• Journal of Life Science
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• v.15 no.1 s.68
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• pp.100-105
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• 2005

The lactate dehydrogenase (EC 1.1.1.27, LDH) inhibitors were isolated from the LDH-free crude mitochondrial fraction of skeletal muscle in Syrian hamster (Mesocricetus auratus) and Korean native cattle (Bos taurus coreanae). The LDH inhibitor in skeletal muscle of M. auratus was successfully isolated by the treatment with 175 mM NaCl and ultrasonic. The LDH inhibitor in skeletal muscle of B. taurus coreanae was highly stable to heat and LDH fu isozyme was largely inhibited by the LDH inhibitor. The molecular weight of inhibitor was 22 kDa. Inhibitor played an important role in the binding of LDH with the mitochondria in tissues of skeletal muscle, kidney and liver except heart.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2016.10a
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• pp.257-257
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• 2016

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.689-694
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• 1999

This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.

• Parasites, Hosts and Diseases
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• v.56 no.2
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• pp.121-127
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• 2018

To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately $0.21-1.25{\mu}m$ in length and $0.05-0.07{\mu}m$ in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.

• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.245-250
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• 2005

This study was carried out to find out how space allowance affect the social behavior of Korean native cattle (Bos taurus coreanae) steers. Twelve Korean native cattle (Bos taurus coreanae) steers were used as subjects, each of which was 30-month-old and observation period was from June to July 2003. Five (T1) and seven (T2) steers were allotted to two pens of $5m{\times}10m$ in a building with slate roof and open sides respectively. They were fed at 09:00 h and 16:00 h, twice a day. The behaviors of steers were recorded from 06:00 h to 17:00 h, using two color CCD cameras (Samsung SDC-411, Korea), one B/W CCD cameras (Samsung SBC-340, Korea), one multiplexer (Samsung SDM-081, Korea) and a time lapse VCR (Samsung SRV-30, Korea). The behaviors of each steer were recorded every 2 min using an instantaneous point sampling method. While the mean percentage of time budget in WA of T1 was lower than that of T2 (p<0.05), the mean percentage of time budget in SF of T1 was higher than that of T2 (p<0.05). When it gets hot, steers in T1 rested from 10:00 h to 14:00 h when it gets cool, showing 40~80% of LD rate while steers in T2 rested from 12:00 h, when it very hot to 17:00 h, showing 20~50% of LD rate, which is relatively low. Steers in T1 were fed from 06:00 h to 08:00 h when it was cool and from 16:00 h to 18:00 h, showing 20~45% of EA rate while steers in T2 were fed from 08:00 h to 14:00 h when it was hot, showing 25~50% of EA rate. In conclusion, it turned out that the number of steers affected their social behavior, and T1 was better environment than T2 in terms of welfare.

• Journal of Veterinary Clinics
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• v.38 no.5
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• pp.254-259
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• 2021

Two cases of sudden death without clinical signs in a four-month-old female Hanwoo (Bos taurus coreanae) and sixteen-month-old female Holstein (Bos taurus taurus) were investigated. Severe pneumonia and septicemia were found on postmortem examination. Petechial and ecchymotic hemorrhages were present in the subcutaneous tissues of the neck region. The lungs remained expanded without collapsing, and the mediastinal lymph nodes were enlarged. A significant number of hemorrhagic bubbles were present in the tracheal lumen. On histopathology, the alveolar blood vessels were moderately congested, and inflammatory cells and multinucleated syncytial cells were found in the alveolar space. The pathogen isolated from the lesion was identified as Bibersteinia trehalosi using MALDI-TOF and 16S rRNA gene and sodA gene sequence analyses. To the best of our knowledge, this is the first report of B. trehalosi infection in cattle in Korea.

