• 동의신경정신과학회지
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• 제9궈1호
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• pp.45-57
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• 1998

The effect of HWANSODAN(HSD), on the level of brain antioxidants was examined in aged rat. The experimental groups were divided into three groups and treated as follows ; normal group(negative control), Vt.E administrated Group(HSD). The purified microsome from brain tissue, those were measired the amounts of oxidant materials like Malonfialdehyde(MDA) and H2O2, then activities of antioxidants enzymes like superoxide dismutase, catalase, NADPH-cytochrome P-450 reductase.The results were as follows;1. In TBA reaction to measure the amount of MDA, HSD group and Vt.E group did not showed signigicant decrease.2. In the formation of Hydrogen peroxide, HSD group and Vt.E group showed a little increase.3. The activity of Superoxide dismutase was increased significantly in HSD group and Vt.E group.4. In the activity of Catalase, Vt.E group was increased significantly and HSD group a little increased. 5. The activity of NADPH-cytochrome P-450 reductase in the HSD group and Vt.E group showed significantly increase.According to the above results, it is suggested that HWANSODAN(HSD) has some antioxidant effects on the tissue of brain.

• Parasites, Hosts and Diseases
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• 제44권1호
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• pp.21-26
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• 2006

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.

• 국제물리치료학회지
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• 제10권1호
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• pp.1687-1694
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• 2019

Background: Although cranial electrotherapy stimulation (CES) is reported to have positive effects on mental functions such as depression and sleep improvement, detailed studies regarding awakening, attention and concentration among brain waves reflecting brain activity are lacking. Objective: To examine the effects of cranial electrotherapy stimulation (CES) on various electroencephalograms (EEGs) reflecting brain activities. Design: Randomized controlled clinical trial (single blind) Methods: This study selected 30 healthy adult women in their 20s who volunteered for this experiment. A total of 30 subjects were randomly assigned to three groups (Sham group, 0.5 Hz CES group, and 100 Hz CES group). EEGs were measured before and after the single CES, and the results were compared and analyzed. Results: The relative theta, alpha, and gamma waves indicated no significant differences in the interaction effects between time and group. The relative fast alpha wave only showed significant differences in the interaction effects between time and group in P4. The relative slow beta wave only indicated statistically significant differences in the interaction effects between time and group in T3 and T4. The relative mid and fast beta waves showed statistically significant differences in the interaction effects between time and group in all areas. Conclusions: These results suggest that a CES of 0.5 Hz awakens consciousness and has a positive influence on brain activity, while a CES of 100 Hz has a positive influence on thinking activity accompanying mental load during concentrating on one subject.

• Journal of Nutrition and Health
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• 제21권1호
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• pp.1-11
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• 1988

