• Title/Summary/Keyword: C/P

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Effect of Green Tea on Tissue Distribution and Deposition of 14C-Benzo[a]pyrene in Rats (흰쥐에서 녹차의 섭취가 14C-Benzo[a]pyrene의 조직 분배 및 잔류에 미치는 영향)

  • Kim, Ju-Yeon;Noh, Sang-K.
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.6
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    • pp.818-823
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    • 2011
  • Recently, we showed that green tea extract (GTE) markedly lowers the intestinal absorption of $^{14}C$-benzo[a]pyrene ($^{14}C$-BaP) and enhances its secretion into the biliary route, suggesting a protective role for GTE against body burden. These findings indicate that green tea could be used as an effective dietary means against the toxicity of BaP. The present study, therefore, was designed to investigate if green tea intake could affect the tissue distribution and deposition of $^{14}C$-BaP in rats. Male Sprague-Dawley rats had free access to a nutritionally adequate AIN-93G diet and deionized water. At ~340 g of weight, the rats were injected intraperitoneally with 27.4 kBq of [4-$^{14}C$]-BaP and 5.0 mg of BaP dissolved in $300\;{\mu}L$ of olive oil and then assigned randomly to the following two groups: one group (GTE) of rats was fed the AIN-93G diet with GTE via drinking water at approx. 4.7 mg of catechins/d, whereas the other was fed the same diet but without GTE (control). At 4 wk of dietary treatment with GTE, animals were euthanized and heart, liver, brain, spleen, kidney, retroperitoneal fat, testis, and epididymal fat were collected, weighed, and analyzed for tissue $^{14}C$-BaP. Both the control and GTE groups continuously gained weight throughout the study, but there was no significant difference between the groups. No significant differences were observed in the weights of heart, liver, brain, spleen, kidney, retroperitoneal fat, testis, and epididymal fat. However, the radioactivities of $^{14}C$-BaP, expressed in dpm/g, were significantly lower in the heart, liver, brain, spleen, and epididymal fat of rats receiving GTE as compared to their respective controls. These data indicate that green tea intake markedly lowers tissue accumulation of $^{14}C$-BaP. Taken together, these findings suggest that the decreased tissue levels of BaP by GTE intake may be associated with lowered intestinal absorption of BaP and its enhanced secretion into the bile.

Selection of Isochrysis and Pavlova Species for Mass Culture in High Temperature Season (고온기 배양에 적합한 Isochrysis와 Pavlova 종의 선정)

  • Yang, Sung-Jin;Hur, Sung-Bum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.4
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    • pp.343-350
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    • 2012
  • Even though the microalgal species of Isochrysis and Pavlova are widely used as live food in bivalve hatcheries, they are difficult to culture in mass during the summer season. Therefore, the present study was conducted to determine the optimum species or strains of Isochrysis and Pavlova to produce good growth and high contents of fatty acids at temperatures over $30^{\circ}C$. Four species of Isochrysis (I. galbana KMMCC12, I. galbana KMMCC214, I. aff. galbana, and Isochrysis sp.) and four of Pavlova (P. lutheri, P. gyrans, P. viridis, and Pavlova sp.) were cultured at $25^{\circ}C$, $29^{\circ}C$, and $33^{\circ}C$, and then analyzed for specific growth rate and fatty acid composition. Microalgae were cultured in f/2 medium at 23 psu and continuous light of $80{\mu}mol$ photons $m^{-2}s^{-1}$. For the I. galbana, growth rates were highest at $29^{\circ}C$ and decreased at $33^{\circ}C$ to the level observed at $25^{\circ}C$. I. galbana (KMMCC12) and Isochrysis sp. cultured at $29^{\circ}C$ and $33^{\circ}C$, respectively, exhibited the highest growth rates of all Isochrysis species. In terms of fatty acids, I. galbana (KMMCC12) contained higher contents of PUFA and n-3 HUFA at $33^{\circ}C$ than did Isochrysis sp. For species of Pavlova, growth rates of P. gyrans and P. viridis at $29^{\circ}C$ and $33^{\circ}C$, respectively, were higher than those of the other Pavlova species. In particular, P. viridis grew as well at $33^{\circ}C$ as it did at $29^{\circ}C$. However, P. lutheri and Pavlova sp. did not grow at $33^{\circ}C$. In terms of fatty acids, P. viridis cultured at $33^{\circ}C$ also exhibited higher contents of PUFA and n-3 HUFA, as compared to P. gyrans. Based on these results, we suggest that I. galbana (KMMCC12) and P. viridis are suitable species for mass culture during the high temperature season.

