• 대한화학회지
• /
• 제46권2호
• /
• pp.157-163
• /
• 2002

$tRNA^{Val}$에 결합하는 RNA 요소들을 확인하기 위해 SELEX 방법을 수행하였다. 양끝에 보존된 primer 서열을 가지고 가운데 무작위의 48-mer 올리고 누클레오티드 영역을 가진 DNA 문고를 T7 RNA 중합효소를 이용하여 전사시켜 얻은 RNA pool을 가지고 $tRNA^{Val}$이 고정된 affinity column을 이용하여 14번의 선별 과정을 거쳐 FNA aptamer들을 선별하였다. 몇몇 aptamer들은 세 가지 rRNA들의 고리 영역에 있는 서열과 유사한 서열을 가졌다: 5S rRNA의 C43GAAC47 서열, 16S rRNA의 G1491AAGU1495와 G1379UUCC1383 서열 그리고 23S rRNA의 C1064UUAG1068, G2110UGUA2114, C2480GACGG2485와 A2600CAGU2604 서열. 이 결과들은 $tRNA^{Val}$가 리보솜에서 5S rRNA, 16S rRNA 및 23S rRNA와 다양하게 상호작용 할 수 있다는 것을 암시한다.

• Bulletin of the Korean Chemical Society
• /
• 제23권8호
• /
• pp.1139-1143
• /
• 2002

Analysis of lysophosphatidic acids (LPAs) is of clinical importance as they can serve a potential marker for ovarian and other gynecological cancers and obesity. It is critically important to develop a highly sensitive and specific method for the early detection of gynecological cancers to improve the overall outcome of this disease. We have established a novel quantification method of LPAs in human plasma by negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring (MRM) mode without the conventional TLC step. Protein-bound lipids, LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as an internal standard under acidic condition. Following back extraction with chloroform and water, the centrifuged lower phase was evaporated and reconstituted in methanol. The reconstituted solution was directly injected into electrospray source of MS/MS. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:4 LPA, respectively. Q2 ions selected for MRM were m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01-25 ㎛olL-1 . The detection limit of the method was 0.3 pmol/mL, with a correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas of normal and gynecological cancer patients, this new method differentiated two different groups by way of total LPA level.

• The Korean Journal of Physiology
• /
• 제11권2호
• /
• pp.1-7
• /
• 1977

The activity of the $Ca^{++}$ activated adenosinetriphosphatase (Ca-ATPase) of actomyosin systeme of rabbit and frog skeletal muscle has been studied at varying pH and temperature. The PH optima of the Ca-ATPase activity of the rabbit actomyosin was rather broad. Over the temperature range of $16-36^{\circ}C$ activity of the enzyme was not appreciably changed between pH 6.4-8.5; below and above which it rapidly reduced. The pH at the inflection point of the enzyme activity increased as temperature decreased, showing the ${\bigtriangleup}pH\;inflection/{\bigtriangleup}T$ of approximately $-0.018\;unit/^{\circ}C$. Consequently, $(OH^-)/(H^+)$ ratio at the inflection point was constant regardless of assay temperature. In the frog actomyosin systems the Ca-ATPase activity was not apparently altered between PH 6.4-7.0 when the incubation temperature was $15{\sim}30^{\circ}C$. Outside of this range of pH, however, the enzyme activity was dramatically decreased. The pH of the inflection point changed inversely with temperature. ${\bigtriangleup}pH\;inflection/{\bigtriangleup}T$ at the acidic side was approximately $-0.018\;unit/^{\circ}C$, whereas that at the alkaline side it was about $-0.037\;unit/^{\circ}C$. The Arrhenius Plot on the Ca-ATPase activity at constant $(OH^-)/(H^+)$ ratio of 1.0 was not linear, but showed break at arround $20^{\circ}C$ for both rabbit and frog actomyosin Preparations. From these results it was speculated that pH dependence of Ca-ATPase activity of rabbit actomyosin systems might reflect titrations of histidine-imidazole and -SH groups, and that of the frog actomyosin represents titrations of histidine-imidazole and lysyllysine ${\alpha}-NH_2$ groups.

