• Animal cells and systems
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• v.2 no.1
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• pp.117-122
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• 1998

D-raf, a Drosophila homolog of the human c-raf-1, is known as a signal transducer in cell proliferation and differentiation. A previous study found that the D-raf gene expression is regulated by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system. In this study, we found the sequences homologous to transcription factor C/EBP, MyoD, STAT and Myc recognition sites in the D-raf promoter. We have generated various base substitutional mutations in these recognition sites and subsequently examined their effects on D-raf promoter activity through transient CAT assays in Kc cells with reporter plasmids p5'-878DrafCAT carrying the mutations in these binding sites. Through gel mobility shift assay using nuclear extracts of Kc cells, we detected factors binding to these recognition sites. Our results show that transcription factor C/EBP, STAT and Myc binding sites in D-raf promoter region play a positive role in transcriptional regulation of the D-raf gene and the Myo D binding site plays a negative role.

• Journal of the Korean Ceramic Society
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• v.23 no.6
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• pp.66-70
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• 1986

The calcining temperature ranging from 900$^{\circ}C$ to 1300$^{\circ}C$ affected on the planar coupling factor and resonance characteristics of BaTiO3 ceramics doped with 0.2 wt% MnO2 have been investigated. Dielectric constant planar coupling factor and anti-resonance frequency of the sample increased with the calcining temperature up to 1,200$^{\circ}C$ and decreased above that temperature but the resonance frequency decreased slightly with the increasing calcining temperature. The planar coupling factor and anti-resonance frequency increased with the sintered density and dielectric constant while the resonance frequency was almost constant. The resonance and anti-resonance frequency increased with the sample temperature.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.2 s.95
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• pp.213-218
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• 2005

It is well known that the loss factor and Young's modulus are fundamental mechanical properties of materials. Recently, the use of complex plastics is increasing for vibration proof. In this study, we evaluated two mechanical values of polycarbonate and acrylonitrile butadiene styrene by using two different standard test methods of ASTM E 756 and ISO 6721. Because damping properties of material generally depend on temperature, test specimen‘s temperature were controlled in the temperature range between - $10^{\circ}C\;and\;60^{\circ}C$. The results shown that the loss factor of polycarbonate gradually increased as increasing temperature, while the Young's modulus decreased. However, the loss factor and the Young's modulus of acrylonitrile butadiene styrene are varied somewhat at $60^{\circ}C$.

• Membrane Journal
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• v.14 no.4
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• pp.312-319
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• 2004

The porous SiO$_2$-B$_2$O$_3$ membrane was prepared from Si(OC$_2$$H_5$)$_4$-($CH_3$O)$_3$B-C$_2$$H_5$OH-$H_2O$ system by sol-gel method. In order to investigate the characteristics of this membrane, we examined that using BET, IR spectrophotometer, X-ray diffractometer, SEM and TEM. At $700^{\circ}C$, the surface area of SiO$_2$-B$_2$O$_3$ membrane was 354.398 $m^2$/, the median pore diameter was 0.0048 ${\mu}{\textrm}{m}$, and the particle size of SiO$_2$-B$_2$O$_3$ membrane was 7 nm. The separation properties of the gas mixture ($H_2$/$N_2$) through the SiO$_2$-B$_2$O$_3$ membrane was studied as a function of pressure. The real separation factor($\alpha$) of SiO$_2$-B$_2$O$_3$ membrane for $H_2$/$N_2$ gas mixture was 4.68 at 155.15 cmHg and $25^{\circ}C$. The real separation factor($\alpha$), head separation factor($\beta$) and tail separation factor((equation omitted)) were increased as the pressure of permeation cell increased.

• Journal of Fashion Business
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• v.10 no.5
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• pp.58-73
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• 2006

The purpose of this study was to improve of fittness and coverage rate of exporting domestic clothing to China for Chinese Adult Female. For study, It was measured 1381 female women aged between 19 and 50 who resident in Beijing and Shanghai in China. as a sample, 1360 female women was seleted for development of apparel sizing system. As for the method of this study was made of 111 items by indirect measures done during Jun. 23 $\sim$ Aug. 7, 2004. Data analysis were processed by SPSS WIN 10.0 Program was used to for technical statistical analysis, correlation analysis, factor analysis, ANOVA(t-test and F-test), duncan's multiple test. The result was as follows: Chinese Adult women's constitutional components determined by factor analysis, six components could be identified: factor 1:constitutional obesty and width size, factor 2: longistudinal body size, factor 3: shoulder form and size, factor 4: longistudinal upper body size, factor 5: under body size, factor 6: shoulder dropping. According to the Women's Wear Specifications(GB/T 1335.2-1997) by drop, body types of Chinese Adult female was classified into six types, it was Y, A, B, C and Z, D. In order, A type as standard somatotype(49.8%), B type(26.9%), Y type (18.8%), C type(2.9%). Y type had the average height and shoulder, bust girth. They were slimmer in overall terms. A type had a normal obesity and body size in height, shoulder, bust girth. They were average Chinese Women. B type had a smaller then A type. They were more obes and thick waist girth. C type had the highest obesity, the widest shoulder and bust girth, and thick waist girth. The characteristics of each body type following the body type structure factor are Y-type for slender type, A-type for standard type, B-type for slightly large type, and C-type for obese type. For each region, the Beijing area had in the order of A-type, Y-type, B-type and C-type, and the Shanghai area had in the order of A-type, B-type, Y-type and C-type.

