한국응용곤충학회지 (Korean journal of applied entomology)

  • 제55권4호
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  • Pages.471-475
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  • 2016
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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국내 양송이버섯 재배 중 발생하는 버섯파리류 분자생물학적 종 동정

Molecular Identification of the Dominant Species of Dark-winged Fungus Gnat (Diptera: Sciaridae) from Button Mushroom (Agaricus bisporus) in Korea

  • 윤정범 (국립원예특작과학원 원예특작환경과) ;
  • 김형환 (국립원예특작과학원 원예특작환경과) ;
  • 정충렬 (국립산림과학원 산림약용자원연구소) ;
  • 강민구 (경상북도농업기술원 농업환경과) ;
  • 권선정 (국립원예특작과학원 원예특작환경과) ;
  • 김동환 (국립원예특작과학원 원예특작환경과) ;
  • 양창열 (국립원예특작과학원 원예특작환경과) ;
  • 서미혜 (국립원예특작과학원 원예특작환경과)
  • Yoon, Jung-Beom (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Hyeong-Hwan (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jung, Chung-Ryul (Forest medical resources research center, National Institute of Forest Science) ;
  • Kang, Min-Gu (Gyeongbuk-do Agricultural Research and extension Services) ;
  • Kwon, Sun-Jung (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Dong-Hwan (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yang, Chang-Yeol (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Seo, Mi-Hye (Horticultural and herbal crop environment division, National Institute of Horticultural and Herbal Science, RDA)
  • 투고 : 2016.08.29
  • 심사 : 2016.11.08
  • 발행 : 2016.12.01
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초록

버섯파리는 국내 양송이 균상 재배지에서 발생하여 버섯 균사와 자실체를 가해하고 간접적으로는 병을 매개하여 피해는 해마다 증가하고 있다. 부여군 세도면, 보령시 성주면, 용인시 남사면, 칠곡군 약목면 등 총 4개 지역의 버섯 재배사에서 끈끈이트랩을 이용하여 채집한 버섯파리 성충을 DNA barcoding 방법으로 Cytochrome Oxidase I (COI)의 염기서열 분석을 통해 종 동정을 실시하였다. 그 결과 4개 조사지역의 양송이에서 발생하는 버섯파리는 모두 긴수염버섯파리(Lycoriella ingenua)로 동정되었다.

The dark-winged fungus gnats are one of the most serious fly pests attacking the mushroom cultivation in Korea. They cause severe damage to the artificial sawdust beds used to cultivate mushroom, and reduce the production of button mushroom, Agaricus bisporus, in greenhouses. In this study, we collected nine species of the mushroom flies in order to identify the dominant species of the dark-winged fungus gnat attacking the A. bisporus plantation using the yellow sticky trap in Buyeo-gun, Boryeong-gun, Yongin-si and Chilgok-gun from April to June 2015. The collected samples were used to determine the DNA sequence of the cytochrome c oxidase subunit I (COI) of the nine different species by DNA barcoding. The sequencing results showed that Lycoriella ingenua was the dominant dark-winged fungus gnat species destroying A. bisporus cultivated on the artificial sawdust beds in Korea.

키워드

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피인용 문헌

  1. Control of Dark-winged Fungus Gnats (Lycoriella ingenua) using Mist Fogger with Natural Pyrethrum (Tanacetum cinerariifolium) vol.21, pp.3, 2017, https://doi.org/10.7585/kjps.2017.21.3.279