• Title/Summary/Keyword: C-flux

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Central energy metabolism remains robust in acute steatotic hepatocytes challenged by a high free fatty acid load

  • Niklas, Jens;Bonin, Anne;Mangin, Stefanie;Bucher, Joachim;Kopacz, Stephanie;Matz-Soja, Madlen;Thiel, Carlo;Gebhardt, Rolf;Hofmann, Ute;Mauch, Klaus
    • BMB Reports
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    • v.45 no.7
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    • pp.396-401
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    • 2012
  • Overnutrition is one of the major causes of non-alcoholic fatty liver disease (NAFLD). NAFLD is characterized by an accumulation of lipids (triglycerides) in hepatocytes and is often accompanied by high plasma levels of free fatty acids (FFA). In this study, we compared the energy metabolism in acute steatotic and non-steatotic primary mouse hepatocytes. Acute steatosis was induced by pre-incubation with high concentrations of oleate and palmitate. Labeling experiments were conducted using [$U-^{13}C_5$,$U-^{15}N_2$] glutamine. Metabolite concentrations and mass isotopomer distributions of intracellular metabolites were measured and applied for metabolic flux estimation using transient $^{13}C$ metabolic flux analysis. FFAs were efficiently taken up and almost completely incorporated into triglycerides (TAGs). In spite of high FFA uptake rates and the high synthesis rate of TAGs, central energy metabolism was not significantly changed in acute steatotic cells. Fatty acid ${\beta}$-oxidation does not significantly contribute to the detoxification of FFAs under the applied conditions.

Pervaporative Recovery of Bio-butanol through Dense- and Composite-type PDMS Membranes (PDMS막을 이용한 바이오 부탄올 분리정제와 투과증발 특성에 관한 연구)

  • Kim, Jeong-Hoon;Ryu, Bi-Ho;Chang, Bong-Jun;Kim, Woo-Nyon
    • Membrane Journal
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    • v.19 no.2
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    • pp.157-164
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    • 2009
  • Bio-butanol recovery by pervaporation was performed with dense and composite polydimethylsiloxane (PDMS) membranes. The pervaporative behavior of the membranes was investigated as a function of operation temperature $(20{\sim}40^{\circ}C)$ and membrane thickness $(100{\sim}1{\mu}m)$ using a series of aqueous BtOH model solutions $(1{\sim}5wt%)$. With the increment of the BtOH concentration in feed, the Butanol concentration in permeate, pervaporation selectivity of Butanol over water and Butanol permeation flux increased. As the operating temperature of feed solutions increased, the BtOH concentration in permeate, pervaporation selectivity and permeation flux increased markedly. As the thickness of the PDMS membrane decreased, permeation flux increased but pervaporation selectivity decreased. These results were explained in terms of high solubility and low diffusion resistance of BtOH over water toward hydrophobic and rubbery PDMS membranes.

CRITICAL HEAT FLUX ENHANCEMENT IN FLOW BOILING OF Al2O3 AND SiC NANOFLUIDS UNDER LOW PRESSURE AND LOW FLOW CONDITIONS

  • Lee, Seung-Won;Park, Seong-Dae;Kang, Sa-Rah;Kim, Seong-Man;Seo, Han;Lee, Dong-Won;Bang, In-Cheol
    • Nuclear Engineering and Technology
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    • v.44 no.4
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    • pp.429-436
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    • 2012
  • Critical heat flux (CHF) is the thermal limit of a phenomenon in which a phase change occurs during heating (such as bubbles forming on a metal surface used to heat water), which suddenly decreases the heat transfer efficiency, thus causing localized overheating of the heating surface. The enhancement of CHF can increase the safety margins and allow operation at higher heat fluxes; thus, it can increase the economy. A very interesting characteristic of nanofluids is their ability to significantly enhance the CHF. Nanofluids are nanotechnology-based colloidal dispersions engineered through the stable suspension of nanoparticles. All experiments were performed in round tubes with an inner diameter of 0.01041 m and a length of 0.5 m under low pressure and low flow (LPLF) conditions at a fixed inlet temperature using water, 0.01 vol.% $Al_2O_3$/water nanofluid, and SiC/water nanofluid. It was found that the CHF of the nanofluids was enhanced and the CHF of the SiC/water nanofluid was more enhanced than that of the $Al_2O_3$/water nanofluid.

