• Title/Summary/Keyword: Budding

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A Study on Lateral Torsional Budding of Arch Subjected to Pure Bending Moment (순수 휨모멘트를 받는 아치의 횡좌굴에 관한 연구)

  • Kim, Saeng Bin;Yoo, Chai Hong;Lee, Sung Chul
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.9 no.3
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    • pp.13-19
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    • 1989
  • A system of coupled differential equations governing the lateral-torsional buckling of thin-walled arches subjected to pure bending moment is presented. The governing differential equations are derived using incremental form of principle of virtual displacement based on updated Lagrangian procedure. The differential equations are solved for the critical end moments of arches with I section, and then comparative studies are made with existing solutions.

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Effects of the Early Soil Moisture Content on the Growth and Chemical Components of Tobacco (Nicotiana tabacum L.) (이식초기 토양수분차이가 담배의 생육 및 내용성분에 미치는 영향)

  • 한종구;윤병익;반유선;손응룡
    • Journal of the Korean Society of Tobacco Science
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    • v.3 no.2
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    • pp.103-108
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    • 1981
  • This experiment was carried out to investigate effects of soil moisture content on the growth of tobacco plant at early stage after transplanting. Soil moisture was controlled to be 30%, 45%, 60%, 75% and 90% of the maximum water holding capacity (38.7%), and treated for 10, 20 and 30 days. Budding flowering and topping were delayed in the 30% and 45% treatment where soil moisture was deficient. Plant height, number of leaves, and length and width of the largest leaf were the best in the 75% treatment for 10 days, and development of the root and top was the best also in the same treatment. As the duration of low soil moisture treatment prolonged, intercellular space , became small. Nitrogen and potassium of the cured leaf showed the highest value in 30% and 45% treatments. Nicotine content of the cured leaf was high in the 90% treatment, and reducing sugar content of that was high in the 75% treatment for 10 days.

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Transcriptional Response According to Strength of Calorie Restriction in Saccharomyces cerevisiae

  • Lee, Yae-Lim;Lee, Cheol-Koo
    • Molecules and Cells
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    • v.26 no.3
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    • pp.299-307
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    • 2008
  • To characterize gene expression that is dependent on the strength of calorie restriction (CR), we obtained transcriptome at different levels of glucose, which is a major energy and carbon source for budding yeast. To faithfully mimic mammalian CR in yeast culture, we reconstituted and grew seeding yeast cells in fresh 2% YPD media before inoculating into 2%, 1%, 0.5% and 0.25% YPD media to reflect different CR strengths. We collected and characterized 160 genes that responded to CR strength based on the rigorous statistical analyses of multiple test corrected ANOVA (adjusted p value < 0.1 or raw p value < 0.0031) and Pearson correlation (|r| > 0.7). Based on the individual gene studies and the GO Term Finder analysis of 160 genes, we found that CR dose-dependently and gradually increased mitochondrial function at the transcriptional level. Therefore, we suggest these 160 genes are markers that respond to CR strength and that might be useful in elucidating CR mechanisms, especially how stronger CR extends life span more.

Effect of lateral restraint on the buckling behaviour of plates under non-uniform edge compression

  • Bedair, Osama K.
    • Structural Engineering and Mechanics
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    • v.5 no.1
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    • pp.85-104
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    • 1997
  • The paper investigates the influence of lateral restraint on the buckling behaviour of plate under non-uniform compression. The unloaded edges are assumed to be partially restrained against translation in the plane of the plate and the distributions of the resulting forces acting on the plate are shown. The stability analysis is done numerically using the Galerkin method and various strategies the economize the numerical implementation are presented. Results are obtained showing the variation of the buckling load, from free edge translation to fully restrained, with unloaded edges simply supported, clamped and partially restrained against rotation for various plate aspect ratios and stress gradient coefficients. An apparent decrease in the buckling load is observed due to these destabilizing forces acting in the plate and changes in the buckling modes are observed by increasing the intensity of the lateral restraint. A comparison is made between the budding loads predicted from various formulas in stability standards based on free edge translation and the values derived from the present investigation. A difference of about 34% in the predicted buckling load and different buckling mode were found.

Msi1-Like (MSIL) Proteins in Fungi

  • Yang, Dong-Hoon;Maeng, Shinae;Bahn, Yong-Sun
    • Mycobiology
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    • v.41 no.1
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    • pp.1-12
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    • 2013
  • Msi1-like (MSIL) proteins, which are eukaryote-specific and contain a series of WD40 repeats, have pleiotropic roles in chromatin assembly, DNA damage repair, and regulation of nutrient/stress-sensing signaling pathways. In the fungal kingdom, the functions of MSIL proteins have been studied most intensively in the budding yeast model Saccharomyces cerevisiae, an ascomycete. Yet their functions are largely unknown in other fungi. Recently, an MSIL protein, Msl1, was discovered and functionally characterized in the pathogenic yeast Cryptococcus neoformans, a basidiomycete. Interestingly, MSIL proteins appear to have redundant and unique roles in both fungi, suggesting that MSIL proteins may have evolutionarily divergent roles in different parts of the fungal kingdom. In this review, we will describe the current findings regarding the role of MSIL proteins in fungi and discuss future directions for research on this topic.

