• Journal of Veterinary Clinics
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• v.20 no.3
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• pp.345-350
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• 2003

We were preferred bovine cortical bone to the others in xenobonegrafts for human and small animals, because those were not limited to supply and have sufficient size for bone transplantation. The strength (ST) and stiffness (SF) of cortical bone in bone grafts were very important. The strength and stiffness of cortical bone were much difference according to position of long bone in bovine limbs because which were biomechanical different to bear body weight. Therefore, we determinated by three bending point test methods the strength and stiffness of cortical bone which were collected in diaphysis of humerus, radius, femur and tibia of bovine. In the results, the strengths and stiffness among these were highest in radius by ST: 253.84$\pm$40.80 MPa, SF: 7.89$\pm$1.91 Gpa and lowest in humerus by ST: 185.69$\pm$28.54 MPa, SF: 6.21$\pm$1.22 Gpa.

• The Journal of the Acoustical Society of Korea
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• v.41 no.5
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• pp.557-563
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• 2022

The cortical bone thickness of the tibia is related to fracture risk and overall bone status. The present study aims to investigate the feasibility of two different ultrasonic methods for measuring the cortical bone thickness in bovine tibia in vitro. In the reflection technique, the tibial cortical thickness was determined from ultrasonic reflections from the periosteum and the endosteum producing specific peaks in the signal envelope. In the axial transmission technique, the tibial cortical thickness was determined from ultrasonic guided wave velocities measured along the axial direction of the tibia. The cortical bone thickness determined by using the reflection technique correlated significantly with that measured by using a caliper, with a Pearson's correlation coefficient of r = 0.97 (p < 0.0001). In contrast, the correlation coefficients for the axial transmission technique were r = 0.92 (p < 0.0001) for the first arriving signal method and r = 0.89 (p < 0.0001) for the slow guided wave method. Clinical feasibility should be demonstrated with an in vivo application to address the question whether the ultrasonic methods presented here could be useful as a screening tool for osteoporosis and potentially could be applied to other skeletal sites such as the femur and the radius.

• Korean Journal of Veterinary Research
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• v.48 no.1
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• pp.27-32
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• 2008

The present study was carried out to develop a sustained release implantable formula of bovine somatotropin (SRIF-BST) and to examine its sustained release effect. The SRIF-BST was produced by coating a solid pellet, which was comprised of BST and an excipient, made of a biodegradable polymer and poloxamer, which are capable of regulating the rate of BST release. The coated membrane of SRIFBST was observed with a field emission scanning electron microscope. The thickness of the coated membrane was approximately $1{\mu}m$, and the pore sizes of the coated membrane surface were below $10{\mu}m$. In dissolution test, the release duration of the SRIF-BST maintained for 10 days, whereas the release duration of the control BST formula maintained for 3 days. In weight gain assay and tibia test of hypophysectomized rats, the release duration of the SRIF-BST treated group was 12 days and the net weight gain was 53.16 g, also the tibia length and strength of the SRIF-BST treated group was increased 10.5% and 23.1% compared with those of the control group, respectively.

• Proceedings of the Acoustical Society of Korea Conference
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• spring
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• pp.373-376
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• 2002

Most previous studies using ultrasound for osteoporosis diagnosis have employed ultrasound in a frequency range of 0.2-1 MHz. In this study, acoustic properties of the 12 defatted bovine cancellous bone specimens were investigated in vitro. Speed of sound (SOS) and broadband ultrasonic attenuation (BUA) were measured using three matched pairs of transducers with the center frequencies of 1, 2.25, and 3.5 MHz, respectively, in order to cover a broad frequency range of 0.5-2 MHz. The relative orientation between ultrasonic beam and bone specimens was the mediolateral (ML) direction of the bovine tibia. SOS showed significant linear positive correlations with apparent density for all three pairs of transducers of 1 MHz, 2.25 MHz, and 3.5 MHz, respectively. BUA showed relatively weak correlations with apparent density for the pairs of transducers of 1 MHz and 2.25 MHz. Furthermore, in the measurement with the pair of 3.5 MHz transducers, BUA was independent of apparent density. SOS and BUA were only weakly correlated with each other. The linear combination of SOS and BUA showed significant correlations with apparent density. These results suggest that the frequency range up to 1.5 MHz may be also useful in the osteoporosis diagnosis.

