• Title/Summary/Keyword: Bone Characteristics

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Characteristics of Cancer Stem Cells and Immune Checkpoint Inhibition (암줄기세포의 특성 및 면역관문억제)

  • Choi, Sang-Hun;Kim, Hyunggee
    • Journal of Life Science
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    • v.29 no.4
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    • pp.499-508
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    • 2019
  • Cancer stem cells (CSCs), which are primarily responsible for metastasis and recurrence, have self-renewal, differentiation, therapeutic resistance, and tumor formation abilities. Numerous studies have demonstrated the signaling pathways essential for the acquisition and maintenance of CSC characteristics, such as WNT/${\beta}$-catenin, Hedgehog, Notch, B lymphoma Mo-MLV insertion region 1 homolog (BMI1), Bone morphogenetic protein (BMP), and TGF-${\beta}$ signals. However, few therapeutic strategies have been developed that can selectively eliminate CSCs. Recently, neutralizing antibodies against Cytotoxic T-lymphocyte associated protein 4 (CTLA-4) and Programmed cell death protein 1 (PD-1)/Programmed death-ligand 1 (PD-L1), immune checkpoint inhibitors (ICIs), have shown promising outcomes in clinical trials of melanoma, lung cancer, and pancreatic cancer, as well as in hematologic malignancies. ICIs are considered to outperform conventional anticancer drugs by maintaining long-lasting anti-cancer effects, with less severe side effects. Several studies reported that ICIs successfully blocked CSC properties in head and neck squamous carcinomas, melanomas, and breast cancer. Together, these findings suggest that novel and effective anticancer therapeutic modalities using ICIs for selective elimination of CSCs may be developed in the near future. In this review, we highlight the origin and characteristics of CSCs, together with critical signaling pathways. We also describe progress in ICI-mediated anticancer treatment to date and present perspectives on the development of CSC-targeting ICIs.

Graded levels of phytase on performance, bone mineralization and carcass traits of broiler fed reduced dicalcium phosphate

  • de Freitas, Henrique Barbosa;de Souza Nascimento, Karina Marcia Ribeiro;Kiefer, Charles;Gomes, Gilson Alexandre;Santos, Tiago Tedeschi dos;Garcia, Elis Regina Moraes;da Silva, Thiago Rodrigues;Paiva, Luanna Lopes;Berno, Patricia Rodrigues
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.5
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    • pp.691-700
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    • 2019
  • Objective: This study was conducted to assess increasing doses of phytase added to broiler diets formulated with different levels of available phosphorus (avP), calcium (Ca), and sodium (Na), and the respective effects on performance parameters, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of organs. Methods: Three different matrices were assumed for phytase with the following nutritional values: matrix A (MT A): 0.165% Ca, 0.150% avP, and 0.035% Na; matrix B (MT B): 0.215% Ca, 0.195% avP, and 0.045% Na; matrix C (MT C): 0.245% Ca, 0.225% avP, and 0.053% Na. There were six different diets: No phytase (formulated to meet the nutritional requirements); phytase 500 FTU/kg+MT A; phytase 1,000 FTU/kg+MT A; phytase 1,500 FTU/kg+MT A; phytase 1,000 FTU/kg+MT B and phytase 1,500 FTU/kg+MT C. Results: There was no significant phytase influence on performance, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of the organ throughout the study period, however, it was possible to observe a tendency of improvement in body weight corrected feed conversion for broilers fed the phytase 1,500+MT C diet, where potentially these birds were more efficient on utilize phytic phosphorus and other nutrients bounded to phytate molecule, translating into improvement in performance, and there was also a non significant numerical improvement in body weight corrected feed conversion of broilers fed this diet. Conclusion: Broilers fed with diets formulated with different levels of avP, Ca, and Na and increasing doses of phytase have shown no change on performance, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of organs.

