• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.5
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• pp.472-479
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• 2012

The formation of volatile fatty acids(VFAs) and changes in pH, oxidation and reduction potential(Eh) and acid volatile sulfide(AVS) with the addition of yellow clay were investigated using microcosm systems to examine the effects of yellow clay dispersion on the anaerobic decomposition of Cochlodinium polykrikoides in marine sediments. The acetate concentration reached a maximum by day 4 and was 1.2-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (224-270 vs. 333 uM). The formate concentration reached a maximum by day 1 and was 1.3-2.8 fold less in the sample treated with yellow clay compared to the untreated sample (202-439 vs. 563 uM). The propionate concentration reached a maximum by day 2 and was 1.5-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (32.6 vs. 57.2 uM). After the amounts of acetate, formate and propionate peaked the levels dropped dramatically due to the utilization by sulfate reducing bacteria. The Eh of the samples treated with yellow clay was similar to the untreated sample on day 0 but was higher in the sample treated with yellow clay(140-206 mV) from days 4 to 17. AVS started to form on day 3 and this was sustained until day 6, and 1.2-2.2 fold less was produced in the sample treated with yellow clay compared to the untreated sample (40.2-69.3 vs. 83.2-93.8 mg/L). Accordingly, during the anaerobic decomposition of C. polykrikoides in marine sediments, yellow clay dispersal seems to suppress the reduction state of Eh and the formation of volatile fatty acids(acetate, formate and propionate) used as an energy source by sulfate reducing bacteria, indicating that this process controls the production of hydrogen sulfide that negatively affects marine organisms and the marine sediment environment.

• Journal of the Mineralogical Society of Korea
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• v.19 no.4 s.50
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• pp.277-289
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• 2006

This study was carried out to find a new material having high removal efficiency for the harmful red tide. C. polykrikoides grow very fast and accumulate into dense and visible patches near the surface of the seawater ('Water bloom'). Some mineral medicines and Hwangto (reddish soil consist of clay minerals and Fe-oxides) were used in this study to remove C. polykrikoides. The pre-determined sprinkling ratio of mineral vs. seawater which contains approximately 5,000 cells/mL of C. polykrikoides was 10 g/L. In order to quantify the removal efficiency, the density of living cells was measured by counting with the Intervals of 0, 10, 30, and 60 minutes after sprinkling. Five Hwangtos feom different localities were examined in this study. It is found that a material with a high concentration of Fe and Al was the most effective to remove C. polykrikoides. After the sprinkling of the Hwangto showing the best removal efficiency in the test, 99% of total algaes were found to be eliminated within 60 minutes. Jeokeokji showed the highest removal efficiency among clay mineral medicines(92% removal efficiency after 60 minutes), and the rests in decreasing order are as follows: Gamto (91%) > Baekseokji (89%) > Hydromica (81%). In addition, Fe-oxide mineral medicine similarly looking as fine-grained earthy Daejaseok showed 100% removal efficiencyafter 30 minutes, and Wooyoeryang, 95% after 60 minutes. It is noted that even little addition (1 g/L) of Daejaseok, 10% of Hwangto concentration into seawater showed the removal efficiency of 100% after 60 minutes. From the results, it could be concluded that the fine-grained earthy Daejaseok was the most effective natural mineral medicine to remove the C. polykrikoides from seawater. Under the microscope the removal mechanism was found to be activated in the following order: adsorption, swelling of chain colony, chain colony crisis and algaecide.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.4
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• pp.370-379
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• 2007