• Journal of Life Science
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• v.27 no.8
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• pp.879-887
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• 2017

It is well established that brain-derived neurotrophic factor (BDNF) is expressed not only in the brain but also in skeletal muscle, and is required for normal neuromuscular system function. Histone deacetylases (HDACs) and insulin-like growth factor-I (IGF-I) are potent regulators of skeletal muscle myogenesis and muscle gene expression, but the mechanisms of HDAC and IGF-I in skeletal muscle-derived BDNF expression have not been examined. In this study, we examined the effect of IGF-I and suberoylanilide hydroxamic acid (SAHA), a pan-HDAC inhibitor, on BDNF induction. Proliferating or differentiating C2C12 skeletal muscle cells were treated with increasing concentrations (0-50 ng/ml) of IGF-I in the absence or presence of $5{\mu}M$ SAHA for various time periods (3-24 hr). Treatment of C2C12 cells with IGF-I resulted in a dose- and time-dependent decrease in BDNF mRNA expression. However, inhibition of HDAC led to a significant increase in the expression of BDNF mRNA levels. In addition, immunocytochemistry revealed high BDNF protein levels in undifferentiated C2C12 skeletal muscle cells, whether untreated, IGF-I-treated, or exposed to SAHA. These results represent the first evidence that IGF-I can suppress the mRNA and protein expression of BDNF; conversely, SAHA attenuates the effects of IGF-I. Consequently, SAHA upregulates BDNF expression in C2C12 skeletal muscle cells.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.9
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• pp.2792-2799
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• 1996

It was shown in the previous study that the numerically derived elastic work factor for CLS specimen was independent of fiber direction for a unidirectional case. Also, it was proposed the elastic work factor could be used to determine energy release rate from a single test record. In the present study, elastic work factor was derived from a simple beam theory to investigate its dependence on material property and geometric condition. Also, the elastic work factor of CLS specimen was applied experimentally to determine critical energy release rate in order to prove its validity determining critical energy release rate from a single specimen. For this purpose, critical energy release rate determined using the elastic work factor was compared with that determined by the compliance method. The results showed that while elastic work factor is affected by $t_2/t_1$ and $L_2/L_1$ it is independent of fiber angle for a unidirectional case. It was also found that critical energy release rates determined by both methods are comparable each other, thus elastic work factor approach can be used to determine energy release rate from a single test specimen.

• Journal of the Korean Ceramic Society
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• v.23 no.3
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• pp.15-20
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• 1986

Formation of powders and electromechanical coupling factor of $Pb(Mn_{1/3} Sb_{2/3})_{0.08} Ti_{0.495}Zr_{0.425}O_3$ by hydro-thermal synthesis are described. The hydrothermal reactions each were accomplished at 12$0^{\circ}C$~25$0^{\circ}C$ for 5hours and sintering was accomplished at 1, .20$0^{\circ}C$ for 1hours. The PZT powders by hydrothermal synthesis were formed above 16$0^{\circ}C$ and the forms were cubic types. The ratio of grain size of sintered sample to powder was slowly decreased with the rising of hydrothermal reactino temperature but decreased rapidly above 22$0^{\circ}C$ Sintering density was decreased with the rising of hydrothermal reaction temperature above 16$0^{\circ}C$ but dielectric constant was increased. Electromechanical coupling factor $K_P$ was almost constant at 16$0^{\circ}C$~24$0^{\circ}C$ range and the value of $K_P$ was about 0.43-0.45.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.1250-1251
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• 2008

In this study, the dielectric ceramics with low loss were investigated for high frequency application. All sample of the $Ba_5M_4O_{15}$ (M=Ta, Nb) ceramics were prepared by the conventional mixed oxide method and sintered at $1325{\sim}1575^{\circ}C$. The bulk density and dielectric constant of the $Ba_5Ta_4O_{15}$ ceramics were increased continuously with increasing of sintering temperature. The quality factor was increased in the sintering temperature of $1375{\sim}1475^{\circ}C$ but decreased at the temperature above 1475$^{\circ}C$. In the case of $Ba_5Nb_4O_{15}$ ceramics, the bulk density, dielectric constant and quality factor were increased with sintering temperature but decreased above temperature of 1400$^{\circ}C$. The dielectric constant, quality factor and temperature coefficient of the resonant frequency (TCRF) of the $Ba_5Ta_4O_{15}$ and $Ba_5Nb_4O_{15}$ ceramics, sintered at 1475$^{\circ}C$ and 1400$^{\circ}C$, were 25.15, 53105 GHz, -3.06 ppm/$^{\circ}C$ and 39.55, 28052 GHz, 5.7 ppm/$^{\circ}C$, respectively.

• Molecules and Cells
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• v.28 no.5
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• pp.495-499
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• 2009

Although insulin-like growth factor-I (IGF-I) and androgen receptor (AR) are well known effectors of skeletal muscle, the molecular mechanism by which signaling pathways integrating AR and IGF-I in skeletal muscle cells has not been previously examined. In this study, the role of PI3K/Akt on IGF-I-induced gene expression and activation of AR in skeletal muscle cells was investigated. C2C12 cells were treated with IGF-I in the absence or presence of inhibitors of PI3K/Akt pathway (LY294002 and Wortmannin). Inhibition of the PI3K/Akt pathway with LY294002 or Wortmannin led to a significant decrease in IGF-I-induced AR phosphorylation and total AR protein expression. Furthermore, IGF-I-induced AR mRNA and skeletal ${\alpha}-actin$ mRNA were blocked by LY294002 or Wortmannin. Confocal images showed that IGF-I-induced AR translocation from cytosol to nucleus was inhibited significantly in response to treatment with LY294002 or Wortmannin. The present results suggest that modulating effect of IGF-I on AR gene expression and activation in C2C12 mouse skeletal muscle cells is mediated at least in part by the PI3K/Akt pathway.