• Natural Product Sciences
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• v.27 no.3
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• pp.176-182
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• 2021

The phytochemical investigation of Broussonetia kazinoki roots led to the isolation of ten compounds, including six flavonoids (1-6), two lignans (7 and 8), and two coumarins (9 and 10) by comparing their ¹H and ¹³C NMR spectra with reference values. To the best of our knowledge, compounds 9 and 10 were isolated from this plant for the first time. Among the ten isolates, compounds 2, 4, and 6 exhibited inhibitory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW264.7 cells with IC₅₀ values of 11.98, 10.16, and 24.06 μM, respectively. Furthermore, compounds 2, 4, and 6 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a dose-dependent manner. Pre-incubation of cells with these compounds also significantly suppressed LPS-induced COX-2 protein expression. Compounds 2, 4, and 6 also showed cytotoxic activity against HL-60 cells with IC₅₀ values ranging between 46.43 and 94.06 μM.

• Biomolecules & Therapeutics
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• v.22 no.5
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• pp.438-444
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• 2014

Broussonetia kazinoki Siebold. (B. kazinoki) has long been used in the manufacture of paper in Asian countries. Although B. kazinoki leaves (BK) have been employed in dermatological therapy, use of BK has not been tested in patients with atopic dermatitis (AD). Using Nc/Nga mice, which are genetically predisposed to develop AD-like skin lesions, we confirmed the efficacy of BK in AD treatment. BK extract was applied topically to Dermatophagoides farinae-induced AD-like lesions in Nc/Nga mice, and the effects were assessed both clinically and by measuring skin thickness on the back and ears. We measured the effects of BK extract on plasma levels of IgE and IL-4. We also measured the ability of BK extract to inhibit the secretion of hTARC in HaCaT cells after stimulation by TNF-${\alpha}$ and IFN-${\gamma}$. We found that BK extract significantly reduced ear and dorsal skin thickness and the clinical signs of AD, as well as significantly down-regulating the plasma levels of IgE and IL-4 (p<0.01 for each comparison). Moreover, $500{\mu}g/mL$ of BK extract inhibited hTARC secretion in HaCaT cells by activated TNF-${\alpha}$/IFN-${\gamma}$ by about 87%. These findings suggest that topical application of BK extract has excellent potential in the treatment of AD.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.35-41
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• 2012

Nondestructive evaluation (NDE) technique method using a resonance frequency mode was carried out for woodceramics made by different carbonizing temperature (600, 800, 1000, $1200^{\circ}C$) for Broussonetia kazinoki Sieb. Dynamic modulus of elasticity increased with increasing carbonizing temperature. There was a close relationship of dynamic modulus of elasticity and static bending modulus of elasticity to MOR. Therefore, the dynamic modulus of elasticity using resonance frequency mode is useful as a nondestructive evaluation method for predicting the MOR of woodceramics made by different carbonizing temperature for B. kazinoki Sieb.

• Korean journal of food and cookery science
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• v.10 no.4
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• pp.346-350
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• 1994

This investigation was undertaken for the purpose of studying the effect of the addition of the powder(0, 1, 2, 3%)from fruit of paper mulberry(Broussonetia Kazinoki SieboId) on the hardness, cohesiveness, elasticity, gumminess, chewiness, cooking loss and sensory characteristics of cooked ground beef with or without soysauce. The results obtained were summerized as follows; Hardness, gumminess, chewiness, and cooking loss were decreased by increasing the percent of adding the powder from the fruit of paper mulberry. As a result of sensory evaluation for cooked ground beef, sample with powder from the fruit of paper mulberry showed the higher scores in most of the characteristics. And cooked ground beef added the powder from the fruit of paper mulberry with soysauce showed the higher decreasing rate of hardness than those of without soysauce.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.31 no.3
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• pp.83-89
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• 1999

Bast fibers of paper mulberry ( Broussonetia Kazinoki Sieb) were cooked with a weak alkaline salt, potassium carbonate which has been known as a major inorganic component of the traditional lye, and its cooking characteristics were investigated . The bast fiber was easily cooked by potassium carbonate. The pulp yield was rapidly decreased up to 20-30 mol of potassium carbonate, but the Kappa number was slowly decreased with increasing of potassium carbonate. The potassium carbonate pulps were easily defibered at low cooking chemical charge of 25mm on and high pulp yield of about 80%. These results were confirmed that pectin was easily removed during the potassium carbonate cooking. In contrast, when sodium carbonate was used as a cooling agent, the bast fiber was only partially defibered. Thus, sodium carbonate was a less effective cooling chemical of the bast fiber. The results of this experiment indicated that potassium carbonate could not only be used as a good cooling agent of bast fiber, but also as an alternative agent of sodium hydroxide.

