Browse > Article
http://dx.doi.org/10.7732/kjpr.2015.28.3.312

Antioxidant Activity of Several Cabbage (Brassica oleracea L.) Cultivars  

Yang, Sung-Ryeul (Department of Horticulture, Sunchon National University)
Songzhuzhao, (Department of Horticulture, Sunchon National University)
Boo, Hee-Ock (Department of Life Science, Chosun University)
Publication Information
Korean Journal of Plant Resources / v.28, no.3, 2015 , pp. 312-320 More about this Journal
Abstract
Total phenol, flavonoid and antioxidant components of cabbage leaf samples derived from different cultivar were determined. Total phenol compound content showed the highest amount in methanol extracts from ‘YR Howol’ cultivar (11.72 ㎎/g), followed by ‘Harutame’ (10.66㎎/g), ‘Winstar’ (10.34 ㎎/g) and YR Hero (10.20 ㎎/g). The highest amount of total flavonoid content was observed from the methanol extracts of Harutame (5.39 ㎎/g), followed by Winstar (4.28 ㎎/g), Wialhowol (4.10 ㎎/g). The SOD enzyme activity showed a high activity of ‘YR Hogeo’ cultivar, and the cultivar of ‘YR Howol’ cultivar showed the lowest activity of SOD. The activity of CAT and APX showed higher values ‘Ogane’ and ‘YR Hogeol’ cultivars than the other cultivars. The POD activities showed relatively high values ‘Ogane’ and ‘YR Howol’ cultivars compared with other cultivars. The free radical (DPPH) scavenging activity showed lower IC50 values of ‘Harutame’ (15.71) and ‘YR Howol’ cultivar (16.88), however methanol extract of ‘YR Hero’ cultivar (22.49) being the highest. The extracts of all cabbage cultivars in the reaction solution of pH 1.2 could be decomposed nitrite more than 50%. Especially, the cultivar ‘YR Hogeol’ and ‘Ogane’ showed a relatively high nitrite scavenging activity for each 60.13% and 57.20% respectively. The IC50 values of antioxidant activity determined by ABTS were lower in ‘Harutame’ (17.04) and ‘YR Howol’ cultivar (17.97), and its results observed similar with values obtained from the same extracts by DPPH method. The result of this study suggests that the methanol extract of Brassica oleracea L. contains the high amount of phenolic and higher radical scavenging activities.
Keywords
Brassica oleracea L.; Phenolic compounds; Antioxidant enzyme; DPPH; ABTS; Nitrite scavenging;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Wheeler, G.L., M.A. Jones and N. Smirnoff. 1998. The biosynthetic pathway of vitamin C in higher plants. Nature 393:363-369.   DOI
2 Zhu, N., M. Wang, G.J. Wei, J.K. Lin, C.S. Yang and C.T. Ho. 2001. Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechin gallate and pyrogallol with 2,2-diphenyl-1-picrylhydrazyl radical. Food Chemistry 73:345-349.   DOI
3 Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7:405-410.   DOI
4 Najami, N., J. Tibor, W. Barriah, G. Kayam, T. Moshe, M. Guy and M. Volokita. 2008. Ascorbate peroxidase gene family in tomato: its identification and characterization. Mol. Genet. Genom. 279:171-182.   DOI
5 Shahidi, F. and C.T. Ho. 2005. Phenolics in food and natural health products: an overview: In Shahidi, F. and C.T. Ho (eds.), Phenolic Compounds in Foods and Natural Health Products, ACS, Washington DC. (USA). pp. 1-8.
6 Shin, J.H., J.Y. Lee, J.C. Ju, S.J. Lee, H.S. Cho and N.J. Sung. 2005. Chemical properties and nitrite scavenging ability of citron (Citrus junos). J. Korean Soc. Food Sci. Nutr. 34:496-502.   DOI
7 Stratil, P., B. Klejdus and V. Kuban. 2006. Determination of total content of phenolic compounds and their antioxidant activity in vegetables―evaluation of spectrophotometric methods. J. Agri. Food Chem. 54:607-616.   DOI
8 Wojtaszek, P. 1997. Oxidative burst: an early plant response to pathogen infection. Biochem. J. 322:681-692.   DOI
9 Yokozawa, T., C. P. Chen, E. Dong, T. Tanaka, G.I. Nonaka and I. Nishioka. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochemical Phamacology 56:213-222.   DOI
10 Zhou, B.Y., Z.F. Guo and Z.L. Liu. 2005. Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress. Crop Sci. 45:599-605.   DOI
11 Huang, Y., A. Tan, Y. Shen and J. Lu. 1998. Scavenging effect of total flavonoids of Lycium barbarum L. on active oxygen radicals and inhibitory effects on heat output from L 1210 cells. Wei Sheng Yen Chiu. 27:109-111.
