• Title/Summary/Keyword: Bone formation

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Scientific Evidence for Autogenous Tooth Bone Graft Material (AutoBT)

  • Kim, Su-Gwan;Kim, Young-Kyun;Park, Jin-Sung
    • Journal of Korean Dental Science
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    • v.2 no.2
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    • pp.42-45
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    • 2009
  • The experimental assessment of autogenous tooth bone graft material (AutoBT) was conducted. Several studies on autogenous tooth bone graft material have confirmed the resorption of AutoBT over time and the formation of high-quality new bone.

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Scintigraphic Detection of Mammary Tumor in a Bitch (암캐에서 유전 종양의 핵의학적 진단)

  • 강성수;김명환;배춘식;최석화
    • Journal of Veterinary Clinics
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    • v.18 no.4
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    • pp.469-473
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    • 2001
  • A 12-year-old bitch, Yorkshire terrier, was admitted to the Chungbuk National University animal hospital with draining from mammary masses in the left and right mammary glands. She was involved in the mammary masses, suffering bilateral inguinal hernia. Physical examination during the hospitalization demonstrated the masses in right (3 cm) and left (1.5 cm) mammary glands. Blood pool images didn\`t show the subtle increase in blood flow to the inguinal hernia, and the increase of radionuclide distribution to the mammary gland on the soft tissue phase. However, delayed bone images showed a very focal lesion in the right mammary gland. These findings were confirmed on a subsequent biopsy, which also revealed adenocarcinoma with areas of malignant new bone formation. We present a case of primary mammary adenocarcinoma with $^{99m}Tc-MDP$ activity relative to normal bone. Tumor in the bitch demonstrates malignant new bone formation as the likely mechanism for the marked radiotracer avidity.

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The Function of Reactive Oxyegn Species in Bone Loss (골손실을 조절하는 활성산소종의 역할 규명)

  • Yang, Mi-Hye;Park, Hyo-Jung;Lee, Dong-Seok;Yim, Mi-Jung
    • YAKHAK HOEJI
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    • v.53 no.4
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    • pp.179-183
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    • 2009
  • We explored the role of reactive oxygen species (ROS) in LPS-induced bone loss. LPS was shown to increase the concentration of ROS in osteoclast precursors. The antioxidant decreased osteoclast formation by LPS. Furthermore, the antioxidant decreased NFATc1 expression by LPS, suggesting that ROS mediates NFATc1 expression in the regulation of LPS-induced osteoclast formation. Finally, the antioxidant decreased LPS-induced RANKL mRNA expression in osteoblasts. Taken together, these data indicate that LPS mediates ROS to induce bone loss.

EFFECT OF PULSED ELECTROMAGNETIC FIELD STIMULATION ON THE EARLY BONE CONSOLIDATION AFTER DISTRACTION OSTEOGENESIS IN RABBIT MANDIBLE MODEL (가토 하악골 골신장 후 맥동전자기장이 조기 골경화에 미치는 효과에 대한 연구)

  • Hwang, Kyung-Kyun;Cho, Tae-Hyung;Song, Yun-Mi;Kim, Do-Kyun;Han, Sung-Hee;Kim, In-Sook;Hwang, Soon-Jung
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.29 no.2
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    • pp.123-131
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    • 2007
  • Introduction: Distraction osteogenesis is widely used as for bone lengthening in patients with maxillofacial deformity and alveolar bone atrophy. One of the major problems in distraction osteogenesis is long consolidation period for 2-3 months, in which the devices have to be fixed on the bone to prevent relapse. It results in scar formation on the face, disturbance of mastication and speech. This study was performed to evaluate the stimulating effect of pulsed electromagnetic field on the early bone consolidation in distraction osteogenesis. Materials and methods: Total 10 rabbit were used (5 for control group, 5 for experimental group). A vertical osteotomy in the mandibular body was performed and the distraction device was fixed. After 5 days distraction was done 1mm per a day for 7 days. A pulsed electromagnetic field (38 Gauss, 60 Hz) was applied for 8 hours per day and it continued for 5 days immediately after distraction in the experimental group. Both groups were sacrificed after 2 weeks. Histological specimens with H&E and Masson Trichrome staining were made and histomorphometrically analysed with image analyser. Results: The device for distraction osteogenesis was displaced in one animal for each group, therefore, only four animals in both groups were evaluated. In both groups, a new bone formation was observed in the distracted area after 2 weeks. The bone formation was enhanced in the experimental groups ($31.76{\pm}8.68%$) compared with control group ($9.94{\pm}3.23%$), its difference was statistically significant (p<0.001). Conclusion: This study suggests that electrical stimulation with electromagnectic field may be effective in the early bone formation after distraction osteogenesis. Further studies with large number of animals are needed before clinical application.

