• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.815-820
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• 2008

Two culture-independent methods, namely ribosomal DNA libraries and denaturing gradient gel electrophoresis (DGGE), were adopted to examine the microbial community of a Malaysian light crude oil. In this study, both 16S and 18S rDNAs were PCR-amplified from bulk DNA of crude oil samples, cloned, and sequenced. Analyses of restriction fragment length polymorphism (RFLP) and phylogenetics clustered the 16S and 18S rDNA sequences into seven and six groups, respectively. The ribosomal DNA sequences obtained showed sequence similarity between 90 to 100% to those available in the GenBank database. The closest relatives documented for the 16S rDNAs include member species of Thermoincola and Rhodopseudomonas, whereas the closest fungal relatives include Acremonium, Ceriporiopsis, Xeromyces, Lecythophora, and Candida. Others were affiliated to uncultured bacteria and uncultured ascomycete. The 16S rDNA library demonstrated predomination by a single uncultured bacterial type by >80% relative abundance. The predomination was confirmed by DGGE analysis.

• Journal of information and communication convergence engineering
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• v.3 no.1
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• pp.23-27
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• 2005

In this paper, the effects of various annealing conditions on the resonance characteristics of ZnO-based FBAR devices are compared. Several FBAR device samples were fabricated by using three different annealing methods while one sample remained non-annealed as a reference for comparison. Return loss ($S_11$) could be significantly improved by both Bragg reflector-annealing and/or post-annealing. Especially, the use of the combined annealings resulted in most desirable resonance improvement compared with the Bragg reflector-annealing or post-annealing method alone.

• Journal of the Korean Magnetics Society
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• v.5 no.5
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• pp.745-748
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• 1995

Magnetic nanoparticles of iron and iron oxide have been prepared in a modified upward thermal diffusion cloud chamber using pulsed laser evaporation. SEM/TEM studies of these particles reveal a size distribution with a mean diameter of about $60\;{\AA}$. FTIR spectrum measurements are used to investigate the difference in oxidation level between nanoparticles prepared at different partial oxygen pressures. The complex magnetic behaviour of these particles was studied using DC- and AC-susceptibility measurements. All samples exhibit superparamagnetism with blocking temperatures ranging from 50 K to above room temperature. The coercivity fields as well as the dependence of the blocking temperature on measuring frequency have been studied. magnetic anisotropy constants are found to be one order of magnitude higher than is known for the bulk values. The mean particle size estimated from the magnetic data is found to be in perfect agreement with the TEM observations.

• Genomics & Informatics
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• v.20 no.1
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• pp.13.1-13.4
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• 2022

A major issue in the use of immune checkpoint inhibitors is their lack of efficacy in many patients. Previous studies have reported that the T cell inflamed signature can help predict the response to immunotherapy. Thus, many studies have investigated mechanisms of immunotherapy resistance by defining the tumor microenvironment based on T cell inflamed and non-T cell inflamed subsets. Although methods of calculating T cell inflamed subsets have been developed, valid screening tools for distinguishing T cell inflamed from non-T cell inflamed subsets using gene expression data are still needed, since general researchers who are unfamiliar with the details of the equations can experience difficulties using extant scoring formulas to conduct analyses. Thus, we introduce TcellInflamedDetector, an R package for distinguishing T cell inflamed from non-T cell inflamed samples using cancer gene expression data via bulk RNA sequencing.

• Progress in Superconductivity and Cryogenics
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• v.24 no.4
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• pp.55-58
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• 2022

Carbon-based doping to MgB₂ superconductor is the simplest approach to enhance the critical current densities under magnetic fields. Carbon quantum dots is synthesized in this work as a carbon provider to MgB₂ superconductors. Polyvinyl Pyrrolidone is pyrolyzed and dispersed in dimethylfomamide solvent as a dopant to the mixture of Mg and B powders. Doped MgB₂ bulk samples clearly show the decrease of a-axis lattice constant, grain refinements, and broadening of FWHM of diffraction peaks compared to un-doped MgB₂ possibly due to the carbon substitution and/or boron vacancy at the boron site in MgB₂ lattice. Also, high-field J_c for the doped MgB₂ is enhanced significantly with the crossover about 3 T at 5 & 20 K when increasing the doping of carbon quantum dots.

• Archives of Metallurgy and Materials
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• v.65 no.3
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• pp.1005-1009
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• 2020

In this study, a non-equiatomic high entropy alloy was fabricated using the spark plasma sintering method, and its microstructural features and mechanical properties were investigated. The chemical composition of FeMnCoCr was determined by using the entropy calculation related to the design of high entropy alloys. A bulk sample with the same composition was also prepared using the conventional metallurgical processes of casting and hot rolling. The microstructures of the samples fabricated by these different processes were compared by microscope observation, and a quantitative phase analysis was carried out using FE-SEM. Hardness measurement was used to evaluate mechanical properties. Particular attention was paid to microstructural changes due to heat treatment, which was analyzed by considering how austenite stability is affected by grain refinement.

