• The Korean Journal of Food And Nutrition
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• v.13 no.5
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• pp.460-464
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• 2000

We have evaluated cytotoxic effects of four crude extracts of methylene chloride, ethyl acetate, butanol, water layer isolated Rheum undulatum in A498 cell line, human kidney epithelial cells. The cytotoxic evalutation was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) , neutral red(NR) and sulforhodamine protein B(SRB). These results obtained are as follows : MTT, NR and SRB quantities were significantly decreased in cultured A498 cells treated four crude extracts by increased concentrations. The cell cytotoxic effect of crude extracts of butanol layer was more stronger than others layer. The values of MTT$\sub$50/, NR$\sub$50/, SRB$\sub$50/ of crude extract of butanol layer and were measured both 0.63 mg/ml, 0.65 mg/ml, and 0.68 mg/ml, respectively and the values of water layer were 0.84 mg/ml, 0.82 mg/ml. and 0.80 mg/ml. respectively in cultured A498 cell line.

• Journal of Conservation Science
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• v.16 s.16
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• pp.110-118
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• 2004

In order to evaluate two-step treatment of PEG-freeze drying for highly-degraded waterlogged ash woods (Fraxinus PP.; ca. 5,700 BP), which were excavated from peat lands in western Korea, dimension stability was examined during 45 months after complete treatment. The samples pre-treated with PEG in water solution showed better dimensional stabilities than the ones with PEG in t-butanol(TBA) solution. It suggests that TBA reduced the flexibility of wood cells and overflying by TBA induced micro-checks during freeze drying. Micro-checks results in fragile wood structures and consequently, large shrinkage by moisture absorbances of high PEG contents during exposure in humid condition. The results suggest that PEG in water-solution treatment is better than PEG in t-butanol as pretreament for freeze drying of highly-degraded waterlogged ash woods.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.180-186
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• 1991

A water soluble antifungal antibiotic, Solumycin, was separated from the culture broth of Streptomyces sp. LAM-593, isolated from soil, by butanol extraction, alumina-, 1st and 2nd Sephadex LH-20 column chromatography. The substance was pale yellow crystal which gave a single spot at Rf value 0.24 with ethanol-ammonia water-water (8:1:1), 0.46 with butanol-ethanol-water (5:1:4), 0.84 with 50% methanol on silica gel TLC. It was dissolved well in water, methanol and acidic aq. butanol but not in ethanol, acetone, ethyl acetate, chloroform. acetic acid etc., and gave positive Fehling and Molish reaction. The UV spectrum in methanol showed absorption at 342, 361, 380, and 404 nm. The antibiotic was active against fungi such as Candide, Cryptococcus, Saccharomyces, Trichophyton and Trichosporon, but not to bacteria such as Bacillus, Escherichia and Staphylococcus.

• Membrane Journal
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• v.19 no.2
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• pp.157-164
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• 2009

Bio-butanol recovery by pervaporation was performed with dense and composite polydimethylsiloxane (PDMS) membranes. The pervaporative behavior of the membranes was investigated as a function of operation temperature $(20{\sim}40^{\circ}C)$ and membrane thickness $(100{\sim}1{\mu}m)$ using a series of aqueous BtOH model solutions $(1{\sim}5wt%)$. With the increment of the BtOH concentration in feed, the Butanol concentration in permeate, pervaporation selectivity of Butanol over water and Butanol permeation flux increased. As the operating temperature of feed solutions increased, the BtOH concentration in permeate, pervaporation selectivity and permeation flux increased markedly. As the thickness of the PDMS membrane decreased, permeation flux increased but pervaporation selectivity decreased. These results were explained in terms of high solubility and low diffusion resistance of BtOH over water toward hydrophobic and rubbery PDMS membranes.

• Korean Journal of Food Science and Technology
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• v.29 no.3
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• pp.432-436
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• 1997

This study was conducted to investigate the functional characteristics of diethylether and butanol extracts from Ganoderma lucidum, Agaricus bisporus and Lentinus edodes. Electron donating abilities of diethylether and butanol extracts from Ganoderma luculum were 95.07% and 97.75%, respectively. Electron donating abilities of butanol extracts from Agaricus bisporus was 94.33%, and that of Lentinus edodes was 96.09%. Antioxidative activities of diethylether and butanol extracts from Canoderma lucidum were higher than those of BHA. All extracts of Agaricus bisporus and Lentinus edodes showed lower antioxidative activity than that of BHA. Nitrite-scavenging abilities of diethylether and butanol extracts from Ganoderma lucidum were 68.34% and 44.44%, respectively. Nitrite-scavenging abilities of butanol extracts from Agaricus bisporus were 43.39%, and those of Lentinus edodes were 68.23%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.6
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• pp.948-951
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• 2003

The composition and antioxidative effects of phenolic compounds in black rice were studied. The contents of free and bound phenolic compounds extracted from black rice were 845.4 and 401.6 mg respectively per 100g sample weight. Free phenolic compounds had higher antioxidation ability than those of bound phenolic compounds. Solvent fractionation of free phenolic compounds revealed that butanol fraction had the highest phenolic compounds contents and antioxidative activity among other solvent fractions. Although butanol fraction showed lower lipid peroxidation inhibition (LPI) ability than that of $\alpha$-tocopherol and BHT, free radical scavenging ability was much higher than that of $\alpha$-tocopherol and BHT, as evidenced by electron donating ability (EDA) and benzoic acid hydroxylation inhibition (BAHI) assays.