Pulp and periodontal tissue changes following rapid tooth retraction by periodontal distraction in young adult dogs (유성견에서 periodontal distraction에 의한 급속 치아견인 시 치수 및 치주조직의 변화에 관한 연구)

  • Lee, Jong-Jin;Hong, Hyun-Sil;Chae, Jong-Moon;Jo, Jin-Hyung;Kim, Sang-Cheol
    • The korean journal of orthodontics
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    • v.37 no.5
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    • pp.351-363
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    • 2007
  • The aim of this study was to evaluate pulp and periodontal changes following rapid tooth retraction by periodontal distraction after bone undermining surgery in young adult dogs. Methods: Alter extraction of second premolars, the interseptal bone mesial to the upper 3rd premolar was undermined. After activating the distraction appliance at 0.5 mm/day for six days, the dogs were sacrificed at 0, 1, 3, 5, 7, and 9 weeks during the consolidation period. Tissue changes of periodontium and pulp were evaluated radiologically, histologically, and immunohistochemically. Results: Digital subtraction radiography showed active bone formation in the stretched periodontal ligament from 0 - 4 weeks. Resorption of the alveolar bone, appearance of osteoclasts, and infiltration of inflammatory cells were observed just after the activation period at the pressure side, and distinctive bone formation was seen in the tension side of the periodontal ligament from 1 week. New bone formation was active at 1 - 3 weeks. The expression of calcitonin gene-related peptide in the experimental group was increased at the alveolar bone and pulp, and periodontal ligament at the pressure side from 0 - 1 week, and it decreased after 5 weeks to become similar to that of the control group. Conclusions: The results showed that rapid tooth movement using periodontal distraction can be new form of orthodontic tooth movement for accelerating normal bone formation.

Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with two concentrations of expressed recombinant human bone morphogenetic protein 2

  • Choi, Hyun-Min;Park, No-Je;Jamiyandorj, Otgonbold;Choi, Kyung-Hee;Hong, Min-Ho;Oh, Seung-Han;Park, Young-Bum;Kim, Sung-Tae
    • Journal of Periodontal and Implant Science
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    • v.42 no.4
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    • pp.119-126
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    • 2012
  • Purpose: The aim of this study was to determine whether biphasic calcium phosphate (BCP) bone substitute with two different concentrations of Escherichia coli-expressed recombinant human bone morphogenetic protein 2 (ErhBMP-2) enhances new bone formation in a standardized rabbit sinus model and to evaluate the concentration-dependent effect of ErhBMP-2. Methods: Standardized, 6-mm diameter defects were made bilaterally on the maxillary sinus of 20 male New Zealand white rabbits. Following removal of the circular bony windows and reflection of the sinus membrane, BCP bone substitute without coating (control group) was applied into one defect and BCP bone substitute coated with ErhBMP-2 (experimental group) was applied into the other defect for each rabbit. The experimental group was divided into 2 subgroups according to the concentration of ErhBMP-2 (0.05 and 0.5 mg/mL). The animals were allowed to heal for either 4 or 8 weeks and sections of the augmented sinus and surrounding bone were analyzed by microcomputed tomography and histologically. Results: Histologic analysis revealed signs of new bone formation in both the control and experimental groups with a statistically significant increase in bone formation in experimental group 1 (0.05 mg/mL ErhBMP-2 coating) after a 4-week healing period. However, no statistically significant difference was found between experimental group 1 and experimental group 2 (0.5 mg/mL ErhBMP-2 coating) in osteoinductive potential (P<0.05). Conclusions: ErhBMP-2 administered using a BCP matrix significantly enhanced osteoinductive potential in a standardized rabbit sinus model. A concentration-dependent response was not found in the present study.

