• Applied Biological Chemistry
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• v.44 no.4
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• pp.219-223
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• 2001

Effects of hemoglobin (Hgb) concentration and degree of hydrolysis (DH) of Hgb on the separation of heme-iron were examined to produce highly enriched heme-iron from Hgb hydrolysate. Separation efficiency of Hgb hydrolysate with different DH was studied at wide pH range (pH $1.0{\sim}11.0$). Separation efficiency expressed as heme-iron/peptide ratio increased with decreasing Hgb concentration. When 5% Hgb (pH 10.0) was hydrolyzed using commercially available Esperase for 5 h at $50^{\circ}C$, DH was 25%. The precipitation of heme-iron-enriched peptides were remarkably high at pH range $3{\sim}6$. Optimal pH range for heme-iron with high heme-iron/peptide ratio shifted to acidic pH with increasing DHs of Hgb. The enriched heme-iron fraction in the precipitates showed a single band through urea-SDS-PAGE, with a molecular mass of 1 kDa. In the dry heme-iron product produced in a pilot bioreactor, content of heme-iron and heme-iron/peptide ratio were 27.1 and 38.7%, respectively, and production yield was 9.3%.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.189-204
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• 2019

In this study, we analyzed the changes in glucosinolate content and gene expression in TO1000DH3 and Early big seedling upon methyl jasmonate (MeJA) treatment. Analysis of glucosinolate contents after MeJA treatment at $200{\mu}M$ concentration showed that the total glucosinolate content increased by 1.3-1.5 fold in TO1000DH3 and 1.3-3.8 fold in Early big compared to those before treatment. Aliphatic glucosinolates, progoitrin and gluconapin, were detected only in TO1000DH3, and the changes in the content of neoglucobrassicin were the greatest at 48 hours after MeJA treatment in TO1000DH3 and Early big. The transcriptomic analysis showed that transcripts involved in stress or defense reactions, or those related to growth were specifically expressed in TO1000DH3, while transcripts related to nucleosides or ATP biosynthesis were specifically expressed in Early big. GO analysis on transcripts with more than two-fold change in expression upon MeJA treatment, corresponding to 12,020 transcripts in TO1000DH3 and 13,510 transcripts in Early big, showed that the expression of transcripts that react to stimulus and chemical increased in TO1000DH3 and Early big, while those related to single-organism and ribosome synthesis decreased. In particular, the expression increased for all transcripts related to indole glucosinolate biosynthesis, which is associated with increase in glucobrassicin and neoglucobrassicin contents. Upon MeJA treatment, the expression of AOP3 (Bo9g006220, Bo9g006240), TGG1 (Bo14804s010) increased only in TO1000DH3, while the expression of Dof1.1 (Bo5g008360), UGT74C1 (Bo4g177540), and GSL-OH (Bo4g173560, Bo4g173550, Bo4g173530) increased specifically in Early big.

• Electronics and Telecommunications Trends
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• v.27 no.3
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• pp.32-42
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• 2012

본고에서는 디지털 홀로그래피 기술을 디지털 콘텐츠 관점에서 다룰 수 있는 연구 개발 결과를 다루었다. 디지털 홀로그래피(DH: Digital Holographic) 기술을 디지털 홀로그래픽 콘텐츠 관점으로 접근하기 위한 기반 기술 분야로서 DH 콘텐츠 저작도구 기술, 매니지먼트 기술, 공간 인식 기반 인터랙션 기술, 화질 평가 및 휴먼팩터 기술 및 응용 콘텐츠 제작 기술 등으로 분류하여 기술하였다. 또한 디지털 홀로그래픽 콘텐츠 제작을 위한 연구 개발 결과로서 3차원 영상정보 획득, 홀로그래픽 프린지 데이터 생성과 DH 콘텐츠의 광학적 수치적 복원 및 DH 콘텐츠의 운용 테스트베드 등의 구현 결과를 제시하였다. 본고에서 제시한 디지털 홀로그래픽 콘텐츠 제작을 위한 기반 기술을 향후 실용화될 디지털 홀로그램의 광학적 복원 장치와 접목한다면 새로운 형태의 홀로그래픽 실감형 콘텐츠가 활성화될 수 있을 것으로 예상된다.

• Proceedings of the IEEK Conference
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• 2000.06b
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• pp.286-289
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• 2000

PHEMT's with three different doping structures, -SH(single-heterojunction), DH (double-heterojunction), and DC(doped-channel)-,were designed, fabricated and characterized to study the effect of doping layer structures on the performance of millimeter-wave PHEMT's. 0.25${\mu}{\textrm}{m}$ DH-PHEMT with below-channel doping of 1$\times$10$^{12}$ c $m^{-2}$ was superior to SH-PHEMT by 40％ in $I_{dss}$, 20％ in f/sib T/ and showed broador gm- $I_{D}$ characteristics which is advantageous to power applications DH-PHEMT showed similar DC and small-signal performance compared with DC-PHEMT. Taking the much higher carrier mobility into considerations, DH-PHEMT is believed to be the best candidate for millimeter-wave, low-noise and/or power applications.s.s.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.1C
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• pp.117-123
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• 2008

We propose an improved Diffie-Hellman(DH) key agreement protocol over a radio link in peer-to-peer networks. The proposed protocol ensures a secure establishment of the shared key between two parties through distance bounding(DB). Proposed protocol is much improved in the sense that we now reduce the number of messages exchanged by two, the number of parameters maintained by four, and 2(7682(k/64)-64) of XOR operations, where k is the length of the random sequence used in the protocol. Also, it ensures a secure reusability of DH public parameters. Start after striking space key 2 times.