Effects of Phytohormones on the Viability and Cell Wall Regeneraton of Tobacco Protoplasts (연초 원형질체의 생존율과 세포벽 재생에 미치는 식물생장조절물질의 효과)

  • 김용옥
    • Journal of Plant Biology
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    • v.31 no.2
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    • pp.121-130
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    • 1988
  • In order to clarify effects of phytohormones on the viability and the cell wall regeneration of protoplasts isolated from Nicotiana tobacum L. var. BY4, protoplasts isolated from mesophyll tissue were cultured on the Murashige-Skoog liquid media supplemented with auxin(2, 4-D, NAA, IAA) and/or cytokinin (kinetin, BAP, 2ip). Viability of protopplasts was higher in the culture medium containing auxin and cytokinin, especially in the combination of 2, 4-D and BAP. The effectual cell wall regeneration of protolasts was observed when theprotoplasts were cultrued on the medium supplemented with auxin alone, especially with IAA. Cell wall regernation started from 2-3 days after culture and was not detected at budding regions. When the protoplasts were cultured on the phytohormone-free medium, the viability of protoplasts dramatically decreased 4 days after culture.

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A Study on the Geometrically Nonlinear Analysis of Spatial Structures by Using Arc Length Method (호장법을 이용한 공간구조의 기하학적 비선형 해석에 관한 연구)

  • Han, Sang-Eul;Lee, Sang-Ju;Lee, Kyoung-Soo
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2007.04a
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    • pp.381-386
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    • 2007
  • The present study is concerned with the application of Constant arc-length method that proposed by Crisfield in the investigation of the geometrically nonlinear behaviour of spatial structures composed by truss or beam element. The arc-length method can trace the full nonlinear equilibrium path of Spatial structure far beyond the critical point such as limit or bifurcation point. So, we have developed the constant arc-length method of Crisfield to analysis spatial structure. The finite element formulation is used to develop the 3d truss/beam element including the geometrical nonlinear effect. In an effort to evaluate the merits of the methods, extensive numerical studies were carried out on a number of selected structural systems. The advantages of Constant arc length method in tracing the post-buckling behavior of spatial structures, are demonstrated.

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Evaluation of Effective Length Factor by Using an Amplification Factor (확장계수를 적응한 기둥의 유효좌굴길이 계수 산정)

  • Choi, Dong-Ho;Yoo, Hoon;Shin, Jay-In;Kim, Sung-Yeon
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2007.04a
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    • pp.369-374
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    • 2007
  • For a stability design of steel frames, AISC-LRFD specification recommend to use Alignment Chart and story-based methods in order to determine an effective budding length. Recently, elastic buckling analysis, which is the method that calculate the effective length of members using eigenvalue of the overall structure, has been widely used in practical design of steel frames because this method can be performed effectively and automatically by computers. However, it can in some cases lead to unexpectedly large effective length in column having small axial forces. Therefore, this paper propose a method using elastic buckling analysis, which estimate a proper effective buckling length for all members having a small axial force. For verification of proposed method, it is compared with system based approach and stiffness distribution factor method. As a result, proposed method can rationally solve a problem in some case of column having small axial force. Also, adoption range for proposed method is established.

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Role of CaBud6p in the Polarized Growth of Candida albicans

  • Song Yun-Kyoung;Kim Jeong-Yoon
    • Journal of Microbiology
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    • v.44 no.3
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    • pp.311-319
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    • 2006
  • Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candide albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-Hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.

Vegetative and Male Reproductive Anatomy of Laurencia intercalaris sp. nov. (Rhodomelaceae, Rhodophyta) in Korea

  • Nam, Ki-Wan
    • Journal of Plant Biology
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    • v.37 no.4
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    • pp.421-428
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    • 1994
  • The vegetative and male reproductive anatomy of a marine alga, Laurencia intercalaris sp. nov. (Rhodomelaceae, Rhodophyta), is described from subtidal habitats of eastern and southern Korea. This species has terete thalli with entangled fibrous holdfasts and regularly alternate branching of ultimate branchlets, and is inseparable from L. okamurae Yamada on the basis of habit. Vegetative axial cells produce a trichoblast and four pericentral cells in an alternating sequence. Spermatangia are produced intercalary or subterminally from one of two laterals on suprabasal cells of trichoblasts arising from axial cells in apical pits of branchlets. The other lateral remains sterile. In this sterile lateral, budding-like regeneration occurs on older segments that are oabscised. Comparison is made with other related Laurencia species, particularly those with terete thalli. The vegetative anatomy and the regeneration in sterile laterals of male trichoblasts, with the mode of spermatangial formation, distinguish the new species from previously described species of Laurencia including L. okamurae.

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