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Ginsenosides from the fruits of Panax ginseng and their cytotoxic effects on human cancer cell lines (인삼(Panax ginseng) 열매로부터 분리한 ginsenoside의 동정 및 암세포독성 효과)

  • Gwag, Jung Eun;Lee, Yeong-Geun;Hwang-Bo, Jeon;Kim, Hyoung-Geun;Oh, Seon Min;Lee, Dae Young;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.61 no.4
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    • pp.371-377
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    • 2018
  • The fruits of Panax ginseng were extracted with 80% aqueous MeOH and the concentrates were partitioned into EtOAc, n-BuOH, and $H_2O$ fractions. The repeated $SiO_2$ and octadecyl $SiO_2$ column chromatographies for the EtOAc fraction led to isolation of five ginsenosides. The chemical structures of these compounds were determined as ginsenoside F1 (1), ginsenoside F2 (2), ginsenoside F3 (3), ginsenoside Ia (4), notoginsenoside Fe (5) based on spectroscopic analyses including nuclear magnetic resonance, MS, and infrared. Compounds 2-5 were isolated for the first time from the fruits of P. ginseng in this study. All isolated compounds were evaluated for cytotoxic activities against human cancer cell lines such as HCT-116, SK-OV-3, human cervix adenocarcinoma (HeLa), HepG2, and SK-MEL-5. Among them compounds 2, 4, and 5 showed significant cytotoxicity on cancer cells. Compound 2 exhibited cytotoxicity on SK-MEL-5, HepG2, and HeLa cells with $IC_{50}$ values of 82.8, 86.8, and $78.3{\mu}M$, respectively. Compound 4 showed cytotoxicity on HCT-116, SK-MEL-5, SK-OV-3, HepG2, and HeLa cells with $IC_{50}$ values of 24.5, 25.4, 26.3, 22.0, and $24.9{\mu}M$, respectively. Compound 5 did on SK-MEL-5 cell with $IC_{50}$ value of $81.7{\mu}M$. The cytotoxicity of ginsenoside 2, 4, and 5 isolated from the fruits of Panax ginseng showed strong inhibition effect against on cancer cells, all of which have a glucopyranosyl moiety on C-3.

Floristic Composition, Structure, and Dynamics of Koelreuteria paniculata Communities in Daegu City (대구시 자생 모감주나무군락의 종조성, 구조 및 동태)

  • Kim, Jun-Soo;Cho, Joon-Hee;Kim, Hak-Yun;Cho, Hyun-Je
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.168-176
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    • 2019
  • To understand the floristic composition, vegetation structure, and population dynamics of Koelreuteria paniculata communities, which are native to Daegu, South Korea, a field survey was conducted in July 2018 using phytosociological and complete enumeration methods. Based on information on vegetation and trees of >5 cm diameter at breast height collected during the field survey, we classified the community types by species composition and analyzed their vegetation strata, relative importance value (MIV), life forms, species diversity, and population structure. The community was divided into the following three types: Ulmus parvifolia-Vitex negundo var. incisa subcommunity, Securinega suffruticosa subcommunity, and Clematis apiifolia community. The vegetation unit system was organized into one community group, two communities, and two subcommunities. Vegetation coverage of the tree layer was >85%, while the herbaceous layer was <10%. MIV of K. paniculata appeared to be extremely high within all vegetation strata, with 94.3 of the tree layer, 81.6 of the subtree layer, 75.5 of the shrub layer, and 60.0 of the herbaceous layer. The species diversity (H') was significantly different among the community types, and the C. apiifolia community (2.062) was approximately four times higher than the S. suffruticosa subcommunity (0.547). The overall representative life form types were "$MM-R_5-D_4{\cdot}D_2-e$,", but there were some differences in the disseminule form among the community types. The population structure of K. paniculata showed the reverse J-shaped distribution with a high density of young individuals and low density of larger individuals among all three community types, and because no plant species within the lower vegetation could replace K. paniculata, it was considered to be a sustainable population.