Malignant Fibrous Histiocytoma in the Infratemporal Fossa (측두하와에 발생한 악성 섬유성 조직구성)

  • Lee Kyung-Hee;Heo Min-Suk;Lee Sam-Sun;Choi Soon-Chul
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.29 no.2
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    • pp.533-547
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    • 1999
  • Malignant fibrous histiocytoma is one of the most common soft tissue sarcomas in late adult life. but its incidence in oral and maxillofacial region is extremely rare. We report a case of malignant fibrous histiocytoma which occurred in the infratemporal fossa. Conventional radiograph of this case showed an ill-defined radiolucent lesion in the alveolar bone of the right maxillary first molar area. the lateral wall of the maxillary sinus. and the ascending ramus of mandible. MRI demonstrated a well defined mass of intermediate signal intensitiy in Tl weighted images but T2 weighted images showed two distinctive regions of different characteristics. Upper portion of the lesion was of hyperintense signal but (at) lower portion, the signal intensity decreased clearly, which might mean that this lesion(mass) is composed of two different subtypes though it couldn't be confirmed by histopathological examination. Biopsy was taken the lesion as only in the soft tissue of the maxillary posterior alveolar region and confirmed the storiform-pleomorphic type of malignant fibrous histiocytoma. Histopathological subtype was well consistent with the relatively aggressive imaging findings of that region. We expect more detailed analysis of the nature of malignant fibrous histiocytoma with improvement of the imaging modality and the identification of the relationship between diagnostic imaging and histopathologic findings.

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The growth stage study on the femur and tibia of Korean native chicken after hatching (한국 재래닭의 부화 후 성장단계별 넙다리뼈와 정강뼈 성장에 관한 연구)

  • Tae, Hyun-Jin
    • Korean Journal of Veterinary Service
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    • v.38 no.3
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    • pp.181-188
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    • 2015
  • Skeletal development of chicken has been widely discussed in industrial forums and various research reports. However, these studies were emphasis on the commercial chicken strains for improve egg and meat production whereas the skeletal quiet remains as a potential weak link related to facilitating in the physical support of heavier carcasses at ever younger ages. For that, the study of standardization of skeletal development is important but it was rarely reported in Korean native chicken (KNC). The study was investigated the skeletal characteristics of KNC for international standardization. We studied in KNC at 2, 14, 28, 42, 56, 70, 84, 98, 112, 126, 147, 168, 196, 224, 336 and 448 days after hatch (male and female, n=13 for each group). We measured the body weight (BW), and after sacrifice measured organs and remove muscle from femur & tibia and measured bone weight. Data were analyzed by ANOVA, Duncan test, correlation analysis and regression analysis of SAS 9.1. We analyzed the data of BW, femur & tibia and made growth curve also. The BW was significantly increased up to 147 days after hatch (male, $1,927.88{\pm}68.92g$; female, $1,456.00{\pm}50.11g$), and then increased gradually. At 336 days, these growth was stop (male, $2,467.00{\pm}42.84g$; female, $1,568.71{\pm}62.62g$). The growth of femur & tibia length and width was stop on 98~126 days after hatch. At 98 days, we measured the length and width of femur & tibia in male were $132.39{\pm}3.18mm$ & $25.98{\pm}0.59mm$ whereas in female at 112 days the length of femur & tibia was $116.40{\pm}1.55mm$ and at 126 days width was $21.41{\pm}0.38mm$. Our study suggests that the growth of male KNC was classified pre-puberty (0~98 days), puberty (98~336 days) and maturity (after 336 days), meanwhile female was shown similar trend however puberty period of KNC was 112 or 126 days after hatch.

Two cases of an atypical presentation of necrotizing stomatitis

  • Magan-Fernandez, Antonio;O'Valle, Francisco;Pozo, Elena;Liebana, Jose;Mesa, Francisco
    • Journal of Periodontal and Implant Science
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    • v.45 no.6
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    • pp.252-256
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    • 2015
  • Purpose: The purpose of this report was to describe the clinical and microbiological characteristics of two rare cases of necrotizing stomatitis, and the outcomes of a non-invasive treatment protocol applied in both cases. Methods: We report two cases of necrotizing stomatitis in a rare location in the hard palate of a 40-year-old woman and a 28-year-old man. Neither had a relevant medical history and both presented with highly painful ulceration in the palate and gingival margin that was accompanied by suppuration and necrosis. 3% hydrogen peroxide was applied to the lesions using sterile swabs, and antibiotic and anti-inflammatory treatment was prescribed to both patients in addition to two daily oral rinses of 0.2% chlorhexidine. Results: In both cases, radiological examination ruled out bone involvement, and exfoliative cytology revealed a large inflammatory component and the presence of forms compatible with fusobacteria and spirochetes. There was a rapid response to treatment and a major improvement was observed after 48 hours, with almost complete resolution of the ulcerated lesions and detachment of necrotic areas with partial decapitation of gingival papillae. Conclusions: Necrotizing periodontal lesions can hinder periodontal probing and the mechanical removal of plaque in some cases due to the extreme pain suffered by the patients. We present a non-invasive treatment approach that can manage these situations effectively.