• 한국습지학회지
• /
• 제12권1호
• /
• pp.15-22
• /
• 2010

남한강과 낙동강의 버드나무군락에서 생태계 기능의 지표인 식물군집의 1차 순 생산량, 탄소흡수량과 낙엽분해율을 각각 측정하였다. 순 1차 생산량과 유기탄소흡수량은 각각 22.5ton/ha/yr(16.7ton/ha/yr-31.2ton/ha/yr), 9.7ton C/ha/yr(7.5ton C/ha/yr-14.0ton C/ha/yr)로서 최대값은 우리나라에서 보고된 값 중에서 가장 높았다. 이는 다른 군락보다 버드나무군락을 하천변에 조성하면, 이산화탄소를 빠르게 제거할 수 있음을 의미한다. 낙엽분해는 버드나무군락의 주변에서 가장 빠르게 분해가 일어났고, 버드나무군락 안, 초본군락 순으로 느리게 일어났다. 그 분해속도는 수생식물보다는 느리고, 육상식물보다는 빨랐다.

• 한국식품저장유통학회지
• /
• 제14권2호
• /
• pp.113-118
• /
• 2007

LED (light smiling diode)를 가정용 냉장고에 부착하여 양배추의 선도 유지에 미치는 영향을 조사하였다. 가정용 냉장고에 부착된 LED의 조사가 저장 중 양배추의 품질변화에 미치는 가장 큰 효과는 엽록소 합성, 비타민 C 및 페놀성분의 함량 증가였다. LED가 부착된 냉장고에 저장된 양배추는 절단면이 흰색에서 녹색으로 변화하였으며, 따라서 절단면의 Hunter a값이 일반 냉장고에 저장된 양배추에 비해 현저히 낮은 수치를 나타내었다. 일반 냉장고$(4^{\circ}C)$에 10일간 저장된 양배추에 비해 LED가 부착된 냉장고에 저장된 양배추의 엽록소 함량이 4-6배정도 높았으며, LED조사 시 저장 중 비타민 C함량은 약 2배정도, 페놀성분의 함량은 14%정도 증가시켜 양배추의 영양성분을 증가시키는 효과가 있었다.

• 대한간호학회지
• /
• 제49권1호
• /
• pp.59-68
• /
• 2019

Purpose: This study evaluated the psychometric properties of the Korean version of Yale Food Addiction Scale for Children (YFAS-C). Methods: Participants were 419 young adolescent students (11~15 years old). The content validity of the expert group was calculated as the content validity index (CVI) after the translation and reverse translation process of the 25 items of the YFAS-C. The multitrait-multimethod matrix (MTMM) method was used to verify the construct validity; the generalized linear model (GLM) was used to evaluate the concurrent and incremental validity. Reliability was calculated as Kuder-Richardson-20 (KR-20) and Spearman-Brown coefficients. Results: The CVI of the 25 items was greater than the item-level CVI .80 and the scale-level CVI .90. The Korean version of YFAS-C had verified convergent validity in emotional eating and external eating and discriminant validity in restrained eating. In addition, it had verified concurrent validity in emotional eating and external eating. Finally the incremental validity of the Korean version of YFAS-C was statistically significant on BMI. Reliability was KR-20 ${\alpha}=.69$ and the Spearman-Brown coefficient was .64. Conclusion: The Korean version of YFAS-C is a valid and reliable scale for measuring the severity of food addiction; it can be a useful scale for preventing obesity by predicting food addiction early.

• 한국식생활문화학회지
• /
• 제37권6호
• /
• pp.519-528
• /
• 2022

The Cheonggukjang used in this study was made with four soybean cultivars grown and harvested at the National Agrobiodiversity Center of the National Academy of Agricultural Sciences: C1 (KLS87248), C2 (Nongrim 51), C3 (GNU-2007-14613), and C4 (Daewon). The soybeans were inoculated with Bacillus subtilis AFY-2 and fermented for 0, 12, 21, 36, 45, and 60 h in a culture room at 35℃. ABTS and DPPH radical scavenging activities were significantly increased upon fermentation for 12 hours in all samples and maintained or decreased after 21 hour The inhibition of NO production was significantly increased compared to the LPS-treated group, and the C2 sample showed inhibition of NO production at 12 hours of fermentation, and the C3 sample showed inhibition at 21 hours of fermentation. PCA analysis revealed that the ABTS and DPPH radical scavenging activity and NO production inhibitory activity reached peak levels around 12 h fermentation time for all samples. Our results indicate that the optimal fermentation time of the Cheonggukjang sample is 12 hours, confirming the high correlation of all experiments.