This Study of the Arms Used in the Three Kingdoms (삼국시대(三國時代) 병기체제(兵器體制)의 연구(硏究))

  • Kim, sung-tae
    • Korean Journal of Heritage: History & Science
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    • v.34
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    • pp.20-58
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    • 2001
  • In order to unravel the characteristics of arms used in the 'Three Kingdoms,' Kokuryo, Silla and Paikje. the classification and the developing procedures of the arms should be first discussed. At first, the basic arms of the soldiers of Three Kingdoms were iron swords, iron spearheads, and bows. During that period, swords attached a ring pommel were commonly used. But after 5A. D. a sword with a decoration pommel appeared. Infantry generally used iron spearheads. From the late 4A. D. the long spearheads were broadly used in cavalry battles. In the late 6A. D. infantry mainly used long spearheads, and this resulted in the foundation of long spearheads units. There were two kinds of bows: Short Bow whose arch is small and Long Bow whose arch is long. It is known that the Short Bow was widely used in Kokuryo and Paikje up to 5A. D. In the early era, infantry used Long Bow, yet it was vastly used after 6A. D. when a castle's strategical value was great and defending a castle was. significant. Above mentioned, as basic combat weapons, iron spearhead and bow were fundamental. In particular, the spearhead was the essential weapon to a soldier. Yet, arrow gun and hook-shape cutters were important weapons. Especially, after 6A.D., when a castle became strategically pivotal in military, the arrow gun became the important weapon. This resulted in the foundation of arrow gun units. Hook-shape cutters were used to snatch horsemen or to climb up to fall the castle. Yet, the cutter was not the Three Kingodoms' basic weapon. In addition, the three stages of arms development in the Three Kingdoms are formation stage, development stage, and settlement stage. The formation stage was the period when premitive military unit appeared in the Three Kingdoms. It ranged from 1B. C. to the mid 3A. D. At that time according to regions. there were two weapon systems operating: North area including Kokuryo and the northern part of Paikje and South area including Silla, Kaya and the southern part of Paikje. ln North area a sword with a ring attached at the end of the holder, iron spear with neck and mid-size flat holder and iron arrowhead with an extension to fix, were used. In this period, during a war calvary units were mostly used and their weapon systems seemed possibly to succeed in that of Kochosun. In the development stage, when LoLang's influence on surroundings became weak, Koguryo, Paikjae and Silla had directly contacted each other. In the late 3A.D. to the early 6A.D., Silla achieved a drastic improvement in weapon system. This was the period when Kokuryo played a leading role in arms race. Kokuryo's arms manufacturing techniques passed onto Silla, Kaya and Paikje. In combat strategy a joint operation between infantry and calvary prevailed even if their military tactics were different. In a calvary battle heavily armed horsemen played import roles at this period. The horsemen and even horses were heavily guarded with iron armors. After all, the appearance of fully armed horsemen implies the very need of powerful destructive forces in weapon system. At that time, basic weapons were a big sword with a ring attached at the end of the holder, swallow's tail-shape spear with neck, and iron spearhead with neck and an extension. The settlement stage began at the mid 6A.D., when it was the revolutionary period in the arms development history. Of course, actual proofs and picture documents were not sufficient enough to penetrate full scale of the weapon system. But, according to historical circumstances and historic records, it is very certain that this period was the peak in arms development. In this period special military units, such as infantry-calvary companies, Archery units and Long spear units, that executed particular duties with special weapons, were founded. This became the characteristics of the settlement stage.

