• Title/Summary/Keyword: Breeding information

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Studies on Tissue Culute in Rice, Oryza sativa L. (벼 조유배양에 관한 연구)

  • Gun-Sik Chung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.20
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    • pp.1-26
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    • 1975
  • In order to provide basic information for the application of tissue culture to a rice breeding program. experiments were carried out on the callus formation from seedings and anthers and organ differentiation from the callus of rice varieties and their hybrids. It appeared that in general the callus tissue was more easily induced in japonicas than in indicas with significant varietal differences. A highly significant positive correlation (r=0.896$^{**}$) was obtained between the fresh weight of callus induced in NAA medium and in 2, 4-D medium in the same variety. Callus formed on the medium with 2, 4-D was more friable than that formed on the medium with NAA. The callus formation from seedlings was better than from anthers in upland rice varieties. The easiness of the callus formation appeared to be a dominant character in crosses of rice varieties. Varietal difference was also noticed in the organ differentiation from the callus and the root differentiation seemed to be easier than the shoot differentiation. Most of the plants derived from the cal1us were albinos and the frequency of occurrence of albinos was greater in callus of seedlings than in that of anthers. Greater amount of callus tissue was formed from anthers of $F_1$ plants than anyone of their parents in remote-crosses but it was clear in close-crosses. A highly significant positive correlation (r=0.504$^{**}$) was found between the callus formation from seedlings and from anthers.

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Studies on Varietal Resistance to Sheath Blight Disease in Rice III. Variation of Varietal Resistance by Inoculating Different Fungus Isolates (벼 품종의 잎집무늬마름병 저항성연구 III. 접종균주에 따른 품종저항성의 변이)

  • Kwang-Ho Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.309-314
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    • 1988
  • This study was carried out to get information for the breeding varieties and developing the cultural methods with representative 32 cultivars which were diffused from 1900 to 1982 and two cultural methods which were old one i.e., low dressing and late planting and modern one i.e., medium dressing and early planting. Duration of transplanting between cultural practices were 15 days but the duration of heading date were 5 days. Panicle projecting duration was 5-10 days in all cultivars, especially, it is 5 days in early maturing varieties, 6-7 days in medium maturing varieties and 7-10 days in late maturing varieties. Periods from transplanting date to effective ripening date were 99.8 days in low dressing and late planting method, 106.2 days in modern one but the periods from heading date to effective ripening date were 31.4 days in low dressing and late planting method, 30.2 days in modern one. Ripening speed was very active from 5 to 10 days after heading and the daily increase of 1000 grain weight was from 847.8mg to 1130mg in that time and it slightly increased from 5 to 35 days after heading in japonica varieties but increased after heading and suddenly decreased at 30 days after heading in Indica/Japonica varieties. Meteorological factors (temperature and amount of solar radiation) were positively correlated with the ripening speed but negatively correlated with the period of physiological and effective ripening.

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Interitance of Pericarp Thickness of Waxy Maize (찰 옥수수 과피두께의 유전)

  • Lee, In-Sup;Choe, Bong-Ho;Lee, Won-Koo;Lee, Hee-Bong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.6
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    • pp.489-494
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    • 1993
  • The amount of maize being imported from other countries to meet the national demand are increasing every year. Regardless of the usage whether it is for silage or for human consumption, amount of seeds for farmers are ever being increased. In order to solve the problems arising from the seed import, a program for developing waxy hybrids with high quality was set up at the College of Agr., Chungnam National University. The main breeding targets for high quality waxy hybrids are focused on the pericarp thickness. In order to obtain basic information needed for developing hybrids with thin pericarp, six inbred lines all derived from open pollinated Korean waxy lines were diallel crossed. Results obtained indicate that waxy hybrids with thin pericarp can be developed by choosing proper parental lines. Of the six inbreds, Jewon inbred had utmost thin pericarp compared with other lines. Hybrids crossed with Jewon showed also thinner pericarp than other hybrids. However, Danyang which has thick pericarp showed thicker pericarp in hybrid combinations. Variance due to general combining abilities was greater than the variance due to the specific combining abilities, indicating that additive gene effects are more important. The pericarp thickness of waxy hybrid endosperm varied with the parts of pericarp. The germinal side of the pericarp is comparatively thinner than the abgerminal side. The upper part (crown) has thicker pericaip than lower part (tip) of the kernel.

