• 농촌계획
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• 제22권3호
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• pp.119-129
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• 2016

The aim of this study is to suggest the quantitative standard of a marginal village. For the purpose, the study selected 43 villages with the population of 50 or less, the classification of a marginal village in accordance with advanced research and 47 villages with the population over 50 in Buyeo-gun region. The common reason of occurrence of a marginal village suggested by advanced research is a characteristic of a village or decline and extinction of a village rather than a simple index of population or aging rate. Therefore, the study assumed that decline of functions of the village would be caused by decline a function of communities consisting of the villagers. The study then assumed that the relatively low or 0 number or rate of participants would result in community functions. The study conducted t-test on basis of population and aging rate and an analysis to find the range with relatively large differences in the number of communities, participants and the rate of the participants, etc. The result showed that the community function began to decline when the population was less than 60~70 and the aging rate over 75%~85%. As the decline of functions of communities began in population of 70, the critical point was met when the population was 40 or less. With population of 40 or less, the young and the old group communities became extinct or showed rapid decrease in the number of participants. The study assumed that decline of functions of a village, a reason of occurrence of a marginal village would be decline of functions of communities, but there was no further analysis on decline or extinction of a village with population of 40 or less. There shall be further studies about whether a village of population of 40 or less is led to decline of a function or extinction of village communities.

• 한국응용곤충학회지
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• 제55권4호
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• pp.471-475
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• 2016

버섯파리는 국내 양송이 균상 재배지에서 발생하여 버섯 균사와 자실체를 가해하고 간접적으로는 병을 매개하여 피해는 해마다 증가하고 있다. 부여군 세도면, 보령시 성주면, 용인시 남사면, 칠곡군 약목면 등 총 4개 지역의 버섯 재배사에서 끈끈이트랩을 이용하여 채집한 버섯파리 성충을 DNA barcoding 방법으로 Cytochrome Oxidase I (COI)의 염기서열 분석을 통해 종 동정을 실시하였다. 그 결과 4개 조사지역의 양송이에서 발생하는 버섯파리는 모두 긴수염버섯파리(Lycoriella ingenua)로 동정되었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제13권1호
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• pp.77-91
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• 2008

본 연구에서는 지하수 오염 취약성 평가 방법 중 가장 활용도가 높은 DRASTIC 기법 및 지하수 수치모사 기법을 병행하여 충청남도 부여군 부여읍 일대의 지하수 오염 취약성 변화를 평가하였다. 특히, DRASTIC 기법에서 사용되는 7가지 수리인자들 중 취약성에 대한 민감도가 상대적으로 높고 비교적 장기간 자료의 획득이 용이하지 않은 '지하수위(depth to water)' 인자에 대하여 수치모사 기법을 적용하여 시-자료에 대한 동적 보간을 실시하였으며, 이를 통하여 시간에 따른 지하수위 변화가 지하수 오염 취약성에 미치는 영향을 검토하였다. 연구의 대상 지역은 부여군 부여읍 일대로 충적 대수층을 대상으로 기 실시된 지하수 수위자료를 보정대상으로 하였으며 연구지역의 지하수위 분포양상을 모사하기 위하여 유한차분모델인 MODFLOW를 이용하였다. 지하수 수치모사에는 정류 및 부정류 모사가 모두 실시되었다. 그 결과 기존 지구통계기법을 통한 지하수위 보간에서 가장 큰 가중치를 갖는 지형적 요인뿐만 아니라 유역 암상이나 지질특성 등과 같은 제반 수리지질학적 요인까지 복합적으로 반영하는 지하수위 자료를 얻을 수 있었다. 기존 연구에서 수위자료를 보완할 때 주로 이용되는 기법인 크리깅은 수치모사와는 달리 강우나 계절적 영향, 암상이나 지질등과 같은 요인들은 반영하지 못한다. 또한 수치모사를 통하여 얻어진 수위자료는 부정류 모사가 가능함으로 인하여 풍수기와 갈수기 그리고 평수기의 지하수위 변화 등도 파악할 수 있다는 장점이 있다. 모사를 통하여 얻어진 지하수위 자료와 공동크리깅 방법을 통해 얻어진 지하수위 자료를 입력자료로 하여 연구지역에 지하수 오염 취약성을 비교 평가한 결과 평수기의 경우 유사한 경향의 지하수 오염 취약성을 보였다. 또한 공동크리깅을 통해 분석이 어려운 분기별 지하수 오염 취약성 평가 결과 강우량이 많은 여름철을 포함한 분기와 강우량이 가장 적은 겨울철을 포함한 분기가 뚜렷한 취약성 차이를 보이는 것으로 분석되었다. 본 연구에서 제시하는 바와 같이 지하수 오염 취약성 평가에 있어 가장 큰 민감도를 갖는 수위자료의 수가 공간적 혹은 시간적으로 부족한 경우 수치모사 적용을 통해 자료의 한계성을 보완할 수 있으며 시간적인 보간이 이루어질 수 있어 유용할 수 있을 것이라 판단된다.

