Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Characterization of Burkholderia Strains Isolated from Rice Cultivars (Oryza sativa L.) for Species Identification and Phylogenetic Grouping

  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Poonguzhali, Selvaraj (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kwon, Soon-Wo (Korean Agricultural Cultural Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Song, Myung-Hee (Korean Agricultural Cultural Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Published : 2008.06.30
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Abstract

The genus Burkholderia consists of extremely versatile bacteria that occupy diverse niches and are commonly encountered in the rhizosphere of crop plants. In this study, we characterized three plant growth promoting strains assigned as Burkholderia sp. using biochemical and molecular characterization. The Burkholderia spp. strains CBMB40, CBPB-HIM, and CBPB-HOD were characterized using biochemical tests, BIOLOG carbon substrate utilization, fatty acid methyl ester analysis, analysis of recA gene sequences, and DNA-DNA hybridization. The results from these studies indicated that the strains CBMB40, CBPB-HIM, and CBPB-HOD can be assigned under Burkholderia vietnamiensis, Burkholderia ubonensis, and Burkholderia pyrrocinia, respectively.

Keywords

References

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