• Applied Biological Chemistry
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• v.39 no.6
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• pp.430-436
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• 1996

The induction by xylose and repression by glucose of xylose isomerase(XI) were investigated to elucidate the regulation for production of XI in Escherichia coli. Regulation for expression of xyIA gene which codes XI is under control of xylR which is a regulatory gene for xylose catabolism. When xyIR gene was resided in chromosome, the inductions of XI by the addition of 0.4% xylose were increased to 1.9 and 1.7-fold in case of locating on multicopy(pEX202/DH77) and low copy Plasmid(pEX102/DH77), respectively, as compared with that of xylA gene which was resided in chromosome(JM109). xyIR gene product derived from xyIR gene on chromosome might react to xylA gene on the plasmid as same as xylA gene on chromosome. In JM109 and xylA transformant; pEX202/DH77 and pEX102/DH77, the inductions of XI were completely repressed by the addition of 0.2% glucose and these catabolite repressions were derepressed by the addition of 1 mM cAMP In comparison with the addition of 0.4% xylose only for the induction XI was inductively produced 1.7 to 2-fold with the addition of xylose plus 1 mM cAMP in DM minimal media. pEX13/TP2010, xylA transformant of the deficient mutant($xyl^-,\;cya^-$; TP2010) of XI and cAMP production, did not induce XI by the addition of xylose only but induced in case of simultaneous addition of xylose and cAMP. These results show that cAMP and xylose are the indispensable effectors for the induction and derepression of Xl in E. coli.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.8
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• pp.1118-1123
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• 2009

Brown-rice hydrolyzates with different degrees of hydrolysis (DH) were fermented using Leuconostoc mesenteroides (Ln. mesenteroides) KC51 strain at $30^{\circ}C$ for 15 hr. Changes in pH, titratable acidity, viable cell counts and phytate degradation during fermentation were investigated. The acid production was increased with increasing DH of brown-rice hydrolyzate. At high DH (48.2%), the pH and titratable acidity reached to pH 3.41 and 0.82% after 15 hr fermentation, respectively. Regardless of DH of brown-rice, however, the viable cell population of Ln. mesenteroides KC51 was slightly increased to $4.0\sim7.2{\times}10^8$ CFU/g during the 6 hr of cultivation. The phytate content in brown-rice hydrolyzates decreased with increasing DH of brown-rice hydrolyzates. The level of phytate was reduced to around 50% of initial concentration at high DH condition. When the fermented brown-rice was kept at $4^{\circ}C$, pH, titratable acidity and number of viable cells were nearly maintained for 14 days.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.29 no.4
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• pp.202-211
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• 2017

In this study, the exergy that can be reflected in the energetic and economic values was used to assess the heat tariff of a district heating (DH) system instead of the enthalpy. It is difficult to directly apply the exergy to the current heat-charge system because of the complicated calculation; therefore, the difference between the supply and return temperatures was converted to the exergy-temperature difference for the ease of the heat-amount calculation. As a result of the exergy analysis for a DH substation, the exergy-temperature difference did not affect the surrounding temperature and pressure loss. The supply temperature and the maximum difference between the supply temperature and the return temperature exerted the main effect on the exergy-temperature difference. The new heat charge of a DH user was slightly reduced in winter compared with the previous charge, but the heat charges in the other seasons are almost the same. It is concluded from the assessment of the heat tariff for which the exergy is used that this tariff is more feasible for both DH suppliers and consumers compared with enthalpy.

• Proceedings of the KIEE Conference
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• 1988.07a
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• pp.469-471
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• 1988

The basic modeling is analyzed on the optoelectronic properties of CSP-DH laser structure using self-consistent calculation of optical field and the electron-hole distribution in the active region. Laser properties is modelled include gain profile, threshold, near field and far field pattern. This new characterization is allowed for consideration such as carrier spatial hole burning due to strong optical fields which stimulate recombination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.419-423
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• 2003

Bacterial blight caused by Xantomonas oryzae pv. oryzae is one of the most serious diseases of rice especially in southern area of Korea. Three races, $\textrm{K}_1$, $\textrm{K}_2$ and $\textrm{K}_3$, are the most dominant species. lo improve rice breeding efficiency using marker assisted selection, some RFLP markers were surveyed for polymorphism between resistant and susceptible to $\textrm{K}_1$ and $\textrm{K}_3$. And, 127 doubled-haploid (DH) lines derived from Milyang121/HRl1650-1-4-2 and 131 DH lines derived from Milyang123/HR10624-AC5 were evaluated to bacterial blight ($\textrm{K}_1$ and $\textrm{K}_3$). Milyang121 and HR10624-AC5 have Xa-1, resistant to $\textrm{K}_1$ race, and Milyang123 has Xa-3, resistant to $\textrm{K}_1$ and $\textrm{K}_3$ race. Three markers, RZ590, RZ536 and RG303, showing polymorphism between parents and resistance gene, Xa-1 and Xa-3, were analysed in the two combinations of DH lines. The segregation pattern of resistant DH population of Milyang123/HR10624-AC5 to susceptible showed 3:1 and 1:1 in $\textrm{K}_1$ and $\textrm{K}_3$ race. In three RFLP markers, RZ590 was linked to Xa-1 on chromosome 4, and RZ536 and RG303 were linked to Xa-3 on chromosome 11. The map distance between Xa-1 and RZ590 was 3.1cM on chromosome 4, and Xa-3 and RZ536/RG303 were 7.6/16.0cM on chromosome 11, respectively. The results of RFLP mapping will be useful for the selection and pyramiding of bacterial blight resistant genes.