Effect of Garlic (Allium sativum L.) Extracts on Formation of N-Nitrosodimethylamine

This study was conducted to investigate the effect of garlic extracts on electron donating ability nitrite-scavenging activity and N-nitrosodimethylamine (NDMA) formation. Content of total phenolic compounds was the highest in ethyl acetate extract. Electron donating ability was significantly increased with increasing sample concentration, as $88.0{\pm}1.53%$ from ethyl acetate extract and $77.5{\pm}0.8%$ from butanol extract in concentration of 10 mg/mL. Butanol extract from garlic with highest nitrite scavenging activity and inhibition effect of NDMA formation exhibited $96.9{\pm}0.75%$ and $68.6{\pm}1.15%$ in concentration of 10 mg/mL, respectively. The butanol extract was fractionated into 5 fractions using prep-LC. Nitrite-scavenging activity and inhibition effect of NDMA formation were excellent in fraction III, which further fractioned into 3 subfractions (III-a, III-b and III-c) using prep-LC. Of the 3 subfractions, its activity was the highest in the subfraction III-c. Therefore butanol extract of garlic was suggested to be effective inhibitor of NDMA formation.

마늘 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향

본 연구는 마늘 추출물 중의 총 페놀함량, 전자공여능, 아질산염 소거능을 측정하고 NDMA생성억제에 미치는 영향을 검색하였다 총 페놀함량은 ethyl acetate>hexane>butanol>chloroform>water 순으로 많았으며, 전자공여능은 추출물의 농도증가에 따라 유의적으로 증가하였으며 ethyl acetate와 butanol 추출물을 10 mg/mL 첨가 시 각각 $88.0{\pm}1.53%,\;77.5{\pm}0.8%$의 활성을 보였다. 아질산염 소거능 및 NDMA 생성 억제효과는 10 mg/mL의 butanol 추출물 첨가 시 각각 $96.9{\pm}0.75%$ 및 $68.6{\pm}1.15%$로 가장 높게 나타났다. Butanol 추출물을 prep-LC로 5개의 획분으로 분획하여 아질산염 소거능 및 NDMA 생성 억제효과를 측정한 결과, fraction III에서 효과가 가장 뛰어났으며, 이를 prep-LC로 재분획하여 얻은 3개의 subfractions(III-a, III-b 및 III-c)에서는 subfraction III-c의 효과가 가장 우수하였다. 따라서 마늘 부탄올 추출물은 NDMA 생성 억제에 효과적인 저해제인 것으로 판단된다.