Parasites, Hosts and Diseases

  • 제44권1호
  • /
  • Pages.21-26
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  • 2006
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지

In vivo determination of the gap2 gene promoter activity in Giardia lamblia

  • YANG Hye-Won (Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project, Yonsei University College of Medicine) ;
  • KIM Juri (Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project, Yonsei University College of Medicine) ;
  • YONG Tai-Soon (Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project, Yonsei University College of Medicine) ;
  • PARK Soon-Jung (Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project, Yonsei University College of Medicine)
  • 발행 : 2006.03.01
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초록

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.

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참고문헌

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