The Bone Formation Potency on the Titanium Cap According to the Pore on the Rabbit Calvarium (가토의 두개골에서 Pore의 유무에 따른 티타늄 반구에서의 골형성 능)

  • Park, Jung-Pyo;Oh, Chul-Jung;Jung, Seunggon;Park, Hong-Ju;Oh, Hee-Kyun;Ryu, Sun-Youl;Kook, Min-Suk
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.35 no.1
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    • pp.18-24
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    • 2013
  • Purpose: This study is performed to determine the effects of titanium cap with various sizes of pores on bone formation during guided bone regeneration (GBR). Methods: Calvaria from 10 adult male rabbits were chosen as the recipient sites. A trephine bur with a diameter of 10 mm was used to form one round groove on each side of sagittal suture of the cranium, and a round bur with a diameter of 1.5 mm was used to form 6 small holes on the inner circles of round grooves to induce bleeding. In the control group, bone graft was not conducted, and closed titanium cap was fixed in the round groove. Bone graft was not performed in groups 1 and 2, but fixed on titanium caps with 0.2 mm, and 0.5 mm sized pores, respectively. For groups 3, 4, and 5, a synthetic bone graft material (${\beta}$-tricalcium phosphate, Cerasorb$^{(R)}$, Germany) was transplanted, and titanium caps without pore, with 0.2 mm and 0.5 mm sized pore were fixed, respectively. The animals were sacrificed 4 weeks after, and clinical, radiographical, and histomorphometrical evaluation of bone regeneration was performed. Results: In all groups, there were no clinical signs of infection, inflammation or wound dehiscence. Radiographic evaluation revealed well-defined semi-circular radiopacity inside the titanium cap of groups 3, 4, and 5. Histologically, the inner surface of the hemisphere was evenly lined with newly formed bone tissue, as well as grafted bone material in the group 3. In groups 4 and 5, the insertion of connective tissue was observed along the inner surface. However, the overall surface area between the grafts with different holes yielded no statistical significance in the histomorphometrical evaluation. Conclusion: Although the total area of newly formed bone showed no significant difference, excellent bone formation tendency was observed histologically when closed caps were used with bone graft was accompanied.

EFFECT OF PLATELET-RICH PLASMA ON AUTOGENOUS BONE GRAFT FOR BONE FORMATION IN RABBIT (가토 하악골 결손부의 자가골 이식시 혈소판 풍부혈장이 골형성 촉진에 미치는 영향에 관한 연구)

  • Jeon, Min-Su;Kim, Bo-Gyun;Song, Jun-Ho;Yeon, Byong-Moo;Lee, Young-Woo;Noh, Kyung-Lok;Kim, Da-Young;Pang, Ean-O;Kim, Jun-Hyun;Nam, Jeong-Hun;Gang, Tae-In;Lim, Sung-Chul;Park, Young-Ju
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.30 no.2
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    • pp.158-164
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    • 2008
  • Purpose : Recently, various materials were developed for enhancing bone formation capacity. Platelet rich plasma(PRP) is an autologous source with several growth factors and obtained by sequestering and concentrating platelets by gradient density centrifugation. This study was to evaluate the effect of PRP on healing of grafted bone. Materials and methods : Two blood samples were obtained and analysed for measuring platelet counts of normal blood and PRP. In experimental group, two defects of mandibular bone, 10mm in diameter and 4.0mm deep, were created in the mandible and immediately grafted with autogenous bone chips mixed with PRP. In control group, same bone defects were prepared and grafted with autogenous bone chips. Gelform was used for carrier of PRP. 2 weeks, 4 weeks, 8 weeks later, each group was evaluated with histologi-cal and histomorphometric analyses. Results : According to histological observation, experimental group was showed more anastomosing newly-formed woven bone having osteoblastic activation than control group. According to histomorphometric analysis, there were 9.11% more newly-formed bone volume in experimental group than control group at 2 weeks, 7.91% more at 4 weeks, 20.08% more at 8 weeks. Conclusion: Our results demonstrated PRP in autogenous bone graft could enhance the bone formation.

Histologic study of bone-forming capacity on polydeoxyribonucleotide combined with demineralized dentin matrix

  • Kim, Seok-Kon;Huh, Chang-Kyu;Lee, Jae-Hoon;Kim, Kyung-Wook;Kim, Moon-Young
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.38
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    • pp.7.1-7.5
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    • 2016
  • Background: This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice. Methods: Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson's trichrome staining. The specimens were also evaluated via a histomorphometric study. Results: The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week. Conclusions: In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.