• 원예과학기술지
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• 제34권2호
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• pp.242-248
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• 2016

This research was conducted to determine the effects of four different substrates, expanded rice hulls (ERH), commercial substrates for strawberries (CSS), clay sand (CS), and loamy sand (LS), on the inhibition of anthracnose crown rot (ACR) in strawberry. Mother plants of 'Seolhyang' strawberry were transplanted into an elevated bed in March, 2013 and March, 2014 and the runners connecting mother plants and daughter plants were cut in early August of both years. After separation, growth characteristics of the daughter plants were measured and then each daughter plant was inoculated with conidial suspensions of Colletotrichum fructicola, one of several species of Colletotrichum that causes ACR in strawberries. The incidence of ACR as influenced by the different substrates was investigated in both years. The daughter plants grown on CSS had the highest values for shoot height, leaf area, and fresh weight. Those grown on ERH and LS substrates also displayed good above-ground growth characteristics except for fresh weight, but the daughter plants grown on CS had the poorest above-ground growth characteristics. The ERH and CS treatments resulted in the highest number of primary roots and the greatest root weight. The CSS-grown daughter plants had the highest ACR disease index, followed by the CS and LS treatments, but there were no significant differences among the three substrates. However, the ERH-grown daughter plants had a markedly lower ACR disease index on October 11, 2013 and October 7, 2014. The CSS-grown daughter plants had high nitrogen and potassium contents and low calcium content, whereas the ERH-grown daughter plants had low nitrogen levels and high silicon levels. The results of this study provide basic information on the ability of the different substrates tested to provide disease suppression of ACR in the propagation of strawberry transplants.

• 농업과학연구
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• 제4권2호
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• pp.178-190
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• 1977

충남(忠南) 부여군(扶餘郡) 은산면(恩山面) 라령리(羅嶺里) 소재(所在) 본대학(本大學) 연습림(演習林)은 온대(溫帶) 중부(中部)의 참나무류(類)의 극성상(極盛相) 임분(林分)이 대부분(大部分) 파양(破壤)되어 남면(南面)과 산봉(山峰)을 연결(連結)하는 능선(稜線) 부분(部分)은 소나무 단순림(單純林)의 준극성상(準極盛相)을 이루고 있다. 식생(植生)은 비교적(比較的) 다양(多樣)하며 주요(主要) 수종(樹種)은 소나무, 상수리나무, 굴참나무, 갈참나무, 졸참나무, 비목나무 굴피나무등이며 목본 53과 203종, 초본 49과 128 종이다. 이들 식물(植物)을 이용도(利用度)에 따라 분류(分類)하면 다음과 같다. 식용(食用) 식물(植物) 60과 154종, 약용(藥用) 식물(植物) 63과(科) 143종(種) 초자원(草資源) 식물(植物) 51과 140종, 관상(觀賞) 식물(植物) 32과(科) 78종(種), 용재수종(用材樹種) 12과 27종등이다.

• 한국버섯학회지
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• 제16권3호
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• pp.218-224
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• 2018

An auxin-producing bacterium, Klebsiella michiganensis Jopap-1, was isolated from a button mushroom bed in Buyeo-Gun, Chungcheongnam-Do. The strain Jopap-1 was classified as a novel strain of K. michiganensis based on a chemotaxonomic and phylogenetic analysis. The isolated K. michiganensis Jopap-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of auxin hormones by TLC and HPLC analyses. The maximum concentration of IAA ($96.05mg\;L^{-1}$) was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at $35^{\circ}C$ by HPLC quantity analysis. A negative relationship between IAA production and pH variation was estimated to show that the increase of IAA caused acidic pH in the culture. The effect of the supplement on L-tryptophan (precursor of IAA) production was observed to be highest at 0.1% concentration, but was significantly lowered above a concentration of 0.2%. To investigate the growth-promoting effects on the crops, the culture broth of E. michiganensis Jopap-1 was infected to water cultures and seed pots of mung bean and lettuce. Consequently, the adventitious root induction and root growth of mung bean and lettuce were observed to be 2.1 and 1.8 times higher than those of the control.