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Analysis of Phenolic Compounds in Sorghum, Foxtail Millet and Common Millet

  • Jeon, Hyun-Seok;Chung, Ill-Min;Ma, Kyung-Ho;Kim, Eun-Hye;Yong, Soo-Jung;Ahn, Joung-Kuk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.4
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    • pp.361-374
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    • 2011
  • The cereal grain crops have strong flexibility against adverse environment and they have various functional compounds. The objective of the present study was to screen phenolic compounds in sorghum [Sorghum bicolor (L.) Moench], foxtail millet (Setaria italica), common millet (Panicum miliaceum L.) by high performance liquid chromatography (HPLC) with photodiode array (PDA) detector. Sorghum contained the highest amount of phenolic compounds among three different crops (sorghum, foxtail millet, common millet). Especially Moktaksusu showed the highest amount of phenolic compounds concentrations and biggest regional differences. The comparison of average phenolic compounds in sorghums by regions showed order to Milyang ($963.3\;{\mu}g{\cdot}g^{-1}$), Yeongyang ($923.1\;{\mu}g{\cdot}g^{-1}$), Gijang ($831.3\;{\mu}g{\cdot}g^{-1}$) and Bonghwa ($735.6\;{\mu}g{\cdot}g^{-1}$). Among the sorghum cultivars, Moktaksusu ($1407.9\;{\mu}g{\cdot}g^{-1}$) had the highest concentration of phenolic compounds. The average phenolic compounds of foxtail millets showed similar amount among Milyang ($319.0\;{\mu}g{\cdot}g^{-1}$), Gijang ($288.1\;{\mu}g{\cdot}g^{-1}$) and Bonghwa ($281.9\;{\mu}g{\cdot}g^{-1}$) areas. The phenolic compounds of Yeongyang ($246.6\;{\mu}g{\cdot}g^{-1}$) slightly low and that showed similar concentrations among three different regions. The concentration of phenolic compounds in foxtail millets, Chungchajo ($335.6\;{\mu}g{\cdot}g^{-1}$) showed the highest concentrations. The average phenolic compounds of common millets showed the highest concentrations in Milyang ($305.5\;{\mu}g{\cdot}g^{-1}$), Bonghwa ($262.0\;{\mu}g{\cdot}g^{-1}$), Gijang ($195.1\;{\mu}g{\cdot}g^{-1}$), Yeongyang ($237.2\;{\mu}g{\cdot}g^{-1}$) in decreasing order. The concentration of phenolic compounds of common millets was the highest in the Norangchalgijang ($337.0\;{\mu}g{\cdot}g^{-1}$), Hwanggumgijang ($250.0\;{\mu}g{\cdot}g^{-1}$) was also relatively higher than others. The results of this study will provide basic information for breeding sorghums, foxtail millets and common millets with higher phenolic compound concentrations.

A Study on the Classification of Forest by Landsat TM Data (Landsat TM 자료를 이용한 임종구분에 관한 연구)

  • 최승필;홍성태;박재훈
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.11 no.1
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    • pp.55-60
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    • 1993
  • Forest occupied a part of natural ecosystem carries out a role of purifying air, preserving water resource, prevention of the breeding and extermination, recreation areas and etc that preserve and for me one's living environment. In this study, the classification for management of this forest is performed with Landsat TM Image. The classes are decided needle-leaf trees, broad-leaf trees, farming land and grass land, and water. When the TM digital images are classified on computer, water is represented in 7∼13 D.N. of 4th band. But the others is appeared similar mostly specific values so that must be done image processing. When the images compounded 2ed band and 3ed band are processed with ratio of enhancement. Needle-leaf treas is represented in l18∼136 D.N. of 1st band, broad-leaf trees in 72∼91 D.N. of 3ed band, farm land and glass land in 96∼120 of 3ed band. Forest Information is classified with M.L.C, an image classification method. The errors of needle-leaf trees, broad-leaf trees, farm land and grass land, and water are appeared each -7.43, +1.89,+7.58 and -2.04 as compared the digital image with investigation on the scene. Finally, these results are useful for classification of forest vegetation with Landsat TM Image.

