수를 25개에서 5개로 줄일 수 있어 응답자에게 빠른 답변을 얻을 수 있고 데이터의 사이즈가 작기 때문에 모델 구축 시간을 줄일 수 있는 장점을 보여주었다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2012년도 추계학술대회 논문집
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• pp.344-349
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• 2012

This paper presents a new small-sized damper featuring magneto-rheological (MR) fluid which can be applied to vibration control system. The proposed MR damper consists of cylinder, piston, a couple of bearings, oil-seals and magnetic circuit which has two coils. In this damper, approximately 5cc of MR fluid is used. The damping force of the MR damper is designed to be followed by linear shear-mode Bingham-plastic model. In order to verify the performance of the MR damper, an experimental apparatus is established. In the experimental test, the damping force of the MR damper is measured with respect to time, displacement and velocity. In addition, the time response of MR damper is measured when 1A of step current is applied. Finally, The proposed small MR damper is applied to vibration control. In this process, a simple 1-DOF system is modeled and controlled using PID controller.