• Journal of Embryo Transfer
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• v.22 no.3
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• pp.149-154
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• 2007

Endometrial cells play important roles in implantation and during pregnancy. This study was carried out to identify whether two-pore domain $K^+\;(K_{2P})$ channels are expressed in endometrial cells of Korean cattle. $K_{2P}$ channels set the resting membrane potential of many kinds of neuronal cells in the central and peripheral nervous systems. RT-PCR data showed that TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were expressed in bovine endometrial cells, and the mRNA expression levels were similar between endometrial cells with or without endometritis. The protein expression was confirmed by using commercially available polyclonal antibodies (TASK-3, TREK-1, TREK-2, and TRAAK). TASK-3 and TREK-1 were expressed in all area of endometrial cells including nuclei, while TREK-2 and TRAAK were expressed in all area of cells except nuclei. These results demonstrate for the first time the presence of $K_{2P}$ channel in endometrial cells of Korean cattle.

• Journal of Society of Preventive Korean Medicine
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• v.20 no.3
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• pp.55-65
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• 2016

Objectives : Mibyeong is a korea medicine have original concept of the disease. However, no previous report has effect of mibyeong herbal medicine in fatigue types of mibyeong. This study investigated the question of whether Dang Gui Bo Hyul-tang(DGBHT) of effect on fatigue types of Mibyeong. Methods : C57BL/6 mice were randomaly divided into three group (n=10). The mice were then group (1) Nocontrol, (2) Restraunt stress(veh), (3) Dang Gui Bo Hyul-tang 200mg/kg. The administered Dang Gui Bo Hyul-tang 200mg/kg or distilled water (orally) 1 hr prior to daily exposure to repeated restraint stress (2 h) for 15 days. The performed behavior test (Mechanical hyperalgesia test,, Open-field test, Forced swimming test, Sucrose preference test and immunostaining and biochemical measured in serum. Results : Stress fatigue induced mices significantly increased lethargic, hyperalgesia through behavior test (Mechanical hyperalgesia test (decrease 43%), Open-field test ($4,809{\pm} 226.13cm$ vs. $3121{\pm}226.64cm$), Forced swimming test ($11.45{\pm}3.96$ vs. $79.10{\pm}8.12sec$), Sucrose preference test (decrease 58%)). In addition, chronic fatigue model evidently increased corticosterone level ($122.54{\pm}18.88$ vs. $186.94{\pm}18.26ng/ml$), AST level ($46.22{\pm}3.23$ vs. $31.40{\pm}3.86U/L$), ALT level ($38.78{\pm}5.72$ vs. $17.60{\pm}1.30$), liver necrosis, lateral ventricle size. These alterations were significantly ameliorated by DGBHT. DGBHT significantly attend the elevated serum concentrations of corticosterone ($155.90{\pm}6.29ng/ml$), AST ($31.40{\pm}3.86U/L$), ALT ($17.60{\pm}1.30U/L$). Moreover, DGBHT improved lethargic, hyperalgesia when compared the stress fatigue (Mechanical hyperalgesia test (improve 28%), Open-field test ($4,038{\pm}615.81cm$), Forced swimming test ($7.56{\pm}1.88sec$), Sucrose preference test (increase 21%) Conclusions : Theses result suggest that DGBHT have improved lethargic, hyperalgesia and fatigue-associated hormone and liver protective on stress fatigue model. It will be necessary to research to present evidences on benefits and effects of Korean medical treatment for Mibyeong through clinical researches based on benefits and effects of those animal models.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.1
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• pp.37-43
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• 2010

The serine/threonine kinase Akt has been shown to play a role of multiple cellular signaling pathways and act as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI3K). It has been reported that phosphorylated Akt activates eNDS resulting in the production of NO and that NO stimulates soluble guanylate cyclase (sGC), which results in accumulation of cGMP and subsequent activation of the protein kinase G (PKG). It has been also reported that PKG activates PI3K/Akt signaling. Therefore, it is possible that PI3K, Akt, eNOS, sGC, and PKG form a loop to exert enhanced and sustained activation of Akt. However, the existence of this loop in eNOS-expressing cells, such as endothelial cells or astrocytes, has not been reported. Thus, we examined a possibility that Akt phosphorylation might be enhanced via eNOS/sGC/PKG/PI3K pathway in astrocytes in vivo and in vitro. Phosphorylation of Akt was detected in astrocytes after KA treatment and was maintained up to 72 h in mouse hippocampus. 2 weeks after KA treatment, astrocytic Akt phosphorylation was normalized to control. The inhibition of eNOS, sGC, and PKG significantly decreased Akt and eNDS phosphorylation induced by KA in astrocytes. In contrast, the decreased phosphorylation of Akt and eNDS by eNDS inhibition was significantly reversed with PKG activation. The above findings in mouse hippocampus were also observed in primary astrocytes. These data suggest that Akt/eNOS/sGC/PKG/PI3K pathway may constitute a loop, resulting in enhanced and sustained Akt activation in astrocytes.

