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http://dx.doi.org/10.3347/kjp.2018.56.2.121

Detection and Identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by Molecular and Ultrastructural Studies in Naturally Infected Korean Cattle (Bos taurus coreanae) from Daejeon, Korea  

Choi, Tong-Il (College of Veterinary Medicine, Chungnam National University)
Hong, Eui-Ju (College of Veterinary Medicine, Chungnam National University)
Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University)
Sim, Cheolho (Department of Biology, Baylor University)
Chae, Joon-Seok (Laboratory of Veterinary Internal Medicine, BK21 PLUS Program for Creative Veterinary Science Research and College of Veterinary Medicine, Seoul National University)
Kim, Hyeon-Cheol (College of Veterinary Medicine, Kangwon National University)
Park, Jinho (College of Veterinary Medicine, Chonbuk National University)
Choi, Kyoung-Seong (College of Animal Science, Kyungpook National University)
Yu, Do-Hyeon (College of Veterinary Medicine, Gyeongsang National University)
Yoo, Jae-Gyu (Laboratory of Veterinary Clinics, National Institute of Animal Science Rural Development Administration)
Park, Bae-Keun (College of Veterinary Medicine, Chungnam National University)
Publication Information
Parasites, Hosts and Diseases / v.56, no.2, 2018 , pp. 121-127 More about this Journal
Abstract
To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately $0.21-1.25{\mu}m$ in length and $0.05-0.07{\mu}m$ in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.
Keywords
Sarcocystis cruzi; Bos taurus coreanae; ultrastructure; 18S rRNA;
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