• Korean Journal of Veterinary Service
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• v.35 no.4
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• pp.255-261
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• 2012

Foot and mouth disease (FMD) is highly infectious disease of cloven-hoofed animals, particularly cattle, sheep, pigs and goats. Last outbreak reported in November, 2010 induced the enormous social and economical impacts. Culling of infected animals, movement control, and vaccination are the major control measures of FMD. The aim of this study was to detection foot-and-mouth disease virus (FMDV) in the soil and leachate from modeling burial for pig carcass as measured by real-time reverse transcriptase polymerase chain reaction (RT-PCR). FMDV and Coxsakievirus B1 (CVB1) were detected in soil by week 16 and Coxsakievirus B1 (CVB1) by weeks 12, respectively. FMDV and CVB1 also detected by weeks 8 in the leachate. Results from this study provides an evidence that FMDV could be inactivated for safe of pig carcasses infected with FMDV within 4 month in the carcass burial site.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.384-389
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• 2010

Outbreak of contagious diseases to livestock animals is becoming prevalent worldwide and consequently, tremendous numbers of the infected or culled stocks are buried on the ground as the most common disposal method. The buried animals can generate a wide range of detrimental components such as leachate, nutrient salts, and pathogenic bacteria, consequently contaminating the surround environment. This implies that regular investigations are required to monitor any possible detrimental environmental aspect occurred around burial sites. Therefore, the current study was conducted to investigate whether the soil and groundwater nearby the burial sites had been contaminated by the substances originated from the burial sites, which can be applied for the establishment of the ideal burial site construction design and post management scheme. For this, two different burial sites located in Cheonan and Pyeongtaek were selected. Cheonan and Pyeongtaek sites were constructed in 2004 and 2008, respectively and both contained dead poultry infected by avian influenza (AI). Soil and groundwater samples were collected around the sites followed by determination of the nutrient concentrations and bacteria (Salmonella, Camphylobacter, and Bacillus) existence in both soil and groundwater. Some of the soil samples showed higher EC, $NH_4$-N, $NO_3$-N concentration compared to those of the background (control) soils. Also the concentration of $NH_4$-N in some of the groundwater samples appeared to exceed the USEPA guideline value for drinking water (10 mg $L^{-1}$). These results indicated that the soil and groundwater were influenced by the burial site originated nutrients. In the soil, Bacillus was isolated in most soil samples while there were no detections of Salmonella and Camplylobacter. Due to the Bacillus existing mainly as a spore in the soils, it was considered that the frequent detection of Bacillus in the soil samples was attributed to the nutrients originated from the burial sites.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.447-460
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• 2023

Fire blight, a plant disease caused by Erwinia amylovora, continues to cause considerable damage to fruit-bearing trees belonging to the Rosaceae family, such as apples and pears. In Korea, to hinder its rapid spread diseased apple and pear trees have been buried since its first occurrence. To determine the safety of this control method, the buried plant materials and adjacent soil have been investigated. In the process of investigating the microbiota of the buried soil, Bisifusarium allantoides, Botryotrichum domesticum, Microascus verrucosus, Paraphoma pye, Phaeosphaeria culmorum, Ramophialophora globispora, Sordaria tomentoalba, and Striaticonidium brachysporum were identified as unrecorded species in Korea. To report the above eight species as Korean unrecorded species, we report their morphological and molecular features in this study.

• Korean Journal of Veterinary Service
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• v.43 no.2
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• pp.107-112
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• 2020

Foot-and-mouth disease (FMD) and avian influenza (AI) are highly pathogenic viral disease which affects the livestock industry worldwide. Outbreak of these viruses causes great impact in the livestock industry; thus, disease infected animals were immediately disposed. Burial is the commonly used disposal method for deceased animals. However, there is potential for secondary environmental contamination, as well as the risk that infectious agents persisting in the environment due to the limited environmental controls in livestock burial sites during the decomposition of the carcasses. Therefore, this study aimed to investigate the detection of FMD and AI viruses from animal carcass disposal sites using real-time reverse transcription PCR. Soil samples of more than three years post-burial from livestock carcass disposal sites were collected and processed RNA isolation using a commercial extraction kit. The isolated RNA of the samples was used for the detection of FMDV and AIV using qRT-PCR. Based on the qPCR assay result, no viral particle was detected in the soil samples collected from the animal disposal sites. This indicates that 3 years of burial and their carcass disposal method is efficient for the control or at least reduction of spread infections in the surrounding environment.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.269-272
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• 2019

