• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 연합학술대회
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• pp.289.1-289.1
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• 2003

• 농약과학회지
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• 제17권4호
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• pp.293-301
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• 2013

본 실험은 작물의 농약잔류성 포장시험 결과를 다른 소면적 작물로 외삽 할 수 있는지 알아보기 위하여 온실에서 재배되는 엽채소류를 대상으로 경엽살포 후 작물간 농약잔류 경향을 비교하였다. 배추속에 속하는 겨자채(Brassica juncea L.), 케일(Brassica oleracea L.), 다청채(a kind of pak-choi (Brassica rapa subsp. chinensis L.)), 잎 브로콜리(Brassica oleracea var alboglabra) 4종과 배추속이 아닌 들깻잎(Perilla frutescens Britt.), 상추(Lactuca sativa L.), 적근대(Beta vulgaris L. subsp. vulgaris) 및 적치커리(Cichorium intybus L. var. foliosum Hegi) 4종을 대상으로 하였다. 이들은 온실이나 노지에서 연중 재배되며 일정한 수확기 동안 연속적으로 잎을 따서 수확하는 작물들로서 시금치처럼 포기 형태로 일시에 수확하는 작물들과 구별된다. 무게 대비 엽면적 비율이 58 $cm^2/g$으로 가장 높은 들깻잎에서 imidacloprid 등 7종 농약의 잔류량이 살포 당일부터 5일 후 까지 가장 높았다. 따라서 식품의 일일섭취량 통계에 따른 섭취량이 일정 수준을 차지하면서 시험대상 8작물중 농약 잔류량이 상대적으로 높은 들깻잎을 농약잔류성시험의 대표작물로 선정하여 외삽 적용을 위한 작물 그룹을 제안한다.

• 생명과학회지
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• 제14권4호
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• pp.567-572
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• 2004

노화억제, 항균, cholesterol 저하작용 등 다양한 생리활성을 가지고, 기관지염, 천식 등 호흡기 질환에도 옛부터 효과가 있는 것으로 알려져 있으며 식품으로 애용되고 있는 적채를 metanol (BOM)로 먼저 추출하고 이를 hexane 분획물(BOMH), ethyl ether 분획물(BOMEE), ethyl acetate 분획물(BOMEA), butanol 분획물(BOMB) 및 물 분획물(BOMA) 등 다섯가지의 각 용매별로 분획하여 적채의 항균, 암세포 증식 억제 및 QR유도 효과를 연구하였다. 먼저 적채의 각 분획물을 Streptococcus mutans, Bacillus subtilis, Escherichia coli. Pseudomonas aeruginosa 및 Aspergil-lus oryzae의 5가지 균주에 첨가시료농도를 증가시키면서 첨가하였다. 즉, 각 분획 별 시료를 500, 1000, 1500 및 2000 $\mu{g}$/ml의 농도로 사용균주에 각각 처리하였을때 BOMEA에서 비교적 높은 항균 활성 효과를 나타내었고, 그 다음으로는 BOMEE이었다. 적채의 암세포 증식억제 효과(cytotoxicity)를 MTT assay 로 실험한 결과, 3종의 암세포주 HepG2, HeLa 및 MCF-7은 모두 BOMEE에서 높은 암세포 증식억제 효과를 보였으며, HepG2 세포주를 이용한 암예방 QR 유도 활성은 다른 분획층에 비해 비극성 용매층인 BOMH과 BOMEE에서 유의적으로 QR유도 활성을 증가시키는 것으로 나타났다. 이 결과를 기초로 독특한 보라색을 띄며 식탁에서 널리 애용되고 있는 자주색 대표식품인 적채의 항균 및 암세포 성장저지 및 QR 유도 효과를 일으키는 생리활성 물질의 존재와 기전 규명에 유익한 자료가 될 것으로 사료된다.

