• Korean Journal of Medicinal Crop Science
• /
• v.23 no.5
• /
• pp.385-394
• /
• 2015

Background : Dibenzocyclooctadiene lignans are secondary metabolites present abundantly in the fruits belonging to the genus Schisandra. According to previous studies, Schisandra lignans exhibit anti-inflammatory, anti-cancer and anti-diabetic properties, as well as an inhibitory effect on platelet aggregation. Therefore, establishing the Korean "Omija" (Schisandra chinensis) as a lignan-rich source, in addition to identifying and quantifying the lignans, is extremely valuable. Methods and Results : Dibenzocyclooctadiene lignans were analyzed with liquid chromatography using diode array detection/mass spectrometry, from methanol extracts subsequently identified by a constructed chemical library of 50 lignans. A total of 27 components of lignan including gomisin S were identified, of which schisandrin, gomisin A, gomisin N, deoxyschisandrin, ${\gamma}$-schisandrin, and schisandrin C were identified as the major components in the Korean Omija, Schisandra chinensis. These compounds were divided into two groups, S-biphenyl and R-biphenyl based on the configurations of the stereoisomers structures with contents of 661.7 and 1350.1mg per 100 g dry weight, respectively. The total lignan content averaged 2011.4mg per 100 g dry weight, of which schisandrin and gomisin N comprised the majority (771.8 and 420.5mg per 100 g dry weight respectively). Conclusions : Lignans which are present in high quantities in the ripe fruit of Schisandra chinensis are important functional compounds that play a major role in the prevention and treatment of human diseases.

• Korean Journal of Microbiology
• /
• v.29 no.1
• /
• pp.16-24
• /
• 1991

The yeast gene THR1 encodes the homoserine kinase (EC 2.7.1.39: HKase) which catalyses the first step of the threonine specific arm at the end of the common pathway for methionine and threonine biosynthesis. A recombinant plasmid pMC3 (12.6 kilobase pairs, vector YCp50) has been cloned into E. coli HB101 from a yeast genomic library through its complementing activity of a thr1 mutation in a yeast recipient strain M39-1D. When subcloned into pMC32 (8.6kbp, vector YRp7) and pMC35 (8.3 kbp, vector YIp5), the HindIII fragment (2.7 kbp) of pMC3 insery was positive in the thrI complementing activity in both yeast and E. coli auxotrophic strains. The linearized pMC35 was introduced into the original recipient yeast strain and the mitotically stable chromosomal integrant was identified among the transformants. Through the tetrad analysis, the integration site of the pMC35 was localized to the region of THR1 structural gene at an expected genetic distance of approximately 11.1 cM from the ARG4 locus on the right arm of the yeast chromosome VIII. When episomically introduced into the auxotrophic cells and cultured in Thr omission liquid medium, the cloned gene overexpressed the HKase in the order of thirteen to fifteenfold, as compared with a wildtype. HKase levels are repressed by addition of threonine at the amount of 300 mg/l and 1, 190 mg/l for pMC32 and pMC3, respectively. Data from genetic analysis and HKase response thus support that the cloned HindIII yeast DNA fragment contains the yeast thr1 structural gene, along with necessary regulatory components for control of its proper expression.

• Microbiology and Biotechnology Letters
• /
• v.35 no.3
• /
• pp.177-183
• /
• 2007

Using complementation of RpoH deficient E. coli strain A7448, the rpoH gene encoding heat shock sigma factor 32 (${\sigma}^{32}$) from Methylovorus sp. strain SS1 DSM11726 was cloned and sequenced. Sequence analysis of a stretch of 1,796-bp revealed existence of an open reading frame encoding a polypeptide of 284 amino acid (32,006 dalton). Deduced amino acid sequence of the Methylovorus sp. strain SS1 RpoH showed that 59.6%, 39.1% and 51.4% identities with those of Nitrosomonas europaea (${\beta}$-proteobacteria), Agrobacterium tumefaciens ($\alpha$-proteobacteria) and E. coli (${\gamma}$-proteobacteria). The expression level of the functional ortholog of RpoH of Methylovorus sp. strain SS1 was increased transiently after heat induction, further indicating that it functions as a heat shock sigma factor.

