Browse > Article
http://dx.doi.org/10.5352/JLS.2005.15.1.100

Effect of Mitochondrial Inhibitor on Lactate Dehydrogenase of Mesocricetus auratus and Bos taurus coreanae  

Cho Sung Kyu (Department of Life Science, Cheongju University)
Lee Sang Hak (Department of Physical Education, Korea University)
Yum Jung Joo (Department of Life Science, Cheongju University)
Publication Information
Journal of Life Science / v.15, no.1, 2005 , pp. 100-105 More about this Journal
Abstract
The lactate dehydrogenase (EC 1.1.1.27, LDH) inhibitors were isolated from the LDH-free crude mitochondrial fraction of skeletal muscle in Syrian hamster (Mesocricetus auratus) and Korean native cattle (Bos taurus coreanae). The LDH inhibitor in skeletal muscle of M. auratus was successfully isolated by the treatment with 175 mM NaCl and ultrasonic. The LDH inhibitor in skeletal muscle of B. taurus coreanae was highly stable to heat and LDH fu isozyme was largely inhibited by the LDH inhibitor. The molecular weight of inhibitor was 22 kDa. Inhibitor played an important role in the binding of LDH with the mitochondria in tissues of skeletal muscle, kidney and liver except heart.
Keywords
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fiek, C, R. Benz, N. Roos and D. Brdiczka. 1982. Evidence for identity between the hexokinase-binding protein and the mitochondrial porin in the outer membrane of rat liver mitochondria. Biochim. Biophys. Acta 688, 429-440   DOI   ScienceOn
2 Wray, W., T. Beulihas, V. P. Wray and R. Hancock, 1981. Silver staining of proteins in polyacrylamide gels. Anal. Biochem. 118, 197-203   DOI   ScienceOn
3 Lluis, C. 1984. Lactate dehydrogenase associated with the mitochondrial fraction and with a mitochomdrial inhibitorII. Enzyme interaction with a mitochondrial inhibitor. Int. J. Biochem. 16, 1005-1013   DOI   ScienceOn
4 Mattisson, A. G. M., R. G. Johansson and S. L. Bostrom. 1972. Cellular localization of lactate dehydrogenase in skeletal muscle of the eel (Anguilla anguilla). Comp. Biochem. Physiol. 41B, 474-482
5 Price., N. C. and L. Stevens. 1989. Fundamentals of Enzymology. 526pp., 2nd eds., Oxford University Press, New York
6 Vdovichenko, L. M. 1978. Some properties of lactate dehydrogenase of brain mitochondria. Ukr. Biochem. Zh. 50, 489-493
7 Ratner, J. H. and P. Nitsewojo, S. Hirway and H. O. Hultin. 1974. A study of some factors involved in the interaction between lactate dehydrogenase isozymes and particulate fractions of muscle. Int. J. Biochem. 5, 525-533   DOI   ScienceOn
8 Ross, R. E. and H. O. Hultin. 1980. A study binding-solubilization of some glycolytic enzymes in striated muscle in situ. J. Cell. Physiol. 105, 409-416   DOI
9 Skilleter, D. N. and E. Kun, 1972. The oxidation of L-lactate by liver mitochondria. Arch. Biochem. Biophys. 152, 92-104   DOI   ScienceOn
10 Whitt, G. S. 1970. Developmental genetics of the lactate dehydrogenase isozymes of fish. J. Exp. Zool. 175, 1-36   DOI   ScienceOn
11 Wilson, J. E. 1978. Ambiquitous enzymes: variations in intracellular distribution as a regulatory mechanism. Trends Biochem. Sci. 3, 124-125   DOI   ScienceOn
12 Wilson, J. E. 1982. Ambiquitous behavior of brain hexokinase. Biaphys. J. 37, 18-19   DOI   ScienceOn
13 Brdiczka, D., W. Krebs and P. Kloock. 1973. Localization of enzymes by means protease. Biochim. Biophys. Acta 297, 203-212   DOI   ScienceOn
14 Brooks, G. A., H. Dubouchaud, M. Brown, J. P. Sicurello and C. E. Butz. 1999. Role of mitochondrial lactate dehydrogenase and lactate oxidation in the intracellular lactate shuttle. Proc. Natl. Acad. Sci. U.S.A. 96, 1129-1134
15 Cho, S. K. 2000. Mitochondrial lactate dehydrogenase in tissues of vertebrate. Ph.D. Thesis Cheongju Univ., Korea 88pp
16 Cho, S. K., J. Y. Ha and J. J. Yum. 2001. Mitochondrial lactate dehydrogenase and mitochondrial inhibitor in Mesocricetus auratus, J. Ind. Sci. Cheongju Univ. 19(1), 49-54
17 Ketchum, C. H., C. A. Robinson, L. M. Hall and W. E. Grizzle. 1988. Lactate dehydrogenase isolated from human liver mitochondria: Its purification and partial biochemical characterization. Clin. Biochem. 21, 231-237   DOI   ScienceOn
18 Davis, B. J. 1964. Disc electrophoresis- II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404-427   DOI
19 Higashi, T., C. S. Richards and K. Uyeda. 1979. The interaction of phosphofructokinase with erythrocyte membrane. J. Biol. Chem. 254, 9542-9550
20 Holbrook, J. J., A. Liljas, S. J. Steindel and M. G. Rossmann. 1975. Lactate Dehydrogenase, pp. 191-292, In Boyer P. D. (eds.), The Enzymes, Academic Press Inc., New York
21 Kliman, H. J. and T. L. Steck, 1980. Association of glyceraldehyde-3-phosphate dehydrogenase with the human red blood cell membrane. J. Biol. Chem. 255, 6314-6321
22 Laemmli, U. K. 1970. Oeavage of structural proteins during the assembly of the head of bacteriophage $T_4$. Nature 227, 680-685   DOI   ScienceOn
23 Lee, S. H., S. K. Cho and J. J. Yum. 1997. Isolation and association of mitochondrial lactate dehydrogenase in Gadus macrocephalus liver. J. Ind. Sci., Cheongju Univ., Korea, 15(2), 389-397