• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1005-1010
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• 2008

The genus Burkholderia consists of extremely versatile bacteria that occupy diverse niches and are commonly encountered in the rhizosphere of crop plants. In this study, we characterized three plant growth promoting strains assigned as Burkholderia sp. using biochemical and molecular characterization. The Burkholderia spp. strains CBMB40, CBPB-HIM, and CBPB-HOD were characterized using biochemical tests, BIOLOG carbon substrate utilization, fatty acid methyl ester analysis, analysis of recA gene sequences, and DNA-DNA hybridization. The results from these studies indicated that the strains CBMB40, CBPB-HIM, and CBPB-HOD can be assigned under Burkholderia vietnamiensis, Burkholderia ubonensis, and Burkholderia pyrrocinia, respectively.

• 한국토양비료학회지
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• 제45권4호
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• pp.602-609
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• 2012

Burkholderia anthina R-4183, Burkholderia diffusa R-15930 and Burkholderia stabilis LMG 14294 isolated from green house soils (Gongju-Gun area, South Korea) were characterized and their phosphate solubilizing ability was assessed. Under in vitro culture conditions, all three species were proved to be effective in solubilizing phosphates in varying degrees. Strain Burkholderia anthina exhibited the highest phosphate solubilization in NBRIP medium ($665{\mu}g\;ml^{-1}$) followed by Burkholderia diffusa ($630{\mu}g\;ml^{-1}$) and Burkholderia stabilis ($578{\mu}g\;ml^{-1}$). However, solubilization of $FePO_4$ and $AlPO_4$ was found to be poor in all the strains. Acidification by means of gluconic and oxalic acids accumulation in the culture medium could be the possible mechanism responsible for phosphate solubilization. Glucose at the rate of 3% was found be the best carbon source for Burkholderia anthina while other two Burkholderia species showed maximum phosphate solubilization at 2% of glucose. In the case of nitrogen sources, ammonium and nitrate were equally effective in solubilizing phosphates by Burkholderia species. Despite a slight decrease in phosphate solubilization observed at increasing temperature, all three Burkholderia species could withstand a temperature of $30-35^{\circ}C$, pH at the range of 7-9 and the presence of NaCl (up to 2.5%) without much compromising the phosphate solubilization. As shown with potted mung bean seedlings, all the three isolates could enhance soil fertility and plant growth indicating their great potential to be used as bio-inoculants.

• 약학회지
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• 제50권4호
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• pp.228-233
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• 2006

Matrix metalloproteinase-2 is known to be involved in pathological processes such as tumor invasion or rheumatoid arthritis. A soil microorganism producing siderophore under low iron stress $(up\;to\;5\;{\mu}m\;of\;iron)$ was identified as Burkholderia sp. Hydroxamate type siderophore produced by Burkholderia sp. CAS-5 was partially purified. MMP inhibitory activity of siderophore was confirmed by gelatin zymography. The $Zn^{2+}-chelating$ activity of siderophore correlated with the inhibition of MMP-2 activity.

• 환경생물
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• 제28권2호
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• pp.95-100
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• 2010

우리나라 토양으로부터 분리된 (주와 김 2009) poly (butylene succinate-co-butylene adipate; PBSA) 분해균 Burkholderia cepacia PBSA-7, Bacillus licheniformis PBSA-8 및 Burkholderia sp. PBSA-9에서 PBSA 분해효소를 암호화하는 유전자를 분석하였다. PCR을 수행하여 B.cepacia PBSA-7과 Burkholderia sp. PBSA-9는 약 1.5 Kb, B. licheniformis PBSA-8은 약 600 bp의 lipase 유전자(lip A) 절편을 가지는 것을 확인하였다. 세 균주 모두에서 유전자의 염기서열 내 lipase box인 Gly-X1-Ser-X2-Gly와 Ala-X1-Ser-X2-Gly가 존재하였다. B. cepacia PBSA-7의 PBSA 분해효소 유전자는 family I-1 lipases와 36~40%, family I-2 lipases와는 82~92%의 높은 유전적 상동성을 보였다. 또한 B. licheniformis PBSA-8의 PBSA 분해효소 유전자는 subfamily 1-4 lipases와 64~65%의 유전적 상동성을 나타내었으나, subfamily 1-5에 속하는 lipase들과는 거의 유전적 상동성이 없는 것으로 나타났다. Burkholderia sp. PBSA-9의 PBSA 분해효소 유전자도 family I-1 lipases와 35~37%, family I-2 lipases와는 83~94%로 높은 유전적 상동성을 보여 B. cepacia PBSA-7의 PBSA 분해효소 유전자와 유사한 결과를 보였다.