• 한국자원식물학회지
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• 제28권3호
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• pp.312-320
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• 2015

Total phenol, flavonoid and antioxidant components of cabbage leaf samples derived from different cultivar were determined. Total phenol compound content showed the highest amount in methanol extracts from ‘YR Howol’ cultivar (11.72 ㎎/g), followed by ‘Harutame’ (10.66㎎/g), ‘Winstar’ (10.34 ㎎/g) and YR Hero (10.20 ㎎/g). The highest amount of total flavonoid content was observed from the methanol extracts of Harutame (5.39 ㎎/g), followed by Winstar (4.28 ㎎/g), Wialhowol (4.10 ㎎/g). The SOD enzyme activity showed a high activity of ‘YR Hogeo’ cultivar, and the cultivar of ‘YR Howol’ cultivar showed the lowest activity of SOD. The activity of CAT and APX showed higher values ‘Ogane’ and ‘YR Hogeol’ cultivars than the other cultivars. The POD activities showed relatively high values ‘Ogane’ and ‘YR Howol’ cultivars compared with other cultivars. The free radical (DPPH) scavenging activity showed lower IC₅₀ values of ‘Harutame’ (15.71) and ‘YR Howol’ cultivar (16.88), however methanol extract of ‘YR Hero’ cultivar (22.49) being the highest. The extracts of all cabbage cultivars in the reaction solution of pH 1.2 could be decomposed nitrite more than 50%. Especially, the cultivar ‘YR Hogeol’ and ‘Ogane’ showed a relatively high nitrite scavenging activity for each 60.13% and 57.20% respectively. The IC₅₀ values of antioxidant activity determined by ABTS were lower in ‘Harutame’ (17.04) and ‘YR Howol’ cultivar (17.97), and its results observed similar with values obtained from the same extracts by DPPH method. The result of this study suggests that the methanol extract of Brassica oleracea L. contains the high amount of phenolic and higher radical scavenging activities.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.189-203
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• 2016

Brassica oleraceae var capitata is a member of the Brassicaceae family and is widely used as an horticultural crop. In the present study, transcriptome analysis of B. oleraceae L. var capitata was done for the first time using eight-week old seedlings treated with $50{\mu}m$ MeJA, versus mock-treated samples. The complete transcripts for both samples were obtained using the GS-FLX sequencer. Overall, we obtained 275,570 and 266,457 reads from seedlings treated with or without $50{\mu}m$ MeJA, respectively. All the obtained reads were annotated using biological databases and functionally classified using gene ontology (GO), the Kyoto Encyclopedia of Genes and Genomics (KEGG). By using GO analyses, putative transcripts were examined in terms of biotic and abiotic stresses, cellular component organization, biogenesis, and secondary metabolic processes. The KEGG pathways for most of the transcripts were involved in carbohydrate metabolism, energy metabolism, and secondary metabolite synthesis. In order to double the sequenced data, we randomly chose two putative genes involved in terpene biosynthetic pathways and studied their transcript patterns under MeJA treatment. This study will provide us a platform to further characterize the genes in B. oleracea var capitata.

• Food Science and Biotechnology
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• 제14권6호
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• pp.836-840
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• 2005

Extraction characteristics of Bonus species of Brassica oleracea var. capita and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 44.07% was obtained at ratio of solvent to sample of 27.94 mL/g, ethanol concentration of 24.35%, and extraction temperature of $55.21^{\circ}C$. At ratio of solvent to sample, ethanol concentration, and extraction temperature of 21.11 mL/g, 58.53%, and $68.83^{\circ}C$, respectively, maximum electron-donating ability was 48.44%. Maximum inhibitory effect on tyrosinase was 68.94% at ratio of solvent to sample, ethanol concentration, and extraction temperature of 24.08 mL/g, 10.49%, and $78.71^{\circ}C$, respectively. Superoxide dismutase (SOD) showed maximum pseudo-activity of 24.78% at ratio of solvent to sample of 22.66 mL/g, ethanol concentration of 45.69%, and extraction temperature of $93.81^{\circ}C$. Based on superimposition of four-dimensional RSM with respect to extraction yield, electron-donating ability, and pseudo-activity of SOD, optimum ranges of extraction conditions were ratio of solvent to sample of 20-30 mL/g, ethanol concentration of 35-65%, and extraction temperature of $50-80^{\circ}C$.