Emulsifying Properties of Gelatinized Octenyl Succinic Anhydride Modified starch from Barley (호화 옥테닐 호박산 전분의 유화 특성)

  • Kim, San-Seong;Kim, Sun-Hyung;Lee, Eui-Seok;Lee, Ki-Teak;Hong, Soon-Taek
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.1
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    • pp.174-188
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    • 2019
  • The present study was carried out to investigate the emulsifying properties of heat-treated octenyl succinic anhydride(OSA) starch and the interfacial structure at oil droplet surface in emulsions stabilized by heat-treated OSA starch. First, the aqueous suspensions of OSA starch were heated at $80^{\circ}C$ for 30 min. Oil-in-water emulsions were then prepared with the heat-treated OSA starch suspension as sole emulsifier and their physicochemical properties such as fat globule size, surface load, zeta-potential, dispersion stability, confocal laser scanning microscopic image(CLSM) were determined. It was found that fat globule size decreased as the concentration of OSA starch in emulsions increased, showing a lower limit value ($d_{32}:0.31{\mu}m$) at ${\geq}0.2wt%$. Surface load increased steadily with increasing OSA starch concentration in emulsions, possibly forming multiple layers. In addition, fat globule sizes were also influenced by pH: they were increased in acidic conditions and these results were interpreted in view of the change in zeta potentials. The dispersion stability by Turbiscan showed that it was more unstable in emulsions at acidic condition. Heat-treated OSA starch found to adsorb at the oil droplet surface as some forms of membrane (not starch granules), which might be indicative of stabilizing mechanism of OSA starch emulsions to be steric forces.

Study on the Application of Ultrasound Traits as Selection Trait in Hanwoo (한우 선발형질로써 초음파 형질의 활용방안 연구)

  • Choi, Tae Jeong;Choy, Yun Ho;Park, Byoungho;Cho, Kwang Hyun;Alam, M;Kang, Ha Yeon;Lee, Seung Soo;Lee, Jae Gu
    • Journal of agriculture & life science
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    • v.51 no.2
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    • pp.117-126
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    • 2017
  • Hanwoo young bulls are selected based on performance test using the weight at 12 months and pedigree index comprising marbling score. Pedigree index was not based on the progeny tested data but the breeding value of the proven bulls; resulting a lower accuracy. The progeny testing of the young bulls was categorized into testing at farm and at the test station. The farm tested data was difficult to compare with those from test station data. Farm tested bulls had different slaughter ages than those for test station bulls. Therefore, this study had considered a different age at slaughter for respective records on ultrasound traits. Records on body weight at 12 months, ultrasound measures at 12 and 24 months(uIMF, uEMA, uBFT, and uRFT), and carcass traits(CWT, EMA, BFT, and MS) were collected from steers and bulls of Hanwoo national improvement scheme between 2008 and 2013. Fixed effects of batch, test date, test station, personnel for measurement, personnel for judging, and a linear covariate of weight at measurement were fitted in the animal models for ultrasound traits. The ranges of heritability estimates of the ultrasound traits at 12 and 24 months were 0.21-0.43 and 0.32-0.47, respectively. Ultrasound traits at 12 and 24 months between similar carcass traits was genetically correlated at 0.52-0.75 and 0.86-0.89, respectively.

Analysis of Hydrodynamics in a Directly-Irradiated Fluidized Bed Solar Receiver Using CPFD Simulation (CPFD를 이용한 태양열 유동층 흡열기의 수력학적 특성 해석)