Short-term variations of phytoplankton community structure in the southeast coastal waters of Korea from July to September in 2006 were investigated with data set of phytoplankton, chemical and physical water properties, and meterological data. A total of 11 sampling sites of 4 different depths (surface, 5 m, 10 m, and bottom) were visited on July 11-14, July 24-26, August 7-10, August 21-24 and September 5-8. We identified 151 species in 63 genera of phytoplankton in which diatoms were the most diverse group composed of 92 species in 37 genera. Dinoflagellates were the second diverse group of 52 species in 22 genera. The other groups include 7 species in 4 genera including Raphidophytes, and Euglenophyta. After rainy season, excessive nutrients from adjacent streams to the stratified water column proliferates Chaetoceros group in July. But biomass of phytoplankton and nutrient concentrations were decreased during the period of a drought in August. However, Chaetoceros was the most dominant genera in all depths of the first, second, third and fifth cruises, except the 4th cruise on August 21-24 when dominant group were dinoflagellates including Gymnodinium spp. and Cochlodinium polykrikoides. The characteristic of phytoplankton community and environment condition during summer 2006 can be summarized as: 1) low concentration of nutrients caused by a long lasting drought in August 2) no summer outbreak of C. polykrikoides because the strength of offshore waters was weak than other years, and 3) Chaetoceros spp. was the dominant species despite short period appearance of dinoflagellates.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.225-230
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• 2005

The aim of this study was to examine the fluctuation in phytoplankton assemblages with regarding to environmental conditions and nutrients, which were surveyed quarterly over the fours seasons (February, May, July, and October). In turn, an understanding of biological effects should provide insights into a wide range of initiated Cochlodinium blooms in Narodo. Sampling was carried out throughout 2001 on the coasts of Busan (St. 1), Yeosu (St. 2), Narodo (St. 3), Kohung (St. 4), and Kwangdo (St. 5). The maximum surface water temperature was recorded in July, and it ranged from 20 to $22^{\circ}C$. Salinity showed no great variation, which maintained itself in the range of 29-34 psu. The maximum surface salinity was recorded in February, which was about 34 psu. The chlorophyll $\alpha$ concentration of the surface water ranged from 0.01 to $1.3\;{\mu}g\;1^{-1}$. The concentrations of $NH_{4}-N $ were persistently high from February to October; in particular, the peak was observed at St. 1 in February and May (0.15 and $0.19\;{\mu}mol\;1^{-1}$, respectively), while it was detected at St. 2 in July and October (0.22 and $2.2\;{\mu}mol\;1^{-1}$ respectively). Similar trends to those for $NH_4-N $ were observed in the concentrations of $NO_{2}-N$ and $NO_{3}-N$. In contrast to nitrogen, a distinct peak of $NO_{4}-P$ at St. 3, 4, and 5 was observed throughout year $(0.01-0.1\;{\mu}mol\;1^{-1}$ except for October. At St. 1 encounter a peak of cell number of 30,000 and $13{\times}10^3$ cells $1^{-1}$, respectively, in July and October. During the period of this study, the majority of the taxa were diatoms. The dinoflagellates were rather abundant after February, in particular at St. 3, 4, and 5 which attained an abundance of $10\~20\%$without marked fluctuation during the period of this study. At St. 3, the highest average cell width, $178.11\;{\mu}m$, was recorded: the highest cell length, $337.72\;{\mu}m$, was measured in July. Consequently, dinoflagellates bloom in July at Narodo influenced by warm water current are not only associated with a desirable development of cell morphometric characteristics, but also with the health growth of C. polykrikoides. During the period of this study, warm water currents caused an increased water temperature in Narodo, but did not change the amount of nutrients.

• Korean Journal of Remote Sensing
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• v.25 no.6
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• pp.475-486
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• 2009