• Journal of Korean Biological Nursing Science
• /
• v.22 no.1
• /
• pp.1-10
• /
• 2020

Purpose: Diabetes Mellitus (DM) is a leading cause of death with a prevalence rate of 12.4% in South Korea. Self-management is crucial for patients with DM, because many studies have reported that self-management intervention based on the Health Belief Model (HBM) is effective. The purpose of this study was to investigate the current state of HBM based intervention studies and the components and effects of the theories used in the study for diabetes patients. Methods: A systematic review was conducted using the Pubmed, Cochrane Library and Embase databases from January 2009 to May 2019. We reviewed characteristics of intervention based on the HBM in randomized controlled clinical trials (RCTs), quasi-experimental study intervention. Results: Eight studies published in English between 2009 and 2019 were included in this review. The key components of the health behavior promotion program applied to the DM patients were perceived susceptibility, perceived severity, perceived benefits, perceived barriers and self-efficacy. The intervention based on these components has reported to significantly increase the health behavior change, likelihood of taking health action and improve physiological indicators (HbA1c, and fasting blood sugar etc.). Conclusion: This study highlighted the importance of intervention programs based on the HBM for DM patients.

• Journal of Korean Academy of Nursing
• /
• v.50 no.4
• /
• pp.513-522
• /
• 2020

Purpose: This study aimed to utilize concept analysis to obtain a better understanding of the concept of "continuity of care" in chronic diseases. Methods: The concept of continuity of care was analyzed using the Walker and Avant method. Covering literature in English from 1930 to 2018, the data sources included CINAHL Complete, Academic Search Complete, MEDLINE, PsyARTICLES, Health Source: Nursing/Academic Edition, Google Scholar, Science Direct, and the Cochrane Library. Results: A comprehensive definition of concept of continuity of care was developed based on a systematic search and synthesis. The key defining attributes were identified as (a) care over time, (b) the relationship between an individual patient and a care team, (c) information transfer, (d) coordination, and (e) meeting changing needs. The antecedents of continuity of care were having a chronic disease, inexperienced with disease management, a poorly coordinated healthcare system, and medical care limitations. The consequences of continuity of care were decreasing hospital admissions, reducing costs, reducing emergency room visits, improving the quality of life, improving patient satisfaction, and delivering good healthcare. Conclusion: The thorough concept analysis provides insight into the nature of "continuity of care" in chronic diseases and also helps ground the concept in healthcare.

• Journal of the Korean Home Economics Association
• /
• v.26 no.3
• /
• pp.135-152
• /
• 1988

The purpose of this study was to survey the improvement activities for the apartment house ; and to examine the relationship between apartment residents and their housing improvement activities in order to present some informations for the further studies and plans of apartment design and housing policy. Library and questionnaire survey methods were used in this research. the sample was taken from 102 housewives living in the apartments over 45 pyong (1602 sq. ft.), in Seoul, Data were analyzed with the SPSS using frequency, percentage, chi-square test, F-test, Duncan's multiple range test, η2, and C2. The results were as follows: 1. It was found that residents had experienced with 3.6 kinds of housing improvement activities. Living rooms, dining rooms, and kitchens in family living areas ; balconies and utility rooms in utility areas were improved for the physical convenience and quality of living. 2. This survey indicated that apartment residents evaluated thier housing condition as high, except storage spaces and outdoor noises. 3. About 80% of respondents were expected to live continuously in the current apartments because they were satisfied with the size of thier residences, However, they wanted to improve the inside of the entire apartment, especially bathrooms. 4. Variables which had a significant relationship in housing improvement activities in the past were the length of residence, the expected length of residence, and the characteristic of residence. 5. The expected length of residence, and the characteristic of residence were found to be effective variable for evaluating the current residence. 6. Finally, the expected length of residence, and the characteristic of residence were significant variables of the housing plan in the future. Housing improvement activities were prevalent recently and expected to be continued in the future. It can be said, therefore, that apartment residents have a long -term plan to live in one place rather than moving out ever two-three years.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.30 no.12C
• /
• pp.1193-1200
• /
• 2005

