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Expression of Two-pore Domain $K^+$ Channels in Endometrial Cells of Korean Cattle  

Kang, D. (Department of Physiology, College of Medicine Gyeongsang National University)
Kim, E.S. (Department of Physiology, College of Medicine Gyeongsang National University)
Yang, H.Y. (Department of Physiology, College of Medicine Gyeongsang National University)
Choe, C.Y. (Animal Genetic Resources Station, National Institute of Animal Science, RAD)
Han, J. (Department of Physiology, College of Medicine Gyeongsang National University)
Publication Information
Journal of Embryo Transfer / v.22, no.3, 2007 , pp. 149-154 More about this Journal
Abstract
Endometrial cells play important roles in implantation and during pregnancy. This study was carried out to identify whether two-pore domain $K^+\;(K_{2P})$ channels are expressed in endometrial cells of Korean cattle. $K_{2P}$ channels set the resting membrane potential of many kinds of neuronal cells in the central and peripheral nervous systems. RT-PCR data showed that TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were expressed in bovine endometrial cells, and the mRNA expression levels were similar between endometrial cells with or without endometritis. The protein expression was confirmed by using commercially available polyclonal antibodies (TASK-3, TREK-1, TREK-2, and TRAAK). TASK-3 and TREK-1 were expressed in all area of endometrial cells including nuclei, while TREK-2 and TRAAK were expressed in all area of cells except nuclei. These results demonstrate for the first time the presence of $K_{2P}$ channel in endometrial cells of Korean cattle.
Keywords
two-pore domain $K^+$ channel; endometrial cell; Korean cattle; endometrium;
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