Implementation of Fitting Software for Fully Implantable Middle Ear Hearing Device (완전 이식형 인공중이용 적합 소프트웨어의 구현)

  • Lee, J.W.;Jung, E.S.;Lim, H.K.;Lee, J.H.;Seong, K.W.;Kim, M.N.;Cho, J.H.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.3 no.1
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    • pp.21-25
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    • 2009
  • Generally, fully implantable middle ear hearing device (F-IMEHD) is implanted under the skin nearby human temporal bone with all components including implantable microphone and vibration transducer. The microphone and transducer have different characteristic before and after implant. Fitting process is performed for this characteristic change of them and proper performance of hearing aids for each patient. Conventional hearing aids and partially implantable hearing aids, they have wired connector for fitting process. However in case of F-IMEHD, it is difficult this wired connection, because all components of F-IMEHD is implanted. In this paper, fitting software that can be apply wireless fitting hardware for F-IMEHD has been designed and implemented. It can find out proper fitting parameter reflecting characteristics of the microphone and transducer for patients who has difficulty in hearing.

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Effects of Method and Time of Castration on Growth and Carcass Characteristics of Omani Sheep

  • Mahgoub, O.;Horton, G.M.J.;Olvey, F.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.2
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    • pp.121-127
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    • 1998
  • Thirty two Omani sheep with eight animals each of; intact males (INT), males castrated with rubber rings immediately after birth (RR), males castrated with a burdizzo at 8 weeks of age (BC) and intact females (IF) were fed ad libitum a concentrate diet (CP 16%) plus chopped Rhodesgrass hay (8% CP) from weaning until slaughter at 28 kg. INT lambs grew faster from 9 to 20 weeks of age (p < 0.05) thus they were significantly heavier at 20 weeks of age than BC and IF, but not RR lambs. INT consumed more total feed than other sex groups over the period from 9 to 20 weeks of age. There were no significant differences between lambs of all experimental groups in feed per gain ratio. INT lambs had lower (p < 0.01) dressing percentage (DP) than RR, BC and IF. As a percentage in the empty body weight (EBW), INT had higher proportions of head, feet, empty gut (p < 0.001), liver (p < 0.05) and genitals (p < 0.05) but lower proportions of lungs and trachea (p < 0.05) than BC and IF lambs. INT males had a significantly higher (p < 0.05) proportion of bone than RR and IF lambs but lower (p < 0.05) proportion of fat than RR and IF. As a percentage in EBW, IF had the highest protortion (p < 0.001) of total body fat (TBF) followed by BC and RR whereas INT males had the lowest proportion. There was a general trend of IF having the highest proportion of individual and total non-carcass fat (TNCF) and total carcass fat (TCF) followed by BC and RR lambs whereas INT lambs had the lowest protortions of individual carcass and non-carcass fat depots. There were only few sex or castration effects on carcass tissue distribution. IF had higher proportions of intermuscular fat in the chuck, plate, leg and flank than INT and BC. The current study demonstrated that castration of intensively-raised male Omani native sheep especially at weaning using a burdizzo retarded growth rate and reduced carcass quality by increasing fat content.

Effectiveness of digital subtraction radiography in detecting artificially created osteophytes and erosions in the temporomandibular joint

  • Kocasarac, Husniye Demirturk;Celenk, Peruze
    • Imaging Science in Dentistry
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    • v.47 no.2
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    • pp.99-107
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    • 2017
  • Purpose: Erosions and osteophytes are radiographic characteristics that are found in different stages of temporomandibular joint (TMJ) osteoarthritis. This study assessed the effectiveness of digital subtraction radiography (DSR) in diagnosing simulated osteophytes and erosions in the TMJ. Materials and Methods: Five intact, dry human skulls were used to assess the effectiveness of DSR in detecting osteophytes. Four cortical bone chips of varying thicknesses (0.5 mm, 1.0 mm, 1.5 mm, and 2.0 mm) were placed at the medial, central, and lateral aspects of the condyle anterior surface. Two defects of varying depth (1.0 mm and 1.5 mm) were created on the lateral, central, and medial poles of the condyles of 2 skulls to simulate erosions. Panoramic images of the condyles were acquired before and after artificially creating the changes. Digital subtraction was performed with Emago dental image archiving software. Five observers familiar with the interpretation of TMJ radiographs evaluated the images. Receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic accuracy of the imaging methods. Results: The area under the ROC curve (Az) value for the overall diagnostic accuracy of DSR in detecting osteophytic changes was 0.931. The Az value for the overall diagnostic accuracy of panoramic imaging was 0.695. The accuracy of DSR in detecting erosive changes was 0.854 and 0.696 for panoramic imaging. DSR was remarkably more accurate than panoramic imaging in detecting simulated osteophytic and erosive changes. Conclusion: The accuracy of panoramic imaging in detecting degenerative changes was significantly lower than the accuracy of DSR (P<.05). DSR improved the accuracy of detection using panoramic images.