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Evaluation of the Degrees of Genetic Connectedness Among Duroc Breed Herds (국내 두록종 농장간 유전적 연결성 추정)

  • Cho, Chungil;Choi, Jaekwan;Park, Byoungho;Kim, Sidong;Kwon, Ohsub;Choi, Youlim;Choy, Yunho
    • Journal of Animal Science and Technology
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    • v.54 no.5
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    • pp.337-340
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    • 2012
  • The genetic connectedness between herds is an essential requirement to make robust across-herd estimation of the breeding values of the animals. In this study, genetic connectedness between herds was evaluated by a connectedness rating method. A total of 24,971 records of days to 90 kg (D90KG) of the pigs on performance testing programs collected from six herds (labeled from 'A' to 'F') of Duroc breed along with pedigree information comprising 456,697 families were used. Results showed that a total of eight boars were used for semen exchange programs among participant farms. Herds 'A' through 'E' were found strongly connected among them. But 'F' herd was genetically connected strongly only with 'A' herd. The highest average connectedness rating was 91.7% between 'A' herd and 'C' herd. The lowest average connectedness rating was 65.1% between 'D' and 'F'. The concept of a single genetic group comprising six Duroc herds studied is meaningful due to high connectedness rates among them. Therefore, with this high genetic ties between participant Duroc farms, the more accurate genetic evaluation would be possible.

Classification and Selection of the Breeding materials in the Silkworm, Bombyx mori, by Multivariate Analysis 1. Classification of the Silkworm Genetic Stocks by Principal Component Analysis and Cluster Analysis (다변량 해석법에 의한 누에 육종소재의 탐색 1. 주성분분석과 집락분석을 이용한 누에품종분류)

  • 정도섭;이인정
    • Journal of Sericultural and Entomological Science
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    • v.31 no.2
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    • pp.102-112
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    • 1989
  • Principal component analysis and cluster analysis were performed on the nine quantitative characters of the one hundred and forty eight silkworm genetic stocks. The six major quantitative characters such as cocoon yield, cocoon weight, cocoon shell weight, cocoon shell percentage, larval period of the 5th instar silkworm, and total larval period showed significantly positive correlation between them. The first three principal components extracted form the initial nine variables by principal component analysis accounted for about eighty percent of original information. The first and second principal components were characterized as factors related to silk productivity, and cocoon productivity, respectively. On the basis of multivariate analysis using city block distance determined from the first three principal components to measure the phenotypic diversity, the one hundred and forty eight silkworm genetic stocks could be clustered into seven varietal groups, and the phenotypic diversity between the varietal groups was partly related to their geographical origins. Among 7 varietal group, group II and IV revealed higher silk and cocoon productivity.

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Morphological Characteristics and Classification of 25 Selected Clones of Aralia elata Seem (두릅나무 25개 선발 클론의 형태적 특성과 유연관계)

  • Kim, Sea Hyn;Kim, Moon Sup;Han, Jingyu;Kim, Hyeusoo;Moon, Heung Kyu
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.36-43
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    • 2013
  • Aralia elata Seem. have a typical traditional significance among the wild herbs traditionally. Edible shoots of A. elata will augment consumer's interest due to its high value functional value, eco-friendly and pesticide-free produce. A. elata's root, fruit and bark are used as material of hypoglycemic agent and medicine for diabetes, kidney trouble, acute hepatitis, rheumation arthritis, stomach cancer and gastroenteric trouble. Flavonoid glycoside compound which is separated from A. elata's shoot shows high antioxidative activity. Also, root's identified active materials of antimicrobial was reported to be produced as food preservative and handy antimicrobial. Therefore, this research investigated quantitative morphological characteristics of leaves, spine and bud in naturally dominated and introducted A. elata in south Korea and then considered its principal compound analysis(PCA) and classification analysis(CA) among the 6 improved cultivars and 19 clones. PCA results showed that it show 76% accumulated explanation from four PC. The A. elata clones were classified into five groups; the first group of 15 clones including Yeongok, the second group of 5 clones including Yeoju, the third group of Bonghwa, Ulleung, the fourth group of Yongmunsa, Boseong and the fifth group of Singu. The object of this study will give us invaluable information about breeding by selection of A. elata in south Korea.

