• The Korean Journal of Malacology
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• v.17 no.1
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• pp.35-44
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• 2001

This research was conducted to develop seedling production techniques in flat oyster, Ostrea dense lamellosa. The cultivation of larvae, artificial spat and spats collection in natural conditions rate were examined. In the mass culture tank (3 tons), average growth rates of the D Shape larvae of initial shell length (153.4 ㎛) and shell height (153.4 ㎛) were 202.6% and 212.9% at 16 days and 227.1%, 241.8% at 20 days, respectively. Instantaneous death and survival rate of the larvae were 0.160 and 54.8% at 16 days and 0.057 and 43.2% at 20 days, respectively. Collection rate of flat oyster from bottom using various collectors with oyster shell, scallop shell, ark shell and PVC plates were 32.9%, 24.1%, 16.8% and 10.0%, respectively. and the greatest collecting rate was 131.9 individuals/shell (32.9%) in laid collectors on the bottom. The collecting rate of the oyster larvae were better in laid collectors on the bottom 83.8 individuals/collection than in the suspended string 54.2 individuals/collection. in all collecting substrates. Early spats settled on collectors were grew to 2.38 $\pm$ 0.97 ㎜ in shell length and 2.16 $\pm$ 0.86 ㎜ In shell height at 26 days. The spats were grew to 28.58 $\pm$ 2.39 ㎜ in shell length and 31.65 $\pm$ 2.03 ㎜ in shell height during the 4 month mid-term cultivation. In the period of cultivation, the mean number of spats attached to collectors were 10.3 individuals at oyster shells, 5.8 scallop shells, 4.0 ark shells and 1.5 PVC plates, respectively.

• Journal of Animal Science and Technology
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• v.54 no.2
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• pp.103-109
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• 2012

Present study was performed to investigate the effect of single and co-culture of adipocyte and muscle cell lines on cell differentiation. 3T3-L1 (adipocyte) and L6 (muscle) cell lines were single-cultured on the condition of 10% fetal bovine serum (FBS)/Dulbeco's modified eagle's medium (DMEM) for 48 h followed by culture within 5% FBS/DMEM as a growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without additives in single- or co-culture of the 3T3-L1 and the L6 cells to induce differentiation of both cell types. In co-culture system, the 3T3-L1 and the L6 cells were grown in separated places by being seeded on a $0.4{\mu}m$ insert membrane and on the bottom of 6 well plate, respectively. Cell differentiation was measured using morphological investigation and cytosolic analysis of glycerol-3-phosphate dehydrogenase (GPDH; for 3T3-L1) and creatine kinase (CK; for L6). Based on the GPDH results, the presence of L6 cells did not stimulate 3T3-L1 differentiation showing more differentiation of 3T3-L1 cells in the single-culture compared to the co-culture condition. In contrast, 3T3-L1 cells in the co-culture promoted differentiation of L6 cells. Enzymatic analysis supported this result showing that 3T3-L1 cells showed statistically (P<0.05) higher GPDH activity in the single-culture than the co-culture, whereas CK results of L6 cells were vice versa (P<0.05). Overall, present results may indicate that co-culture system is more reliable and precise technique compared to single-culture. Further studies on several co-culture trials including different media conditions, supplementation of differentiating substances, molecular biological analysis, etc. should be required to obtain practical and fundamental mass data.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.69-76
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• 2011

This study describes the effect of myo-inositol on sustained cell division and plant regeneration from cotyledon-derived protoplast of cabbage (Brassica oleracea var. capitata). Freshly isolated protoplasts were cultured in modified Murashige and Skoog (MS) medium removed ammonia ions and containing $0.4\;mg\;l^{-1}$ thiamine HCl, $100\;mg\;l^{-1}$ myo-inositol, $2\;mgl^{-1}$ 2,4-D, $0.5\;mgl^{-1}$ BA, $30\;gl^{-1}$ sucrose and several concentrations of myo-inositol (2, 4, 6, 8, 10% (w/v)) as an osmotic stabilizer. After 3 weeks of culture in the dark at $25^{\circ}C$, the plating efficiency of cabbage protoplasts reached to $22.5{\pm}2.9%$ when cultured in modified MS medium supplemented with $2\;mgl^{-1}$ 2,4-D, $0.5\;mgl^{-1}$ BA, $30\;gl^{-1}$ sucrose and 8% (w/v) of myo-inositol at a density of $2{\times}10^5$ protoplasts/ml. Rapidly growing cell colonies after 3 weeks of culture were transferred to the same culture medium removed osmoticum. To induce shoot regeneration from calluses, calluses with about 2 mm in diameter were transferred to the MS medium containing $2\;mgl^{-1}$ BA and $0.5\;mgl^{-1}$ NAA. After further three weeks of incubation onto the medium in the light, green shoots were formed on the surface of calluses at a frequency of 30%. Upon transfer to half-strength MS basal medium, roots were formed onto the bottom of regenerated shoots without auxin treatments. These regenerated plantlets were successfully acclimatized to soil transfer, grown to normal mature plants. The cabbage protoplast culture system established in this study could be applied for production of somatic hybrids or cybrids by asymmetric protoplast fusion and mass proliferation of elite somatic clones of cabbage.