어미쥐에 있어서 임신기와 수유 기간동안의 저단백 식이와 단백질 회복이 새끼쥐의 성장과 발달 즉, 세포 크기와 세포수, 뇌의 지질 함량과 신경 전달 물질에 미치는 영향을 검토하기 위하여 임신 2주째부터 정상식이(20% casein diet)와 저단백식이(11.7% casein diet)를 급여한 어미쥐에 의해 사육된 새끼쥐를 비교하였는데 그 결과는 다음과 같다. 1) 각군을 생후 21일째 희생시켰을때 체중 및 각 기관의 무게는 저단백식이군이 정상군에 비하여 유의하게 낮았으며(p<0.01), 임신기의 저단백식이군을 수유기에 정상식이를 급여하였을때 정상군 수준으로 회복되었다. 한편 체중에 대한 각 장기의 무게비를 나타내었을 때는 저단백식이군이 정상군에 비하여 높은 경향을 나타내었다. 2) 간의 DNA, RNA 함량 및 단백질 함량은 임신, 수유기의 저단백식이군이 정상군에 비하여 유의하게 낮았다(p<0.01). 한편, 임신기의 식이부족군(L-L군, L-L군)의 간의 체중에 대한 무게비가 임신기의 정상식이군(C-C군, C-L군)에 비해 증가하였으며 liver weight/DNA는 R-R군이 높았으며(p<0.01), C-C군, C-L군, L-C군은 비슷한 경향을 보였다. 3) 근육의 DNA, RNA 함량 역시 임신, 수유기의 저단백식이군이 정상군에 비하여 유의하게 낮았다(p<0.01). 한편 뇌의 체중에 대한 무게비와 brain weight/DNA는 저단백식이군이 정상군에 비하여 증가하였다. 5) 뇌의 총지질 함량은 정상 식이군보다 저단백 식이군이 낮았으며(p<0.01) 수유기 저단백식이에 의한 영향이 컸다. 한편 뇌의 콜레스테롤 함량은 임신기의 저단백식이군이 낮은 경향을 보였다(p<0.01). 한편 뇌의 acetylcholinesterase activity는 암컷의 경우에는 임신기 또는 수유기 또는 임신기와 수유기의 저단백식이에 의하여 영향을 받아 정상식이군(C-C군)에 비하여 낮았다(p<0.01). 본 실험의 결과는 어미쥐에 있어 임신, 수유기의 저단백식이는 새끼쥐의 성장 발달 즉 세포의 수, 세포크기, 단백질 함량, 뇌의 지질 함량 및 acetylcholinesterase activity에 영향을 미침을 나타낸다.

• 한국식품위생안전성학회지
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• 제10권4호
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• pp.279-282
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• 1995

Eight natural or semistynthesized monoterpenes were examined for their effects in rat brain monoamine oxidase(MAO) using benzylamine as substrate. Thujone and 3-carene were found to have the inhibition effects on rat brain MAQ activity, 38% and 95% inhibition at 103M respectively. The kinetic study on 3-carene, the most potent inhibitive type. But (+) pulegon and (-) isopulegon was found to activate MAO slightly.

• Bulletin of the Korean Chemical Society
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• 제32권6호
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• pp.2015-2020
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• 2011

A series of isoxazol-indolin-2-one was designed for GSK-3${\beta}$ inhibitors as novel anticancer agents based on their binding mode analysis in GSK-3${\beta}$ crystal structure. Total 21 compounds were synthesized and evaluated for their inhibitory activity against two tumor cell lines (DU145 and HT29). Most of the synthesized compounds were potent with above 80% inhibitory activity at 100 ${\mu}M$, and several compounds were examined for inhibitory activity against GSK-3${\beta}$. Among them, 15(Z) ($R_1$=H, $R_2$=3-Cl-phenyl) was most active with 78% inhibition of tumor cell line (HT29) at 20 ${\mu}M$ and 72% inhibition of GSK-3${\beta}$ at 20 ${\mu}M$.

• 대한임상검사과학회지
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• 제36권1호
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• pp.64-68
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• 2004

뇌 기능의 비가역적 중단을 뇌사라 정의한다. 뇌사를 진단하기 위해 선행되어야 할 조건은 종양, 뇌졸중, 사고 등에 의한 뇌 기능의 소실된 원인이 확실하여야 하고, 자발호흡의 비가역적 중단과 약물이나 급성 대사성 장애가 없어야 하고, 저 체온 상태가 아니어야 하며, 심장 쇼크 상태가 아니어야 한다. 뇌간 반사 작용이 없어야 한다. 뇌사를 진단하기 위한 검사로는 뇌파검사와 유발전위 검사에서 뇌로부터 나오는 파형이 없어야 하고, 뇌혈류와 angiography에서 혈류의 흐름이 없어야 한다. 이 논문은 뇌사를 진단하는 신경과 의사와 뇌신경 검사를 시행하는 임상병리사에게 도움이 될 것이다.