Optimum Culture Condition on Four Species of Microalgae used as Live Food for Seedling Production of Bivalve (이매패류 먹이생물로 이용되는 미세조류 4종의 적정 배양환경조건)

  • Min, Byeong-Hee;Hur, Sung Bum
    • The Korean Journal of Malacology
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    • v.31 no.1
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    • pp.35-41
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    • 2015
  • In order to investigate the live food value of microalgae for efficacious rearing of larvae and spats of bivalve, we studied growth rates of four microalgal species (Isochrysis galbana, Pavlova lutheri, Chaetoceros simplex, Tetraselmis tetrathele) cultured in different environmental conditions. These include changes in temperatures (20, 25, 30 and $35^{\circ}C$), salinities (20, 25, 30 and 33 psu) and light intensities (60, 100 and $140{\mu}mol\;m^{-2}s^{-1}$). The growth rate of I. galbana was faster at $25^{\circ}C$ than that of $20^{\circ}C$. At $25^{\circ}C$ the highest growth rate of I. galbana was observed at 33 psu (0.413) and the lowest at 20 psu (0.368) in 10 days of culture (P < 0.05). The growth rate of I. galbana was lower at 25 psu (0.383) than that of 30 psu and higher than that of 20 psu (P < 0.05). Similar temperature and salinity-dependent changes were also found in P. lutheri and T. tetrathele. C. simplex showed faster growth rate at $30^{\circ}C$ than that of $25^{\circ}C$. The highest growth rate of C. simplex was observed at 33 psu (0.428) and the lowest at 20 psu (0.389) in 10 days of culture (P < 0.05). Upon exposure to the light with different intensities, all four microalgal species showed a significantly faster growth rate at $140{\mu}mol\;m^{-2}s^{-1}$ than at $100{\mu}mol\;m^{-2}s^{-1}$ (P < 0.05).

Effect of different short-term high ambient temperature on chicken meat quality and ultra-structure

  • Zhang, Minghao;Zhu, Lixian;Zhang, Yimin;Mao, Yanwei;Zhang, Mingyue;Dong, Pengcheng;Niu, Lebao;Luo, Xin;Liang, Rongrong
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.5
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    • pp.701-710
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    • 2019
  • Objective: This study investigated the effect of different acute heat stress (HS) levels on chicken meat quality and ultra-structure. Methods: Chickens were randomly divided into 7 groups to receive different HS treatments: i) $36^{\circ}C$ for 1 h, ii) $36^{\circ}C$ for 2 h, iii) $38^{\circ}C$ for 1 h, iv) $38^{\circ}C$ for 2 h, v) $40^{\circ}C$ for 1 h, vi) $40^{\circ}C$ for 2 h, and vii) un-stressed control group ($25^{\circ}C$). Blood cortisol level, breasts initial temperature, color, pH, water holding capacity (WHC), protein solubility and ultra-structure were analyzed. Results: HS temperatures had significant effects on breast meat temperature, lightness ($L^*$), redness ($a^*$), cooking loss and protein solubility (p<0.05). The HS at $36^{\circ}C$ increased $L^*{_{24h}}$ value (p<0.01) and increased the cooking loss (p<0.05), but decreased $a^*{_{24h}}$ value (p<0.05). However, as the temperature increased to $38^{\circ}C$ and $40^{\circ}C$, all the values of $L^*{_{24h}}$, cooking loss and protein denaturation level decreased, and the differences disappeared compared to control group (p>0.05). Only the ultimate $pH_{24h}$ at $40^{\circ}C$ decreased compared to the control group (p<0.01). The pH in $36^{\circ}C$ group declined greater than other heat-stressed group in the first hour postmortem, which contributed breast muscle protein degeneration combining with high body temperature, and these variations reflected on poor meat quality parameters. The muscle fiber integrity level in group $40^{\circ}C$ was much better than those in $36^{\circ}C$ with the denatured position mainly focused on the interval of muscle fibers which probably contributes WHC and light reflection. Conclusion: HS at higher temperature (above $38^{\circ}C$) before slaughter did not always lead to more pale and lower WHC breast meat. Breast meat quality parameters had a regression trend as HS temperature raised from $36^{\circ}C$. The interval of muscle fibers at 24 h postmortem and greater pH decline rate with high body temperature in early postmortem period could be a reasonable explanation for the variation of meat quality parameters.