Harmful algal blooms (HAB) caused by the dominant species Cochlodinium polykrikoides (C. polykrikoides) appear in the South Sea of Korea and are particularly present in summer and fall seasons. Environmental factors such as water temperature, weather conditions (air temperature, cloud cover, sunshine, precipitation and wind) influence on the initiation and subsequent development of HAB. The purpose of this research was to study spatial and temporal variations of HAB in the Yeosu area using environmental (oceanic and meteorological) and satellite data. Chlorophyll-a concentrations were calculated using Sea-viewing Wide Field-of-view Sensor (SeaWiFS) images by an Ocean Chlorophyll 4 (OC4) algorithm, and HAB were estimated using the Red tide index Chlorophyll Algorithm (RCA). We also used the surface velocity of sequential satellite images applying the Maximum Cross Correlation method to detect chlorophyll-a movement. The results showed that the water temperature during HAB occurrences in August 2002-2008 was $19.4-30.2^{\circ}C$. In terms of the frequency of the mean of cell density of C. polykrikoides, the cell density of the HAB found at low (<300 cells/ml), medium (300-1000 cells/ml), and high (>1000 cells/ml) levels were 27.01%, 37.44%, and 35.55%, respectively. Meteorological data for 2002-2008 showed that the mean air temperature, precipitation, wind speed and direction, and sunshine duration were $22.39^{\circ}C$, 6.54 mm/day, 3.98 m/s (southwesterly), and 1-11.7 h, respectively. Our results suggest that HAB events in the Yeosu area can be triggered and extended by heavy precipitation and massive movement of HAB from the East China Sea. Satellite images data from July to October 2002-2006 showed that the OC4 algorithm generally estimated high chlorophyll-a concentration ($2-20\;mg/m^3$) throughout the coastal area, whereas the RCA estimated concentrations at $2-10\;mg/m^3$. The surface velocity of chlorophyll-a movement from sequential satellite images revealed the same patterns in the direction of the Tsushima Warm Current.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2005.05a
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• pp.241-244
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• 2005

The aim of this study was to determine the first outbreaks of nontoxic Gonyaulax polygramma Stein in Yeosu waters in place of harmful Cochlodinium polykrikoides Margalef, which has occurred annually in the same coastal region since 1995. The observation of cellular arrangement and structure by electron microscopy showed that G. polygramma isolated from Yeosu waters had a few spines connecting with mem-branes and prominent longitudinal ridges on the cell surface, with a cingular dis-placement 1.5 times their cell width. Furthermore, the location of the nucleus wasposterior of large oval formation according to electron microscopy. On 6 August, 2004,the first bloom of G. pozygramma occurred, the date of own its disappearance was with a maximum cell density of 8,000 cells ml$^{-1}$ on 21 August, 2004. During the period of this study, the horizontal distribution of sea water temperature and salinity showed a strong coastal front, whereas the front of DIN (Dissolved Inoganic Nitrogen) was significantly different between the occurrence and disappearance of G. polygramma blooms. These results suggested that the process of the breakdown of stratification by wind and a low level of inorganic nitrogen play important roles in the rapid growth of G. polygrmma, which is associated with a greater robustness in growth against DIN than that of C.polykrikoides in nature.

• Korean Journal of Ichthyology
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• v.30 no.4
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• pp.194-204
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• 2018

In order to minimize the damage on the red sea bream Pagrus major by a harmful dinoflagellate Cochlodinium polykrikoides, we investigated the effect of feeding, starvation and stocking density on the survival rate, growth, growth restoration and physiological response of P. major exposure to C. polykrikoides. The experimental groups were divided into three groups such as F-HD (feeding and high density with $6.4kg/m^3$), S-HD (starvation and high density with $6.4kg/m^3$) and S-LD (starvation and low density with $3.2kg/m^3$) according to stocking density and starvation in marine cage ($11m{\times}11m{\times}5m$). The F-HD was fed throughout the experiment for 9 weeks, whereas S-HD and S-LD were not fed for 5 weeks and then refeeding for 4 weeks. Survival rate was the lowest in F-HD (85.5%) and S-LD was the highest (97.3%). The growth rates of S-HD and S-LD were significantly lower than F-HD during starvation period for 4 weeks, but rapidly recovered after feeding. The nutritional status such as ALB, TP, TCH, TG were similar to tendency of growth data. Ht, Hb, AST, ALT and GLU levels were significantly higher in the F-HD than in the starvation groups at the same time (in 3 week) during starvation period. But starvation groups did not differ during starvation period. As a result, F-HD is more sensitive to stress than S-HD and S-LD. Thus, during C. polykrikoides bloom period, starvation and stocking density control can help survival and growth restoration of the red sea bream.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.452-457
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• 1999

