The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Broussonetia kazinoki Siebold stimulates immune response in ovalbumin-immunized mice

  • Jung, Da-Young (Herbal Medicine EBM Research center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine EBM Research center, Korea Institute of Oriental Medicine) ;
  • Lee, Jin-Ah (Herbal Medicine EBM Research center, Korea Institute of Oriental Medicine) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Choi, Young-Jae (ChunYang Paper Co., Ltd.) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research center, Korea Institute of Oriental Medicine)
  • Received : 2011.02.11
  • Accepted : 2011.04.22
  • Published : 2011.05.30
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Abstract

Objective: To evaluate the immune-stimulatory potential of extracts of Broussonetia kazinoki Siebold (BK) on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum ($100{\mu}g/200{\mu}g$) on days 1, 8, and 15. BK (100, 300 or 1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis. Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN-${\gamma}$ in splenocytes. Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.

Keywords

References

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