• Applied Chemistry for Engineering
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• v.22 no.5
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• pp.447-452
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• 2011

Depletion of petroleum increased the need of alternative energy and chemical resources. Biomass, a renewable resource, can be transformed to bioenergy and biomaterials, and the materials from biomass will ultimately substitute petroleum based energy and chemical compounds. In this perspective, production of C4-C6 compounds for bioenergy and biomaterials are described for understating of current research progress. n-Butanol and n-butyric acid, the major C4 compounds, are produced by Clostridium tyrobutyricum, Clostridium beijerinckii, and Clostridium acetobutylicum. n-Hexanoic acid, a typical C6 compound, is produced by Clostridium kluyveri and Megasphaera elsdenii. Reported maximum amount of n-butanol, n-butyric acid and n-hexanoic acid was 21, 55, and 19 g/L, respectively, and extraction of these C4-C6 compounds are induced increase production by those anaerobic bacteria. In addition, a new bacterium Clostridium sp. BS-1 produced 5 g/L of n-hexanoic acid using galactitol.

• Clean Technology
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• v.26 no.3
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• pp.161-167
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• 2020

For the design of the prevention and mitigation measures in process industries involving flammable substances, reliable safety data are required. An important property used to estimate the risk of fire and explosion for a flammable liquid is the flash point. Flammability is an important factor to consider when developing safe methods for storing and handling solids and liquids. In this study, the flash point data were measured for the binary systems {2-butanol + 2,2,4-trimethylpentane}, {2-butanol + methylcyclohexane} and {2-butanol + toluene} at 101.3 kPa. Experiments were performed according to the standard test method (ASTM D 3278) using a Stanhope-Seta closed cup flash point tester. A minimum flash point behavior was observed in the binary systems as in the many cases for the hydrocarbon and alcohol mixture that were observed. The measured flash points were compared with the predicted values calculated via the following activity coefficient (GE) models: Wilson, Non-Random Two-Liquid (NRTL), and UNIversal QUAsiChemical (UNIQUAC) models. The predicted data were only adequate for the data determined by the closed-cup test method and may not be appropriate for the data obtained from the open-cup test method because of its deviation from the vapor liquid equilibrium. The predicted results of this work can be used to design safe petrochemical processes, such as the identification of safe storage conditions for non-ideal solutions containing flammable components.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.6
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• pp.677-682
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• 2006

This study was conducted to investigate the effect of garlic extracts on electron donating ability nitrite-scavenging activity and N-nitrosodimethylamine (NDMA) formation. Content of total phenolic compounds was the highest in ethyl acetate extract. Electron donating ability was significantly increased with increasing sample concentration, as $88.0{\pm}1.53%$ from ethyl acetate extract and $77.5{\pm}0.8%$ from butanol extract in concentration of 10 mg/mL. Butanol extract from garlic with highest nitrite scavenging activity and inhibition effect of NDMA formation exhibited $96.9{\pm}0.75%$ and $68.6{\pm}1.15%$ in concentration of 10 mg/mL, respectively. The butanol extract was fractionated into 5 fractions using prep-LC. Nitrite-scavenging activity and inhibition effect of NDMA formation were excellent in fraction III, which further fractioned into 3 subfractions (III-a, III-b and III-c) using prep-LC. Of the 3 subfractions, its activity was the highest in the subfraction III-c. Therefore butanol extract of garlic was suggested to be effective inhibitor of NDMA formation.

• Journal of Life Science
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• v.24 no.4
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• pp.386-392
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• 2014

Haw (Crataegus pinnatifida $B_{UNGE}$) is a medicinal plant commonly used in Korea due to its beneficial health effects. This study was performed to investigate the antioxidative activity of bioactive components from haw seed. Freeze-dried seed was processed with various solvents such as 70% methanol, 70% ethanol, a mixture of chloroform and methanol (2:1, v / v), n-butanol and ethyl acetate (EA). DPPH, reducing power, ferric reducing antioxidant power (FRAP), and ABTS radical scavenging activity decreased in this order: 70% methanol, 70% ethanol, EA, CM, n-butanol extracts. NO radical scavenging activity was high in the n-butanol extract (36.73% in 0.8 mg/ml concentration) and low in the 70% ethanol extract (24.96% in 0.8 mg/ml concentration). $OH^-$ radical scavenging activity of n-butanol extract (40.42% in 0.8 mg/ml concentration) was higher than the other extracts, but EA extract was the lowest (13.39%) at the same concentration. The results show that each extract contained various antioxidative substances and it seemed that different influence at each antioxidative activity.