The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Relatedness Between Different Morphologies of L. interrogans Isolated from a Patient with Epidemic Pulmonary Hemorrhagic Fever

유행성 폐출혈열 환자에서 분리된 병원체에서 관찰된 다양한 형태들간의 연관성

  • Lee, Tae-Yoon (Department of Microbiology, College of Medicine, Yonsei University) ;
  • Kim, Kyung-Won (Department of Microbiology, College of Medicine, Yonsei University) ;
  • Kim, Gye-Sung (Department of Microbiology, College of Medicine, Yonsei University)
  • 이태윤 (연세대학교 의과대학 미생물학교실) ;
  • 김경원 (연세대학교 의과대학 미생물학교실) ;
  • 김계성 (연세대학교 의과대학 미생물학교실)
  • Published : 1987.09.30
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Abstract

A strain of Leptospira interrogans(UM-19) was isolated in 1984 from a patient with epidemic pulmonary hemorrhagic fever. The bacteria has been characterized morphologically by a polymorphism showing spiral forms, short and long rods in culture. In order to study the biological relatedness among these forms of the bacteria, the short rods were separated from others by means of continuous sucrose density gradient centrifugation, and cultured at various temperatures such as $10^{\circ}C$, $15^{\circ}C$, $30^{\circ}C$ and $37^{\circ}C$. It was revealed that cultivation or subcultivation of the short rods has resulted in morphological changes observed by darkfield examination, silver stain, and scanning electron microscope showing spiral forms as well as short and long rods.

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