  • Kim, Suyoung;Won, Geunhye;Lee, Min Ji;Kim, Sung Won
    • Korean Chemical Engineering Research
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    • v.60 no.4
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    • pp.535-543
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    • 2022
  • A CPFD (Computational particle fluid dynamics) model of solar fluidized bed receiver of silicon carbide (SiC: average dp=123 ㎛) particles was established, and the model was verified by comparing the simulation and experimental results to analyze the effect of particle behavior on the performance of the receiver. The relationship between the heat-absorbing performance and the particles behavior in the receiver was analyzed by simulating their behavior near bed surface, which is difficult to access experimentally. The CPFD simulation results showed good agreement with the experimental values on the solids holdup and its standard deviation under experimental condition in bed and freeboard regions. The local solid holdups near the bed surface, where particles primarily absorb solar heat energy and transfer it to the inside of the bed, showed a non-uniform distribution with a relatively low value at the center related with the bubble behavior in the bed. The local solid holdup increased the axial and radial non-uniformity in the freeboard region with the gas velocity, which explains well that the increase in the RSD (Relative standard deviation) of pressure drop across the freeboard region is responsible for the loss of solar energy reflected by the entrained particles in the particle receiver. The simulation results of local gas and particle velocities with gas velocity confirmed that the local particle behavior in the fluidized bed are closely related to the bubble behavior characterized by the properties of the Geldart B particles. The temperature difference of the fluidizing gas passing through the receiver per irradiance (∆T/IDNI) was highly correlated with the RSD of the pressure drop across the bed surface and the freeboard regions. The CPFD simulation results can be used to improve the performance of the particle receiver through local particle behavior analysis.

Evaluation of waterlogging tolerance using chlorophyll fluorescence reaction in the seedlings of Korean ginseng (Panax ginseng C. A. Meyer) accessions (엽록소 형광반응을 이용한 인삼 유전자원의 습해 스트레스 평가)

  • Jee, Moo Geun;Hong, Young Ki;Kim, Sun Ick;Park, Yong Chan;Lee, Ka Soon;Jang, Won Suk;Kwon, A Reum;Seong, Bong Jae;Kim, Me-Sun;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.240-249
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    • 2022
  • Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

Effects of Urea and K2SO4 treatment on the mineral nutrient concentration and fruit skin color of 'Mibaekdo' peach fruits at harvest (Urea와 K2SO4 처리에 의한 복숭아 '미백도'에서 수확 시 과실의 무기성분 농도 및 과피색 변화)

  • Moon, B.W.;Yoon, I.K.;Moon, Y.J.;Nam, K.W.;Lee, Y.C.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.15 no.1
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    • pp.95-105
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    • 2013
  • This study has been conducted to investigate the effect of Urea and K2SO4 treatment at stone hardening stage and 20 days before harvest on soil chemical properties, mineral nutrient concentration and quality of 'Mibaekdo' fruit peach. K concentration after Urea and K2SO4 treatment in soil was increased significantly by Urea 162g+K2SO4 188g/tree(standard amount) treatment at stone hardening stage, K2SO4 1.0% tree-spray, Urea 81g+K2SO4 94g/tree(half amount), Urea 162g+K2SO4 188g/tree and Urea 324g+K2SO4 376g/tree(double amount) soil treatment before harvest 20 days compared to control. T-N, K and Ca concentration in leaf was increased significantly by all treatment. but Na concentration in leaf was increased by Urea 0.5% and K2SO4 1.0% tree-spray treatment before harvest 20 days. T-N concentration in fruit skin was increased significantly by standard amount soil treatment, which decreased by K2SO4 1.0% tree-spray and half amount soil treatment. T-N, K and Ca concentration in fruit flesh(1~10mm depth flesh from peel) were increased markedly by all treatment excepted Urea 0.5% tree-spray. The leaf weight at harvest was increased markedly by Urea 0.5% tree-spray, standard amount and double amount treatment before harvest 20 days. Fruit weight was increased significantly by standard amount compared to all treatment. Red fruit skin(Hunter a value) progress was effective by K2SO4 tree-spray, half amount and double amount treatment before harvest 20 days. Fruit SSC was increased significantly by Urea 0.5% and K2SO4 tree-spray before harvest 20 days, standard amount treatment at stone hardening stage compared to control.