• Korean Journal of Veterinary Research
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• v.42 no.4
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• pp.437-442
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• 2002

The observations of the anatomical closure of the ductus venosus (DV) and vestige of DV were studied in 22 cattle, ranging from 210-day-old fetus to 3-years old Korean native cattle. Vinylite solution was injected into the hepatic, portal, umbilical veins and caudal vena cavae of 22 specimens for vinylite corrosion casts. The DV originated at the confluence of the umbilical and portal veins and emptied into the left hepatic vein or posterior vena cava. The DVs were persisted in a 210-day-old fetus, a 240-day-old fetus, and a 270-day-old fetus. Two newborns, two 2-year-old and two 3-year-old cattle had no rudimentum of DV (6 cases, 31.58%). However, vestiges of DV in varying sizes were observed in a 14-day-old, a 3O-day-old, two 180-day-old and nine adult cattle (13 cases, 68.42%). The lengths of vestiges of DV were about 4.97~99.66 mm. Therefore, the present study demonstrates that DV in cattle can be degenerated during the late period of a pregnancy.

• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.403-410
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• 2002

This study was performed to investigate the general prepartum behavioral ethogram of Bos taurus coreanae (Hanwoo cow). In this study, 4 pregnant cows were placed in a separate area. We recorded the behaviors of the cows using time lapse VCR for 48 hours and analyzed behaviors with the scan point sampling method. We observed maintenance behaviors, social behaviors and ingestion behaviors. During the observation period, the time budgets of behaviors in order of frequency were LD(lying down, 38.2%), ST(standing, 24.7%), EA(eating, 10.7%), WA(walking, 7.2%), LR(lying down rumination, 5.6%), SR(standing rumination, 3.3%), TW(tail wagging, 3.1%) and SG(self grooming, 1.8%). The time budgets of the other behaviors such as PG(pairwise grooming), FC(fly catching) were negligible (<1%).

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.12
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• pp.1974-1979
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• 2018

Objective: This study compared fatty acid compositions and antioxidant activities of high-preference cuts (loin, tenderloin, and rib) and low-preference cuts (brisket, topside, and shank) of Hanwoo (Bos taurus coreanae) cows to obtain potentially useful information for promoting the consumption of various low-preference cuts. Methods: Individual 500 g samples of fresh beef were collected from each of the six cuts from 10 Hanwoo cows (quality grade 1) and immediately freeze-dried. The dried samples were evaluated for fatty acid composition, free radical scavenging activities (hydroxyl, alkyl, and 2, 2'-diphenyl-1-picrylhydrazyl [DPPH] radical), and antioxidative enzyme activities (glutathione peroxidase [GPx], glutathione-S-transferase [GST], and superoxide dismutase [SOD]). Results: The percentages of total polyunsaturated fatty acids were significantly higher in low-preference cuts than in high-preference cuts (p<0.05). Hydroxyl, alkyl, and DPPH radical scavenging activities were significantly higher in low-preference cuts than in high-preference cuts (p<0.05). In addition, the activities of antioxidant enzymes, such as GPx, GST, and SOD, were significantly higher in low-preference cuts compared with high-preference cuts (p<0.05). Conclusion: These results may influence consumers to include more low-preference cuts in their selections based on the nutritional facts, which could help to balance the beef market in South Korea.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.270-284
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• 2021

The vomeronasal organ (VNO) is critical for reproduction and social behavior in ruminants, including cattle. The present study examined the structure of the VNO and its epithelial cells in neonatal and adult Korean native cattle (Hanwoo), Bos taurus coreanae, using immunohistochemistry and lectin histochemistry. Histologically, the VNO comprised two types of epithelia: medial vomeronasal sensory (VSE) and lateral vomeronasal non-sensory epithelia (VNSE). Numerous blood vessels and nerve bundles were observed within the vomeronasal cartilage encased lamina propria. Immunohistochemistry revealed high expression level of protein gene product9.5 and moderate expression level of olfactory marker protein in the neuroreceptor cells of the VSE and occasionally in some ciliated cells of the VNSE in both neonates and adults. The properties of the glycoconjugates in the VNO were investigated using 21 lectins, most of which were expressed at varied intensities in the VSE and VNSE, as well as in the lamina propria. Several lectins exhibited variations in their intensities and localization between neonatal and adult VNOs. This study is the first descriptive lectin histochemical examination of the VNO of Korean native cattle with a focus on lectin histochemistry, confirming that the VNO of Korean native cattle is differentiated during postnatal development.