• Title/Summary/Keyword: CAE

Search Result 1,150, Processing Time 0.031 seconds

Antibacterial and Whitening Activities of Coffea arabica Ethanol Extract (커피 에탄올 추출물의 항균 및 미백활성)

  • Kim, In Hae;Lee, Jae Hwa
    • Korean Chemical Engineering Research
    • /
    • v.56 no.2
    • /
    • pp.245-251
    • /
    • 2018

  • In this study, Coffea arabica ethanol extract (CAE) was tested for possible functional cosmetic agent. Whitening effect was measured by tyrosinase inhibition assay, and anti-oxidant activity was checked by SOD-like activity. SOD-like activity of CAE showed $94.8{\pm}6.2%$ at $500{\mu}g/mL$. The anti-bacterial activities CAE was evaluated against three different gram-positive bacteria and six gram-negative bacteria including MRSA strains. CAE exhibited in vitro broad spectrum antimicrobial activities of gram-negative bacteria without antifungal activity. CAE was strong exhibited against MRSA CCARM3561. The tyrosinase and L-DOPA inhibitory activities of the CAE lower than those positive control arbutin. CAE reduced melanin contents of B16-F10 melanoma cell in a dose dependent manner and decrease about 89.2% at a concentration $100{\mu}g/mL$. These result highlight the potential of coffee extract as a naturally active and non-toxic antibacterial suitable for cosmetic applications.

  • https://doi.org/10.9713/kcer.2018.56.2.245 인용 PDF KSCI

Intestinal Immunomodulatory Effect and Marker Compound of Centella asiatica Extracts-Added Beverage Prototype (병풀 추출물이 첨가된 음료 시제품의 장내 면역조절 효과와 지표물질)

  • Yeon Suk Kim;Hyun Young Shin;Ja Pyeong Koo;Eun Ji Ha;Won Bi Jeong;Mi Yeun Joung;Kwang-Won Yu
    • The Korean Journal of Food And Nutrition
    • /
    • v.36 no.6
    • /
    • pp.436-444
    • /
    • 2023

  • To produce an intestinal immunomodulatory beverage containing Centella asiatica extract (CAE), three types of CAE-added beverage prototypes were prepared, and their immunomodulatory activities and marker compounds were analyzed. As a result of the cytotoxicity assessment, all the beverages did not show significant toxicity compared to the control group. Next, the immunomodulatory activities of the beverage prototype were evaluated using the inflammatory model of IL-1β-induced intestinal epithelial cell line. All the samples significantly reduced the production of IL-6, IL-8, and MCP-1 in a CAE concentration-dependent manner. In addition, CAE-added beverages inhibited NO, IL-6, and IL-12 production in LPS-induced RAW 264.7 cells. When the major triterpenoids, as marker compounds for the production of CAE-added beverages, were analyzed by HPLC-DAD, only asiaticoside was detected beyond the limit of quantification, while madecassoside, madecassic acid, and asiatic acid were not detected. The amounts of asiaticoside in CAE-added beverage prototypes were confirmed in No. 1 (19.39 ㎍/mL), 2 (19.25 ㎍/mL), and 3 (19.98 ㎍/mL). In conclusion, the results of this study suggested that CAE-added beverage prototypes induced immunomodulatory effects in the intestinal inflammatory cell line models and asiaticoside could be used as a marker compound for CAE-added beverage production.

  • https://doi.org/10.9799/ksfan.2023.36.6.436 인용 PDF