Relative Toxicity of Abamectin to the redatoryMite Amblyseius womersleyi Schicha (Acari: Phytoseiidae) and Twospotted Spider MIte Tetranychus urticae Koch (Acari: Tetranychidae) (아바멕틴의 긴털이리응애(Amblyseius womersleyi Schicha)와 점박이응애(Tetranychus urticae Koch)에 대한 선택독성)

  • Park, C.G.;Lee, M.H.;Yoo, J.K.;Lee, J.O.;Choi, B.R.
    • Korean journal of applied entomology
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    • v.34 no.4
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    • pp.360-367
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    • 1995
  • The relative toxicity of abamectin was assessed to the predatory mite Amblyseius womersleyi Schicha and to dicofol-resistant and -susceptible twospotted spider mite (TSM) Tetranychus urticae Koch in the laboratory. Abamectin was much les toxic to the predator than to the spider mite. At 0.12 and 0.6 ppm, all TSM adult females of the tow strains were killed within 48 h after dipping n the solutions. The lower concentrations (0.06 and 0.012 ppm) killed more than 77% of TSM female adults of the two strains at 120 h after treatment. However, abmectin did not significantly affect the survival and mobility of A. womersleyi female adults at a concentration of 0.12 ppm but the mortality was slightly increased up to 20~23% at 0.6 and 6 ppm. Abamectin did not significantly affect hatchability of one-day old TSM eggs at 0.06~0.6 ppm. The Four-day old eggs were much more susceptible to abamectin than one-day old eggs were. Within 0.006-6 ppm, abamectin did not affect the hatchability of A. womersleyi eggs and the development of resulting immature predators. When the predator female adults were dipped in 0.6 and 0.12 ppm solution, their reproduction was not affected, but at 6 ppm it was decreased by 35%. However, the reproduction of TSM reduced significantly at concentrations between 0.006 and 0.6 ppm. The differential toxicity of abamectin between TSM and the predator could be of practical importance in managing spider mite populations in the field. Abamectin at selective sublethal concentrations (i.e., 0.012~0.06 ppm) could be of value in adjusting predator/prey ratios in integrated management of spider mites.

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Yield, Nitrogen Use Efficiency and N Uptake Response of Paddy Rice Under Elevated CO2 & Temperature (CO2 및 온도 상승 시 벼의 수량, 질소 이용 효율 및 질소 흡수 반응)

  • Hyeonsoo Jang;Wan-Gyu Sang;Youn-Ho Lee;Pyeong Shin;Jin-hee Ryu;Hee-woo Lee;Dae-wook Kim;Jong-tag Youn;Ji-Won Han
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.346-358
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    • 2023
  • Due to the acceleration of climate change or global warming, it is important to predict rice productivity in the future and investigate physiological changes in rice plants. The research aimed to explore how rice adapts to climate change by examining the response of nitrogen absorption and nitrogen use efficiency in rice under elevated levels of carbon dioxide and temperature, utilizing the SPAR system for analysis. The temperature increased by +4.7 ℃ in comparison to the period from 2001 to 2010, while the carbon dioxide concentration was held steady at 800 ppm, aligning with South Korea's late 21st-century RCP8.5 scenario. Nitrogen was applied as fertilizer at rates of 0, 9, and 18 kg 10a-1, respectively. Under conditions of climate change, there was an 81% increase in the number of panicles compared to the present situation. However, grain weight decreased by 38% as a result of reduction in the grain filling rate. BNUE, indicative of the nitrogen use efficiency in plant biomass, exhibited a high value under climate change conditions. However, both NUEg and ANUE, associated with grain production, experienced a notable and significant decrease. In comparison to the current conditions, nitrogen uptake in leaves and stems increased by 100% and 151%, respectively. However, there was a 25% decrease in nitrogen uptake in the panicle. Likewise, the nitrogen content and NDFF (Nitrogen Derived from Fertilizer) in the sink organs, namely leaves and roots, were elevated in comparison to current levels. Therefore, it is imperative to ensure resources by mitigating the decrease in ripening rates under climate change conditions. Moreover, there seems to be a requirement for follow-up research to enhance the flow of photosynthetic products under climate change conditions.