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Analysis of Expressed Sequence Tags of the Spider, Araneus ventricosus

  • Chung, Eun-Hwa;Lee, Kwang-Sik;Han, Ji-Hee;Sohn, Hung-Dae;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.3 no.2
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    • pp.191-199
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    • 2001
  • We have constructed a cDNA library from the whole body of the spider, Araneus ventricosus. Sequence analysis of randomly selected cDNA clones was performed to obtain genetic information on the spider A. ventricosus, of which genetic information is currently not available. We have partially sequenced randomly selected 385 clones of the cDNA library constructed from A. ventricosus. This expressed sequence tags (EST) analysis revealed 383 genes had high homologies to known genes in GenBank. In this report, 241 independent genes were analyzed in detail.

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Nucleotide Sequence of ${\beta}-tubulin$ Gene from the Soft Coral Scleronephthya gracillimum $(K\ddot{u}kenthal)$

  • Yum, Seung-Shic;Woo, Seon-Ock;Chang, Man;Lee, Taek-Kyun;Song, Jun-Im
    • Ocean Science Journal
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    • v.40 no.1
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    • pp.55-59
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    • 2005
  • We cloned the complete cDNA of the ${\beta}-bubulin$ from the soft coral, Scleronephthya gracillimum $(K\ddot{u}kenthal)$ (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5'-rapid amplification of cDNA end (RACE) technique. The full-length cDNA of the S. gracillimum ${\beta}-tubulin$ comprised 1541 bp, not including the poly $A^+$ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues 16402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that the sequence we have obtained is, indeed, the full-length cDNA of the ${\beta}-bubulin$ gene in S. gracillimum.

Identification of the Differentially Expressed Genes of Hanwoo During the Growth Stage by Subtractive cDNA Hybridization (Subtraction 기법을 이용한 한우 성장 단계 특이 발현 유전자 탐색)

  • Jang, Y.S.;Kim, T.H.;Yoon, D.H.;Park, E.W.;Cheong, I.C.;Jo, J.K.
    • Journal of Animal Science and Technology
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    • v.44 no.1
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    • pp.13-22
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    • 2002
  • To identify the differentially expressed genes at growth stage of Hanwoo, we constructed the subtractive cDNA library from loin mRNA of 12- and 24-month old Hanwoo by PCR-based subtraction. The fourteen genes were confirmed by sequencing and reverse northern blot analysis, and they were selected as candidate of putative genes differentially expressed at the growth stage of Hanwoo. Three subtracted cDNA fragments that expressed specific signal to cDNA probe for 6-month-old loin of Hanwoo were highly homologous to those of the genes encoding EPV 20, Ca2+ATPase, and TCTP, respectively. The nine cDNA clones showed intense signal to cDNA probe from 12-month-old loin of Hanwoo, and highly homologus to those of genes encoding VCP, HSP 70, aldolase A, MSSK1, GM-2 activator protein, ryanodine receptor, acidic ribosomal phosphoprotein p1, ADP/ATP translocase, and UCP 2, respectively. Two subtracted cDNA clones that expressed specific signal to cDNA probes for 12- and 24-month-old loin of Hanwoo were detected. One of them was highly homologus to the gene encoding ferrochelatase and the other was highly homologus to the gene encoding ADRP.

The Mobility and Career Pathes of Alumni of Whose Major in Library and Information Sciences in Korea (문헌정보학 전공자의 경력개발 경로를 통한 유동성 실태연구)

  • Ahn, In-Ja
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.21 no.4
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    • pp.133-147
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    • 2010
  • With the start of knowledge based society, there has been extinction of traditional librarian jobs, new kinds of jobs are created, differentiated, and joined. As so there has been a great change in career paths. This study is a basic research of career parhes of library and information sciences alumni to adapt field work and build powerful workforce. Through the research of 5 Universities and 190 career routes, the movement of the library information and science majors' career has been unveiled. The majority of the library and information science majored career was jobs based on culture and arts, education and researches, economy and planning related ones, marketing and sales respectively. Particularly, except the librarian, there were just few careers in artistic cultural field. Further more, because there is a great impact in career tendency based on his education, the library information and science has drawn the report that the future of career and knowledge which is demanded in working field.