• Archives of Craniofacial Surgery
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• v.12 no.1
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• pp.12-16
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• 2011

Purpose: This study examined the biomechanical stability of four different plating techniques in the experimental model of mandibular subcondyle fracture. Methods: Twenty standardized bovine tibia bone samples ($7{\times}1.5{\times}1.0cm$) were used for this study. Each of the four sets of tibia bone was cut to mimic a perpendicular subcondyle fracture in the center area. The osteotomized tibia bone was fixed using one of four different fixation groups (A,B,C,D). The fixation systems included single 2.0 mm 4 hole mini adaption plate (A), single 2.0 mm 4 hole dynamic compression miniplate (B), double fixation with 2.0 mm 4 hole mini adaption plate (C), double fixation with a 2.0 mm 4 hole mini adaption plate and 2.0 mm 4 hole dynamic compression miniplate (D). A bending force was applied to the experimental model using a pressure machine (858 table top system, $MTS^{(R)}$) until failure occurred. The load for permanent deformation, maximum load of failure were measured in the load displacement curve with the chart recorder. Results: Double fixation with a 2.0 mm 4 hole mini adaption plate and a 2.0 mm 4 hole dynamic compression miniplate (D) applied to the anterior and posterior regions of the subcondyle experimental model showed the highest load to failure. Conclusion: From this study, double fixation with an adaption plate and dynamic compression miniplate fixation technique produced the greatest biomechanical stability. This technique may be considered a useful means of fixation to reduce the postoperative internal maxillary fixation period and achieve early mobility of the jaw.

• Journal of Periodontal and Implant Science
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• v.30 no.3
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• pp.553-569
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• 2000

Many natural medicines have been studied for their capacity and effects of antibacterial, anti-inflammatory and regenerative potential in periodontal tissues. Safflower seed has been traditionally used as a drug for treatment of bone fracture in oriental medicine. The purpose of the present study was to compare the effects of safflower seed extract and bone substitute on bone formation and regeneration in artificial defects in mongrel dogs. The bony defects were made with round bur at mandible and tibia. Extracts of safflower seed and bovine bone were placed directly at each defect for experimental group, and the defect of control group was sutured without any other treatment. Experimental animals were sacrificed at 8 weeks. And then histopathologic reading and histomorphometric study was done. There was not significant differences between control and experimental groups in osteoclastic activity and infiltration of inflammatory cells. However, new capillary proliferation, fibrosis and new bone formation were prominent in safflower seed extract group. The mandibular defects of safflower seed extract group were healed with dense connective and bony tissues, and endochondral bone formation was observed in tibial defect of safflower seed extract group only. New bone area of safflower seed extract group was more significantly increased than that of control and that of bone substitute group. These results indicate that direct local application of safflower seed extracts on bony defects seems to reduces the early inflammatory response and to promotes the bone regeneration.

• Composites Research
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• v.25 no.1
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• pp.9-13
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• 2012

In this research, biomechanical characteristics of the bone tissue are experimentally investigated. By using specimens of the bovine bone, the mechanical properties are obtained through tension and shear tests. In experiments, non-homogeneous and anisotropic properties with respect to longitudinal and transversal directions are observed. Moreover, the viscoelastic behavior in which modulus and strength properties are dependent on strain rates is analyzed. It is expected that a numerical damage model of the bone be efficiently established based on the results.