대 잠수함초계기 P-3C - 그 내력과 원리를 알아본다

  • Seo, Byeong-Hong
    • Aerospace Industry
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    • v.30
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    • pp.77-80
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    • 1995
  • 우리 해군이 지난 4월말 대잠수함 초계비행기인 P-3C를 도입했다는 기사가 보도 되었다. 이 P-3C 기종은 록키드사가 만든 세계에서 가장 우수한 잠수함 초계 및 격침용으로 정찰, 초계, 공격을 모두할 수 있는 항공기로 알려져 있다. 이에 여기서는 P-3C기가 개발된 배경과 잠수함 킬러로서의 구조적 특징과 원리에 대해서 알아본다.

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Variation of Vital Sign according to Time in Full Immersion of Hot and Cool Bath (온.냉욕 전신침수욕시 기간에 따른 vital sign의 변화)

  • Yi, Seung-Ju
    • Journal of Korean Physical Therapy Science
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    • v.3 no.3
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    • pp.35-49
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    • 1996
  • This study was conducted to see variation of vital sign of hot and cool bath according to time, a questionnair survey and measurement was carried out for 32 students(sophomore) of department of physical therapy Andong Junior College on the 27th of June, 1995. The result were as follows: The average systolic blood pressure(SBP) of stability for 32 college students who were measured was 105.3mmHg, the average diastolic blood pressure(DBP) was 67.3mmHg, the average pulse frequency(PF) was 70.7(frequency/min), the average respiratory frequency (RF) was 15.6 (frequency/min), and the body temperature(BT) was $36.6^{\circ}C$. As time went on, SBP for 32 students who were measured in hot bath according to stability, 3 min, 6 min, 9 min, and 12 min was decreased(105.15 mmHg, 104.69mmHg, 104.24 mmHg, 103.03 mmHg, and 96.69 mmHg)(P=0.3006). SBP was decreased in cool bath, too(105.15 mmHg, 103.33 mmHg, 103.33 mmHg, and 100.91 mmHg), but it at 12 min was a little higher(l03.09 mmHg)(P=0.7566). As time went on, DBP according to stability, 3 min, 6 min, 9 min, and 12 minutes was decreased in hot bath(66.82 mmHg, 65.45 mmHg, 64.54 mmHg, 63.03 mmHg, and 59.39 mmHg)(P=0.0906). It was similar in cool bath(66.82 mmHg, 67.87 mmHg, 68.48 mmHg, 67.87 mmHg, and 68.78)(P=0.9654). As time went on, PF was significantly increased in hot bath(70.42 times, 86.96 times, 93.57 times, 99.30 times, and 101.78 times)(P=0.0001). It was a little increased in cool bath, too (70.42 times, 70.85 times, 71.63 times, 71.06 times, and 71.45 times)(P=0.9803). As time went on, RF was significantly increased in hot bath(15.75 times, 19.09 times, 22.09 times, 24.94 times, and 26.48 times)(P=0.0001). I t in cool bath of stability, 3 min, and 6 min was a little increased(15.75 times, 19.30 times, 19.39 times), but it in 9 min(18.67 times), and 12 min(18.09 times) was a little decreased(P=0.0176). As time went on, BT was significantly increased in hot bath($36.63^{\circ}C,\;37.45^{\circ}C,\;37.81^{\circ}C,\;38.12^{\circ}C,\;38.33^{\circ}C$)(P=0.0001). It was a little increased in cool bath of stability and 3 min($36.63^{\circ}C,\;37.40^{\circ}C$), but others are similar($37.33^{\circ}C,\;37.37^{\circ}C$, and $37.36^{\circ}C$)(P=0.0001). It was revealed by this study, SBP and DBP according to time in hot and cool bath were decreased. PF, RF, and BT in hot bath were higher, RF and BT in cool bath were higher too. but PF was similar.