BACKGROUND: Since 2015, fire blight disease caused by Erwinia amylovora has been devastating apple and pear orchards every year. To quickly block the disease spreading, infected apple and pear trees have been buried in soil. However, concern on the possibility of the pathogen survival urgently requires informative data on the buried host plants. Therefore, this study was conducted to investigate the survival of the pathogen from the buried host plants. METHODS AND RESULTS: Apple trees buried in 42 months ago in a Jecheon site and pear trees buried in 30 months ago in an Anseong site were excavated using an excavator. Plant samples were taken from stems and twigs of the excavated trees. The collected 120 samples were checked for rotting and used for bacterial isolation, using TSA, R2A, and E. amylovora selection media. The purely isolated bacteria were identified based on colony morphology and 16S rDNA sequences. Wood rotting and decay with off smells and discoloring were observed from the samples. A total of 17 genera and 48 species of bacteria were identified but E. amylovora was not detected. CONCLUSION: Our investigation suggests that the survival of E. amylovora doesn't seem possible in the infected hosts which have been buried in soil for at least 30 months. Therefore, the burial control can be considered as a safe method for fire blight disease.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.180-187
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• 2012

Panicum maximum, Panicum repens, Imperata cylindrica, Pennisetum polystachion and Cyperus rotundus are five rhizomatous grass weeds that exist and are problematic in the coconut lands of Sri Lanka. Rhizomatous weeds are the most difficult to control because of their vegetative reproduction by underground propagules. Therefore chemical and biological techniques have failed to control these weeds to acceptable levels. Experiments were conducted to investigate the impact of depth of burial, duration of sun drying or duration of air drying rhizomes of the selected weed species on germination. Depth of burial reduced germination of all species. Burying rhizomes at depths less than 10 cm inhibited germination of P. maximum and P. polystachion. Burying depths below 30~40 cm inhibited germination of all species. Sun drying or air drying rhizomes for durations less than 5 days inhibited germination of P. maximum and P. polystachion. Germinations of all species were inhibited by sun drying rhizomes for 15 days or air drying rhizomes for 20 days. Sun drying of rhizomes of all species for five days reduced the moisture content to a greater extent than air drying. The results indicated that burying rhizomes at the depthsbelow 30~40 cm, sun drying rhizomes for durations beyond 15 days or air drying rhizomes for durations beyond 20 days would be effective in controlling germination of these species. The investigations of the experiments also suggest that keeping rhizomes on the soil surface without burying, sun drying rhizomes or air drying rhizomes for durations of 5~15 days would produce weak plants.

• Korean Journal of Weed Science
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• v.30 no.2
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• pp.94-102
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• 2010

Field and pot experiments were conducted to investigate seedling emergence and early growth of Eleocharis kuroguwai panted on different dates. Non-linear regression analyses of observed data against effective accumulated temperature (EAT) with the Gompertz model showed that the Gompertz model works well in describing seedling emergence and early growth of E. kuroguwai regardless of planting date and soil burial depth. EATs required for 50% of the maximum seedling emergence of E. kuroguwai planted at 1, 3 and 5 cm soil burial depth in the pot experiment were estimated to be 54.5, 84.0 and $118.0^{\circ}C$, respectively, and $56.7^{\circ}C$ when planted at 1 cm in the field experiment. EATs required for 50% of the maximum leaf number of E. kuroguwai planted at 1, 3 and 5 cm soil burial depth in the pot experiment were estimated to be 213.3, 249.0 and $291.6^{\circ}C$, respectively, and $239.5^{\circ}C$ when planted at 1 cm in the field experiment. Therefore, models developed in this study thus predicted that if rotary tillage with water is made on 27 May under $+2^{\circ}C$ elevated temperature condition, dates for 50% of the maximum seedling emergence, 5 leaf stage and 5 cm plant height of E. kuroguwai buried at 3 cm soil depth were predicted to be 2 June, 10 June and 12 June. These dates are 1 day earlier for the seedling emergence and 3 days earlier for the early growth as compared with current temperature condition, suggesting that earlier application of herbicides is required for effective control of E. kuroguwai.

