• The Journal of Korean Medicine
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• v.23 no.3
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• pp.145-163
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• 2002

Objectives: The purpose of this investigation was to evaluate the effects of Woohwangcheongsim-won and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). Methods: E20 cortical cells were dissociated in neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Woohwangcheongsim-won was added to the culture media for 24 hrs or 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Woohwangcheongsim-won's effects for neuronal death protection were evaluated by LDH assay, propidium iodide stain and phospho-H2AX immunostain and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family, PKCα, ca1pain I. Results & Conclusions : 1. This study indicated that Woohwangcheongsim-won's effects for neuronal death protection in hypoxia were confirmed by LDH assay, propidium iodide stain and phospho-H2AX immunostain in culture method of Embryonic day 20(E20) cortical neuroblasts. 2. Woohwangcheongsim-won's mechanisms for neuronal death protection in hypoxia are to reduce the membrane damage fraction, to restrain DNA truncate, to restrain inflow of cytochrome c into cellularity caused by Bak diminution, to reduce the caspase cascade intiator caspase-8 and the effector caspase-3, to reduce the calpain I activity and to increase PKCand its activity in the membrane fraction. (J Korean Oriental Moo 2002;23(3):145～163)

• BMB Reports
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• v.39 no.3
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• pp.247-252
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• 2006

A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.

• Journal of the Korean Geotechnical Society
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• v.16 no.3
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• pp.39-46
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• 2000

본 논문에서는 배수재가 설치된 구역과 설치되지 않은 구역으로 구성된 Olga-C 시험성토지반의 변형률속도 의존적인 압밀거동을 서술하였다. 배수재가 설치된 지반이 압밀거동에 대한 변형률속도의 영향을 해석하기 위하여 응력-변형률-변형률 속도의 관계식(v-$\varepsilon$v- v)을 이용한 축대칭 비선형 점소성 모델을 제안하였다. 제안된 모델은 실험실과 현장의 변형률속도 차이뿐만 아니라 간극수압의 소산과 생성의 복합적인 압밀과정을 고려할 수 있다. 연직 및 반경방향의 배수효과에 의해 배수재가 설치된 지반(Zone B)에서 유발되는 변형률 속도는 배수재가 설치되지 않은 연약지반 (Zone A)의 변형률 속도보다 크다. 유발된 변형률 속도의 영향으로 Zone B의 선행압밀하중도 Zone A에서 유발되는 선행압밀하중보다 크다. Olga-C 지역의 Zone A 에서는 응력완화효과가 유발되지만, Zone B에서는 응력완화효과가 유발 되지 않았다.

• The Journal of the Korean Society for Microbiology
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• v.22 no.3
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• pp.219-224
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• 1987

A strain of Leptospira interrogans(UM-19) was isolated in 1984 from a patient with epidemic pulmonary hemorrhagic fever. The bacteria has been characterized morphologically by a polymorphism showing spiral forms, short and long rods in culture. In order to study the biological relatedness among these forms of the bacteria, the short rods were separated from others by means of continuous sucrose density gradient centrifugation, and cultured at various temperatures such as $10^{\circ}C$, $15^{\circ}C$, $30^{\circ}C$ and $37^{\circ}C$. It was revealed that cultivation or subcultivation of the short rods has resulted in morphological changes observed by darkfield examination, silver stain, and scanning electron microscope showing spiral forms as well as short and long rods.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1490-1491
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• 2007

The salt contamination on the distribution facilities, such as suspension insulator, line post insulator, lightning arrester, COS, is one of the major coastal areas because more than 90% of salt contaminated failure occurs in distribution facilities. Did to stain distribution facilities artificially according to Equivalent Salt Deposit Density(ESDD) grade. The electrical test were performed on the non-polluted and polluted facilities(ESDD B, C, D grade).

• Microbiology and Biotechnology Letters
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• v.25 no.5
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• pp.468-476
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• 1997

Soft-rot bacterial pathogen, Erwinia sp., was isolated from chinese cabbage tissue showing soft-rot symptom. This bacterial strain caused soft-rot to chinese cabbage and potato, and it was identified as Erwinia carotovora subsp. carotovora LY34 (E. c. subsp. carotovora LY34). Erwinia carotovora subsp. carotovora LY34 did not have hemicellulase but extracellular cellulase, pectinase, polygalacturonase, protease activity. The results of the microscopy showed that chinese cabbage tissue and potato tissue were macerated by infection of E. c. subsp. carotovora LY34. In analysis of the cellulases activity of the isolated cellulose-degradation enzymes from E. c. subsp. carotovora LY34 total protein, three cellulase activity bands were detected by non-denaturation gel electrophoresis method and five cellulase activity bands were detected by CMC-SDS-PAGE direct stain method.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.07b
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• pp.582-585
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• 2004