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Carcass Characteristics, Chemical Composition and Fatty Acid Profile of Longissimus Muscle of Bulls and Steers Finished in a Pasture System Bulls and Steers Finished in Pasture Systems

  • Aricetti, Juliana Aparecida;Rotta, Polyana Pizzi;do Prado, Rodolpho Martin;Perotto, Daniel;Moletta, Jose Luiz;Matsushita, Makoto;do Prado, Ivanor Nunes
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.10
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    • pp.1441-1448
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    • 2008
  • This experiment was carried out to study the carcass characteristics, chemical composition and fatty acid profile of the Longissimus muscle (LM) of bulls (10) and steers (17) finished in a pasture system. Animals (1/2 Zebu vs. 1/2 Aberdeen Angus) were fed in a pasture system (Hermatria altissima) and with a supplement of soybean meal, cracked corn, urea, limestone and mineral salts, twice a day. Both animal groups were slaughtered at 27 months of age, with an average 508.88 kg of live weight. Final weight, hot carcass weight and texture were similar (p>0.05) between bulls and steers. Carcass dressing, fat thickness, color and marbling were higher (p<0.02) in steers. Conversely, the Longissimus area was greater (p<0.05) in bulls. Moisture levels were higher (p<0.01) in bulls. Ash, crude protein, total lipids and total cholesterol levels were higher (p<0.10) in steers. C14:0, C16:0, C16:1 n-7 and C18:1 n-9 fatty acids percentages were higher (p<0.06) in steers. C16-1 n-10, i 17:0, C19:0, C18:2 n-6, C18:3 n-3, C20:2 n-3 C20:4 n-6, C20:5 n-3, C22:1 n-7, C22:4 n-6 and C22:5 n-3 fatty acids percentages were higher (p<0.05) in bulls. The percentage of the other fatty acids was similar (p>0.05) in bulls and steers. The levels of monounsaturated fatty acids were higher (p<0.02) in steers while polyunsaturated fatty acids, n-3, n-6, PUFA/SFA and n6:n3 ratios, were higher (p<0.05) in bulls. The CLA contents (mg/g lipids) were similar (p>0.10) in bulls and steers.

Drying characteristics and physical properties of medicinal and edible mushrooms (약용버섯과 식용버섯의 건조방법에 따른 품질특성)

  • Kim, Bo-Min;Jung, Eun-sun;Aan, Yong-hyun;Hwang, In-Wook;Chung, Shin-Kyo
    • Food Science and Preservation
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    • v.23 no.5
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    • pp.689-695
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    • 2016
  • In this study, the drying characteristics of medicinal (Ganoderma lucidum, Phellinus linteus) and edible (Pleurotus eryngii, Lentinus edodes) mushrooms were investigated. Their shrinkage ratios, Hunter's color values, and rehydration ratios were evaluated. Mushrooms were cut with rectangular (1 cm) and square ($1{\times}1cm$) shapes, and dried in the room temperature and at $50^{\circ}C$ and $70^{\circ}C$ using hot-air dryer. Initial moisture contents of edible mushrooms were higher than those of medicinal mushrooms, whereas final moisture contents were vice versa. Drying rate was the highest when drying at $70^{\circ}C$ (p<0.05). Moreover the drying rate of square slices was higher than that of rectangular slices (p<0.05). The shrinkage ratios of both medicinal mushrooms and edible mushrooms were the least when drying at $50^{\circ}C$ and $70^{\circ}C$, respectively (p<0.05). The changes of color values were less in the medicinal mushrooms dried in the room temperature than in the hot-air dried medicinal mushrooms (p<0.05). However, in case of edible mushrooms, the changes of color values were less in the hot-air dried mushrooms (p<0.05). Rehydration ratios of medicinal mushrooms dried at $50^{\circ}C$ was the highest (p<0.05). In contrast, rehydration ratios of edible mushrooms was the highest when drying in the room temperature (p<0.05). Thus, the hot-air drying at $50^{\circ}C$ would be suggested as the efficient drying method for both medicinal mushrooms and edible mushrooms.