• Korean Journal of Weed Science
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• v.18 no.4
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• pp.295-303
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• 1998

The seed dormancy is one of the peculiar characteristics of a number of weed species and it makes difficulties in weed control. To clarify the mechanism of seed dormancy, several chemicals such as $KNO_3$, KOH, thiourea, and $H_2O_2$ and phytohormone($GA_3$) were treated to dormant seeds. Among the species treated with several chemicals, the germination percentages of Setaria glauca, Ambrosia trifida and Ranunculus sceleratus were increased with $KNO_3$ and those of S. glauca, R sceleratus were increased with thiourea. Hydrogen peroxide promoted the germination of Setaria viridis and S. glauca. Germination percentages of S. viridis, S. glauca and Cyperus saraguinolentus were increased with enzyme treatment using pectinase. GA treatment enhanced the geim.ination of Eleusine indica and R sceleratus but the other species were affected slightly. Especially. E. indica showed linearity in the relationship between germination percentage and GA concentration. So, It seemed that E. indica can be used as a bioassy material for GA. Considering the phenological habits of weed species, the seeds were buried under soil for long time(more than 1 month) over winter. When seeds were buried in soil, the degree of dormancy was drastically decreased. Especially, germination of seeds buried were increased under alternating temperature. The germination rates of Persicaria ssp. and Chenopodium ssp. were increased by 50% order alternating temperature after burial for seven weeks.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1404-1408
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• 2016

In recent years, foot-and-mouth disease has occurred in all parts of the world. The animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. Moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans, livestock, and the environment from being affected with the disease. The treatment of Corynebacterium glutamicum is a feasible method to reduce the risk of carcass decomposition affecting humans or the environment. Therefore, this study aimed to investigate the effect of C. glutamicum on the soil environment with a carcass. The composition of amino acids in the soil treated with C. glutamicum was generally higher than those in the untreated soil. Moreover, the plant root in the soil samples treated with C. glutamicum had 84.0% amino acids relative to the standard value and was similar to that of the control. The results of this study suggest the possibility to reduce the toxicity of a grave land containing animals with this disease.

• Korean Journal of Heritage: History & Science
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• v.41 no.1
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• pp.99-108
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• 2008

The paleogenetic analysis has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. Recently, scientific research for human skeletal remains was more activated because of increasing awareness of the valuable archaeological information by the ancient DNA analysis. State of preservation of organic remains vary in different soil and burying environmental condition. Almost all available tissue disappear to analysis ancient DNA of bone in acidic soil caused by climate and geological features in Korea. Many preserved human remains excavated in the 'Heogwakmyo'(limelayered tomb of Chosun Dynasty Period) is able to explain through the relationship between burial conditions and bone survival form the burial method and ceremony. Ancient DNA analysis of excavated human bone form ancient tomb requires to remove contaminants such as microorganism's DNA and soil components that affect authentic results. Particularly, contamination control of contemporary human DNA is major serious problem and should verified by criteria of authenticity. In order to understand migration and culture of ancient population, when possible, ancient DNA studies needs to go abreast both radiocarbon and stable isotope studies because the dietary inferences will suggest ancient subsistence and settlement patterns. Also when the paleogenetic research supported with the arts and humanities research such as physical anthropology and archaeology, more valuable ancient genetic information is providing a unique results about evolutionary and population genetics studies to reconstruct the past.