• 한국버섯학회지
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• 제11권4호
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• pp.187-193
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• 2013

A galactose fermentation bacterium producing lactose from red seaweed, which was known well to compromise the galactose as main reducing sugar, was isolated from button mushroom bed in Buyeo-Gun, Chungchugnamdo province. The lactic acid bacteria MONGB-2 was identified as Lactobacillus paracasei subsp. tolerans by analysis of 16S rRNA gene sequence. When the production of lactic acid and acetic acid by L. paracasei MONGB-2 was investigated by HPLC analysis with various carbohydrates, the strain MONGB-2 efficiently convert the glucose and galactose to lactic acid with the yield of 18.86 g/L and 18.23 g/L, respectively and the ratio of lactic acid to total organic acids was 1.0 and 0.91 g/g for both substrates. However, in the case of acetic acid fermentation, other carbohydrates besides galactose and red seaweed hydrolysate could not be totally utilized as carbon sources for acetic acid production by the strain. The lactic acid production from glucose and galactose in the fermentation time courses was gradually enhanced upto 60 h fermentation and the maximal concentration reached to be 16-18 g/L from both substrates after 48 h of fermentation. The initial concentration of glucose and galactose were completely consumed within 36 h of fermentation, of which the growth of cell also was maximum level. In addition, the bioconversion of lactic acid from the red seaweed hydrolysate by L. paracasei MONGB-2 appeared to be about 20% levels of the initial substrates concentration and this results were entirely lower than those of galactose and glucose showed about 60% of conversion. The apparent results showed that L. paracasei MONGB-2 could produce the lactic acid with glucose as well as galactose by the homofermentation through EMP pathway.

• 농업과학연구
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• 제39권2호
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• pp.255-261
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• 2012

A bacterium AB-55, isolated from waste mushroom bed of Agaricus bisporus in Sukseong-myeon, Buyeo-gun, Chungcheongnam-do, Korea, was screened onto xylan agar congo-red plate by the xylanolysis method and was used to produce an xylanase in shaker buffle flask cultures containing oat spelt xylans. The phylogenetic analysis using 16S rRNA gene sequence data showed that the strain AB-55 had the highest homology (99.0%) with Bacillus subtilis and it was named as Bacillus subtilis AB-55. A xylanase was purified by ammonium sulfate precipitation (50~80%), gel filtration on sephacryl S-300, and ion exchange chromatography on DEAE sepharose FF. The molecular weight of the xylanase was estimated as 44 kDa by SDS-PAGE. Optimal pH and temperature for the xylanase activity was pH 7 and $50^{\circ}C$, respectively. N-terminal amino acid sequence of the enzyme was identified as Ser-Ala-Val-Lys-His-Gly-Ala-Ile-Val-Phe. The substrate specificity of the enzyme exhibited that it hydrolyzed efficiently oat spelt xylan as well as beechwood xylan, but showed no activity against Avicel and carboxymethyl clellulose (CMC). The enzyme activity was enhanced by $Fe^{2+}$ and $Mn^{2+}$ whereas was entirely inhibited by $Hg^+$.

• 한국토양비료학회지
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• 제44권5호
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• pp.866-871
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• 2011

An auxin-producing bacteria (3YN11-02) was isolated from waste mushroom bed from Agaricus bisporus of Chungnam Buyeo-Gun area. The strain 3YN11-02 was identified as a novel species belongs to Rahnella aquatica by a chemotaxanomic and phylogenetic nalysis. The isolate was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC and HPLC analysis. When the concentration of IAA was assessed by performing HPLC quantity analysis, the maximal $290mg\;L^{-1}$ of IAA detected in ether fraction extracted from the culture filtrate which was cultured in R2A broth containing 0.1% tryptophan for 24 h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. To investigate the growth promoting effect of crop, when the culture broth of R. aquatica 3YN11-02 was infected onto water culture and seed pot of mung bean, the adventitious root induction and root growth of mung bean were 2.0 times higher than control.

• 한국의사학회지
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• 제31권2호
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• pp.53-62
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• 2018

It is known that the development of medical science is influenced by various social environmental factors. Historically, Chinese Medicine developed the most during the Song Yuan dynasty, and the reason for this was known to be due to socio-political factors. According to recent studies, however, this period also had severe changes in climate and environment. Therefore, this study was conducted under the premise that this change in climate and environment influenced medical development. When looking at the coldness of the 11th~12th century and data indicating warming before and after this period, the Song Yuan dynasty went through drastic periods of climate change. Therefore, diseases related to heat such as bubonic plague, measles, and malaria were common. Furthermore, due to occasional wars during the Song Yuan dynasty, social unrest was aggravated and infectious diseases spread due to land development and environmental pollution. As the health of people were threatened due to these factors, the printing and distribution of medical text were encouraged, and during this process, the great 4 doctors of Jin-Yuan appeared. The reason why they studied cures for infectious diseases due to heat was related to climatic environment change. The development in medical science is closely related to socio-political factors, however the change in climatic environments are inevitably related to disease emergence as well. Therefore, it should always be taken into consideration as an important factor that promotes development in medical science.