PERIPHERAL, SOFT TISSUE ODONTOMA : CASE REPORT (Peripheral, soft tissue odontoma에 관한 증례보고)

  • Lee, Kwang-Chul;Choi, Hyung-Jun;Choi, Byung-Jae;Lee, Jong-Gap
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.1
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    • pp.157-161
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    • 1999
  • Odontomas, hamartomas of odontogenic origin, are composed of all the structures that make up teeth. The WHO distinguishes odontoma into two types. The complex odontoma is defined as "a malformation in which all the dental tissues are represented, individual tissues being mainly well-formed but occurring in a more or less disorderly pattern." The compound odontoma is defined as "a malformation in which all the dental tissues are represented in a more orderly pattern than in the complex odontoma, so that the lesion consists of many toothlike structures. Most of these structures do not resemble morphologically the teeth of normal dentition, but in each one enamel, dentine, cementum, and pulp are arranged as in the normal tooth." Almost all odontomas are located intraosseously, but they have occasionally been reported in extrabony location. Peripheral or soft tissue odontomas, those arising outside of the alveolar bone, are very rare. Peripheral or soft tissue odontoma are defined as tumors that demonstrate the histologic characteristics of their intraosseous counterparts but occur solely in the soft tissue covering the tooth-bearing portion of the mandible and maxilla. When they mature, they appear as a radiopaque mass without the peripheral halo. The final diagnosis should be confirmed by biopsy. The origin of peripheral odontoma is probably related to remnants of the dental lamina in the gingiva. The treatment of choice is complete surgical excision, similarly to intraosseous odontoma and it does not tend to recur. This report presents a case of 5-year-old boy with swelling on labial gingiva of primary central incisor. And it was diagnosed as peripheral odontoma by excisional biopsy.

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Effect of $17{\beta}$-Estradiol and 1,25-Dihydroxyvitamin $D_3$ on Interleukin-6 Production of Periodontal Ligament Cells (($17{\beta}$-Estradiol 및 1,25-Dihydroxyvitamin $D_3$가 치주인대 세포의 Interleukin-6의 생성에 미치는 영향)

  • Kwak, Wall-Ah;Choi, Bong-Kyu;Lee, Hyun-Jung;Yoo, Yun-Jung
    • Journal of Periodontal and Implant Science
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    • v.29 no.3
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    • pp.645-654
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    • 1999
  • Interleukin-6(IL-6) stimulate osteoclast differentiation. $17{\beta}$-estradiol, 1,25-dihydroxyvitamin $D_3$(1,25-$(OH)_2D_3$) and interleukin-$1{\beta}$ inhibit or stimulate osteoclast differentiation by decreasing or increasing the synthesis of interleukin-6(IL-6) from stromal/osteoblastic cells, respectively. Periodontal ligament(PDL) cells reside between the alveolar bone and the cementum and have osteoblastic characteristics. To estimate the effect of $17{\beta}$-estradiol and 1,25$(OH)_2D_3$ on IL-6 production of PDL cells, PDL cells were treated with $17{\beta}$-estradiol or 1,25-$(OH)_2D_3$ in the absence or the presence of IL-$1{\beta}$. The concentration of IL-6 produced form PDL cells was determined by enzym linked immunosorbent assay(ELISA). In unstimulated PDL cells, we detected constitutive production of IL-6 at 1st and 2nd day. IL-$1{\beta}$ increased IL-6 synthesis at 1st day and 2nd day. $17{\beta}$-estradiol had no significant effect on the secretion of this cytokine, either constitutively or after stimulation with IL- $1{\beta}$(0.05 ng/ml). 1,25-$(OH)_2D_3$($10^{-8}M$) decreased not only constitutive IL-6 production but also IL-$1{\beta}$-induced IL-6 production at 2nd day. These results suggest that 1,25-$(OH)_2D_3$ may control IL-$1{\beta}$-induced osteoclast differentiation by decreasing IL-$1{\beta}$-induced IL-6 secretion of PDL cells.

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