• 생물환경조절학회지
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• 제15권2호
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• pp.149-155
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• 2006

풋고추(청양)의 육묘 온도를 주간온도는 같게 하고 야간온도를 달리하여 유묘의 화아분화와 생장 및 포장에서의 생장을 조사하였다. 육묘기간 중의 야간 온도가 높을수록 고추 묘의 엽수, 지상부 생체중 및 건물중이 증가하였고, 화아분화 및 첫 개화일은 단축되었다. 정식 후 초장, 경경, 분지장, 절간장은 저온구($28/11^{\circ}C$)에서 증가한 반면 고온구($28/21^{\circ}C$)에서 억제되었다. 측지수는 고온구에서 현저히 감소되었으나 온도 처리에 따라 주경에서 발달하는 1차분지의 수는 일정한 경향을 보이지 않았다. 고추 모종의 생장은 저온구에서 억제되었다가 하우스에 정식한 후부터 점차로 회복된 반면, 고온구는 이와 반대의 경향을 보였다.

• The Korean Journal of Ecology
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• 제25권4호
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• pp.241-245
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• 2002

During the breeding season of 1998, breeding ecology of the Great Reed-Warbler (Acrocephalus arundinaceus orientalis) was studied at Yangsoo-ri and Yongdam-ri of the Yangpyung-gun, Kyunggi province, Korea. Egg-weight (CV: 6.25) was more variable than either length or breadth, and breadth was the least variable of the measures. Significant variations in overall egg-weight occurred between clutches, and that more of the total variation in egg-weight and shape are due to inter-clutch variation as to intra-clutch variation when the data were pooled. The last egg tends to be larger than the remaining eggs in the clutch of the Great Reed-Warbler, suggesting the Great Reed-Warbler may adopt the brood-survival strategy. When method 3 was used, the most common incubation period is 12 days. In the Great Reed-Warbler, the length of the incubation period was related to clutch-size when method 1 (r=0.485, p<0.05) and method 2 (r=0.621, p<0.01) were employed, but not related to egg weight. The averagee number of days of hatching asynchrony was 2.5, raging 0.5~2.5. Asynchronous hatching was related to the clutch size (r=0.66, p<0.01). Hatching sequence was closely related to the laying sequence (r=0.93, p<0.001), suggesting Great Reed-Warblers incubate their eggs before clutch completion. The effect of egg weight on hatching asynchrony was found in Great Reed-Warblers (t-test, p<0.01).

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.127-133
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• 2001

With an objective of evolving quantitatively and qualitatively superior bivoltine breeds/hybrids of silkworm Bombyx mori L. for tropical conditions, breeding work was initiated in Central Sericultural Research and Training Institute, Mysore during 1992 by utilizing two Japanese hybrids namely BNl8$\times$BCS25 and Shunrei$\times$Shogetsu along with Indian evolved breed, KA. The breed CSR12 which is characterized with sex-limited larval marking and white oval cocoons was evolved from the Japanese hybrid BNl8 ${\times}$ BCS25 by crossing with KA, while the breed CSR6 which is characterized with normal marking (marked larvae) and white dumbbell cocoons was extracted from the Japanese commercial hybrid Shunrei${\times}$Shogetsu through continuous inbreeding coupled with selection. After fixation, these breeds along with other newly evolved breeds were subjected to hybrid study under optimum environmental conditions in the laboratory for expression of full potential of the genotypes. These hybrids were evaluated by Multiple Trait Evaluation Index (Mano et al., 1993). The hybrid CSR12${\times}$CSR6 was selected based multiple trait evaluation index value. The hybrid CSR12$\times$CSR6 recorded survival of 96.0%, shell weight of 50.0 cg, shell ratio of 24.3%, raw silk percentage of 19.6, filament length of 1,216 m, boil off loss of 22.4% and renditta of 5.1. On the other hand, the control hybrid (KA ${\times}$ NB4D2) has recorded survival of 90.6%, shell weight of 42.1 cg, shell ratio of 20.4%, raw silk percentage of 15.9, filament length of 999 m, boil off loss of 24.8% and renditta of 6.3. The hybrid CSR12$\times$CSR6 was authorized during 1997 by Central Silk Board, Government of India for commercial exploitation during favourable months based on national level race authorization test.

• 한국육종학회지
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• 제42권3호
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• pp.241-244
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• 2010

할미꽃 신품종 '연홍'은 P. davurica와 P. koreana를 종간교잡하여 2003년에 전라남도농업기술원에서 육성되었고 유전적으로 자가불화합성의 특성을 가지고 있다. '연홍'의 화색은 진분홍이고 약은 진노랑, 주두는 진분홍, 꽃잎은 6매이며 엽은 녹색이다. 한편 개화특성은 년 2회 개화성이다. 주당 화경은 26.5개, 화경장과 절화장은 각각 58.6 cm와 46.8 cm, 포의 폭은 11.5 cm, 엽장은 34.4 cm이다. 꽃과 포의 절화수명은 각각 8.4일과 34일로 절화용으로 이용이 가능하다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.32-32
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• 2022

