• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.285-292
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• 1997

This study was conducted to obtain the transgenic Brnssica napus plants with tobacco Apase gene using the binary vector system of Agrobacteriurn fumefociens. The results obtained were summarized as follows: A repressible acid phosphatase gene of Saccharon~yces cerevisiae, pho105 was used for screening of tobacco Apase cDNA. In order to identify Apase gene in tobacco genome, Southern blot analysis was pcrformed and the Apase gcnc may be present as a single copy, or at most two or three copies, in tobacco genome. To isolate the tobacco Apase gene, tobacco cDNA library was constructed using purifed mRNA from -Pi treated tobacco root and the plaque forming unit of the library was 2.8 x $10^5$ pfu/m${\ell}$, therefore the library might cover all expressed mRNAs. Using pho5 as a probe. tobacco Apase cDNA was cloned, and restriction mapping and Southern blot analysis of cDNA insert were revealed that the 3.6 kb cDNA contained tobacco acid phosphatase cDNA. Plasmid pGA695 -tcAPl was constructed by subcloning tobacco Apase cDNA into the Hind site of pGA695 with 35s promoter which can be expressed constitutively in plants. The Brassica napus cotyledonary petioles were cocultivated with the ,4 grobacteriunz and transferred to the selection medium. The transformed and regenerated plants were transplanted to soil medium. Southern blot analysis was done on the transformed plants, and it was confirmed that a foregin gene was stably integrated into the genonies of B. nnpus plants.

• Food Science and Biotechnology
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• v.17 no.5
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• pp.1097-1101
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• 2008

The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. 'Hanakkori' materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In 'Hanakkori', 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.233-239
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• 1983

In order to obtain basic date for the cultivation and the breeding of double zero varieties of rapeseed, spring nature grade were investigated. Spring nature grade could be classified to 8 classes by the method of material maturity. The double zero varieties, Ⅶ group of spring adaptability has been developed from the cross "Oro" and "Midas" classified as summer type. A significant negative correlation was found between the spring adaptability and seed yield. On the other hand, a highly significant negative correlation was found between maturation and spring adaptability, cold resistnce and spring adaptability, plant from and spring adaptability. Ⅵ and Ⅶ group close to summer type showed spring growing adaptability, and 0, I and II group were appeared as winter type, autumn growing adaptability with cold tolerance.with cold tolerance.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.315-322
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• 2013

BACKGROUND: This study was performed for the identification and quantification of glucosinolate (GSL) contents in seven varieties of rapeseed (Brassica napus L.) sprouts cultivated under dark and light conditions. METHODS AND RESULTS: Crude glucosinolates (GSLs) were desulfated by treating with aryl sulfatase and purified using diethylaminoethyl sepharose (DEAE) anion exchange column. Individual GSLs were quantified using high-performance liquid chromatography (HPLC) with electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Eleven GSLs including six aliphatic (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, and glucobrassicanapin), four indolyl (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) and one aromatic (gluconasturtiin) were identified based on the fragmentation patterns of MS spectrum. Aliphatic GSLs were noted as the predominant group with average 85.2% of the total contents. The most abundant GSLs were progoitrin which was ranged at $8.14-118.68{\mu}mol/g$ dry weight (DW). The highest total GSL amounts were documented in 'Hanra' ($146.02{\mu}mol/g$ DW) under light condition and 'Mokpo No. 68' ($86.67{\mu}mol/g$ DW) in dark condition, whereas the lowest was in 'Tamra' (30.13 and $14.50{\mu}mol/g$ DW) in both conditions. The sum of aliphatic GSLs attributed > 80% in all varieties, except 'Tamra' (67.7% and 64.9% in dark and light conditions, respectively) in the total GSL accumulation. Indolyl GSLs were ranged $2.41-15.73{\mu}mol/g$ DW, accounted 2.78-33.6% of the total GSLs in rapeseed varieties. CONCLUSION(S): These results provide valuable information regarding potential beneficial GSL contents individually. This study attempts to contribute to knowledge of the nutritional properties of the different varieties of rapeseed plants. These results may be useful for the evaluation of dietary information.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.317-323
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• 2012

For the establishment of an efficient embryogenesis from microspore culture in Brassica napus L. domestic cultivar 'Tammiyuchae', four different factors affecting microspore embryogenesis and plantlet regeneration were investigated. The highest embryogenesis rate was achieved when microspores at late uninucleate to early binucleate stage were isolated from flower buds with a length of 3.0~3.5 mm. On average, 388 embryos generated from 1 ml of microspores media. The highest number of embryos was obtained when microspores were subjected to $32.5^{\circ}C$ for 2 days. Embryogenesis of 'Tammiyuchae' was increased with increasing microspore culture density up to about $5{\times}10^4ea/mL$. Gradually higher culture density repressed embryogenesis of microspores. Regeneration rate of shoots from microspore-derived embryos was observed in MS solid medium supplemented with $0.5mg{\cdot}L^{-1}$ NAA and $1.0mg{\cdot}L^{-1}$ BA, and grew well in MS solid medium without plant growth regulators.