• Applied Chemistry for Engineering
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• v.24 no.2
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• pp.177-183
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• 2013

In this study, the antioxidative activities and component analysis of Broussonetia kazinoki SIEB (B. kazinoki). extracts were investigated. B. kazinoki extract showed the effective free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}=8.53{\mu}g/mL$). Reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) of the ethyl acetate fraction of B. kazinoki. extracts in the luminol-dependent $Fe^{3+}-EDTA/H_2O_2$ system was $1.69{\mu}g/mL$. The ethyl acetate fraction of B. kazinoki. extracts also exhibited more prominent cellular protective effects (${\tau}_{50}$, 183.3 min at $10{\mu}g/mL$) than that of typical antioxidant $\alpha$-tocopherol (${\tau}_{50}$ = 38.00 min) in the $^1O_2$-induced photohemolysis of human erythrocytes. Components of the ethyl acetate fraction obtained from B. kazinoki extracts were analyzed by TLC, HPLC chromatogram, LC/ESI-MS/MS and $^1H$-NMR. Consequently, Components, components were identified as the kazinol J of kazinol series and luteolin (2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) of flavonoid series having antioxidant activities. These results indicate that extract/ fraction of B. kazinoki can be used as antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging $^1O_2$ and other ROS, and protecting cellular membranes against ROS. Thus, the extract/fraction of B. kazinoki could be applicable to new cosmeceuticals.

• The Journal of Korean Medicine
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• v.32 no.3
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• pp.10-17
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• 2011

Objective: To evaluate the immune-stimulatory potential of extracts of Broussonetia kazinoki Siebold (BK) on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum ($100{\mu}g/200{\mu}g$) on days 1, 8, and 15. BK (100, 300 or 1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis. Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN-${\gamma}$ in splenocytes. Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.

• Journal of agriculture & life science
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• v.44 no.4
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• pp.21-27
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• 2010

This study was carried out to investigate the properties of woodceramics made from woody part of Broussonetia Kazinoki at different carbonizing temperatures of $600^{\circ}C$, $800^{\circ}C$, $1000^{\circ}C$and $1200^{\circ}C$. The physical and mechanical properties increased with increasing carbonizing temperature. The highest mean values of density, bending strength, Brinell hardness and compressive strength were $0.62g/cm^3$, $79kgf/cm^2$, $203kgf/cm^2$ and $129kgf/cm^2$, respectively. There were close correlations between density and bending strength, Brinell hardness and compressive strength and MOE and MOR.

• Korean Journal of Plant Resources
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• v.35 no.1
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• pp.36-43
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• 2022

This research was conducted to develop a seed propagation protocol for the use of Broussonetia kazinoki Siebold ex Siebold & Zucc, which native to East Asia. Light and temperature conditions, GA₃ treatment, and low-temperature stratification were performed to evaluate the germination characteristics. The germination percentage at 4, 15/6, 20/10 and 25/15℃ was 0, 42. 5, 44. 4 and 91.7%, respectively. Thus, the optimal germination temperature of the B. kazinoki seeds was 25/15℃. Although germination was delayed by relatively low temperature conditions, GA3 treatment allowed for increased germination rates even under the low temperature conditions. Cold stratification reduced time to germination. Relatively low germination percentages at 20/10℃ and 15/6℃ indicated that the seeds had been under the state of conditional dormancy. Through this study, B. kazinoki seeds have conditional dormancy because they can be germinated through GA₃ treatment even under relatively low temperature conditions and it is judged that it will support the commercial propagation practice in the future.

• Herbal Formula Science
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• v.27 no.3
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• pp.223-236
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• 2019

Dengropanax morfiferus (D), Broussonitia kazinoki (B), and Cudriania tricuspidata (E), a widely cultivated species in South Korea, has been used as traditional medicine to treat numerous diseases. In this study, we evaluated the antidiabetic effects in a various signaling mechanisms using mixed extract and major component contents were analyzed by HPLC in the combined extracts from Dengropanax morfiferus, Broussonitia kazinoki, and Cudriania tricuspidata (DBCE). DBCE inhibited ${\alpha}$-glucosidase and ${\alpha}$-amylase activation and showed potent antioxidant effects, which are evaluated using DPPH, ABTS, and SOD assay. Cytokines, which are released by inflammatory cells in pancreatic islets, are involved in the pathogenesis of type 1 diabetes mellitus. DBCE showed the protective effects in RINm5F cells against cytokines-induced damage by suppressing inducible nitric oxide (NO) synthase and COX-2 expression and NO production. Insulin resistance is the primary characteristic of type 2 diabetes. Therefore, the regulatory effect of DBCE on glucose uptake and production are investigated in insulin-responsive human HepG2 cells. DBCE stimulated glucose uptake, prevented Glut2 and phosphor-IRS1 downregulation induced by high glucose (HG, 30 mM). Moreover, DBCE pretreatment diminished glucose levels, PEPCK and G6Pase overexpression provoked by HG. These findings suggest that DBCE might be used for diabetes treatment through alpha-glucosidase or alpha-amylase activity regulation, pancreatic beta cell protection, hepatic glucose sensitivity improvement. Cytokines, which are released by inflammatory cells' infiltrations around the pancreatic islets, are involved in the pathogenesis of type 1 diabetes mellitus.