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http://dx.doi.org/10.5352/JLS.2004.14.4.567

The Effects on Antimicrobial and Cytotoxicity of Brassica oleracea L. Fractions.  

박윤자 (신라대학교 식품영양학과, 마린-바이오 산업화 지원센타)
전광혜 (신라대학교 식품영양학과, 마린-바이오 산업화 지원센)
김선희 (부산대학교 의과대학 생화학교)
배송자 (신라대학교 식품영양학과, 마린-바이오 산업화 지원센타)
Publication Information
Journal of Life Science / v.14, no.4, 2004 , pp. 567-572 More about this Journal
Abstract
This study was carried out to investigate the affects on antimicrobial and cytotoxicity of red cabbage (Brassica oleracea L.,BO). In the paper disc test, antimicrobial activity of BO fractions was increased in proportion to its concentration. Among the various solvent fractions of methanol extract (BOM) of BO, the ethylacetate partition layer (BOMEA) showed the strongest antimicrobial activity We also determined the cytotoxicity and Quinone Reductase induced effect of BO extract and fractions on human cancer cells. The cytotoxicity of BO fractions on HepG2, HeLa and MCF-7 cells was evaluated by MTT assay. The BOMEE and BOMEA showed strong cytotoxic effects on all cancer cell lines we used. The quinone reductase induced effect of BO fractions on HepG2 cells, the hexane partition layer (BOMH) at a dose of 200 $\mu{g}$/ml was 2.88 times more effective compared to the control values of 1.0.
Keywords
antimicrobial; cytotoxicity; quinone reductase; Brassica oleracea L.(BO);
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