In this paper, we proposed an image hiding method which decreases the amount of calculation encrypting partial data rather than the whole image data using a discrete wavelet transform and a linear scalar quantization which have been adopted as the main technique in JPEG2000 standard and then implemented the proposed algorithm to hardware. A chaotic system was used instead of encryption algorithms to reduce further amount of calculation. It uses a method of random changing method using the chaotic system of the data in a selected subband. For ciphering the quantization index it uses a novel image encryption algorithm of cyclical shifting to the right or left direction and encrypts two quantization assignment method (Top-down coding and Reflection coding), made change of data less. The experiments have been performed with the proposed methods implemented in software for about 500 images. The hardware encryption system was synthesized to find the gate-level circuit with the Samsung $0.35{\mu}m$ Phantom-cell library and timing simulation was performed, which resulted in the stable operation in the frequency above 100MHz.

• BMB Reports
• /
• v.53 no.6
• /
• pp.335-340
• /
• 2020

Since cancer is the leading cause of death worldwide, there is an urgent need to understand the mechanisms underlying cancer progression and the development of cancer inhibitors. Signal transducer and activator of transcription 3 (STAT3) is a major transcription factor that regulates the proliferation and survival of various cancer cells. Here, dual-specificity phosphatase 3 (DUSP3) was identified as a regulator of STAT3 based on an interaction screening performed using the protein tyrosine phosphatase library. DUSP3 interacted with the C-terminal domain of STAT3 and dephosphorylated p-Y705 of STAT3. In vitro dephosphorylation assay revealed that DUSP3 directly dephosphorylated p-STAT3. The suppressive effects of DUSP3 on STAT3 were evaluated by a decreased STAT3-specific promoter activity, which in turn reduced the expression of the downstream target genes of STAT3. In summary, DUSP3 downregulated the transcriptional activity of STAT3 via dephosphorylation at Y705 and also suppressed the migratory activity of cancer cells. This study demonstrated that DUSP3 inhibits interleukin 6 (IL-6)/STAT3 signaling and is expected to regulate cancer development. Novel functions of DUSP3 discovered in IL-6/STAT3 signaling regulation would help expand the understanding of cancer development mechanisms.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.27 no.10C
• /
• pp.963-972
• /
• 2002

An 8192-point pipelined FFT/IFFT processor core is designed, which can be used in multi-carrier modulation systems such as DUf-based VDSL modem and OFDM-based DVB system. In order to improve the signal-to-quantization-noise ratio (SQNR) of FFT/IFFT results, two-step convergent block floating-point (TS_CBFP) scaling is employed. Since the proposed TS_CBFP scaling does not require additional buffer memory, it reduces memory as much as about 80% when compared with conventional CBFP methods, resulting in area-and power-efficient implementation. The SQNR of about 60-㏈ is achieved with 10-bit input, 14-bit internal data and twiddle factors, and 16-bit output. The core synthesized using 0.25-$\mu\textrm{m}$ CMOS library has about 76,300 gates, 390K bits RAM, and twiddle factor ROM of 39K bits. Simulation results show that it can safely operate up to 50-㎒ clock frequency at 2.5-V supply, resulting that a 8192-point FFT/IFFT can be computed every 164-${\mu}\textrm{s}$. It was verified by Xilinx FPGA implementation.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.27 no.6C
• /
• pp.601-609
• /
• 2002

The efficient Viterbi decoder that supports full data-rate output of DAB system was proposed. Viterbi decoder consumes lots of computational load and should be designed to be fast specific hardware. In this paper, SST scheme was adopted for Viterbi decoder with puncturing to reduced the power consumption. Puncturing vector tables are modified and re-arranged to be designed by a hardwired logic to save the system area. New re-scaling scheme which uses the fact that the difference of the maximum and minimum of the path metric values is bounded is proposed. The proposed re-scaling scheme optimizes the wordlength of path metric memory and greatly reduces the computational load for re-scaling by controlling MSB of path metric memory. Another saving of computation is done by proposed algorithm for branch metric calculation, which makes use of pre-calculated metric values. The designed Viterbi decoder was synthesized using SAMSUNG 0.35$\mu$ standard cell library and occupied small area and showed lower power consumption.