Abaca (Musa textilis L. Nee) is a native Musa species from the Philippines known for its natural fiber. Abaca fiber a.k.a. Manila hemp extracted from its pseudostems is considered one of the strongest fibers in the world. This is used for commodities such as ropes, papers, and money bills. Abaca is vulnerable to pests and diseases such as the Abaca Bunchy Top Disease (ABTD) caused by Abaca Bunchy Top Virus (ABTV) and Banana Bunchy Top Virus (BBTV). Inosa, one of the varieties of abaca utilized in the Philippines, is highly susceptible to ABTD. In contrast, Pacol (Musa balbisiana L.), a close relative of abaca, is highly resistant to the same disease. Here, we report the sequencing and de novo genome assembly of both abaca var. Inosa and banana var. Pacol. A total of ~16 Gb and ~21 Gb raw reads for Inosa and Pacol, respectively, were generated using Pacbio Hifi sequencing method and assembled with Hifiasm. High-quality de novo assemblies of both Musa species with 99% recovered as per BUSCO analysis were obtained. The assembled Inosa genome has a total length of ~654 Mb and N50 of 7 Mb while Pacol has a total length of 527 Mb and N50 of 3 Mb which are close to their estimated genome size of ~638 Mb and ~503 Mb, respectively. The information that can be derived from the de novo assembled genomes would provide a solid foundation for further research in disease resistance and fiber quality improvement in abaca.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.48-48
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• 2020

Assessment of plant genetic resources is applicable when breeding improved plant caltivars. In Korea, early maturing wheat germplasm is anticipated for wheat-rice double-cropping. In this study, we assessed five agronomical traits including days of heading after sowing(HD), days of maturity after sowing(MD), culm length(CL), ear length(EL), and thousand grain weight(TW) of 500 wheat germplasms collected form 10 different countries (Mexico, the United States, Afghanistan, Ethiopia, India, Japan, China, Turkey, Pakistan and Portugal), and grown in Korea. Besides, the correlation between the agronomical traits was analyzed using XLSTAT softerware version 2019 (Addinsoft, NY, USA). The result showed wide-ranging maturity period. Among the entire population, 2 accessions (K256306/JPN/breeding line and K256328/JPN/breeding line) that matured early were identified. Furthermore, HD showed strong correlation with MD (r=0.684) and CL (r=0.610), and weak correlation with the rest two agronomical traits (EL and TW). Overall, the results of our study provides wide spectrum of prospects, and could be applicable to breed new wheat varieties with early maturity.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.942-948
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• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

• 한국작물학회지
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• 제63권2호
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• pp.158-163
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• 2018

Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.

• 한국패류학회지
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• 제29권3호
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• pp.233-238
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• 2013

본 연구는 전복 먹이종류에 따른 성장 효율성 검토를 위하여 같은 어미에서 생산된 같은 크기의 개체를 사용하여, 동일한 사육환경 내에서 천연먹이인 미역 (UP), 다시마 (LJ) 및 시판용 전복 배합사료 (FD) 를 공급하였다. 그 결과, 각장형질의 성장은 미역 (UP) 및 다시마 (LJ) 를 공급한 실험구가 배합사료 (FD) 실험구 보다 유의적으로 빠른 것으로 나타났다. 반면, 증체율은 해조류를 공급한 실험구 (UP 및 LJ) 보다 배합사료 (FD) 실험구가 높게 나타났다. 이러한 결과는 먹이의 종류에 따라 패각의 성장과 근육부위의 성장이 각각 다르게 나타날 수 있다고 생각된다.

• Animal Bioscience
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• 제35권9호
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• pp.1303-1313
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• 2022

Objective: The current study aimed to perform whole-genome resequencing of Chinese indigenous Mongolian sheep breeds including Ujimqin, Sunit, and Wu Ranke sheep breeds (UJMQ, SNT, WRK) and deeply analyze genetic variation, population structure, domestication, and selection for domestication traits among these Mongolian sheep breeds. Methods: Blood samples were collected from a total of 60 individuals comprising 20 WRK, 20 UJMQ, and 20 SNT. For genome sequencing, about 1.5 ㎍ of genomic DNA was used for library construction with an insert size of about 350 bp. Pair-end sequencing were performed on Illumina NovaSeq platform, with the read length of 150 bp at each end. We then investigated the domestication and signatures of selection in these sheep breeds. Results: According to the population and demographic analyses, WRK and SNT populations were very similar, which were different from UJMQ populations. Genome wide association study identified 468 and 779 significant loci from SNT vs UJMQ, and UJMQ vs WRK, respectively. However, only 3 loci were identified from SNT vs WRK. Genomic comparison and selective sweep analysis among these sheep breeds suggested that genes associated with regulation of secretion, metabolic pathways including estrogen metabolism and amino acid metabolism, and neuron development have undergone strong selection during domestication. Conclusion: Our findings will facilitate the understanding of Chinese indigenous Mongolian sheep breeds domestication and selection for complex traits and provide a valuable genomic resource for future studies of sheep and other domestic animal breeding.