• 한국자기공명학회논문지
• /
• 제26권1호
• /
• pp.10-16
• /
• 2022

The phase transition temperatures of CsCoCl₃·2H₂O crystals are investigated via differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Three endothermic peaks at temperatures of 370 K (=T_C1), 390 K (=T_C2), and 416 K (=T_C3) were observed for phase transitions from CsCoCl₃·2H₂O to CsCoCl₃·1.5H₂O, to CsCoCl₃·H₂O, and then to CsCoCl₃·0.5H₂O, respectively. In addition, the spin-lattice relaxation time T_1ρ in the rotating frame and T₁ in the laboratory frame as well as changes in chemical shifts for ¹H and ¹³³Cs near T_C1 were found to be temperature dependent. Our analyses results indicated that the changes of chemical shifts, T_1ρ, and T₁ are associated with structural phase transitions near temperature T_C1. The changes of chemical shifts, T_1ρ, and T₁ near T_C1 were associated with structural phase transitions, owing to the changes in the symmetry of the structure formed of H₂O and Cs⁺ ions. Consequently, the structural symmetry in CsCoCl₃·2H₂O crystals based on temperature is discussed by the environments of their H and Cs nuclei.

• 한국식품과학회지
• /
• 제14권2호
• /
• pp.122-124
• /
• 1982

한국산(韓國産) 개암 종실(種實)의 트리글리세리드의 조성(組成)을 HPLC에 의(依)해서 용매(溶媒)를 달리하여 분석(分析)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 즉, 개암 종실유(種實油)의 트리글리세리드는 탄소수 38부터 탄소수 48에 이르기까지 6종(種)의 트리글리세리드가 용매를 아세토니트릴-클로로포름-테트라히드로푸란(75 : 15 : 10, v/v/v)으로 사용(使用)할 때에 나타났으며, $C_{38}$이 4.14%, $C_{40}$ 5.23%, $C_{42}$가 10.03%, $C_{44}$가 24.02%, $C_{46}$이 48.73%이고, $C_{48}$이 7.85%였다. 그러나 동일(同一)한 조건(條件)이나 용매만을 바꾸어 아세토니트릴-테트라히드로푸란(70 : 30, v/v)을 사용(使用)하였을 때는 $C_{48}$은 전혀 나타나지 않았고, 다른 탄소수(炭素數) $C_{38}$부터 $C_{46}$까지는 모두 나타났으며, 그 함량(含量)은 $C_{38}$이 4.51%, $C_{40}$ 5.98%, $C_{42}$가 11.45%, $C_{44}$는 23.14%였으며, 여기에서도 $C_{46}$이 52.92%로서 그 함량(含量)이 가장 높았다. 따라서 개암 종실유(種實油) 중(中)에는 $C_{46}$에 해당하는 트리글러세리드의 함량(含量)이 주(主)를 이루고 그 다음으로 $C_{44}$에 해당하는 트리글리세리드가 약 25%로 높았으며, $C_{42}$의 약 11% 및 $C_{40}$의 $5{\sim}6%$, 그리고 $C_{38}$의 4%의 순(順)이었다.

• 한국식품과학회지
• /
• 제32권2호
• /
• pp.252-257
• /
• 2000

국내에서 재배된 아마란스 전분의 활용능력을 평가하기 위하여 다섯 품종의 아마란스(Andy, Suvarna, Nu World, K266-1, K432)로부터 전분을 분리하여 이화학 특성과 호화 특성을 조사하였다. 전분 입자의 모양은 모두 다면체였고 입자의 직경은 $1.14{\sim}1.48\;{\mu}m$였다. X-선 회절도에 의한 아마란스 전분의 결정형은 전형적인 A형임을 알 수 있었다. 전분의 단백질과 조지방질, 총지방질 함량은 각각 $0.13{\sim}0.23%$, $0.01{\sim}0.05%$, $0.05{\sim}0.10%$였고 아밀로오스 함량은 $2.79{\sim}4.35%$였으며 물 결합능력은 $128.05{\sim}135.80%$였다. 0.1% 농도로 측정한 아마란스 전분의 광투과도는 K266-1는 $60^{\circ}C$, 나머지는 $65^{\circ}C$에서 급격하게 증가하여 $70^{\circ}C$ 이후에는 완만한 증가를 보였다. 신속점도측정계로 측정한 전분의 호화 온도와 피크 온도가 각각 $71.3{\sim}73.7^{\circ}C$, $81.5{\sim}84.0^{\circ}C$였으며 피크 점도와 냉각 점도는 Nu World>Andy>Suvarna>K432>K266-1순이었다. 다섯 품종 중 Nu World는 가장 높은 피크 점도, 냉각점도를 보였고 K266-1는 가장 낮은 setback(-38) 값을 보였다. 시차주사열량기에 의한 아마란스 전분의 호화개시온도는 $65.7{\sim}68.0^{\circ}C$였고 피크온도는 $70.6{\sim}75.8^{\circ}C$였으며 엔탈피는 K266-1