• BMB Reports
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• v.39 no.3
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• pp.297-303
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• 2006

As lipolytic enzymes, GDSL lipases play an important role in plant growth and development. In order to identify their functions and roles, the full-length cDNA of a GDSL lipase gene, designated BnLIP2, was isolated from Brassica napus L. BnLIP2 was 1,300 bp long, with 1,122 bp open reading frame (ORF) encoding 373 amino acid residues. Sequence analysis indicated that BnLIP2 belonged to GDSL family. Southern blot analysis indicated that BnLIP2 belonged to a small gene family in rapeseed genome. RT-PCR analysis revealed that BnLIP2 was a tissue-specific expressing gene during reproductive growth and strongly expressed during seed germination. BnLIP2 expression could not be detected until three days after germination, and it subsequently became stronger. The transcript of this gene was deficient in root of seedlings growing at different stages. When juvenile seedlings were treated by methyl jasmonate (MeJ), salicylic acid (SA) and naphthalene acetic acid (NAA), BnLIP2 expression could not be induced in root. Our study implicates that BnLIP2 probably plays an important role in rapeseed germination, morphogenesis, flowering, but independent of root growth and development.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.1
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• pp.15-24
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• 2011

The glutathione (GSH) concentration in leaves of different maturities and roots of forage rape (Brassica napus L.) supplied with four levels of external $SO_4^{2-}$ (0, 0.1, 1.0 and 2.0 mM) supply were measured. The relationships of GSH concentration with $SO_4^{2-}$ uptake, ATP sulfurylase (ATPS) and O-acteylserine (thiol) lyase (OASTL) activity were also assessed. The $SO_4^{2-}$ uptake increased in parallel with the external $SO_4^{2-}$ supply, while protein concentration was not significantly changed. The ATPS activity increased continuously with decreasing $SO_4^{2-}$ supply from 2.0 to 0 mM, while the OASTL activity decreased significantly only at S-deficient conditions (0 and 0.1 mM). The GSH concentration in the young leaves, middle leaves and roots continuously increased (except for between 1.0 and 2.0 mM in the middle leaves and roots) as the external S supply was increased, but no significant changes occurred in the old leaves. The increased endogenous GSH concentration, affected by the $SO_4^{2-}$ supply level, was significantly related with the decrease in ATPS activity in both leaves and roots, and the decrease in OASTL activity only in leaves..

• Korean Journal of Breeding Science
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• v.43 no.2
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• pp.145-153
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• 2011

To develop new varieties of rapeseed (Brassica napus L.), the seeds of three varieties 'Naehan', 'Tammi', and 'Halla' were treated with proton ion beams and gamma rays (0~2,000 Gy), and then the characteristics of the mutants induced were examined up to $M_5$ generation to select the lines with fixed useful traits. In $M_5$ generation, we had selected several lines that were highly fixed for some useful traits such as plant height, maturity and flower size; one line with both earlier maturity and shorter stem than wild type, one line with only earlier maturity, two lines with shorter stem, one line with large flower, and one line with chlorophyll mutation. Among them, NP600-1-1-198-2 (induced from variety 'Naehan' was treated with proton ion beams 600 Gy) was superior for its distinction from the original variety, uniformity and stability. The unique characteristics of NP600-1-1-198-2 were dark green leaves, green stem, yellow flower, and black seed coat. Its flowering date was April 14, eight days earlier than its original variety, while seed maturity date was June 16 (five days earlier) and plant height 105 cm (shorter by 10 cm). NP600-1-1-198-2 has 52 silique per panicle, 6.2 cm silique length, 23 seeds per silique, 4.2 g per 1000 seeds and 45.9% oil content. The seed oil contained 67.8% of oleic acid, 16.7% of linoleic acid and 7.3% of linolenic acid but no erucic acid.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.80-86
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• 2021

The purpose of this study was to determine the optimal sowing date for the spring sowing cultivation of rapeseed (Brassica napus L.). To determine suitable rapeseed sowing dates for spring sowing cultivation, changes in growth, flowering characteristics, and seed production were investigated in Muan, South Korea between 2019 and 2020. 'Jungmo7001' is the suitable variety for spring sowing because of its early flowering characteristics, high seed yield, and the fact that it occupies the highest cultivation area in South Korea. When the yield of 'Jungmo7001' was investigated in 2019 and 2020, the highest yields recorded were 243 kg/10a in February 28, 2019, and 294 kg/10a in February 18, 2020. As a result, the optimal rapeseed seeding period is considered to be mid-to-late February in the southern region. There was no statistically significant difference in seed crude oil content. The content of oleic acid (C18:1) decreased, but that of linoleic acid (C18:2) and of linolenic acid (C18:3) increased significantly depending on the sowing date.

• Journal of Environmental Science International
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• v.7 no.2
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• pp.123-132
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• 1998

The experiment was performed to elucidate the effect of simulated acrid rain on growth of the seven crops(caucus carota vu sativa DC. , Fagopyrum esculenkm Moench, Brassica cmpespis subsp. napus var. pekinensis Makino , Raphuus satlws vu. hortensis for. acnMormis Makino, Brassica dbogjabra Bailey, Caphslcum mum L., and Perilla frutescens Britton). The pH levels of simulated acid rain ranged pH 3.1, 3.6, 4.1, 4.6, 5.1 and 5.6. The germination 10 each crop was influenced from stimulated acrid rain except buckwheat and kale. A general decrease of growth in crops was observed with Increasing pH concentrations. The pattern of soluble protein was observed a tendency to decrease from acidic pH eradlents. According to acidity, total DNA contents of each crop was showed a definite reduction. In conclusion, plant growth was stimulated decreasln and the chanties of total Protein Patterns and BNA contents extracted from leaves trended with stimulated acrid rain was showed seriously.