• The Korean Journal of Malacology
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• v.31 no.2
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• pp.129-136
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• 2015

To introduce an oyster rack culture in tidal flat of Wando-gun, Korea, we investigated sanitary conditions from 2012 April to 2013 March in oyster growing waters and adjacent area in Wando. Average seawater temperature and salinity ranged $8.6-28.0^{\circ}C$ and 26.2-33.6 psu, respectively. The coliform group and fecal coliform of seawater ranged < 1.8-13.0 MPN/100 mL each. Only one of 60 samples was exceeded in the European criteria of fecal coliform. Influence of adjunct basin's pollution load on cultural waters was negligible, which half radius of dilution was not above 300 m. According to our observation, however, landing of oyster should be banned for at least 5 days after the heavy rainfall. Our findings meet the sanitary seawater criteria for domestic use and export of shellfish and classified as 'Class A' in European Community regulations for bivalve culture.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.225-230
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• 2010

This study was conducted to increase the income of cactus farm by selecting cheaper and better media than the peat moss using for Gymnocalycium mihanovichii 'Huhong'. We cultivated Gymnocalycium mihanovichii 'Huhong' on 10 kinds of media (peat moss, culture medium, coco peat, sphagnum moss, hydro ton, hydro cray, hydro bal, hugato, vermiculite, perlite) and analyzed media's physiochemical factors and growth, betacyanin. The results were as follows: In case of media's physical condition planting Gymnocalycium mihanovichii 'Huhong' after 90 days, an approximate value with peat moss is culture medium and coco peat. Also, coco peat has no change in chemical media. The rate of growth and development is high in Coco peat while overall culture medium and coco peat was seen lower growth. The level of betacyanin in subirrigation is higher than overhead irrigation. Meanwhile, hugato among 10 kinds of media has high value in both of overhead irrigation and bottom watering. Thus, culture medium and coco peat is proper for alternation of peat moss due to similar value with peat moss. And coco peat is favorable to media, growth condition, pigment.

• Journal of fish pathology
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• v.21 no.1
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• pp.35-43
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• 2008

Pathological symptoms and hematological parameters of red sea bream, Pagrus major and water temperature in the culture ground was investigated to clarlify the cause of winter moratility. Dead fish showed green liver and accumulation of ascites in the cavity. A few Bivagina tai were also found on the gill but either bacteria or virus were not. When hematological parameters from fish taken before/after the winter mortality were compared, blood glucose, serum AST and ALT, and total cholesterol, trigyceride and total protein were significantly decreased in the fish after the winter mortality. These results may explain that the nutritional level of fish was decreased because fish could not fed during winter season. According to the CORI monitoring system operating by KODC, NFRDI a long term water temperature from Dec. 25, 2005 to Feb. 24, 2006 (60 days) were exposed the low water temperature environments to the red sea bream. First of all, mass mortality began at Sarayngdo where low temperature below 8℃ continued for 42 days. The winter mortality did not occured in the depths of 9 m to 19.2 m where difference of water temperature in the surface and bottom was only within 0.1℃. But in the depth of 7.5 m to 11 m winter mortality occured where water temperature in the surface and bottom showed much variation ranged from 0.2℃ to 1.4℃. From these results, great difference of water temperature in the surface and bottom of the culturing area might results in winter mortality of red sea bream.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.468-473
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• 2005

The coastal regions of Yeosu, the South Sea of Korea, has occurred annually the red tide which is caused by potentially ichthyotoxic dinoflagellate C. polykrikoides, with a wide avenue for exchange with oceanic waters and freshwater runoff from Sumjin river. We attempted to examine the variability in response to vertical migration and concentration of extracted DNA/RNA of C. polykrikoides exposed to salinity-stratified waters. The experimental aquarium of the 60 liter was employed to culture C. polykrikoides. One aquarium was supplied with only sea water, the other was consisted of sea water and freshwater. Experiment was conducted for 5 days. In experimental column (mixture of freshwater and sea water), salinity was maintained to 20 at upper and approximately 30 at bottom during the period of this study. The fluctuation with related to dissolved oxygen and pH was similar pattern to both columns. Chlorophyll a was significantly higher value at upper than bottom. During 24h, chlorphyll a on experimental column was extremely high on the top as soon as lighting, compared with control. With elapsed time, the gap between experimental and control columns was a little. In darkness, chlorophyll a was not significantly different between upper and bottom, most cells appeared to randomly distribute on column regardless of water layer. Fluctuation with related to concentration of extracted DNA and RNA based on ratio of absorbance of 260 and 280 nm in experimental column was higher at final day or diel migration than control. These results implied that a large volume of freshwater could be associated with influence of concentration of DNA and RNA, in particular, rapid upward movement caused massive fish kills as soon as sunset.

