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http://dx.doi.org/10.5187/JAST.2002.44.1.013

Identification of the Differentially Expressed Genes of Hanwoo During the Growth Stage by Subtractive cDNA Hybridization  

Jang, Y.S. (National Livestock Research Institute, RDA)
Kim, T.H. (National Livestock Research Institute, RDA)
Yoon, D.H. (National Livestock Research Institute, RDA)
Park, E.W. (National Livestock Research Institute, RDA)
Cheong, I.C. (National Livestock Research Institute, RDA)
Jo, J.K. (Department of animal science, Kyungpook National University)
Publication Information
Journal of Animal Science and Technology / v.44, no.1, 2002 , pp. 13-22 More about this Journal
Abstract
To identify the differentially expressed genes at growth stage of Hanwoo, we constructed the subtractive cDNA library from loin mRNA of 12- and 24-month old Hanwoo by PCR-based subtraction. The fourteen genes were confirmed by sequencing and reverse northern blot analysis, and they were selected as candidate of putative genes differentially expressed at the growth stage of Hanwoo. Three subtracted cDNA fragments that expressed specific signal to cDNA probe for 6-month-old loin of Hanwoo were highly homologous to those of the genes encoding EPV 20, Ca2＋ATPase, and TCTP, respectively. The nine cDNA clones showed intense signal to cDNA probe from 12-month-old loin of Hanwoo, and highly homologus to those of genes encoding VCP, HSP 70, aldolase A, MSSK1, GM-2 activator protein, ryanodine receptor, acidic ribosomal phosphoprotein p1, ADP/ATP translocase, and UCP 2, respectively. Two subtracted cDNA clones that expressed specific signal to cDNA probes for 12- and 24-month-old loin of Hanwoo were detected. One of them was highly homologus to the gene encoding ferrochelatase and the other was highly homologus to the gene encoding ADRP.
Keywords
Hanwoo; Growth stage; Differentially expressed gene; Subtraction;
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