• The Journal of the Acoustical Society of Korea
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• v.30 no.1
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• pp.56-62
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• 2011

In the present study, the phase velocities of guided ultrasonic waves such as the first arriving signal (FAS) and the slow guided wave (SGW) propagating along the long axis on the 12 tubular cortical bone samples in vitro were measured and their correlations with the cortical thickness were investigated. The phase velocities of the FAS and the SGW were measured by using the axial transmission method in air with a pair of unfocused ultrasonic transducers with a diameter of 12.7 mm and a center frequency of 200 kHz. The phase velocity of the FAS measured at 200 kHz exhibited a very high negative correlation with the cortical thickness and that of the SGW arriving after the FAS showed a high positive correlation with the cortical thickness. The simple and multiple linear regression models with the phase velocities of the FAS and the SGW as independent variables and the cortical thickness as a dependent variable revealed that the coefficient of determination of the multiple linear regression model was higher than those of the simple linear regression models. The phase velocities of the FAS and the SGW measured at 200 kHz on the 12 tubular cortical bone samples were, respectively, consistent with those of the S0 and the A0 Lamb modes calculated at 200 kHz on the cortical bone plate.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.38
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• pp.16.1-16.9
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• 2016

Background: The association of biomaterial combined with repair factor-like platelet-rich plasma (PRP) has prospective values. Bovine-derived xenograft has been identified as an osteoconductive and biocompatible grafting material that provides osseointegration ability. PRP has become a valuable adjunctive agent to promote healing in a lot of dental and oral surgery procedures. However, there are controversies with respect to the regenerative capacity of PRP and the real benefits of its use in bone grafts. The purpose of this study was to assess the influence of PRP combined with xenograft for the repair of peri-implant bone defects. Methods: Twelve rabbits were used in this study, and the experimental surgery with implant installation was performed simultaneously. Autologous PRP was prepared before the surgical procedure. An intrabony defect (7.0 mm in diameter and 3.0 mm deep) was created in the tibia of each rabbit; then, 24 titanium dental implants (3.0 mm in diameter and 8.5 mm long) were inserted into these osteotomy sites. Thus, a standardized gap (4.0 mm) was established between the surrounding bony walls and the implant surface. The gaps were treated with either xenograft alone (control group) or xenograft combined with PRP (experimental group). After healing for 1, 2, 3, 4, 5, and 6 weeks, the rabbits were sacrificed with an overdose of KCl solution. Two rabbits were killed at each time, and the samples including dental implants and surrounding bone were collected and processed for histological analysis. Results: More newly formed bone and a better bone healing process were observed in control group. The histomorphometric analysis revealed that the mean percentage of bone-to-implant contact in the control group was significantly higher than that of the experimental group (25.23 vs. 8.16 %; P < 0.05, independent-simple t test, analysis of variance [ANOVA]). Conclusions: The results indicate that in the addition of PRP to bovine-derived xenograft in the repair of bone defects around the implant, PRP may delay peri-implant bone healing.

• The Journal of Advanced Prosthodontics
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• v.6 no.5
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• pp.379-386
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• 2014

PURPOSE. These days, mesenchymal stem cells (MSCs) have received worldwide attention because of their potentiality in tissue engineering for implant dentistry. The purpose of this study was to evaluate various growth inducing factors in media for improvement of acquisition of bone marrow mesenchymal stem cells (BMMSCs) and colony forming unit-fibroblast (CFU-F). MATERIALS AND METHODS. The mouse BMMSCs were freshly obtained from female C3H mouse femur and tibia. The cells seeded at the density of $10^6$/dish in media supplemented with different density of fetal bovine serum (FBS), $1{\alpha}$, 25-dihydroxyvitamin (VD3) and recombinant human epidermal growth factor (rhEGF). After 14 days, CFU-F assay was conducted to analyze the cell attachment and proliferation, and moreover for VD3, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was additionally conducted. RESULTS. The cell proliferation was increased with the increase of FBS concentration (P<.05). The cell proliferation was highest at the density of 20 ng/mL rhEGF compared with 0 ng/mL and 200 ng/mL rhEGF (P<.05). For VD3, although the colony number was increased with the increase of its concentration, the difference was not statistically significant (P>.05). CONCLUTION. FBS played the main role in cell attachment and growth, and the growth factor like rhEGF played the additional effect. However, VD3 did not have much efficacy compare with the other two factors. Improvement of the conditions could be adopted to acquire more functional MSCs to apply into bony defect around implants easily.