• Journal of Microbiology and Biotechnology
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• 제10권5호
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• pp.643-650
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• 2000

A new aniline-utilizing microorganism, strain HY1 obtained from an orchard soil, was characterized by using the BIOLOG system, an analysis of the total cellular fatty acids, and a 16S rDNA sequence. Strain HY1 was identified as a Burkholderia species, and was designated Burkholderia sp. HY1. GC and HPLC analyses revealed that Burkholderia sp. HY1 was able to degrade aniline to produce catechol, which was subsequently converted to cis,cis-muconic acid through an ortho-ring fission pathway under aerobic conditions. Strain HY1 exhibited a drastic reduction in the rate of aniline degradation when glucose was added to the aniline media. However, the addition of peptone or nitrate to the aniline media dramatically accelerated the rate of aniline degradation. A fatty acid analysis showed that strain HY1 was able to produce lipids 16:0 2OH, and 11 methyl 18:1 ${\omega}7c$ approximately 3.7-, 2.2-, and 6-fold more, respectively, when grown on aniline media than when grown on TSA. An analysison the alignment of a 1,435 bp fragment. A phylogenetic analysis of the 16S rDNA sequence based on a 1,420 bp multi-alignment sowed of the 16s rDNA sequence revealed that strain HY1 was very closely related to Burkholderia graminis with 95% similarity based that strain HY1 was placed among three major clonal types of $\beta$-Proteobacteria, including Burkholderia graminis, Burkholderia phenazinium, and Burkholderia glathei. The sequence GAT(C or G)${\b{G}}$, which is highly conserved in several locations in the 16S rDNA gene among the major clonal type strains of $\beta$-Proteobacteria, was frequently replaced with GAT(C or G)${\b{A}}$ in the 16S rDNA sequence from strain HY1.

• KSBB Journal
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• 제15권6호
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• pp.589-593
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• 2000

Several bacterial strains growing on benzene minimal medium were isolated from soil by enrichment culture, Burkholderia sp. SKK381 was identified and selected. In order to determine the ability of Burkholderia sp. SKK381 to degrade benzene. Changes in substrate concentration, cell growth, and pH were monitored from start-up in bath culture. At 30$^{\circ}C$, 1000 ppm of benzene was degraded 100% within 28hours. Cell growth conditions were best at an initial pH of 7.0 and a benzene concentration of 1000 ppm at 30$^{\circ}C$.

• 한국축산식품학회지
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• 제30권6호
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• pp.946-950
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• 2010

This study evaluated the antimicrobial effects of meat processing-related organic acids on Burkholderia thailandensis (Burkholderia pseudomallei surrogate) with different water activities. B. thailandensis KACC12027 (4 log CFU/mL) was inoculated in microwell plates containing tryptic soy broth pH-adjusted to 4, 5, 6, and 7 with ascorbic acid, citric acid, and lactic acid and with water activities adjusted to 0.94, 0.96, 0.98, and 1.0 with NaCl, followed by incubation at $35^{\circ}C$ for 30 h. The optical density (OD) of the samples was measured at 0, 3, 6, 12, 24, and 30 h at 595 nm to estimate the growth of B. thailandensis. Growth of B. thailandensis was observed only at water activity of 1.0. In general, more bacterial growth (p<0.05) was observed at pH 6 than at pH 7, and the antimicrobial effects of the organic acids on B. thailandensis were in the following order: Ascorbic acid > lactic acid > citric acid after incubation at $35^{\circ}C$ for 30 h. These results indicate that organic acids in meat processing-related formulations should be useful in decreasing the risk related to an emerging high risk agent (B. pseudomallei).

• 한국토양비료학회지
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• 제49권3호
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• pp.251-258
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• 2016

A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

• Molecules and Cells
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• 제27권2호
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• pp.237-241
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• 2009

Pathogens Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm) contain a large number (> 12,000) of Simple Sequence Repeats (SSRs). To study the extent to which these features have contributed to the diversification of genes, we have conducted comparative studies with nineteen genomes of these bacteria. We found 210 genes with characteristic types of SSR variations. SSRs with nonamer repeat units were the most abundant, followed by hexamers and trimers. Amino acids with smaller and nonpolar R-groups are preferred to be encoded by the variant SSRs, perhaps due to their minimal impacts to protein functionality. A majority of these genes appears to code for surface or secreted proteins that may directly interact with the host factors during pathogenesis or other environmental factors. There also are others that encode diverse functions in the cytoplasm, and this protein variability may reflect an extensive involvement of phase variation in survival and adaptation of these pathogens.

• 한국토양비료학회지
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• 제36권3호
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• pp.127-133
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• 2003

토양미생물의 생태적 특성을 평가하기 위해 화강암, 석회암 및 현무암을 모재로 한 12개의 토양통의 미생물적 특성을 조사하였다. 토양미생물상은 토양화학성과 유의한 상관관계를 보였는데, 특히 토양 pH는 형광성 슈도모나스와, 토양 EC는 방선균, 사상균, 중온성 Bacillus 및 호알카리성균과 정의 관계를 보였다. 포자형성균인 Bacillus는 화강암과 현무암에 비해 석회암토양에서 높은 균수를 보였으나, 그람음성균은 모암간에 비슷한 수준을 유지하였다. 화강암유래 토양에서는 Burkholderia, Pseudomonas, Ralstonia가, 석회암토양에서는 Pseudomonas, Burkholderia, Phyllobacterium가, 현무암토양에서는 Burkholderia속이 우점적으로 분포하고 있었다.