Arsenic removal from drinking water by direct contact membrane distillation

  • Zoungrana, Ali;Zengin, Ismail H.;Elcik, Harun;Yesilirmak, Dilara;Karadag, Dogan;Cakmakci, Mehmet
    • Membrane and Water Treatment
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    • v.7 no.3
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    • pp.241-255
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    • 2016
  • In this study, the treatability of arsenic (As) contaminated water by direct contact membrane distillation (DCMD) at different delta temperatures (${\Delta}T$) 30, 40 and $50^{\circ}C$ has been investigated. Two different pore sizes ($0.22{\mu}m$ and $0.45{\mu}m$) of hydrophobic membranes made of polyvinylidene fluoride (PVDF) were used. The membrane pore sizes, the operating temperatures, the feed solution As concentration and conductivity have been varied during the experimental tests to follow the removal efficiency and flux behavior. Both membranes tested had high removal efficiency of arsenite (As (III)) and arsenate (As (V)) and all permeates presented As concentration far lower than recommended $10{\mu}g/L$ of world health organization (WHO). As concentration was below detection limit in many permeates. Conductivity reduction efficiency was over 99% and the transmembrane flux (TMF) reached $19L/m^2.h$ at delta temperature (${\Delta}T$) of $50^{\circ}C$ with PVDF $0.45{\mu}m$ membrane. The experimental results also indicated that $0.45{\mu}m$ pore sizes PVDF membranes presented higher flux than $0.22{\mu}m$ pore sizes membranes. Regardless of all operating conditions, highest fluxes were observed at highest ${\Delta}T$ ($50^{\circ}C$).

Surface morphology and deuterium retention in W and W-HfC alloy exposed to high flux D plasma irradiation

  • Yongkui Wang;Xiaochen Huang;Jiafeng Zhou;Jun Fang;Yan Gao;Jinlong Ge;Shu Miao;Zhuoming Xie
    • Nuclear Engineering and Technology
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    • v.55 no.2
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    • pp.575-579
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    • 2023
  • In this work, pure W and W-0.5wt%HfC alloy (WHC05) were fabricated by sintering and hot-rolling following the same processing route. After exposing to a high flux deuterium plasma irradiation with the D+ flux to three fluences of 6.00 × 1024, 2.70 × 1025 and 7.02 × 1025 D/m2, the evolution of surface morphology, deuterium retention and hardening behaviors in pure W and WHC05 has been studied. The SEM results show the formation of D blisters on the irradiated area, and with the increase of D implantation, the size of these blisters increases from 200 ~ 500 nm (2.70 × 1025 D/m2) to 1 ~ 2 ㎛ (7.02 × 1025 D/m2) in WHC05 and from 1 ~ 2 ㎛ (2.70 × 1025 D/m2) to > 3 ㎛ (7.02 × 1025 D/m2) in pure W, respectively. A higher D retention and obvious hardening are observed in pure W than that of the WHC05 alloy, indicating an improve radiation resistance in WHC05 compared to pure W.

Use of Response Surface Methodology for Optimization of Clarified Mixed Apple and Carrot Juice Production (반응표면 분석을 이용한 사과.당근 혼합주스의 청징공정 최적화)

  • Seog, Eun-Ju;Lee, Jun-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.8
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    • pp.1051-1056
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    • 2006
  • Response surface methodology was used to investigate the quality of clarified mixed apple and carrot juices using ultrafitration. Apple and carrot juices were blended at the ratio of 1:3, 1:1, and 3:1. A three-variable, three-level central composite design was employed where the independent variables were the blend ratio, temperature and average transmembrane pressure (ATP). With increasing temperature and pressure, flux linearly increased regardless of blending ratio. Blend juice with 75% apple showed the highest soluble sugar and total sugar content in apple and carrot blend juices. Soluble solid contents were more affected by blending ratio than temperature and ATP. Total sugar contents were greatly affected by temperature; increasing temperature led to higher total sugar content up to $25^{\circ}C$. Higher carrot ratio led to higher vitamin C content. In general, higher acidity was achieved by higher apple content and acidity was increased with increasing temperature. Turbidity increased for all samples as APT increased, with the blending ratio of 1:1 (apple:carrot) showing the highest turbidity. Viscosity was greatly changed in the blending ratio of 3:1 (apple:carrot) juice. The polynomial models developed by RSM were satisfactory to describe the relationships between the studied factors and the responses. Analytical optimization gave $flux=0.216\;L/m^2.h$, soluble $solids=10.39^{\circ}Brix$, total sugar=71.32 mg/mL, vitamin C=315.18 mg%, acidity=7.78 mL, turbidity=0.017, and viscosity=1.44 cp, when using a $temperature=44.97^{\circ}C$, ATP=113.57 kPa, and blend ratio=28.50%.

Magnetic Properties of the $Y_{1}Ba_{2}Cu_{3-x}Sn_{x}O_{7-y}$ $High-T_{C}$ Superconductor (고온 초전도체 $Y_{1}Ba_{2}Cu_{3-x}Sn_{x}O_{7-y}$의 자기적 특성 연구)

  • 이성규;이주일;김문석;유성초;임우영;백종성
    • Journal of the Korean Magnetics Society
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    • v.1 no.2
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    • pp.15-21
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    • 1991
  • The magnetic properties of the $Y_{1}Ba_{2}Cu_{3-x}Sn_{x}O_{7-y}$ superconductor were studied as a function of Sn concentration by utilizing both the vibrating sample magnetometer (VSM) and torque magnetometer. Unlike the cases where Fe and Co were substituted for Cu, the superconducting transition temperature was maintained above 90 K until x reached the value of 0.36. The lower critical field $H_{c1}(T)$ and upper critical field $H_{c2}(T)$ are measured as a function of temperature and external magnetic field, respectively. By aid of these results, $H_{c1}(0)$.($H_{c2}(0)$), the coherence length ${\varepsilon}_{0}$, the penetration depth ${\lambda}_{0}$, and the Ginzburg-Landau parameter k were oqtained. Flux pinning was also observed in the sample.