• International journal of advanced smart convergence
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• 제7권3호
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• pp.146-154
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• 2018

In this study we investigated antioxidant and anti-inflammatory activities of Malus Domestica (apple), Pyrus Communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage) and Raphanus sativus L. (radish), that were obtained from local market. As these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties especially the antioxidant and anti-inflammatory. The samples were processed by an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assay, processed samples showed better antioxidant activity compared to raw samples, and processed pear sample had the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. The mRNA expressions of pro-inflammatory mediators and cytokines (iNOS, COX-2, $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6) were assessed by RT-PCR. Processed samples exhibited better inhibition of iNOS, compared to the raw forms. Processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples did not exhibit cytotoxicity against RAW 264.7 cells up to 1mg/ mL as shown in the cell viability assay. Taken together, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

• Bulletin of the Korean Chemical Society
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• 제28권2호
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• pp.246-250
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• 2007

The crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared as a rich source of peroxidase and its enzymatic and specific activities in the presence of cross-linked polyvinylpyrrolidone as a stabilizer were determined. This extract was used to catalyze the oxidation of thiamine in the presence of hydrogen peroxide to thiochrome at pH 8.0. Thiochrome shows strong fluorescence at 436 nm with excitation at 370 nm. Based on the obtained results, a sensitive and simple spectrofluorimetric method was developed for the determination of thiamine. In the optimum conditions, the calibration graph was linear from 2 × 10-7 to 1 × 10-4 mol L-1, with a detection limit of 6.2 × 10-8 mol L-1. The relative standard deviation (RSD) was 1.2% for 5 × 10-6 mol L-1 thiamine. The method was successfully applied to the determination of thiamine in vitamin B1 and vitamin B complex tablets and vitamin B complex syrup.

• 한국식품영양학회지
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• 제22권2호
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• pp.229-237
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• 2009

Physical, textural and sensory properties of Seolgiddeok prepared with different amounts of broccoli(Brassica oleracea var. italica Plen.) powder were investigated during 3 days of storage. Moisture content decreased gradually during storage and was less in broccoli powder-amended samples. The color L value decreased significantly with increasing broccoli powder, whereas both redness and yellowness increased. Texture analyses revealed that hardness, chewiness, gumminess, adhesiveness and fracturability of Seolgiddeok tended to decrease in proportion to the amount of broccoli powder in the formula. Seolgiddeok gelatinization was investigated using amylographing. Break down and setback were low in broccoli powder Seolgiddeok. Sensory evaluations revealed that, Seolgiddeok prepared with broccoli powder was superior in flavor, chewiness, softness to unamended samples. Seolgiddeok prepared with 3% broccoli powder showed the highest overall acceptability score. Use of broccoli powder in Seolgiddeok preparation improves sensory characteristics and delays retrogradation.

• Journal of Plant Biology
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• 제38권2호
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• pp.137-141
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• 1995

We isolated a cDNA clone, BLSC1, encoding 5' exon of ATP synthase CF0 subunit I from broccoli. BLSC1 is 285 nucleotides long which consists of a 5' noncoding region of 34 nucleotides, a 5' exon of 145 nucleotides and an intron of 106 nucleotides. The 5' exon codes for 48 amino acids which reveals mostly hydrophobic. The amino acid sequence deduced from BLSC1 shares 83%, 83% and 91% identities with the genes coding for atpF from wheat, rice and spinach, respectively. Genomic Southern blot analysis for BLSC1 showed a typically strong signal for a gene located in the chloroplast genome. Northern blot analysis identified three major classes of transcripts showing strong positive signals in the leaves, but only trace amounts of the transcripts were identified in the other organs like stems, flowr buds and roots.