Effect of Seed Weight and Storage Method on Germination and Seedling Growth of Camellia japonica (동백나무의 발아 및 유묘 생장에 미치는 종자 무게 및 저장방법의 영향)

  • Kang, Hee-Kyoung;Choi, Su-Ji;Song, Hong-Seon
    • Korean Journal of Plant Resources
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    • v.33 no.1
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    • pp.33-39
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    • 2020
  • This study was carried out to provide basic information for mass breeding and cultivating the saplings of Camellia japonica. The germination rates did not show definite tendency according to the changes of seed weight at room temperature and 4℃ dry storage conditions. The germination rate of 4℃ wet sand storage was increased with seed weight, but showed very low germination rate for 120 days of storage. The germination rate was above 80% in 60 days, 90 days and 120 days, respectively, at 4℃ wet filter paper storage conditions. The average days taken for those germinations were about 30 days at room temperature and 4℃ dry storage conditions, but wet storage (sand, filter paper) condition took the shortly nearly 13 days for those germinations. Leaf number, leaf dry weight, stem diameter and root dry weight were higher than total average by more than 1.21 g for seed weight. Stem length was higher than the average by more than 1.01 g, plant dry weight was higher than the average by more than 0.81 g, and stem dry weight was higher than the average by more than 0.61 g. In the case of seed weight becoming heavier, seedling growth was good but T/R ratio tended to decrease. In order to increase the mass production of seedlings using Camellia japonica seeds, we need to specify the weight and size of those seeds. The 4℃ wet filter paper storage condition was evaluated as the most efficient method for the seed storage used for seeding.

Morphological Characteristics and Genetic Diversity Analysis of Cultivated Sancho (Zanthoxylum schinifolium) and Chopi (Zanthoxylum piperitum) in Korea (국내 재배지의 산초(Zanthoxylum schinifolium)와 초피(Zanthoxylum piperitum)의 형태학적 특성과 유전적 다양성)

  • Ryu, Jaihyunk;Choi, Hae-Sik;Lyu, Jae-il;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.555-563
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    • 2016
  • The morphological characteristics and genetic relationships among 32 germplasms of Zanthoxylum schinifolium and Zanthoxylum piperitum collected from two farms in Korea were investigated. The traits with the most variability were seed color, leaf size, and spine size. The intraspecific polymorphism of Z. schinifolium and Z. piperitum was 96.5% and 60.3%, respectively. The genetic diversity and Shannon’s information index values ranged from 0.11 to 0.33 and 0.19 to 0.50, with average values of 0.26 and 0.42, respectively. Two ISSR primers (UBC861 and UBC862) were able to distinguish the different species. The genetic similarity matrix (GSM) revealed variability among the accessions ranging from 0.116 to 0.816. The intraspecific GSM for Z. schinifolium and Z. piperitum was 0.177-0.780 and 0.250-0.816, respectively. The GSM findings indicate that Z. schinifolium and Z. piperitum accessions have high genetic diversity and possess germplasms qualifying as good genetic resources for cross breeding. The clustering analysis separated Z. schinifolium and Z. piperitum into independent groups, and all accessions could be classified into three categories. Z. Schinifolium var. nermis belonged to independent groups. Comparison of the clusters based on morphological analysis with those based on ISSR data resulted in an unclear pattern of division among the accessions. The study findings indicate that Z. schinifolium and Z. piperitum accessions have genetic diversity, and ISSR markers were useful for identifying Z. schinifolium and Z. piperitum.