• 대한한방내과학회지
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• 제18권2호
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• pp.94-111
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• 1997

The present experiment was designed to examine catecholamines, serotonine, amino acids, malondialdehyde and free radical scavenging activity, by administering Yukmijihwangtang extract of a variety of concentraction to senile brain. The results were summarized as followings: 1. Yukmijihwangtang significantly increased noradrenaline in the striatum, hypothalamus, midbrain and pons-medulla oblongota of the brain tissue of senile rats, and even though Yukmijihwangtang increased noradrenaline also in other brain tissue, there was no significance. 2. Yukmijihwangtang significantly increased dopamine in the striatum, hypothalamus and midbrain of the brain tissue of senile rats, and even though Yukmijihwangtang increased dopamine also in other brain tissue, there was no significance. 3. Yukmijihwangtang significantly increased serotonine in the pons- medulla oblongata and cerebellum of the brain tissue of senile rats, and even though Yukmijihwangtang increased serotonine also in the other brain tissue except hypothalamus and midbrain, there was no significance. 4. Yukmijihwangtang significantly increased amino acid in the brain tissue of senile rats. 5. Yukmijihwangtang significantly decresed malondialdehyde and free radical in the brain tissue of senile rats. According to the above results, Yukmijihwangtang is assumed to improve brain function by reacting on biochemical of the senile brain, and that Yukmijihwangtang can be used to treat regressive brain disease carrying symptoms of psychoactive disorders.

• 감성과학
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• 제19권4호
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• pp.119-126
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• 2016

본 연구는 구강 호흡이 뇌기능에 미치는 영향을 뇌전도(EEG : electroencephalogram)를 통해 관찰하고자 한다. 신체가 건강한 12명의 피험자(남성: 6명, 여성: 6명, 나이: 21~27, 비흡연자)는 뇌파를 측정하기 위해 두피에 전극을 부착한 상태로 휴지기 상태에서 비강(Rest_N) 및 구강 호흡(Rest_M)을 수행하였고, 영어 대본을 사용한 청각언어자극이 주어지는 상황에서 비강(Eng_N) 및 구강 호흡(Eng_M)을 수행하였다. 각각의 뇌파는 뇌의 기능별로 크게 4 구역(R1~R4)으로 나뉘어 FFT (Fast Fourier Transform)을 통해 각각의 채널별(e.g., Pf1 and Pf2) 및 주파수 대역별(${\alpha}$, ${\beta}$, ${\gamma}$, ${\theta}$)로 절대 파워(Absolute Power) 비율을 살펴보았다. 도출된 결과에서는 Rest_N과 Rest_M 상태의 뇌파는 서로 유의미한 차이를 보이지 않았다. 비강 호흡 수행 중 청각언어자극이 주어졌을 때(Rest_N/Eng_N)의 뇌파를 비교했을 경우, 뇌파의 활동이 휴지기 상태의 뇌파 활동보다 통계적으로 유의미하게 높은 것으로 나타났다. 하지만 같은 조건상에서 구강호흡을 했을 때(Rest_M/Eng_M)는 비강 호흡을 실시했을 때와 달리 대부분의 뇌 구역과 주파수 대역에서 유의미한 차이가 나타나지 않았다. 동일한 조건의 자극에도 불구하고 구강 호흡을 하는 경우는 뇌기능의 변화가 비강 호흡과 다른 결과를 나타내었다.

• Toxicological Research
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• 제11권1호
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• pp.43-49
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• 1995

The effects of metallothionein and cadmium ion on the hepatic aldehyde oxidase activity and brain lipid peroxidation were tested in vitro. The content of brain lipid peroxide at the condition of normal or $300{\mu}M$ Fe(II)-induced was remarkably reduced by the addition of metallothionein in the incubation mixture. The induced content of lipid peroxide by cadmium $(30{\mu}g/ml)$ ion was reduced by metallothionein $(100{\mu}g/ml)$. The activity of aldehyde oxidase was not affected by metallothionein, but cadmium ion $(8.38{\mu}g/ml)$ increased the activity of aldehyde oxidase about 80% compared to the control. The cadmium-induced activity of aldehyde oxidase was restored to the control level by metallothionein or penicillamine.