• The Korean Journal of Malacology
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• v.16 no.1_2
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• pp.49-54
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• 2000

The growth of short neck calms, Tapes philippinarum was compared between two areas, Songdo in Kamak Bay and Sudo in Chinhae Bay from April 1999 to March 2000. The short neck clams in Kamak bay grew from 13.84${\pm}$0.04 mm to 35.43${\pm}$1.22 mm in shell length, while those in Chinhae bay grew from 13.84${\pm}$0.04 mm to 31.91 ${\pm}$1.72 mm in 12 months. Shell height, shell breadth and total weight of the short neck clams in Kamak bay were also greater than those from Chinhae bay. Bottom quality of Chinhae bay showed negative skewness, and that of Kamak bay was positive skewness of Chinhae bay seems to be caused by the effect of strong tidal current. This may indicate that Kamak bay is better area than Chinhae bay for the culture of the short neck clams.

• Journal of the Korean Society of Clothing and Textiles
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• v.39 no.5
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• pp.641-655
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• 2015

This study examined the ideal image and fashion of Korean women in the 1970s from a socio-cultural context. This study used information on the 1970s politics, economy, and culture provided by "Chosun Ilbo" and "Yosungjungang" as well as their presentation of the ideal image and fashion for Korean women in the 1970s. The ideal image and fashion of women were considered from the viewpoint of Hamilton's Meta-theory. The ideal image of women in the 1970s is divided into two aspects. The image from the traditional Confucian perspective was prevalent and restricted the lives of women to housekeeping chores. On the contrary, women have increasingly participated in society vis-$\grave{a}$-vis education and employment opportunities to present a progressive image of women. These aspects coexisted during the turmoil of social change. Progressive women had money to buy clothes because they were economically independent. These women embraced styles that included mini, midi, maxi, and bell-bottom pants. Further, pants were developed into different styles such as pant suits. T-shirts and blue jeans as casual wear were very popular among the youth. At the end of the 1970s, the tailored look and the big look (which copied men's clothing) were in fashion. Masculine styles such as wide shoulders with pads and neckties strengthened gender equality. Other fashions were dominated by feminine styles described as beautiful, sweet, and elegant that reflected Korean society's tendency to regard women as sex objects. Clothing that exposed the body highlights this sexual objectification aspect. Women wore miniskirts, hot pants, and bikinis because they wanted to enhance their sex appeal, propagating the view of women as sex objects. In conclusion, all aspects of society and culture were closely interrelated with a fashion style that reflected the values of those aspects.

• Journal of Soil and Groundwater Environment
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• v.20 no.6
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• pp.73-84
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• 2015

Nine plant species were selected through vegetation survey at three military shooting ranges at northern Gyeonggi Province. Plants were germinated in normal soil and three seedlings were transplanted to a bottom sealed pot containing sandy loam soils contaminated with either RDX (291 mg/kg) or TNT (207 mg/kg). Planted, blank (without plant), and control (without explosive compound) pots were grown in triplicate at a green house for 134 days. During cultivation, transplanted plants exhibited chlorosis and necrosis in flower and leaf by explosive toxicity and stress. Only three plants, Wild soybean, Amur silver grass, Reed canary grass, survived in TNT treated pot, while seven plant species except for field penny cress and jimson weed, thrived in RDX treated pot. Appreciable amount of TNT (61.6~241.2 mg/g-D.W.) was detected only in plant roots. Up to 763.3 mg/g-D.W. along with 4-amino-2,6-dinitrotoluene, an intermediate of TNT, accumulated in the root of wild soybean. In addition, azoxy compounds, abiotic intermediates of TNT, were detected in TNT treated soils. RDX absorbed average 1,839.95 mg/kg in shoot and 204.83 mg/kg in root. Most of TNT in plant was accumulated in underground part whereas RDX was localized in aerial part. Material balance calculation showed that more than 95% of the initial TNT was removed in the planted pots whereas only 60% was removed in the blank pot. The amount of RDX removed from soil was in the order of Amur Silver Grass (51%) > Chickweed (43%) > Evening primrose (38%). Based on the results of pot cultures, Amur silver grass and Reed canary grass are selected as tolerant remedial plants for explosive toxicity.