• Progress in Medical Physics
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• v.21 no.1
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• pp.93-98
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• 2010

The aim of this work was to establish the methodology for event positioning by measuring depth of interaction (DOI) information and to evaluate the system sensitivity and spatial resolution of the new detector for I-125 and Tc-99m imaging. For this purpose, a Monte Carlo simulation tool, DETECT2000 and GATE were used to model the energy deposition and light distribution in the detector and to validate this approach. Our proposed detector module consists of a monolithic CsI(Tl) crystal with dimensions of $50.0{\times}50.0{\times}3.0\;mm^3$. The results of simulation demonstrated that the resolution is less than 1.5 mm for both I-125 and Tc-99m. The main advantage of the proposed detector module is that by using 3 mm thick CsI(Tl) with maximum-likelihood position-estimation (MLPE) method, high resolution I-125 imaging and high sensitivity Tc-99m imaging are possible. In this paper, we proved that our new detector to be a reliable design as a detector for a multi-energy SPECT.

• Korean Journal of Food Science and Technology
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• v.10 no.2
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• pp.189-193
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• 1978

Effects of the special media on the mycelium growth in Agaricus campestris has been studied. The results are summarized as follows; 1. The mycelium growth of Agaricus campestris were considerably Stimulated on the Carrot (Dancus carota L.) basal medium which was added 4ml. of carrot extract, Cucumber(Cucumis sativus L.) basal medium added 3ml of cucumber extract, and Radish (Rhaphanus sativus L.) basal medium added 3ml. of radish extract during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts shows a lot of growth for 144 hours. The orders are as follows; Carrot basal medium(4ml/100ml)>Cucumber basal medium (3ml/100ml)>Radish basal medium (3ml/100ml)>Lettuce basal medium (2ml/100ml)>Cabbage basal medium (2ml/100ml). However, the Lettuce (Lactuca scariota L.) basal medium and the Cabbage (Brassica chinensis L.) basal medium among these five media are no significant differences.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.2
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• pp.169-182
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• 2007

To evaluate the possibly potent role of Asian Dust (AD) on the long range transport of Hg, statistical analyses were carried out using the hourly concentration data of gaseous elemental mercury (Hg) along with relevant environmental parameters. For the purpose of this study, Hg data were collected from Yang-Jae monitoring station in Seoul, Korea during Sept. 1997 to June 2002. During the study period, Hg concentrations in non-AD period ranged from $0.03\;to\;32.70\;ng\;m^{-3}$ with a mean $5.27{\pm}3.06\;ng\;m^{-3}$, while those in AD period from $1.79\;to\;32.60\;ng\;m^{-3}$ with a mean $5.20{\pm}3.06\;ng\;m^{-3}$. The air quality during AD were typically deteriorated by enhanced PM10 mass concentration (by $2{\sim}5$ times) compared to non-AD period, however comparison of the Hg concentration data indicates that they are not critically distinguished between events of AD and non-AD, except for the high minimum level of Hg during AD. The results of correlation and factor analysis also indicated somewhat complex patterns; in the case of AD events, Hg and $SO_{2}$ were assorted concurrently into a same factor. Evidence collected from this study thus suggests that long-range transport of Hg, if occurring, is unlikely to raise statistically Hg concentration levels such as seen during AD event. However, in nighttime of winter season, Hg concentrations are higher during AD (along with PM10 levels) than non-AD period. Although such observations suggest the effect of long range transport on the enhancement of Hg concentrations, more deliberate analysis may be required to track down the effect of such mechanism in relation with various factors including the air mass transport route.

• Journal of Nutrition and Health
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• v.13 no.4
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• pp.187-194
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• 1980

To estimate the amount of daily salt intake by source of food and urinary sodium excretion, a dietary survey was conducted from August 27th to September 3, 1980. The salt concentration of six kinds of food was measured. The families of 25 womens' club leaders and 25 families of hypertensive patients were studied after they had been given a short course of education on lowsalt diet. The results were as follows: 1. The average amount of high-salt foods eaten daily by the study group were as follows: 4.5 Gm. in the form of table salt 16.8 ml of home-made soy sauce 6.20 ml of commercial soy sauce 11.9 Gm. of home-made red-pepper bean paste 12.0 Gm. of home-made soy-bean paste 120.7 Gm. of Kimchi 2. The average NaCl concentration of each food was as follows: 99.5% in table salt, 22.5% in home made soy sauce, 11.9% in Commercial soy sauce, 6.8 Gm% in home made red pepper bean paste, 9.2 Gm% in home-made soy-bean paste and the average concentration of various kinds of Kimchi was 2.7 Gm%. 3. The total amount of daily salt intake per adult was calculated as 14.3 Gm. By sources of food 4.5 Gm from table salt, 3.9 Gm from home-made soy sauce and 3. 3 Gm from Kimchi were taken daily Three other kinds of food were also minor sources of salt intake.4. The average amounts of individual daily salt intake were not significantly different between the hypertensive group and the normotensive group: the median of the normotensive group (11.7Gm.) was significantly lower than that of the hypertensive group (14.9Gm.). Therefore the womens' club leaders appeared to respond more quickly than the hypertensive group after low-salt diet education. 5. The average amount of sodium excretion in the 24 hour urine specimen was 234.7 mEq. From this finding the daily NaCl intake was estimated to be 15.8Gm.