Algicidal bacteria were isolated from the mud of south coastal sea of Korean peninsula and screened to evaluate algicidal activity on Cochlodinium polykrikoides. The optimum condition for the development of highest algicidal activity was determined, The optimum sodium chloride concentration for algicidal activity of isolated algicidal bacteria was $3\%$. The optimum temperature, pH and culture time for the highest algicidal activity were $30^{\circ}C$, pH 7 and above 24 hr, respectively. The algicidal activity were significantly increased at the stationary phase of the cell growth and continuously increased up to maximum at the death phase, probably due to the release of algicidal substance by cell Iysis. The effect of zinc ion addition on algicidal activity, was observed and indicated that the substance requires zinc for its activity. The candidate for the algicidal substance may be $\alpha$-mannosidase.

• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.205-215
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• 2009

In order to understand on the seasonal dynamics of phytoplankton in the mouth parts of Gamak Bay, Korea, we investigated from one station during June 2005 to June 2006. Samples were collected every week during the high water temperature (${\geq}20^{\circ}C$), and every two weeks during the low water temperature (${\leq}20^{\circ}C$). The phytoplankton community were composed of 76 genera, 208 species in the surface layer, and 72 genera, 186 species in the bottom layer (total: 77 genera, 214 species). The number of occurring species fluctuated greatly throughout the year from 27 species (March 15, 2006) to 121 species (Aug. 16, 2005). High number of species showed between June and September (>70 species), whereas the low number of species showed during the period of the low water temperature below $20^{\circ}C$ (ca. 40 species). The predominant species were diatom Skeletonema costatum during whole year, and two naked dinoflagellates, Cochlodinium polykrikoides and Polykrikos kofoidii during high temperature seasons. However, centric diatoms, Chaetoceros curvisetus, Chaetoceros debilis and Eucampia zodiacus were dominant species during low temperature seasons. Standing crops of dinoflagellate showed great fluctuations, which were ranged from $3.0{\times}10^5\;cells\;L^{-1}$ (April 17, 2006) to $7.3{\times}10^5\;cells\;L^{-1}$ (Aug. 2, 2005) in the surface layer and from $1.5{\times}10^3\;cells\;L^{-1}$ (Nov. 9, 2005) to $3.9{\times}10^5\;cells\;L^{-1}$ (Aug. 16, 2005) in the bottom layer, and showed high during high temperature seasons such as the number of species. The fluctuations were greater in the surface layer than in the bottom layer. Moreover, the variation of Cochlodinium polykrikoides, is affected by Polykrikos kofoidii grazing.

• Korean Journal of Environmental Biology
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• v.28 no.3
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• pp.115-124
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• 2010

In order to find out the short-term phytoplankton dynamics during summer season in Jinhae Bay, we investigated the environmental factors and phytoplankton population densities at 16 stations. A total of 66 phytoplankton species were appeared, diatoms occupied more than 80% of total species, and the others were dinoflagellates and raphidophyceaes. The dominant species were diatoms Chaetoceros affinis, C. didymus, C. pseudocurvisetus, Leptocylindrus danicus, Pseudonitzchia pungens, Skeletonema costatum and dinoflagellates Cochlodinium polykrikoides, Gymnodinium impudicum, Karenia mikimotoi, Prorocentrium minimum, Protoperidinium bipes and Scrippsiella trochoidea. According to multidimensional scaling (MSD) and cluster analysis based on phytoplankton community data from each station, the Jinhae bay was divided into two groups. The first group included stations in the inner part of Masan Bay, which is strongly influenced by high nitrogen loading and low salinity by river discharge. The second group included the other remained stations. Based on canonical correspondence analysis (CCA), small-size dinoflagellates K. mikimotoi, P. minimum, P. bipes and S. trochoidea and centric diatom C. pseudocurvisetus and S. costatum, which is dominanted in the inner area of bay, were related with DIN (ammonium, nitrate, nitrite). These results imply that the study area was grouped into the two water masses (inner and outer water of Masan Bay) and inner waters had higher phytoplankton abundances and Chl.a concentration than outer waters. As a result, summer phytoplankton peaks in the inner shallow bay were stimulated by increases in nitrogen and temperature.