FINITE ELEMENT ANALYSIS OF STRESSES AND DEFLECTIONS INDUCED BY FIXED PARTIAL DENTURE USING ENDOSTEAL IMPLANT (골내 임프란트를 이용한 고정성 국소의치 하에서 변위 및 응력에 관한 유한요소법적 분석)

  • Choi, Su-Ho;Chung, Chae-Heon
    • The Journal of Korean Academy of Prosthodontics
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    • v.29 no.1
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    • pp.233-248
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    • 1991
  • The purpose of this study was to qunatatively analyze the stress patterns induced in the abutment, superstructure, supporting bone and to determine the deflection of abutment and superstructure by appling occlusal force to natural teeth supported fixed prostheses and implant-supported fixed prostheses. The analysis has been conducted by using the two dimensional finite element method. The implant and natural tooth-supported bridge has a first molar pontic supported by mandibular second bicuspid and implant posterior retainer, which were rigidly(Model A) or flexible(Model B). The natural teeth-supported bridge has a first molar pontic supported by mandibular second bicuspid and second molar, which were rigidly splinted together(Model C). 63.5kg(Load P1) of localized load on central fossa of first molar pontic and 24kg(Load P2) of distributed load on each occlusal surface were applied respectively. 1. The coronal portion of premolar pontic and posterior abutment in fixed partial denture deflected inferiorly in order of Model B, Model C and Model A under Load P1 and Load P2. 2. Mesial displacement of the coronal portion of premolar showed in Model A, Model B and Model C under Load P1, but mesial displacement of that in Model B and distal displacement of that in Model A and Model C showed under Load P2. 3. Mesial displacement of the coronal portion of the pontic and distal displacement of the coronal portion of posterior abutment showed in Model A, Model B and Model C under Load P1 and Load P2. Displacement in the case of Model B was greater than that of Model A and Model C. 4. In the case Model A under Load P1 and Load P2, high stress apically was concentrated in the mesiocervical portion of the posterior abutment than in the disto-cervical portion of the premolar. 5. In the case of Model B under Load P1 and Load P2 high stress was concentrated in the case of the premolar than in that of posterior abutment and high stress especially was concentrated in the connected portion of pontic and posterior abutment. 6. In the case of Model C under Load P1 and Load P2, high stress was concentrated in the distal area of the cornal portion of premolar and the mesial area of the coronal portion of posterior abutment, and stress pattern was anteroposterially symmetric around the pontic. 7. Load P1 and Load P2 compared, stress magnitude was different but stress pattern was similar in Model A, Model B and Model C. 8. Under Load P1 and P2, stress magnitude in the mesial distal portion and the portion of root apex of the posterior abutment was in order of Model B, Model A and Model C.

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The Soluble Form of the Cellular Prion Protein Enhances Phagocytic Activity and Cytokine Production by Human Monocytes Via Activation of ERK and $NF-{\kappa}B$

  • Jeon, Jae-Won;Park, Bum-Chan;Jung, Joon-Goo;Jang, Young-Soon;Shin, Eui-Cheol;Park, Young Woo
    • IMMUNE NETWORK
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    • v.13 no.4
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    • pp.148-156
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    • 2013
  • The $PrP^C$ is expressed in many types of immune cells including monocytes and macrophages, however, its function in immune regulation remains to be elucidated. In the present study, we examined a role for $PrP^C$ in regulation of monocyte function. Specifically, the effect of a soluble form of $PrP^C$ was studied in human monocytes. A recombinant fusion protein of soluble human $PrP^C$ fused with the Fc portion of human IgG1 (designated as soluble $PrP^C$-Fc) bound to the cell surface of monocytes, induced differentiation to macrophage-like cells, and enhanced adherence and phagocytic activity. In addition, soluble $PrP^C$-Fc stimulated monocytes to produce pro-inflammatory cytokines such as $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6. Both ERK and $NF-{\kappa}B$ signaling pathways were activated in soluble $PrP^C$-treated monocytes, and inhibitors of either pathway abrogated monocyte adherence and cytokine production. Taken together, we conclude that soluble $PrP^C$-Fc enhanced adherence, phagocytosis, and cytokine production of monocytes via activation of the ERK and $NF-{\kappa}B$ signaling pathways.