• Journal of the Korea Concrete Institute
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• v.18 no.5 s.95
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• pp.621-630
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• 2006

Twelve beams made of lightweight high-strength concrete were tested to determine their diagonal cracking and ultimate shear capacities. A total of 12 beams without(4 beams) and with lightweight(8 beams) were tested in a stiff testing facility, and complete load-midspan deflection curves, including the maximum capacities portion, were obtained. The variables in the test program were concrete strength, which varied 35.4 MPa, 65.3 MPa; shear span-depth ratios a/d=1.5, 2.5, 3.5, 4.5; and tensile steel ratio between 0.57 and 2.3 percent. Also, we divided beam by diagonal tension crack and ultimate shearing strength to propose an equation. In addition, it analyzed comparison mutually applying existing proposal and guide. $V_{cr}$ was as result that AIK recommendations and Zsutty proposal decrease more than a/d=2.5, increased some in Mathey's proposal equation. $V_{cr,\exp}/V_{cr,cal}$ showed tendency of overestimation according to increase of tensile steel ratio and compressive strength of concrete. On the other hand, $V_{cr,\exp}/V_{cr,cal}$ is superior in conformability with an experiment result Zsutty's proposal among other equations. The proposal equation hew that expect $V_{cr}/V_u$, rationally about shearing strength. Therefore, shear strength an equation is considered to be utilized usefully evaluating capacity by change of the shear span depth ratio of lightweight concrete, tensile steel ratio, and compressive strength of the concrete in this research.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.12
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• pp.1637-1642
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• 2007

The objectives of this study were to compare the aroma characteristics and antioxidant activity of raw and heated garlic ($130^{\circ}C$, 2 hr) from different cultivation areas (Danyang, Seosan, Uiseong Namhae, Namdo, Daeseo and China). The volatile compounds were extracted by simultaneous steam distillation extraction and identified with gas chromatography/mass spectrometer. The major volatile compounds of raw garlic were sulfur compounds such as diallyl disulfide, methyl-2-propenyl trisulfide, di-2-propenyl trisulfide etc. After heating, the major volatile compounds were allyl mercaptan, methyl pyrazine, 2,2-dimethyl-1,3-dithiane, 2-propenyl propyl disulfide, allyl methyl sulfide, allyl alcohol, and allyl sulfide etc. The DPPH radical scavenging activity (EDA, %) of volatile compounds from raw garlic and heated garlic was increased in a dose-dependent manner. The antioxidant activities (EDA, %) of volatile compounds from raw garlic cultivated in Danyang, Namhae and China were 20.07, 34.62, and 9.71% respectively. After heating, these values were increased to 79.90, 93.59, and 77.26% respectively. Results showed that heat treatment significantly enhanced the antioxidant activities (EDA, %) of the garlic.

• The Korean Journal of Food And Nutrition
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• v.29 no.4
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• pp.547-556
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• 2016

The purpose of this study was to investigate the relationship between frequency of coffee consumption, metabolic biomarkers, and nutrition intake in adult participants in the combined 2007~2009 Korean National Health and Nutrition Examination Survey (KNHANES). Subjects (2,095 males and 3,297 females) were classified according to sex and frequency of coffee consumption (${\leq}1$ time/month, ${\geq}2$ times/month and ${\leq}6$ times/week, 1 time/day, 2 times/day, 3 times/day) using food frequency questionnaires. Nutrition intake was analyzed using 24 h recall data. The 3 times/day coffee consumption group had a significantly higher age, and frequency of smokers and drinkers compared to the ${\leq}1$ time/month coffee consumption group in both male and female participants. Males in the 3 times/day coffee consumption group had a significantly lower HDL-cholesterol level, but females had a higher waist circumference compared with the ${\leq}1$ time/month coffee consumption group. Males in the 3 times/day coffee consumption group had a significantly lower nutrient density of fiber, vitamin B2, vitamin C, calcium and phosphorus compared with the ${\leq}1$ time/month coffee intake group. Females in the 3 times/day coffee consumption group had a significantly higher nutrient density of fat and niacin, but lower nutrient density of carbohydrate, calcium, phosphorus, and iron compared with the ${\leq}1$ time/month coffee intake group. In males, the frequency of coffee consumption was not associated with the levels of metabolic biomarkers. In females, the frequency of coffee consumption was positively associated with diastolic blood pressure after adjustments for multiple confounding factors, including age, BMI, smoking status, alcohol consumption, physical activity and energy intake. Coffee consumption was associated with decreased diastolic blood pressure in females. These findings suggest the importance of an awareness of the association between coffee consumption and metabolic risk.