Usage of Social Media Tools by Library and Information Professionals (LIPs) in selected Academic Libraries in South-West, Nigeria

  • Nduka, Stella C.;Adekanye, Elizabeth A.;Adedokun, Titilayo O.
    • International Journal of Knowledge Content Development & Technology
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    • v.11 no.3
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    • pp.7-27
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    • 2021
  • This study was carried out to examine the awareness and use of social media tools by library and information professionals (LIPs) in selected academic libraries in South-west, Nigeria and the challenges they face in the use of social media technologies. Descriptive survey was adopted for this study. The population of the study comprised 217 library and information professionals from 10 selected academic libraries in south-west, Nigeria. A total enumeration technique was used to cover all the library and information professionals, 136 respondents filled and returned the questionnaire, given a response rate of 62.7%. The questionnaire was used as instrument for data collection. Descriptive statistics was used to analyze the data collected. The findings show that majority of LIPs possessed a high level of awareness in the use of social media tools. The study also revealed that social network tools were highly used by LIPs in the academic libraries studied and the types of social media used by LIPs was also revealed. The major challenges faced in the use of social media include inadequate power supply, lack of Internet access and time constraints. The paper recommended that to enhance the use of social media by LIPs, there is need for constant awareness of the importance of social media tools to LIPs and libraries in effective service delivery, LIPs should be ready to learn, unlearn and be learned in the use of social media and university libraries should provide enabling environment such as internet connectivity, power supply and policy to guide LIPs in social media usage.

Immune Gene Discovery by Expressed Sequence Tags Generated from Olive Flounder (Paralichthys olivaceus) Kidney (넙치 (Paralichthys olivaceus) 신장에서 생성된 ESTs (Expressed Sequence Tags)로부터 면역관련 유전자의 탐색)

  • Lee, Jeong-Ho;Kim, Young-Ok;Kim, Jong-Hyun;Noh, Jae Koo;Kim, Hyun Chul;Kim, Kyung-Kil;Kim, Kyu-Won
    • Korean Journal of Ichthyology
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    • v.18 no.4
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    • pp.283-292
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    • 2006
  • Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the kidney mRNA of olive flounder (Paralichthys olivaceus). In the survey of 390 ESTs chosen from the kidney cDNA library, 250 ESTs showed significant homology to previously described genes while 140 ESTs were unidentified or novel. Comparative analysis of the 250 identified ESTs showed that 14 (5.6%) clones were representing 11 unique genes identified as homologous to the previously reported olive flounder ESTs, 198 (79.2%) clones representing 160 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 38 (15.2%) clones representing 37 genes of known sequences with unknown functions. We also identified several kinds of immune associated proteins, indicating EST as a powerful method for identifying immunerelated genes of fish as well as identifying novel genes. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

A study on content structure of literature search methodology (문헌조사법의 내용구조에 관한 고찰)

  • 김정소;박준식
    • Journal of Korean Library and Information Science Society
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    • v.10
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    • pp.27-58
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    • 1983
  • The purpose of this study is to clarify the educational literature search methodology objectives and the content structure of a course in bibliography. The content of this study can be summarized as follows: 1. The concept and domain of literature search methodology, which has traditionally gone by the name of a guide to use of literature and library, are described. 2. The need of incorporating the methodology into regular college curriculum was investigated in view of the ideology of college education. 3. The background, process, and current status of offering a course in the methodology as a part of regular college curriculum were examined. 4. The educational objective of a course in the methodology was set at the successful activities in self study, life-long-education, and special topic research. 5. Considering the fact that technical knowledge and propositional knowledge should be realized simultaneously, that educational experience should go hand in hand with materials and library, and that knowledge should be constructed systematically and logically, the content structure of the methodology were divided into the following six categories: a) the significance of the methodology of literature search b) the college library and the activities of literature search c) types and characteristics of materials d) literature search by catalog e) explanation of basic bibliography f) research and literature search

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Economic Efficiency of Digital Library : A Case Study on the DLP of the National Digital Library (디지털도서관의 경제성 평가 : 국립디지털도서관 DLP 사례 연구)

  • Kim, Dong-Seok;Jo, Dong-Ho;Ko, Young-Man
    • Journal of the Korean Society for information Management
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    • v.20 no.4 s.50
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    • pp.159-193
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    • 2003
  • This study is a case study that attempt to quantify the economic efficiency of 'digital library program(DLP) of the National Library of Korea' by means of cost-benifit analysis. Results indicate that the B/C ratio is 1.70 which can be interpreted the economic efficiency of DLP is very high. Of particular significance is the fact that this study empirically proves that the digital library program is economically efficient.

Toward iSchools: from the Perspective of the 5Cs

  • Yi, Myongho
    • Journal of the Korean Society for Library and Information Science
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    • v.50 no.1
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    • pp.313-330
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    • 2016
  • A coalition of information schools, called the iSchools Organization, was established to increase the visibility of library and information science and to cope with the new demands of the digital age. As of 2015, sixty-five schools from many different countries have joined the iSchools Organization. While some other schools are interested in adopting iSchools, there are still some issues that need to be considered before adopting the iSchool charter. This paper presents those issues from the perspectives of the 5C groups: curriculum, competencies, convergence, collaboration, and consensus. A survey was conducted to investigate some aspects of the 5Cs. This study identifies five iSchool-related issues - 5Cs. Providing perspectives in the areas of the 5Cs will be useful to establish stronger iSchools. These five Cs will resolve information problems that we are facing, prepare students or any organizations for the digital age, give students digital service skills, and train future data scientists. This paper represents practical guidelines to build a strong iSchool. With the success of iSchools, societies see us as more than the traditional librarian.