• Economic and Environmental Geology
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• v.13 no.2
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• pp.69-79
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• 1980

Granitic rocks, from the Eonyang and the northwestern part of Ulsan area, were mainly studied from the petrochemical point of view. From field work, microscopic observation and the result of K-Ar ages, these granitic rocks are divided into six rock types of a, b, c, d, e and e', of which modal composition are given. Type a, c, e and e' are mainly granodiorite, type b is adamellite, and type d is granodiorite, adamellite and others (porphyritic rock, porphyry and felsitic rocks). Bulk chemical analyses of 22 samples of the granitic rocks are given. The petrographical and petrochemical characteristics of these rocks are discussed briefly. In the petrochemical compositions with their characteristic variation trends of several oxides and norm Or-Ab-An triangular diagram, type a, b and c show some similarity to that of San-yo granite of Japan and younger granite of Ogcheon geosynclinal zone, and then, type e and e', to San-in granite of Japan. But, some of granitic rock samples of type d are similar to San-yo granite and the others of type d to San-in granite because this type is composed of porphyritic rock, porphyry and felsitic rocks. According to the result of K-Ar ages (1976, Lee et al.) of rock samples from type a, b and care Cretaceous, on the otherwise, type d, e and e', are Tertiary granitic rocks. Judging from these evidences, granitic rocks in the area are correlated to the Cretaceous and Tertiary granitic rocks in the, southwestern Japan, that is, type a, band c are correlated to San-yo granite, and type d, e and e' to San-in granite.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.1
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• pp.43-48
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• 2008

Purpose: This study is to evaluate reliability of geometrical optics properties of spectacle lenses by using ISO and the medical instrument standard of KFDA, which are being sold in Korea. Methods: We used samples of three hundred and ninety eight spectacle lenses of eight company in total. Refractive indices of each samples which were used in experiment were classified into three groups of medium index (1.55~1.56), high index (1.60~1.61) and extra high index (1.67). Results: Conformity of refractive power was 81.61% in total spectacle lenses. The results showed that thickness conformity 90%, appearance conformity 85.18%, size conformity 96.23% and optical center point conformity 99.50% in total. Conclusions: We found that they deviated from the permitting value in many spectacle lenses on refractive power. The results of errors on prism power, surface inspection and optical center point showed small values in total products. In experiment of lens size and thickness, the bulk of indication rates and conformities of samples deviated from the permitting errors.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.4
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• pp.492-500
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• 2019

Equal channel angular pressing(ECAP) is a severe plastic deformation technique capable of introducing large shear strain in bulk metal materials. However, if an ECAPed material has an inhomogeneous microstructure and anisotropic mechanical properties, this material is difficult to apply as structural components subjected to multi-axial stress during use. In this study, extruded oxygen-free copper(OFC) rods with a large diameter of 42 mm are extruded through ECAP by route Bc up to 12 passes. The variations in the microstructure, hardness, tensile properties, and microstructural and mechanical homogeneity of the ECAPed samples are systematically analyzed. High-strength OFC rods with a homogeneous and equiaxed-ultrafine grain structure are obtained by the repeated application of ECAP up to 8 and 12 passes. ECAPed samples with 4 and 8 passes exhibit much smaller differences in terms of the average grain sizes on the cross-sectional area and the tensile strengths along the axial and circumferential directions, as compared to the samples with 1 and 2 passes. Therefore, it is considered that the OFC materials, which are fabricated via the ECAP process with pass numbers of a multiple of 4, are suitable to be applied as high-strength structural parts used under multi-axial stress conditions.

• Journal of Forest and Environmental Science
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• v.37 no.2
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• pp.116-127
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• 2021

Predicting carbon distribution of soil aggregates is difficult due to complexity in organo-mineral formation. This limits global warming mitigation through soil carbon sequestration. Therefore, knowledge of land use effect on carbon stabilization requires quantification of soil mineral cations. The study was conducted to quantify carbon and base cations on soil mineral fractions in Natural Forest, Plantation Forest and Farm Land. Five 0.09 ha were demarcated alternately along 500 m long transect with an interval of 50 m in Natural Forest (NF), Plantation Forest (PF) and Farm Land (FL). Soil samples were collected with soil cores at 0-15, 15-30 and 30-45 cm depths in each plot. Soil core samples were oven-dried at 105℃ and soil bulk densities were computed. Sample (100 g) of each soil core was separated into >2.0, 2.0-1.0, 1.0-0.5, 0.5-0.05 and <0.05 mm aggregates using dry sieve procedure and proportion determined. Carbon concentration of soil aggregates was determined using Loss-on-ignition method. Mineral fractions of soil depths were obtained using dispersion, sequential extraction and sedimentation methods of composite soil samples and sieved into <0.05 and >0.05 mm fractions. Cation exchange capacity of two mineral fractions was measured using spectrophotometry method. Data collected were analysed using descriptive and ANOVA at α_0.05. Silt and sand particle size decreased while clay increased with increase in soil depth in NF and PF. Subsoil depth contained highest carbon stock in the PF. Carbon concentration increased with decrease in aggregate size in soil depths of NF and FL. Micro- (1-0.5, 0.5-0.05 and <0.05 mm) and macro-aggregates (>2.0 and 2-1.0 mm) were saturated with soil carbon in NF and FL, respectively. Cation exchange capacity of <0.05 mm was higher than >0.05 mm in soil depths of PF and FL. Fine silt (<0.05 mm) determine the cation exchange capacity in soil depths. Land use and mineral size influence the carbon and cation exchange capacity of Gambari Forest Reserve.