Pb0.5Sr0.5TiO3 (PST) thin films were fabricated by the alkoxide-based sol-gel process using spin-coating method on Pt/Ti/SiO2/Si substrate. The PST films annealed from 500C to 650C for 1h show a perovskite phase and dense microstructure with a smooth surface. The grain size and dielectric constant of PST films increases with the increase in annealing temperature, which reduces the SiO2 equivalent thickness of the PST film. The crystallinity or internal strain in the PST thin films analyzed from the diffraction-peak widths correlate well with the decrease in the dielectric losses. The dielectric constants and dielectric loss (%) of the PST films annealed at 650c (teq : 0.89 nm) were 549 and 0.21%, respectively.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1996.10a
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• pp.139-146
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• 1996

The torsion tests in the range of 900~1100$^{\circ}C$ and 5.0X10-2~5.0X100/sec were performed to study the high temperasture deformation behavior kinetics of 304 stainless steels. The flow curves and microstructures exhibited the characteristic of dynamic recrystallization(DRX). The relationship between the critical strain($\varepsilon$c) for the initiation of dynamic recrystallization and the peak strain($\varepsilon$p) could be expressed as $\varepsilon$c=0.73$\varepsilon$p. The dependence of the flow stress on temperature(T) and stain rate($\varepsilon$) was expressed by hyperbolic sine law, $\varepsilon$=2.75X1014 (sinh 0.076$\sigma$)5.26 exp(-379.55kJ/mol). Under the Zener-Hollomon parameter, Z value of 1013 order, it was found that the grain size was 20${\mu}$m. The relationship between the grain size, dDRX and Z parameter was expressed as dDRX =139.48-7.33 log Z.

• KSBB Journal
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• v.30 no.2
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• pp.58-62
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• 2015

A 3-weeks feeding trial with 288 laying hens were conducted to determine the efficacy of lecithin-free egg yolk at different levels of dietary Ca on performance and Ca absorption. Laying hens were divided into 6 groups according to calcium level and testing agent; 0% calcium feed (A), 0.2% calcium feed (B), 0.4% calcium feed (C, normal feed), 0.6% calcium feed (D), 0.4% calcium feed + 0.2% egg byproduct (C+0.2), 0.4% calcium feed + 0.4% egg byproduct (C+0.4). The final body weight gain of C+0.2 and C+0.4 groups were higher by 1.5% and 7.4% respectively than group C. Tibia ash contents did not show significantly difference, but calcium contents increase (p<0.05) in C+0.2 and C+0.4 groups. Parallel undecalcified tibia joint sections were stained for calcium absorption by the von Kossa's stain. This result show that lecithin free egg byproduct supplementation to normal calcium feed improved growth performance and calcium utilization in laying hens.

• Applied Chemistry for Engineering
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• v.8 no.6
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• pp.1014-1021
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• 1997

Studies were carried out on the preparation of several kinds of pulps from Sponge gourd fiber by KP, ASP, SP PAP, AP and addition of AQ pulping process. These unbeaten and beaten pulping fibers were observed their characteristics and fiber structure by SEM, FQA, Image analyzer and Micro projector. The results were summarized as follows; 1) The cooking condition which is the possible defibrilation of Shives are KP base($160^{\circ}C$, 2hr.), ASP base($155^{\circ}C$, 4hr.), PAP base($160^{\circ}C$, 1hr.). From the results, the kappa no. had the range of 12, 25, 10 each other. 2) The pulp yields of sponge gourd fiber obtained the range of KP 50~55%, ASP&60~70% and PAP 45~50%. SP base have the highest and contnets of KP&PAP base are much the same as woods. 3) Increasing amount of NaOH on Pulping was accelerated the defibrilation of Shives and was changed a morphology of pulping fiber quality such as fiber length, curl and kink index. 4) Addition of AQ on pulping process of sponge gourd fiber had a affect to raise the rate of delignification while protecting cellullosic components against degradation, especially defibrilation was very excellent, beated pulp much more easily and increased the fibrilation. 5) ASP system have higher bulk density, fiber bonding and protecting cellullosic components against degradation than KP or PAP. 6) The color reactions of the "C" stain solution showed blue or blue-gray with clean and transparency thin cell wall.