12 Gaspar, T., C. Penel, F.J. Castillo and H. Greppin. 1985. A two step control of basic and acid peroxidases and its significance for growth and development. Plant Physiol. 64:418-423.   DOI
13 Hollman, P.C., M.G. Hertog and M.B. Katan. 1996. Role of dietary flavonoids in protection against cancer and coronary heart disease. Biochem. Soc. Trans. 24(3):785-789.   DOI
14 Hong, T.G., Y.R. Lee, M.H. Yim and C.N. Hyun. 2004. Physiological functionality and nitrite scavenging ability of fermentation extracts from pine needles. Korean J. Food Preserv. 11:94-99.
15 Kusznierewicz, B., A. Bartoszek, L. Wolska, J. Drzewiecki, S. Gorinstein and J. Namiesnik. 2008. Partial characterization of white cabbage (Brassica oleracea var. capitata f. alba) from different regions by glucosinolates, bioactive compounds, total antioxidant activities and proteins. LWT. 41:1-9.   DOI   ScienceOn
16 Ismail, A., Z.M. Marjan and C.W. Foong. 2004. Total antioxidant activity and phenolic content in selected vegetables. Food Chem. 87:581-586.   DOI
17 Kang, H.M. and M.E. Saltveit. 2002. Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Physiol. Plant. 115:571-576.   DOI
18 Kato, F.T. and T.T. Puck. 1971. Mutagenesis by carcinogenic nitroso compounds. J. Cell Physiol. 78:139-144.   DOI
19 Lee, S.C., S.Y. Kim, S.M. Jeong and J.H. Park. 2006. Effect of far-infrared irradiation on catechins and nitrite scavenging activity of green tea. J. Agric. Food Chem. 54:399-403.   DOI
20 Choi, D.B., K.A. Cho, M.S. Na, H.S. Choi, Y.O. Kim, D.H. Lim, S.J. Cho and H. Cho. 2008. Effect of bamboo oil on antioxidative activity and nitrite scavenging activity. J. Ind. Eng. Chem. 14:765-770.   DOI
21 Asish, K.P. and B.D. Anath. 2005. Salt tolerance and salinity effects on plants. A review Ecotoxicol. Environ. Saf. 60:324-349.   DOI
22 Bonnet, M., O. Camares and P. Veisseire. 2000. Effects of zinc and influence of Acremonium lolii on growth parameters, chlorophyll a fluorescence and antioxidant enzyme activities of ryegrass (Lolium perenne L. cv Apollo). J. Exp. Bot. 51(346):945-953.   DOI
23 Ferreres, F., C. Sousa, V. Vrchovska, P. Valentao, J.A. Pereira, R.M. Seabra and P.B. Andrade. 2006. Chemical composition and antioxidant activity of tronchuda cabbage internal leaves. Eur. Food Res. Technol. 222:88-98.   DOI
24 Boo, H.O., J.H. Shin, J.S. Shin, E.S. Choung, M.A. Bang, K.M. Choi and W.S. Song. 2012. Assessment on antioxidant potential and enzyme activity of some economic resource plants. Korean J. Plant Res. 25(3):349-356.   DOI
25 Chun, O.K., N. Smith, A. Sakagawa and C.Y. Lee. 2004. Antioxidant properties of raw and processed cabbage. Int. J. Food Sci. Nutr. 55(3):191-199.   DOI
26 Dionisio-Sese, M.L. and S. Tobita. 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci. 135:1-9.   DOI
27 Foyer, C.H., P. Descourvières and K.J. Kunert. 1994. Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Env. 17:507-523.   DOI
28 Galati, G. and P.J. O’Brien. 2004. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopre-ventive and anticancer properties. Free Radic. Biol. Med. 37(3):287-303.   DOI
29 Ahmed, M.F., A.S. Rao, S.R. Ahemad and M. Ibrahim. 2012. Phytochemical studies and antioxidant activities of Brassica Oleracea L. var. Capitata. Int. J. Pharm. Sci. 4(3):374-378.
30 Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 5:373-99.
31 Asada, K. 1994. Production and action of active oxygen species in photosynthetic tissues: In Foyer, C.H. and P.M. Mullineaux (eds.), Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants. CRC Press, London, England. pp. 77-105.