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Dry Magnetic Particle Inspection of Ingot Cast Billets (강편 빌레트의 건식 자분 탐상)

  • Kim, Goo-Hwa;Lim, Zhong-Soo;Lee, Eui-Wan
    • Journal of the Korean Society for Nondestructive Testing
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    • v.16 no.3
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    • pp.162-173
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    • 1996
  • Dry magnetic particle inspection(MPI) was performed to detect the surface defects of steel ingot cast billets. Magnetic properties of several materials were characterized by the measurement of the B-H hysteresis curve. The inspection results were evaluated in terms of the magnetizing current, temperature, and the amount of magnetic particles applied to billets. Magnetic flux leakage near the defect site of interest was measured and compared with the results of calculation by the finite element method in the case of direct magnetizing current. Direct and alternating magnetizing currents for materials were deduced by the comparison of the inspections. Results of the magnetic particle inspection by direct magnetizing current were compared with those of finite element method calculations, which were verified by measuring magnetic leakage flux above the surface and the surface defects of the material. For square rods, due to the geometrical effect, the magnetic flux density at the edges along the length of the rods was about 30% of that at the center of rod face for a sufficiently large direct magnetizing current, while it was about 70% for an alternating magnetizing current. Thus, an alternating magnetizing current generates rather uniform magnetic flux density over the rods, except for the region on the face across about 10 mm from the edge. The attraction of the magnetic particle by the magnetic leakage field was nearly independent of the surface temperature of the billets up to $150^{\circ}C$. However, the temperature should have been limited below $60^{\circ}C$ for an effective fixing of gathered magnetic particles to the billet surface using methylene chloride. We also found that the amount of applied magnetic particles tremendously affected the detection capability.

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Characteristics on the Permeation of Protein Through Membrane of Ultrafiltration Reactor (한외여과막 반응기에서 단백질의 막 투과특성)

  • 변희국;전유진;김세권
    • Membrane Journal
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    • v.8 no.1
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    • pp.42-49
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    • 1998
  • Effects of operating time, pH, temperature, concentration and addition of proteolytic enzyme on permeate flux for the ultrafiltration of gelatin and bovine serum albumin(BSA) solutions were studied. The results showed that permeate flux of gelatin solution was maintained almost constant during the operating time, and that of BSA solution was decreased to 66% of the initial value after 40 min operation. The permeate flux of gelatin solution was increased by increasing temperature. The permeate flux of BSA solution was constant in the temperature range of 30~$50{\circ}C$, but increased at $60{\circ}C.$. The permeate fluxes of gelatin and BSA solution showed minimum values near the isoelectric point of pH 5.0. The permeate fluxes of 1%(w/v) and 6% gelatin solution were $43.0l/m^2\cdot hr$ and $13.5l/m^2\cdot hr$, respectively. Those of 1% and 4% BSA solution were $33.0l/m^2\cdot hr$ and $14.0l/m^2\cdot hr$, respectively. The permeate fluxes of gelatin and BSA solutions were decreased to 68.6% and 57.6% of their initial values by increasing their concentration, respectively. The permeate fluxes of gelatin and BSA solutions were enhanced by 30% with the addition of proteolytic enzyme.

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Seasonal Variability of Thermal Structure and Heat Flux in the Juam Reservoir (주암호의 계절별 수온 구조와 열수지 변화)

  • Sun, Youn-Jong;Cho, Cheol;Kim, Byong-Chun;Huh, In-Aa;Yoon, Jun-Heon;Chang, Nam-Ik;Cha, Sung-Sik;Cho, Yang-Ki
    • Korean Journal of Ecology and Environment
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    • v.36 no.3 s.104
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    • pp.277-285
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    • 2003
  • Temperature profiles were observed to understand seasonal variation of thermal structures in the Juam reservoir from March 2000 to May 2001. Heat flux which affects thermal structures was calculated by observed water temperature and meteorological data. Temperature became homogeneous vertically by convection due to the surface cooling in winter. Maximum heat loss through the surface (109.45W/$m^2$) occurred in December. There was a horizontal gradient of water temperature in winter. The temperature was $3^{\circ}C$ at upstream and $5^{\circ}C$ near the dam. The surface temperature increased by the increase of solar radiation in spring and summer. Maximum heat gained through the surface was 101.95 W/$m^2$ in July. Maximum surface temperature was $29^{\circ}C$ in August, whereas the bottom water was $7^{\circ}C.$ Surface mixed layer became thicker and its temperature decreased by surface heat loss in fall and winter.