Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

분리균 Erwinia carotovora subsp. carotovora LY34의 병원성 및 CMCase Isozymes 생성

  • Published : 1997.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Soft-rot bacterial pathogen, Erwinia sp., was isolated from chinese cabbage tissue showing soft-rot symptom. This bacterial strain caused soft-rot to chinese cabbage and potato, and it was identified as Erwinia carotovora subsp. carotovora LY34 (E. c. subsp. carotovora LY34). Erwinia carotovora subsp. carotovora LY34 did not have hemicellulase but extracellular cellulase, pectinase, polygalacturonase, protease activity. The results of the microscopy showed that chinese cabbage tissue and potato tissue were macerated by infection of E. c. subsp. carotovora LY34. In analysis of the cellulases activity of the isolated cellulose-degradation enzymes from E. c. subsp. carotovora LY34 total protein, three cellulase activity bands were detected by non-denaturation gel electrophoresis method and five cellulase activity bands were detected by CMC-SDS-PAGE direct stain method.

Keywords

References

  1. J. Bacteriol. v.168 no.2 Nucleotides sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology Fukumori, F.;N. Sashihara;T. Kudo;K. Horikoshi
  2. J. Bacteriol. v.172 no.3 Molecular cloning, expression, and characterization of e-[p ndo-β-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans Baird, S. D.;D. A. Johnson;V. L. Seligy
  3. J. Bacteriol. v.162 no.1 Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum Beguin, P.;P. Cornet;J. -P. Aubert
  4. FEMS Microbiol. Lett. v.16 Cloning and expression in E.coli of Clostridium thermocellum genes coding for amino acid synthesis and cellulose hydrolysis Cornet, P.;D. Tronik;J. Millet;J. -P. Aubert
  5. Mol. Gen. Genet. v.213 The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa Hall, J.;H. J. Gilbert
  6. J. Bacteriol. v.174 Role of the two component leader sequence and mature amino acid sequence in extracellular export of endoglucanase EGL from Pseudomonas solanacearum Huang, J.;M. A. Schell
  7. Gene v.44 Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi Wong, W. K. R.;B. Gerhard;Z. M. Guo;D. G. Kilburn;R. Anthony;J. Warren;Jr. R. C. Miller
  8. Biochemistry v.33 The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: Evidence from detailed kinetic studies of mutants MacLeod, A.;T. Lindhorst;S. G. Withers;R. A. J. Warren
  9. Gene v.89 Nucleotide sequence of the engXCA gene encoding the major endoglucanase of Xanthomonas campestris pv. campestris Gough, C. L.;J. M. Dow;J. Keen;B. Henrissat;M. J. Daniels
  10. Mol. Gen. Genet. v.198 Evidence for clustered pathogenicity genes in Xanthomonas campestris pv. campestris Turner, P.;C. Batber;M. Daniels
  11. Ann. Rev. Phytopathol. v.25 Molecular genetics of pathogenesis by soft-rot Erwinia Kotoujansky, A.
  12. Mol. Plant. Pathology v.77 Cloning of genes for Erwinia carotovora subsp. carotovora pectolytic enzymes and further characterization of the polygalacturonases Willis, J.;J. K. Engwall;A. K. Chatterjee
  13. Ann. Rev. Phytopathol. v.26 Evolution of concepts for chemical control of plant disease Baldwin, B. C.;W. G. Rathmell
  14. Ann. Rev. Phytopathol. v.24 The role of pectic enzymes in plant pathogenesis Collmer, A.;N. T. Keen
  15. Ann. Rev. Phytopathol. v.26 Molecular genetics of phytopathogenicity in phytopathogenic bacteria Daniels, M. J.;J. M. Dow;A. E. Osbourn
  16. Mol. Plant-Microbe Inter. v.4 no.3 Molecular cloning of an aepA gene that activates production of extracellular pectolytic, cellulolytic, and proteolytic enzymes in Erwinia carotovora subsp. carotovora Murata, H.;L. McEvoy;A. Chatterjee;A. Collmer;A. K. Chatterjee
  17. J. Bacteriol. v.164 no.1 recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp-carotovora Zink, R. T.;J. K. Engwall;J. L. McEvoy;A. K. Chatterjee
  18. Mol. Plant-Microbe Inter. v.6 no.3 Characterization of a novel regulatory gene aepA that controls extracellular enzyme production in the phytopathogenic bacterium Erwinia carotovora subsp. carotovora. Liu, Y.;H. Murata;A. Chatterjee;A. K. Chatterjee
  19. Gene v.90 CelS: a novel endoglucanase identified from Erwinia carotovora subsp Saarilahti, H. T.;B. Henrissat;E. T. Palva
  20. Mol. Gen. Genet. v.247 Structure and regulation of the Erwinia carotovora subsp. carotovora SCC3193 cellulase gene celV1 and the role of cellulase in phytopathogenicity Mae, A.;R. Heikinheimo;E. T. Palva
  21. Mol. Plant-Microbe Inter v.6 no.2 Induction of plant defence response by exoenzymes of Erwinia carotovora subsp. carotovora Palva, T. K.;K. O. Hoimstrom;P. Heino;E. T. Palva
  22. Appl. Environ. Microbiol. v.43 no.4 Use of Congo red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen Teather, M. R.;P. J. Wood
  23. Phytopathol v.69 Erwinia chrysanthemi: A comparative study phenotypic properties of strains from several hosts and other Erwinia species Dickey, R. S.
  24. Phytopathol v.71 Identification and nutritional differentiation of the Erwinia sugar beet pathogen from members of Erwinia carotovora and Erwinia chrysanthemi Thomson, S. V.;D. C. Hildebrand;M. N. Schroth
  25. Laboratory guide for identification of plant pathogenic bacteriology Schaad, N. W.
  26. N. J. J. Sci. v.12 A taxonomic study of the genus Erwinia Ⅱ. the carotovora group Dye, D. W.
  27. Bergey's manual of systematic bacteriology v.3 Holt, J. G.
  28. Phytopathol v.74 Scaning E. M of Virulent and avirulent strains of X. c. pv. oryzae Mew, T. W.;Mew, I.;J. S. Huang
  29. Electron microscopy Jones, B. R.
  30. Ph D. Dissertation. The Graduate School of Geongsang National Univ. Molecular cloning and characterization of cellulose-degradation-enzyme in Erwinia carotovora subsp. carotovora LY34 Lim S. T.
  31. Mol. Gen. Genet. v.214 Cloning, sequencing and expression of a Bacillus bacteriolytic enzyme in Escherichia coli Povin, C.;Leclere, D.;Tremblay, G.;Asselin, A.;Bellemare, G.
  32. EMBO J v.4 Molecular cloning of Erwinia chrysanthemi pectinase structural genes Kotoujansky, A.;A. Diolez;M. Boccara;Y. Bertheau;T. Ando;A. Coleno
  33. J. Bacteriol v.179 Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: Enzyme characteristic and potential inhibitors Tardy, F.;Nasser, W.;Baudouy, R. J.;Pattat N. H. C.
  34. Mol. Gen. Genet. v.209 Resolution of four pectate lyase structural genes of Erwinia chrysanthemi(EC16) and characterization of the enzymes produced in Escherichia coli Barras, F.;K. K. Thurn;A. K. Chatterjee